In‐line coupled single drop liquid–liquid–liquid microextraction with capillary electrophoresis for determining fluoroquinolones in water samples

A simple in‐line single drop liquid–liquid–liquid microextraction (SD‐LLLME) coupled with CE for the determination of two fluoroquinolones was developed. The method is capable to quantify trace amount of analytes in water samples and to improve the sensitivity of CE detection. For the SD‐LLLME, a thin layer of organic phase was used to separate a drop of 0.1 M NaOH hanging at the inlet of the capillary from the aqueous donor phase. By this way, the analytes were extracted to the acceptor phase through the organic layer based on their acidic/basic dissociation equilibrium. The drop was immersed into the organic phase during 10 min for extraction and then it is directly injected into the capillary for the analysis. Parameters such as type and volume of organic solvent phase, aqueous donor, and acceptor phases and extraction time and temperature were optimized. The enrichment factor was calculated, resulting 40‐fold for enrofloxacin (ENR) and sixfold for ciprofloxacin (CIP). The linear range were 20–400 μg/L for ENR and 60–400 μg/L for CIP. The detection limits were 10.1 μg/L and 55.3 μg/L for ENR and CIP, respectively, and a good reproducibility was obtained (4.4% for ENR and 5.6% for CIP). Two real water samples were analysed applying the new method and the obtained results presented satisfactory recovery percentages (90–100.3%).     

Miniaturized matrix solid‐phase dispersion coupled with supramolecular solvent‐based microextraction for the determination of paraben preservatives in cream samples

An analytical method is presented for the determination of paraben preservatives in semisolid cream samples by matrix solid‐phase dispersion combined with supramolecular solvent‐based microextraction. Due to the oily and sticky nature of the sample matrix, parabens were first extracted from the samples by matrix solid‐phase dispersion using silica as sorbent material with a clean‐up performed with tetrahydrofuran in the elution step. The eluate (500 μL), 1‐decanol (120 μL), and water (4.4 mL) were then mixed in a polyethylene pipette to form supramolecular solvent. Finally, the analytes in the supramolecular solvent were separated and determined by liquid chromatography with ultraviolet detection. Under optimal extraction conditions, the extraction recoveries of the studied compounds were obtained in the range of 63–83%. The limits of detection for the analytes were between 0.03 and 0.04 μg/g. The precision of the method varied between 4.0–6.7 (intraday) and 6.2–7.9% (interday). Finally, the optimized procedure was applied to the determination of the target preservatives in a variety of cream samples (diaper rash, skin allergy, face and hand moisturizing) with satisfactory recoveries (86–102%).     

Temperature‐controlled liquid–liquid microextraction combined with high‐performance liquid chromatography for the simultaneous determination of diazinon and fenitrothion in water and fruit juice samples

A simple, environmentally benign, and rapid method based on temperature‐controlled liquid–liquid microextraction using a deep eutectic solvent was developed for the simultaneous extraction/preconcentration of diazinon and fenitrothion. The method involved the addition of deep eutectic solvent to the aqueous sample followed by heating the mixture in a 75°C water bath until the solvent was completely dissolved in the aqueous phase. Then, the resultant solution was cooled in an ice bath and a cloudy solution was formed. Afterward, the mixture was centrifuged and the enriched deep eutectic solvent phase was analyzed by high‐performance liquid chromatography with ultraviolet detection for quantification of the analytes. The factors affecting the extraction efficiency were optimized. Under the optimized extraction conditions, the limits of detection for diazinon and fenitrothion were 0.3 and 0.15 μg/L, respectively. The calibration curves for diazinon and fenitrothion exhibited linearity in the concentration range of 1–100 and 0.5–100 μg/L, respectively. The relative standard deviations for five replicate measurements at 10.0 μg/L level of analytes were less than 2.8 and 4.5% for intra‐ and interday assays, respectively. The developed method was successfully applied to the determination of diazinon and fenitrothion in water and fruit juice samples.     

Magnetic micro‐solid‐phase extraction based on magnetite‐MCM‐41 with gas chromatography–mass spectrometry for the determination of antidepressant drugs in biological fluids

A new facile magnetic micro‐solid‐phase extraction coupled to gas chromatography and mass spectrometry detection was developed for the extraction and determination of selected antidepressant drugs in biological fluids using magnetite‐MCM‐41 as adsorbent. The synthesized sorbent was characterized by several spectroscopic techniques. The maximum extraction efficiency for extraction of 500 μg/L antidepressant drugs from aqueous solution was obtained with 15 mg of magnetite‐MCM‐41 at pH 12. The analyte was desorbed using 100 μL of acetonitrile prior to gas chromatography determination. This method was rapid in which the adsorption procedure was completed in 60 s. Under the optimized conditions using 15 mL of antidepressant drugs sample, the calibration curve showed good linearity in the range of 0.05–500 μg/L (r ² = 0.996–0.999). Good limits of detection (0.008–0.010 μg/L) were obtained for the analytes with good relative standard deviations of <8.0% (n  = 5) for the determination of 0.1, 5.0, and 500.0 μg/L of antidepressant drugs. This method was successfully applied to the determination of amitriptyline and chlorpromazine in plasma and urine samples. The recoveries of spiked plasma and urine samples were in the range of 86.1–115.4%. Results indicate that magnetite micro‐solid‐phase extraction with gas chromatography and mass spectrometry is a convenient, fast, and economical method for the extraction and determination of amitriptyline and chlorpromazine in biological samples.     

Pipette vial dispersive liquid–liquid microextraction combined with high‐performance liquid chromatography for the determination of benzoylurea insecticide in fruit juice

A simple, sensitive, and efficient method of using a pipette vial to perform dispersive liquid–liquid microextraction based on the solidification of floating organic droplets was coupled with high‐performance liquid chromatography (HPLC) and a diode array detector for the preconcentration and analysis of four benzoylurea insecticides in fruit juice. In this method, 1‐dodecanol was used as an extractant, and a snipped pipette was used as an experimental vial to simplify the procedure of collecting and separating solidified extractant. The experimental parameters were optimized using a Plackett–Burman design and one‐factor‐at‐a‐time method. Under the optimal conditions in the water model, the limits of detection for analytes varied from 0.03 to 0.28 μg/L, and the enrichment factors ranged from 147 to 206. Linearity was achieved for diflubenzuron and flufenoxuron in a range of 0.5–500 μg/L, for hexaflumuron in a range of 1–500 μg/L, and for triflumuron in a range of 5–500 μg/L. The correlation coefficients for the analytes ranged from 0.9986 to 0.9994 with recoveries of 91.4–110.9%. Finally, the developed technique was successfully applied to fruit juice samples with acceptable results. The relative standard deviations of the analytes at two spiking levels (50 and 200 μg/L) varied between 0.2 and 4.5%.     

Ionic liquid chemically bonded basalt fibers for in‐tube solid‐phase microextraction

Ionic liquids have been widely used in different fields by advantage of their specific properties. In this work, 1‐methyl‐3‐(3‐trimethoxysilyl propyl)imidazolium chloride was prepared and chemically bonded onto basalt fibers for in‐tube solid‐phase microextraction. Through combining in‐tube extraction device with high‐performance liquid chromatography equipped with a diode array detector, an online enrichment and analysis method for eight polycyclic aromatic hydrocarbons was established under the optimum conditions. A good enrichment factor (52–814), good linearity (0.10–15 and 0.20–15 μg/L), low limits of detection (0.03–0.05 μg/L), and low limits of quantitation (0.10–0.20 μg/L) were achieved using a sample volume of 50 mL. Analysis method was applied to the real samples including the groundwater and wastewater from a chemical industry park, some target analytes were detected and the relative recoveries were in the range of 80.4–116.8%.     

Loading controlled magnetic carbon dots for microwave‐assisted solid‐phase extraction: Preparation,extraction evaluation and applications in environmental aqueous samples

An adsorbent of carbon dot@poly(glycidyl methacrylate)@Fe₃O₄ nanoparticles has been developed for the microwave‐assisted magnetic solid‐phase extraction of polycyclic aromatic hydrocarbons in environmental aqueous samples prior to high‐performance liquid chromatography with UV/visible spectroscopy detection. Poly(glycidyl methacrylate) was synthesized by atom transfer radical polymerization. The chain length and amount of carbon dots attached on them can be easily controlled through changing polymerization conditions, which contributes to tunable extraction performance. The successful fabrication of the nano‐adsorbent was confirmed by transmission electronic microscopy, Fourier transform infrared spectroscopy, Raman spectroscopy and vibrating sample magnetometry. The extraction performance of the adsorbent was evaluated by using polycyclic aromatic hydrocarbons as model analytes. The key factors influencing the extraction, such as microwave power, adsorption time, desorption time and desorption solvents were investigated in detail. Under the optimal conditions, the microwave‐assisted method afforded magnetic solid‐phase extraction with short extraction time, wide dynamic linear range (0.02–200 μg/L), good linearity (R² ≥ 98.57%) and low detection limits (20–90 ng/L) for model analytes. The adsorbent was successfully applied for analyzing polycyclic aromatic hydrocarbons in environmental aqueous samples and the recoveries were in the range of 86.0–124.2%. Thus, the proposed method is a promising candidate for fast and reliable preconcentration of trace polycyclic aromatic hydrocarbons in real water samples.     

Fabrication of boron‐rich multiple monolithic fibers for the solid‐phase microextraction of carbamate pesticide residues in complex samples

To enrich carbamate pesticides from complex matrices, an adsorbent based on poly (vinylboronic anhydride pyridine complex‐co‐ethylenedimethacrylate) monolith was fabricated and utilized as the extraction phase of multiple monolithic fiber solid‐phase microextraction. Due to the abundant boron atoms in the monolith, the B–N coordination interaction between adsorbent and analytes play a key role in the efficient extraction of analytes. Under the optimized conditions, the monolithic fibers were combined with high‐performance liquid chromatography for the quantify trace levels of carbamate pesticides in environmental water and orange juice samples. For water sample, the limit of detection and limit of quantification were in the range of 0.017–0.29 and 0.057–0.96 μg/L, respectively. The related values in orange juice samples were 0.038–0.39 and 0.12–1.36 μg/kg, respectively. Besides, the proposed method also exhibits wide linearity, satisfactory coefficients of determination, and good precision. The introduced approach was successfully applied to determine trace target analytes in real‐life samples. The spiked recoveries with different fortified concentrations were in the range of 80.4–117% for water samples and 83.7–119% for fruit juice samples. The relative standard deviations were below 10%. The results evidence that the suggested method was convenient, reliable, and eco‐friendly for the monitoring of trace levels of carbamate pesticides in complex samples such as waters and juices.     

Silicon carbide nanomaterial as a coating for solid‐phase microextraction

Silicon carbide has excellent properties, such as corrosion resistance, high strength, oxidation resistance, high temperature, and so on. Based on these properties, silicon carbide was coated on stainless‐steel wire and used as a solid‐phase microextraction coating, and polycyclic aromatic hydrocarbons were employed as model analytes. Using gas chromatography, some important factors that affect the extraction efficiency were optimized one by one, and an analytical method was established. The analytical method showed wide linear ranges (0.1–30, 0.03–30, and 0.01–30 μg/L) with satisfactory correlation coefficients (0.9922–0.9966) and low detection limits (0.003–0.03 μg/L). To investigate the practical application of the method, rainwater and cigarette ash aqueous solution were collected as real samples for extraction and detection. The results indicate that silicon carbide has excellent application in the field of solid‐phase microextraction.     

Development of a solid‐phase microextraction fiber by the chemical binding of graphene oxide on a silver‐coated stainless‐steel wire with an ionic liquid as the crosslinking agent

Graphene oxide was bonded onto a silver‐coated stainless‐steel wire using an ionic liquid as the crosslinking agent by a layer‐by‐layer strategy. The novel solid‐phase microextraction fiber was characterized by scanning electron microscopy, energy‐dispersive X‐ray spectroscopy and Raman microscopy. A multilayer graphene oxide layer was closely coated onto the supporting substrate. The thickness of the coating was about 4 μm. Coupled with gas chromatography, the fiber was evaluated using five polycyclic aromatic hydrocarbons (fluorene, anthracene, fluoranthene, 1,2‐benzophenanthrene, and benzo(a)pyrene) as model analytes in direct‐immersion mode. The main conditions (extraction time, extraction temperature, ionic strength, and desorption time) were optimized by a factor‐by‐factor optimization. The as‐established method exhibited a wide linearity range (0.5–200 μg/L) and low limits of determination (0.05–0.10 μg/L). It was applied to analyze environmental water samples of rain and river water. Three kinds of the model analytes were quantified and the recoveries of samples spiked at 10 μg/L were in the range of 92.3–120 and 93.8–115%, respectively. The obtained results indicated the fiber was efficient for solid‐phase microextraction analysis.     

Mesoporous titanium oxide with high‐specific surface area as a coating for in‐tube solid‐phase microextraction combined with high‐performance liquid chromatography for the analysis of polycyclic aromatic hydrocarbons

Stainless‐steel wires coated with mesoporous titanium oxide were placed into a polyether ether ketone tube for in‐tube solid‐phase microextraction, and the coating sorbent was characterized by X‐ray diffraction and scanning electron microscopy. It was combined with high‐performance liquid chromatography to build an online system. Using eight polycyclic aromatic hydrocarbons as the analytes, some conditions including sample flow rate, sample volume, organic solvent content, and desorption time were investigated. Under optimum conditions, an online analysis method was established and provided good linearity (0.03–30 μg/L), low detection limits (0.01–0.10 μg/L), and high enrichment factors (77.6–678). The method was applied to determine target analytes in river water and water sample of coal ash, and the recoveries are in the range of 80.6–106.6 and 80.9–103.5%, respectively. Compared with estrogens and plasticizers, extraction coating shows better extraction efficiency for polycyclic aromatic hydrocarbons.     

Simultaneous analysis of 2‐methylimidazole, 4‐methylimidazole,and 5‐hydroxymethylfurfural potentially formed in fermented soy sauce by “quick,easy, cheap,effective, rugged,and safe” purification and UHPLC with tandem mass spectrometry

2‐Methylimidazole, 4‐methylimidazole and 5‐hydroxymethylfurfural are harmful by‐products potentially formed via Maillard reaction in fermented soy sauce. The present study proposed a new method based on “quick, easy, cheap, effective, rugged, and safe” purification and ultra high performance liquid chromatography with tandem mass spectrometry for the simultaneous analysis of 2‐methylimidazole, 4‐methylimidazole and 5‐hydroxymethylfurfural in fermented soy sauce. The sample was dissolved in water after addition of internal standard 4‐methylimidazole‐d₆ and extracted with acetonitrile. After dehydration, it was centrifuged and the supernatant was subsequently purified using two sorbents namely primary‐secondary amine and multi‐walled carbon nanotube. Three target analytes were separated by gradient elution and determined under multiple reactions monitoring mode. The limit of detection, matrix effect, recovery and precision of the developed method were investigated. Results found that three target analytes displayed excellent linearity in concentration range of 1–250 μg/L. Limit of detection was in the range of 0.3–1 μg/kg for three target analytes. The mean recoveries for fermented soy sauce samples at three spiked concentrations were in the range of 91.2–112.5%, and the intra‐ and interday precision were in the ranges of 3.6–9.2 and 7.1–10.8%, respectively. This validated method was successfully applied to determine 2‐methylimidazole, 4‐methylimidazole and 5‐hydroxymethylfurfural concentrations in fermented soy sauce.     

Application of micro‐solid‐phase extraction for the on‐site extraction of heterocyclic aromatic amines in seawater

An efficient on‐site extraction technique to determine carcinogenic heterocyclic aromatic amines in seawater has been reported. A micro‐solid‐phase extraction device placed inside a portable battery‐operated pump was used for the on‐site extraction of seawater samples. Before on‐site applications, parameters that influence the extraction efficiency (extraction time, type of sorbent materials, suitable desorption solvent, desorption time, and sample volume) were investigated and optimized in the laboratory. The developed method was then used for the on‐site sampling of heterocyclic aromatic amines determination in seawater samples close to distillation plant. Once the on‐site extraction completed, the small extraction device with the analytes was brought back to the laboratory for analysis using high‐performance liquid chromatography with fluorescence detection. Based on the optimized conditions, the calibration curves were linear over the concentration range of 0.05–20 μg/L with correlation coefficients up to 0.996. The limits of detection were 0.004–0.026 μg/L, and the reproducibility values were between 1.3 and 7.5%. To evaluate the extraction efficiency, a comparison was made with conventional solid‐phase extraction and it was applied to various fortified real seawater samples. The average relative recoveries obtained from the spiked seawater samples varied in the range 79.9–95.2%.     

Extraction and determination of trace amounts of three anticancer pharmaceuticals in urine by three‐phase hollow fiber liquid‐phase microextraction based on two immiscible organic solvents followed by high‐performance liquid chromatography

An automated three‐phase hollow fiber liquid‐phase microextraction based on two immiscible organic solvents followed by high‐performance liquid chromatography with UV–Vis detection method was applied for the extraction and determination of exemestane, letrozole, and paclitaxel in water and urine samples. n‐Dodecane was selected as the supported liquid membrane and its polarity was justified by trioctylphosphine oxide. Acetonitrile was used as an organic acceptor phase with desirable immiscibility having n‐dodecane. All the effective parameters of the microextraction procedure such as type of the organic acceptor phase, the supported liquid membrane composition, extraction time, pH of the donor phase, hollow fiber length, stirring rate, and ionic strength were evaluated and optimized separately by a one variable at‐a‐time method. Under the optimal conditions, the linear dynamic ranges were 1.8–200 (R² = 0.9991), 0.9–200 (R² = 0.9987) and 1.2–200 μg/L (R² = 0.9983), and the limits of detection were 0.6, 0.3, and 0.4 μg/L for exemestane, letrozole, and paclitaxel, respectively. To evaluate the capability of the proposed method in the analysis of biological samples, three different urinary samples were analyzed under the optimal conditions. The relative recoveries of the three pharmaceuticals were in the range of 91–107.3% for these three analytes.     

Application of sunflower stalk‐carbon nitride nanosheets as a green sorbent in the solid‐phase extraction of polycyclic aromatic hydrocarbons followed by high‐performance liquid chromatography

A green biocomposite of sunflower stalks and graphitic carbon nitride nanosheets has been applied as a solid‐phase extraction adsorbent for sample preparation of five polycyclic aromatic hydrocarbons in different solutions using high‐performance liquid chromatography with ultraviolet detection. Before the modification, sunflower stalks exhibited relatively low adsorption to the polycyclic aromatic hydrocarbons extraction. The modified sunflower stalks showed increased adsorption to the analytes extraction due to the increase in surface and existence of a π–π interaction between the analytes and graphitic carbon nitride nanosheets on the surface. Under the optimal conditions, the limits of detection and quantification for five polycyclic aromatic hydrocarbons compounds could reach 0.4–32 and 1.2–95 ng/L, respectively. The method accuracy was evaluated using recovery measurements in spiked real samples and good recoveries from 71 to 115% with relative standard deviations of <10% have been achieved. The developed method was successfully applied for polycyclic aromatic hydrocarbons determination in various samples—well water, tap water, soil, vegetable, and barbequed meat (kebab)—with analytes contents ranging from 0.065 to 13.3 μg/L. The prepared green composite as a new sorbent has some advantages including ease of preparation, low cost, and good reusability.     

Directly‐coupled‐column ultra‐fast liquid chromatography with diode array detection method for the rapid quantification of allergenic disperse dyes in water preconcentrated by magnetic solid‐phase extraction

A directly‐coupled‐column ultra‐fast liquid chromatography coupled with diode array detection method for the determination of 12 allergenic disperse dyes in river water at sub‐ppb levels has been developed and successfully validated. The analytical method is based on the use of two different reversed‐phased columns connected through a two‐position switching valve. A baseline separation was achieved by proper selection of stationary phases, mobile phases, and the use of a gradient elution in both dimensions. Furthermore, an easy‐to‐handle magnetic solid‐phase extraction procedure was developed for the preconcentration of 12 allergenic disperse dyes from river water. An enrichment factor of 100 times was obtained. The results showed excellent performance in terms of trueness (76.8–99.0%), precision (intraday: 2.2–8.0%, interday: 3.3–8.2%), and sensitivity (limits of determination, 0.027–1.46 μg/L). Twenty real samples collected from the outfalls in the Yaojiang, Yongjiang and Fenghuajiang estuary were analyzed, and three of the studied compounds were found in one collected sample (12.6 μg/L for disperse blue 7, 11.6 μg/L for disperse blue 106, and 0.22 μg/L for disperse blue 3).     

A bioinspired polydopamine approach toward the preparation of gold‐modified magnetic nanoparticles for the magnetic solid‐phase extraction of steroids in multiple samples

In this work, a simple, facile, and sensitive magnetic solid‐phase extraction method was developed for the extraction and enrichment of three representative steroid hormones before high‐performance liquid chromatography analysis. Gold‐modified Fe₃O₄ nanoparticles, as novel magnetic adsorbents, were prepared by a rapid and environmentally friendly procedure in which polydopamine served as the reductant as well as the stabilizer for the gold nanoparticles, thus successfully avoiding the use of some toxic reagents. To obtain maximum extraction efficiency, several significant factors affecting the preconcentration steps, including the amount of adsorbent, extraction time, pH of the sample solution, and the desorption conditions, were optimized, and the enrichment factors for three steroids were all higher than 90. The validity of the established method was evaluated and good analytical characteristics were obtained. A wide linearity range (0.8–500 μg/L for all the analytes) was attained with good correlation (R² ≥ 0.991). The low limits of detection were 0.20–0.25 μg/L, and the relative standard deviations ranged from 0.83 to 4.63%, demonstrating a good precision. The proposed method was also successfully applied to the extraction and analysis of steroids in urine, milk, and water samples with satisfactory results, which showed its reliability and feasibility in real sample analysis.     

Trace quantification of selected sulfonamides in aqueous media by implementation of a new dispersive solid‐phase extraction method using a nanomagnetic titanium dioxide graphene‐based sorbent and HPLC‐UV

Herein, a new dispersive solid‐phase extraction method using a nano magnetic titanium dioxide graphene‐based sorbent in conjunction with high‐performance liquid chromatography and ultraviolet detection was successfully developed. The method was proved to be simple, sensitive, and highly efficient for the trace quantification of sulfacetamide, sulfathiazole, sulfamethoxazole, and sulfadiazine in relatively large volume of aqueous media. Initially, the nano magnetic titanium dioxide graphene‐based sorbent was successfully synthesized and subsequently characterized by scanning electron microscopy and X‐ray diffraction. Then, the sorbent was used for the sorption and extraction of the selected sulfonamides mainly through π–π stacking hydrophobic interactions. Under the established conditions, the calibration curves were linear over the concentration range of 1–200 μg/L. The limit of quantification (precision of 20%, and accuracy of 80–120%) for the detection of each sulfonamide by the proposed method was 1.0 μg/L. To test the extraction efficiency, the method was applied to various fortified real water samples. The average relative recoveries obtained from the fortified samples varied between 90 and 108% with the relative standard deviations of 5.3–10.7%.     

Application of a dispersive solid‐phase extraction method using an amino‐based silica‐coated nanomagnetic sorbent for the trace quantification of chlorophenoxyacetic acids in water samples

Herein, an amino‐based silica‐coated nanomagnetic sorbent was applied for the effective extraction of two chlorophenoxyacetic acids (2‐methyl‐4‐chlorophenoxyacetic acid and 2,4‐dichlorophenoxyacetic acid) from various water samples. The sorbent was successfully synthesized and subsequently characterized by scanning electron microscopy, X‐ray diffraction, and Fourier‐transform infrared spectroscopy. The analytes were extracted by the sorbent mainly through ionic interactions. Once the extraction of analytes was completed, they were desorbed from the sorbent and detected by high‐performance liquid chromatography with ultraviolet detection. A number of factors affecting the extraction and desorption of the analytes were investigated in detail and the optimum conditions were established. Under the optimum conditions, the calibration curves were linear over the concentration range of 1–250, and based on a signal‐to‐noise ratio of 3, the method detection limits were determined to be 0.5 μg/L for both analytes. Additionally, a preconcentration factor of 314 was achieved for the analytes. The average relative recoveries obtained from the fortified water samples varied in the range of 91–108% with relative standard deviations of 2.9–8.3%. Finally, the method was determined to be robust and effective for environmental water analysis.     

Alternative solvent‐based methyl benzoate vortex‐assisted dispersive liquid–liquid microextraction for the high‐performance liquid chromatographic determination of benzimidazole fungicides in environmental water samples

Vortex‐assisted dispersive liquid–liquid microextraction using methyl benzoate as an alternative extraction solvent for extracting and preconcentrating three benzimidazole fungicides (i.e., carbendazim, thiabendazole, and fluberidazole) in environmental water samples before high‐performance liquid chromatographic analysis has been developed. The selected microextraction conditions were 250 μL of methyl benzoate containing 300 μL of ethanol, 1.0% w/v sodium acetate, and vortex agitation speed of 2100 rpm for 30 s. Under optimum conditions, preconcentration factors were 14.5–39.0 for the target fungicides. Limits of detection were obtained in the range of 0.01–0.05 μg/L. The proposed method was then applied to surface water samples and the recovery evaluations at three spiked concentration levels of 5, 30, and 50 μg/L were obtained in the range of 77.4–110.9% with the relative standard deviation <7.4%. The present method was simple, rapid, low cost, sensitive, environmentally friendly, and suitable for the trace analysis of the studied fungicides in environmental water samples.