STUDY OF THE FLUORESCENCE SYSTEM OF RIBONUCLEIC ACID－Tb（Ⅲ） AND THE DETERMINATION OF RIBONUCLEIC ACID

ＳＴＵＤＹＯＦＴＨＥＦＬＵＯＲＥＳＣＥＮＣＥＳＹＳＴＥＭＯＦＲＩＢＯＮＵＣＬＥＩＣＡＣＩＤ－Ｔｂ（Ⅲ）ＡＮＤＴＨＥＤＥＴＥＲＭＩＮＡＴＩＯＮＯＦＲＩＢＯＮＵＣＬＥＩＣＡＣＩＤ￥ＪｉｎｇＨｅＹＡＮＧ；ＣｈａｎｇＬｕｎＴＯＮＧ；ＮｉａｎＱｉｎＪＩＥ；Ｈｏ...     

THE STUDY OF INTERACTION OF SOLID-LIQUID ADSORPTION SYSTEM BY USING THE FLOW INJECTION-SPECTROPHOTOMETRY SYSTEM

1. INTRODUCTION Flow injection analysis is a method that a determinate volume of sample is injected to an airless and continuous flow carrier consisted of suitable liquids, and then is put into a detector to be record absorbency, electrode potential and other physical parameters. This automatic analytical method is characterized by fixed time, quantified sampling, less sampling and good system stability [1]. The new automatic analysis for liquid has been developed quickly since Ruzi…     

OXIDATION OF Tb(Ⅲ)IN THE HETEROPOLY-TUNGSTOPHOSPHATE COMPLEX

It has first been reported that Tb(Ⅲ)is oxidated by K_2S_2O_8 in the heteropoly-tungstophosphate complex.The complex ion,[Tb(Ⅳ)(PW_(11)O_(39))_2]~(10-),is stable in aqueous solutiononly for hours,being graduately reduced to[Tb(Ⅲ)(PW_(11)O_(39))_2]~(11-).It is red-brown,with absorptionband at about 420 nm.     

STUDIES ON DERIVATIVE FLUORIMETRY Ⅲ. DETERMINATION OF TRACE AMOUNT OF TERBIUM IN THE PRESENCE OF SODIUM DODECYL SULFONATE

The derivative fluorimetric characteristics of Tb—SDS and Tb—SDS—phenanthroline systems have been studied. The optimum conditions were obtained. The 2nd—derivative spectrofluorimetric method in the systems has been applied to the determination of trace amounts of terbium, in rare earth ore and their oxides. The results are satisfactory.     

A QUENCHING FLUORESCENCE IMMUNOASSAY METHOD FOR DETERMINATION OF TRACE ALBUMIN USING UNLABELED TERBIUM CHELATE

A fluoroimmunoassay method using unlabeled Terbium chelate isdescribed.The principle is similar to that of fluoroimmunoassay method usinglanthanide chelate as labels.The procedure is simpte because labeling processis unnecessary.The recovery of HSA and albumin in urine is 107% and95% respectively.The standard deviation is tess than 10%.     

THE DETERMINATION OF GLUCOSE IN URINE BY USING CYCLIC VOLTAMMETRY

ＴＨＥＤＥＴＥＲＭＩＮＡＴＩＯＮＯＦＧＬＵＣＯＳＥＩＮＵＲＩＮＥＢＹＵＳＩＮＧＣＹＣＬＩＣＶＯＬＴＡＭＭＥＴＲＹ￥ＺｈｅｎｇＹｕＺＨＯＵ；ＣａｎＺｈｕＧＡＯ；ＱｉｎｇＣｕｎＺＨＵ；ＦｕＹｅＧＡＯ；ＦａｎＺｈｅｎＫＯＮＧ（ＣｈｅｍｉｓｔｒｙＤｅｐａｒｔ...     

THE STUDY OF INTERACTION OF SOLID—LIQUID ADSORPTION SYSTEM BY USING THE FLOW INJECTION—SPECTROPHOTOMETRY SYSTEM

The flow injection analysis was firatly used for studying a solid-liquid adsorption system,and the dynamics process in the adsorption of adyestuff with regenerable chitin was traced by an online method of flow injection-spectrophotometry,Experimental results indicate that there is a linearization between the tested signals and the height of peaks with reciprocity coefficient 0.9999 by using the flow injection-spectrophotometry system to study the dynamics adsorption process in solid-liquid system.The method shows a good stability and reproducibility.It provides a new method for the studies on adsorption dynamics in solid-liquid system.     

CHRONOABSORPTOMETRY FOR THE DETERMINATION OF KINETIC PARAMETERS OF ELECTRON TRANSFER REACTIONS USING LONG-OPTICAL-PATH ELECTROCHEMICAL CELL

A single potential step chronoabsorptometric method for the determination of ki-netic parameters of simple quasi-reversible reactions is described.It is verified by determiningthe kinetic parameters for the electroreduction of ferricyanide.A long-optical-path electro-chemical cell with a plug-in electrode is used.The thickness of solution layer is 0.55 mm     

A STUDY ON THE BEHAVIOUR AND MECHANISM OF EXTRACTING GOLD(Ⅲ)USING PTSO

PTSO is abbreviated from di-P-tolyl Eolphoxide having a>S=0 group and is a neutral extractant.We have studied the behaviour and conditions of the separation of Au(Ⅲ) from other elements on a column packed with the silica gel loaded with PTSO and have found that PTSO is a new stationary phase with extremely high selectivity for the separation and concentration of gold.However,the application of PTSO to the solvent extraction has not bean reported yet.We first studied the behaviour and mechanism using PTSO to extract gold by atomic-absorption spectrophotometry,ultra-violet spectrophotometry and infra-red spectrophotometry.The possible structure of the extractive formed from PTSO and Au(Ⅲ) is suggested.     

SPECTROFLUORIMETRIC DETERMINATION OF GERMANIUM WITH MORIN IN WATER AND HEALTH DRINK

A highly sensitive and fairly selective spectrofluorimetric method has been developed for the determination of germanium with morin in tap water and health drink. The fluorescent reaction and optimal conditions of germanium with morin in phosphoric acid medium were studied. The detection limits of germanium in tap water and health drink were found to be 0.2 and 0.7μd/L respectively.     

SPECTROSCOPIC DETERMINATION OF THE AVERAGE NUMBER OF COAGGREGATES (Nco) OF CHOLESTERYL ESTERS AND THE FLUORESCENCE PROBE

Average aggregate number of coaggregates(N_co)of CE-n or BL-n and the fluoresc-ence probe(Np-16)have been determined by using time-resolved fluorescence spectroscopy.Chain-length,hydroxy-group and chain-foldability effects on the N_co have been discussed.     

β－CORRECTION SPECTROPHOTOMETRIC DETERMINATION OF ALUMINIUM， USING THE AL（Ⅲ）－PF－OP REACTION

β－ＣＯＲＲＥＣＴＩＯＮＳＰＥＣＴＲＯＰＨＯＴＯＭＥＴＲＩＣＤＥＴＥＲＭＩＮＡＴＩＯＮＯＦＡＬＵＭＩＮＩＵＭ．ＵＳＩＮＧＴＨＥＡＬ（Ⅲ）－ＰＦ－ＯＰＲＥＡＣＴＩＯＮ￥ＨｏｎｇＷｅｎＧＡＯ；ＦｅｎｇＺＯＵａｎｄＰｅｎｇＦｅｉＺＨＡＮＧ（ＨｕａｉｂｅｉＥ...     

KINETIC AND APPLICATION: STUDIES ON THE REACTION SYSTEM OF OSMIUM(Ⅳ)-ARSENIC(Ⅲ)-CHLORATE

The experiments have proved that Os(Ⅳ) has very strong catalytic effect on the chlorate oxidation of As(Ⅲ) in perchloric acid media, and As(Ⅲ) exhibits a sensitive single-sweep oscillopolarographic wave at-0.75 V vs. SCE. The kinetics of this catalytic reaction has been investigated by the initial rate procedure. The mechanism has been proposed. A new and highly sensitive catalytic reaction-oscillopolarographic method for the determination of ultratrace amounts of osmium has been described, based on the Os(Ⅳ) catalytic effect on the reaction. The linear range of 5.0×10~(-11)-4.4×10~(-9) mol/L and 6.0×10~(-9)-1.3×10~(-7) mol/L Os was obtained using the fixed-time procedure and the initial rate procedure, respectively. Osmium in refined ore was analysed by this method.     

LACCASE－BASED MICELLAR ENHANCED SPECTROFLUORIMETRIC DETERMINATION OF L－ASCORBIC ACID

ＬＡＣＣＡＳＥ－ＢＡＳＥＤ ＭＩＣＥＬＬＡＲ ＥＮＨＡＮＣＥＤ ＳＰＥＣＴＲＯＦＬＵＯＲＩＭＥＴＲＩＣ ＤＥＴＥＲＭＩＮＡＴＩＯＮ ＯＦ Ｌ－ＡＳＣＯＲＢＩＣ ＡＣＩＤ￥ＨｏｕＰｉｎｇＨＵＡＮＧ；ＲｕＸｉｕＣＡＩ；ＹｕＭｉｎＤＵａｎｄＹｕｎＥＺＥＮＧ...     

DETERMINATION AND REFINEMENT OF THE CRYSTAL STRUCTURE OF CHAIDAMUITE

Chaidamuite (ZnFe(SO_4)_2(OH). 4H_2O), which is a new sulphate mineral, belongs to the triclinic system, space group P1, with the cell parameters corrected by the least-squares method: a=7.309(2), b=7.202(2), c=9.691(3), α=89.64(3), β=105.89(3), γ=91.11(2)°, Z=2. The crystal structure has been determined by the Patterson method and Fourier syntheses and refined by the full-matrix least-squares method to an R factor of 0.032, using 2833 independent reflections. In the structure, a zigzag chain consists of [Fe(1)O_5(OH)] and [Fe(2)O_5(OH)] octahedra sharing the OH corners, and an octahedral-tetrahedral chain running parallel to the b axis consists of the zigzag chain of Fe octahedra and (SO_4) tetrahedra sharing four pairs of octahedral corners on either side of the zigzag chains. These chains are cross-linked by the isolated [Zn(1)O_2(H_2O)_4] and [Zn(2)O_2(H_2O)_4] octahedra into corrugated sheets parallel to the (100) plane. Adjacent sheets are hydrogenbonded through water molecules.     

THE EXPERIMENTS OF SEASONAL PREDICTION USING THE ANALOGY-DYNAMICAL MODEL

From the viewpoint of dynamics, it is convenient to regard the field to be predicted as a small disturbance superposed on the historical analogous field, and thus the statistical technique can be used in combining with the dynamics. Along this line, a coupled atmosphere-earth surface analogy-dynamical model is formulated and applied to making eight seasonal predictions. All of the predictions were initiated from January and have been made from February to August of 1981 to 1988. The experiments of eight-year predictions show certain skill in seasonal prediction, and the skill scores of prediction are greater than those of single statistical analogy forecast.     

DETERMINATION OF SERUM ALBUMIN USING A SILVER ION SELECTIVE ELECTRODE

ＤＥＴＥＲＭＩＮＡＴＩＯＮＯＦＳＥＲＵＭＡＬＢＵＭＩＮＵＳＩＮＧＡＳＩＬＶＥＲＩＯＮＳＥＬＥＣＴＩＶＥＥＬＥＣＴＲＯＤＥ￥ＦｅｎｇＬｉａｎＬＵ（ＤｅｐａｒｔｍｅｎｔｏｆＣｈｅｍｉｓｔｒｙ，ＺｈｅｎｇｚｈｏｕＵｎｉｖｅｒｓｉｔｙ，Ｚｈｅｎｇｚｈｏｕ，４...     

DETERMINATION OF PHASE INVERSION POINT IN THE EMULSIFICATION OF POLYMERS

Polymer Physics Laboratory, Institute of Chemistry,The Chinese Academy of Sciences, Beijing 100080, China     

THE GROWTH OF SINGLE CRYSTAL AND THE DETERMINATION OF CRYSTALLOGRAPHIC PA RAMETERS OF (L-TRYPTOPHAN)~(A1)-INSULIN AND (D-TRYPTOPHAN)~(A1)-INSULIN

The crystal-growing conditions and the results of preliminary X-ray crystallographic analysis of (L-Try)~(A1)-insulin and (D-Try)~(A1)-insulin are reported. The single crystals of this pair of insulin analogue suitable for X-ray diffraction analysis have been grown in the citrate buffer system by still-setting method. They both belong to the trigonal system with space group R3. The parameters of the unit cell (L-Trp)~(A1)-insulin are αH= 80.31, cH= 37.45 and those of (D-Trp)~(A1)-insulin αH = 79.48, cH = 43.81. There are two molecules in an asymmetric unit. The obtained results are discussed.     

USE OF ANNULAR ARTIFICIAL PUPIL IN THE DETERMINATION OF MODULATION TRANSFER FUNCTION OF THE EYE

According to Bef. [1] primary spherical aberration of the schematic eye would be com-pletely absorbed by an annular aperture when its obstruetion ratio ∈≥0.60. Consequently thecontrast sensitivity function, T_(cs), determined with the use of such an annulus, T_(cs) (A), is theMTF of the retina-brain system, T_(rh), weighted by the MTF of the annulus, T_⊙.T_(rh) thus obtain-ed can be used to determine the MTF of the eye, T_e. Annular apertures of various sizes andobstruction ratios used for the determination of T_e have been analysed. It is shown that thereare several factors other than incomplete absorption of primary spherical aberration thatwould prevent the use of annular pupil to derive T_(rb) under unrestrained conditions. This is dueto the fact that the apparent T_⊙'s of annuli used in the actual determination of T_(cs)(A) usual-ly deviate from the theoretical T_⊙'s for the passive eye to different extents. However, it isfound that the T_⊙ for annulus of o.d.=2.7 mm and ∈= 0.60 conforms more or less to thetheoretical T_⊙ and can be used to derive reasonably accurate T_e(2.7). It is also possibleto estimate T_e(>2.7) from T_e(2.7) by some empirical correction.