共查询到18条相似文献,搜索用时 93 毫秒
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高效液相色谱法测定野菊花中蒙花苷的含量 总被引:2,自引:1,他引:2
建立高效液相色谱法测定野菊花中蒙花苷含量的方法。采用KromosilTM C18十八烷基硅烷键合硅胶色谱柱,甲醇-水-乙酸(体积比为55∶44.5∶0.5)为流动相,流速为0.8mL/min,柱温为室温,检测波长为334nm。蒙花苷含量在11.2~89.6μg/mL范围内,线性关系良好(r=0.9998),方法的相对标准偏差为0.51%(n=5),平均加标回收率为98.92%。 相似文献
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气相色谱法测定芦荟及芦荟干粉中的多糖 总被引:4,自引:0,他引:4
用气相色谱法测定了芦荟及芦荟干粉样品中的多糖。实验以木糖醇为内标物,采用以OV-225与OS-138为固定液的色谱柱,使多糖水解为单糖后的衍生物得到了分离。结果表明:未经水解的芦荟中含有少量的单糖,主要为葡萄糖;水解后的单糖主要为甘露糖和葡萄糖。这与用乙醇沉淀多糖后的测定结果(即芦荟及芦荟干粉中的多糖主要为甘露聚糖和葡甘露聚糖)是一致的。 相似文献
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建立反相高效液相色谱法测定苁蓉酒中松果菊苷含量。固定相为C18,流动相为甲醇-0.1%甲酸水溶液(体积比为33:67),流速1.0mL/min,柱温为30℃,检测波长为330nm。松果菊苷的浓度x(mg/mL)与其峰高y呈良好的线性关系,线性方程为Y=2.5955x,相关系数为0.9999。样品的加标回收率为88.1%~107.5%,测定结果相对标准偏差为0.41%(n=5)。 相似文献
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高效毛细管电泳安培法测定芦荟中芦荟苷的含量 总被引:1,自引:0,他引:1
建立了芦荟中芦荟苷含量的高效毛细管电泳安培检测方法. 探讨了缓冲溶液种类、浓度、酸碱度,工作电位,有机改性剂及其操作电压、进样时间等对检测的影响. Na2B4O7-NaOH(Na2B4O7浓度为30 mmol/L)为缓冲液,工作电位为-0.8 V,体积分数为2.5%甲醇为有机添加剂,进样时间为10 s,在15 kV分离高压,pH=9.5的碱性条件下柱端安培法检测了芦荟苷的含量. 该法的线性范围为50~0.05 mg/L,线性方程Y=856.2+123.3c,相关系数r=0.999 4,检出限为0.01 mg/L. 相似文献
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L-半胱氨酸衍生物配体交换手性色谱固定相 总被引:13,自引:0,他引:13
应用密闭微波萃取装置对芦荟中的有效成分芦荟甙进行了微波萃取研究,并利用透射电子显微镜对微波萃取机理进行了初步探讨,讨论了不同萃取剂、溶剂浓度、萃取时间和微波功率等对提取率的影响,在萃取剂为乙醇-水体系,溶剂(乙醇)体积分数为70%、萃取时间为4min及微波功率为340W的条件下,萃取效果最佳,与索氏提取及超声波萃取法相比,本法具有萃取速度快、提取率高及溶剂用量少等特点。 相似文献
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A spectrophotometric method for quantitative determination of total content of phenolic compounds in leaves of Aloe arborescens Mill. (Liliaceae) calculated as aloenin was developed. Statistical analysis showed that the relative uncertainty of the determination
method was less than 2%.
Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 578–580, November–December, 2008. 相似文献
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Kamil Pawowicz Dominika Ludowicz Marta Kara
niewicz-ada Kamil Wdowiak Judyta Cielecka-Piontek 《Molecules (Basel, Switzerland)》2021,26(11)
The aim of the study is to evaluate the composition of lyophilisates obtained from Aloe arborescens leaf gel at the age of one to four years. The leaves were obtained from controlled crops, which allowed to exclude environmental factors as variables. It was confirmed that the lyophilisates obtained from different years of Aloe arborescens leaf gel varied in chromatographic analyses in terms of aloin A and aloenin A content (high-performance liquid chromatography with diode-array detection HPLC-DAD, high-performance liquid chromatography with tandem mass spectrometric detection HPLC-MS/MS). Similarly, while testing the phenolic acids and the sum of polyphenols content, differences in their levels in leaf gel lyophilisates from plants of individual years were observed (spectrophotometric method UV-VIS). The lyophilisate composition analysis showed that the one-year-old leaves were characterized by the highest content of aloin A and aloenin A. While the content of polyphenols, including phenolic acids, was higher in the leaves of older plants. The antioxidant potential of the tested lyophilisates was assessed simultaneously. Regardless of the research model used (CUPRAC, DPPH, ABTS), an antioxidant effect was noted for Aloe arborescens leaves. 相似文献
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Kamil Pawowicz Szymon Sip Tomasz Plech Barbara Kapro Joanna Kobus-Cisowska Judyta Cielecka-Piontek 《Molecules (Basel, Switzerland)》2022,27(7)
The present study assessed the genotoxicity, the possibility of inhibiting selected enzymes, and the microbial activity of lyophilisate from 3-year-old A. arborescens leaves obtained from controlled crops. The lyophilisate from 3-year-old A. arborescens leaves was standardized for aloin A and aloenin A content. Moreover, concentrations of polyphenolic compounds and phenolic acids were determined. The first stage of the research was to determine genotoxicity using the comet test, which confirmed the safety of A. arborescens. Assays of enzymatic inhibition were performed for hyaluronidase (IC50 = 713.24 ± 41.79 µg/mL), α-glucosidase (IC50 = 598.35 ± 12.58 µg/mL), acetylcholinesterase and butyrylcholinesterase (1.16 vs. 0.34 µM of eserine/g d.m., respectively). The next stage of the research was to determine the ability of the healing properties using the scratch test, which showed a positive response using the extract. Microbial activity was evaluated and obtained against Gram-negative and Gram-positive bacteria and yeasts. We concluded that A. arborescens leaf gel meets the important conditions for plant raw materials to obtain semi-solid forms of herbal medicinal products. 相似文献
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Fe3+-H2O2-二氯荧光素化学发光体系测定药物中的扑热息痛 总被引:1,自引:0,他引:1
酸性介质中,Fe3+催化H2O2分解生成羟基自由基,进而氧化扑热息痛产生微弱的化学发光,二氯荧光素对该发光强度有较强的增敏作用。研究了影响化学发光强度的各种因素,并探讨了其可能的发光机理。在最佳化学发光条件下,其化学发光强度与扑热息痛的浓度在8.0×10-8~5.0×10-5mol/L范围内呈良好的线性关系,检出限为5.0×10-9mol/L,对3.5×10-6mol/L的扑热息痛平行测定9次,其相对标准偏差为2.2%。该法用于片剂中扑热息痛含量的测定,结果满意。 相似文献
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用火焰原子吸收光谱法测定了芦荟中铁、锌两种微量元素含量。结果表明,芦荟中铁和锌千含量分别为638、244μg/g。火焰原子吸收光谱法测定样品中铁含量和锌含量的相对标准偏差分别为7.06%,3.63%,加标回收率在95%~102.5%范围内,检测限分别为0.011μg,/mL和0.002μg/mL。 相似文献