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1.
One new and six known flavone glycosides were isolated from the MeOH extract of Melilotus neapolitana Ten. The new compound, identified as 7-O-beta-D-glucopyranosyloxy-4',5-dihydroxy-3-[O-alpha-L-rhamnopyranosyl-(1-->6)-3-O-beta-D-glucopyranosyloxy]flavone (1) by 1D and 2D NMR techniques and mass spectra, was isolated along with kaempferol-3-O-rutinoside (2), kaempferol-3-O-glucoside (3), rutin (4), quercetin-3-O-glucoside (5), isorhamnetin-3-O-rutinoside (6), and isorhamnetin-3-O-glucoside (7). The antioxidant and radical scavenging activities of these compounds and the whole crude methanol extract were evaluated. The organic extract can inhibit MDA marker's synthesis by 57%. All the metabolites displayed good reducing power, with the kaempferol (2,3) and isorhamnetin derivatives (6,7) being less active than the corresponding quercetin derivatives 4,5.  相似文献   

2.
周雯  杨开广  赵宝锋  张丽华  张玉奎 《色谱》2021,39(2):118-124
纳米载体一般是由天然高分子或人工合成高分子组成的、纳米级范畴的运输系统,具有减少药物毒性、提高药物的靶向性、增加药物有效性等优点。随着生物医学技术的进步,有研究表明,作为氧化代谢产物的活性氧(ROS)在疾病部位常常伴随着过表达的异常现象。基于此,近年来ROS刺激响应纳米载体获得了关注和发展,以不同响应机制的ROS响应基团为基础,发展了一系列的ROS响应纳米载体,实现了疾病部位ROS刺激下的药物特异性可控释放。该文聚焦于近年来常用于纳米载体的ROS响应基团,依据元素划分为两大类:硫族元素类响应基团(硫醚、缩硫酮、硒化物、二硒化物、碲化物)和其他元素类响应基团(芳香硼酸酯、过氧草酸酯、二茂铁);通过不同的设计理念将其引入纳米载体,根据ROS响应纳米载体的不同响应机制(疏水-亲水相变、断裂),探讨了载体各自的ROS响应情况、体外药物释放情况,以及在活体中的应用情况。  相似文献   

3.
Reactions of peroxyl radicals and peroxynitrite with o-vanillin (2-hydroxy 3-methoxy benzaldehyde), a positional isomer of the well-known dietary compound vanillin, were studied to understand the mechanisms of its free radical scavenging action. Trichloromethylperoxyl radicals (CCl3O 2 · ) were used as model peroxyl radicals and their reactions with o-vanillin were studied using nanosecond pulse radiolysis technique with absorption detection. The reaction produced a transient with a bimolecular rate constant of approx. 105 M−1s−1, having absorption in the 400–500 nm region with a maximum at 450 nm. This spectrum looked significantly different from that of phenoxyl radicals of o-vanillin produced by the one-electron oxidation by azide radicals. The spectra and decay kinetics suggest that peroxyl radical reacts with o-vanillin mainly by forming a radical adduct. Peroxynitrite reactions with o-vanillin at pH 6.8 were studied using a stopped-flow spectrophotometer. o-Vanillin reacts with peroxynitrite with a bimolecular rate constant of 3 × 103 M−1s−1. The reaction produced an intermediate having absorption in the wavelength region of 300–500 nm with a absorption maximum at 420 nm, that subsequently decayed in 20 s with a first-order decay constant of 0.09 s−1. The studies indicate that o-vanillin is a very efficient scavenger of peroxynitrite, but not a very good scavenger of peroxyl radical. The reactions take place through the aldehyde and the phenolic OH group and are significantly different from other phenolic compounds.  相似文献   

4.
Osteoclasts, together with osteoblasts, control the amount of bone tissue and regulate bone remodeling. Osteoclast differentiation is an important factor related to the pathogenesis of bone-loss related diseases. Reactive oxygen species (ROS) acts as a signal mediator in osteoclast differentiation. Simvastatin, which inhibits 3-hydroxy-3-methylglutaryl coenzyme A, is a hypolipidemic drug which is known to affect bone metabolism and suppresses osteoclastogenesis induced by receptor activator of nuclear factor-κB ligand (RANKL). In this study, we analyzed whether simvastatin can inhibit RANKL-induced osteoclastogenesis through suppression of the subsequently formed ROS and investigated whether simvastatin can inhibit H2O2-induced signaling pathways in osteoclast differentiation. We found that simvastatin decreased expression of tartrate-resistant acid phosphatase (TRAP), a genetic marker of osteoclast differentiation, and inhibited intracellular ROS generation in RAW 264.7 cell lines. ROS generation activated NF-κB, protein kinases B (AKT), mitogen-activated protein kinases signaling pathways such as c-JUN N-terminal kinases, p38 MAP kinases as well as extracellular signal- regulated kinase. Simvastatin was found to suppress these H2O2-induced signaling pathways in osteoclastogenesis. Together, these results indicate that simvastatin acts as an osteoclastogenesis inhibitor through suppression of ROS-mediated signaling pathways. This indicates that simvastatin has potential usefulness for osteoporosis and pathological bone resorption.  相似文献   

5.
A new cyano-substituted diarylethene derivative (R-NH2) with reversible far-red mechanofluorochromic property was synthesized and confirmed by standard spectroscopic methods. To the best of our knowledge, the 684 nm red-shifted wavelength of the ground R-NH2 is the longest wavelength that has appeared in the literature. The mechanofluorochromic mechanism was investigated by small and wide-angle X-ray scattering and was ascribed to the destruction of crystalline structure. More in-depth study by infrared spectra and time-resolved emission-decay behaviors showed that the changes of C–H out-of-plane bending vibrations in aryl group of the compound and the obvious increase of fluorescence lifetime might be the fundamental reasons. The synthetic strategy reported here can be extended to prepare more and more long-wavelength emission mechanofluorochromic materials, which can broaden the scope of application of such materials and for thoroughly understanding the mechanofluorochromic mechanism.  相似文献   

6.
采用松木热解生物炭为模板构筑Fe-Ni复合载氧体(Fe-Ni/BC),并与溶胶-凝胶法制备的NiFe2O4载氧体(NiFe2O4/SG)对比,采用SEM、XRD、XPS、BET、H2-TPR、TG-redox循环等表征方法考察载氧体的物理、化学性质,并在固定床上进行化学链制氢循环实验。结果表明,Fe-Ni/BC载氧体为Ni0.6Fe2.4O4与Fe2O3的混合晶体,保留了生物炭骨架并形成了大孔结构。与NiFe2O4/SG相比,Fe-Ni/BC平均粒径更小,比表面积更大,吸附氧含量更高,更有利于氧的释放。在固定床实验过程中,Fe-Ni/BC表现出更强的化学链制氢与抗积炭性能,其最大产氢速率是NiFe2O4/SG的1.58倍,制取H2的相对浓度可达到99.5%以上。  相似文献   

7.
8.
The copper complexes [Cu(Pyimpy)(H2O)](ClO4)2 (1), [Cu(Pyimpy)2](ClO4)2 (2), [Cu(Pyimpy)(Cl)2]·2H2O (3·2H2O), [Cu(Pyimpy)(N3)(ClO4)]2 (4) and [Cu(Pyimpy)(SCN)(ClO4)]2 (5) were synthesized and characterized by spectroscopic techniques, crystal structures and electrochemical studies (Pyimpy: (2-((2-phenyl-2-(pyridin-2-l)hydrazono)methyl)pyridine)). The superoxide scavenging activity of the two water soluble complexes 1 and 3 was examined. DNA interaction studies by UV-Vis absorption spectral changes during a titration experiment indicated the generation of new species. These small molecule SOD mimics exhibited excellent DNA cleavage activity in the presence of H2O2 as well as 2-mercaptoethanol. Complexes 1-5 exhibited better cytotoxicity compared to CuCl2·2H2O and the ligand Pyimpy, and showed more potency than cisplatin for MCF-7, PC-3 and HEK-293 cells. Complex 3 exhibited the highest potency for MCF-7, PC-3 and HEK-293 cells compared to the other complexes.  相似文献   

9.
Biologically derived value-added products from coal   总被引:7,自引:0,他引:7  
The Idaho National Engineering Laboratory (INEL) has for several years conducted research in the area of coal bioprocessing. Fundamental and exploratory research has been directed toward the conversion of coal to cleaner fuels and the remediation of harmful byproducts of coal utilization. Specific research projects have involved coal depolymerization, coal solubilization, removal of organosulfur and pyritic sulfur from coal, the molecular biology of coal-modifying microorganisms, removal of ash-forming minerals from coal, conversion of coal combustion gases, and the development of novel coal bioreactors. Notable research accomplishments include elucidation of mechanisms by which microorganisms solubilize different portions of coal, discovery and characterization of microorganisms capable of depolymerizing macromolecular coal, discovery of microorganisms capable of removing organosulfur from coal, development of technologies critical to analyzing biologically mediated depolymerization and organosulfur removal, novel methods of pyritic sulfur removal, novel methods of removal (and conversion) of CO2, SOx, and NOx from combustion gases, and new technologies for bioconversion of waste gypsum generated in coal combustion gas scrubbers.  相似文献   

10.
A novel water-soluble cystine C_(60)derivative was synthesized in the presence of the catalyst,tetrabutylammonium hydroxide (TBAH).The product was characterized by FT-IR,UV,~1H NMR,~(13)C NMR,MS and elemental analysis.Furthermore,the scavenging ability to superoxygen anion radical O_2~(·-)and hydroxyl radical ~·OH was studied by chemiluminescence.It was found that cystine C_(60)derivative showed an excellent efficiency in eliminating superoxygen anion radical and hydroxyl radical.The 50% inhibition concentration(IC_(50))for superoxygen anion radical and hydroxyl radical were 0.167 and 0.008 mg/mL,respectively.  相似文献   

11.
Acylnitroso dienophile 3 derived from D-bornane-10,2-sultam undergoes cycloaddition with high yields and complete facial selectivity to cyclopentadiene and cyclohexadiene. When the nitroso group is attached to the sultam nitrogen, it is no longer reactive in Diels-Alder reactions.  相似文献   

12.
Four phenanthrene and one aporphine alkaloids semi-synthesized from boldine were evaluated for their inhibitory effect on reactive oxygen species (ROS) generation. ROS generation by neutrophils stimulated with N-formyl-methionyl-leucyl-phenylalanine was inhibited in a concentration dependent manner. Alkaloids exerted similar inhibitory effect in the hypoxanthine-xanthine oxidase system than in stimulated neutrophils, which could be attributed to a direct ROS scavenging activity. None of the alkaloids assayed had any effect on xanthine oxidase activity. Therefore the synthesized alkaloids might constitute an alternative therapy in inflammation disorders in which ROS generation is involved.  相似文献   

13.
We report the development of a new fluorescence sensor for reactive oxygen species (ROS) based on a benzofurazan structure. The sensor, NBFhd, is initially non-fluorescent and reacts with peroxyl radicals by hydrogen transfer in an aqueous medium under neutral conditions to release the fluorescent N-methyl-4-amino-7-nitrobenzofurazan (NBF) and 1,4-benzoquinone. NBFhd shows excellent contrast and no interference in the region of cell autofluorescence and is a new tool to detect ROS in homogeneous and biological systems.  相似文献   

14.
Reactive blends prepared from methoxysilane terminated silicone polymers and silylated soybean oil are described and characterized. Although simple mixing of soy and silicones results in gross phase separation, homogeneous polymeric products are obtained by introducing reactive sites. These products can be used as protective coatings, additives to adhesives and new sealants. Exposure of the mixtures to moisture leads to hydrolysis of the methoxysilanes and subsequent condensation of the resulting silanols that yields stable siloxane linkages between the two immiscible phases. FTIR, TGA, and swell‐gel analyses indicate effective formation of these siloxane crosslinks. Reactive blends containing less than 20% silylated oil appeared completely transparent but increasing the soy content decreased the optical transparency. SEM micrographs reveal the silicone polymer as the continuous phase with individual spherical silylated soy oil particles distributed in it. The properties of these reactive blends vary from high elongation elastomers to high modulus resins depending on the composition. © 2016 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2016 , 54, 3086–3093  相似文献   

15.
Recently, reactive oxygen species (ROS) have been studied as a regulator of differentiation into specific cell types in embryonic stem cells (ESCs). However, ROS role in human ESCs (hESCs) is unknown because mouse ESCs have been used mainly for most studies. Herein we suggest that ROS generation may play a critical role in differentiation of hESCs; ROS enhances differentiation of hESCs into bi-potent mesendodermal cell lineage via ROS-involved signaling pathways. In ROS-inducing conditions, expression of pluripotency markers (Oct4, Tra 1-60, Nanog, and Sox2) of hESCs was decreased, while expression of mesodermal and endodermal markers was increased. Moreover, these differentiation events of hESCs in ROS-inducing conditions were decreased by free radical scavenger treatment. hESC-derived embryoid bodies (EBs) also showed similar differentiation patterns by ROS induction. In ROS-related signaling pathway, some of the MAPKs family members in hESCs were also affected by ROS induction. p38 MAPK and AKT (protein kinases B, PKB) were inactivated significantly by buthionine sulfoximine (BSO) treatment. JNK and ERK phosphorylation levels were increased at early time of BSO treatment but not at late time point. Moreover, MAPKs family-specific inhibitors could prevent the mesendodermal differentiation of hESCs by ROS induction. Our results demonstrate that stemness and differentiation of hESCs can be regulated by environmental factors such as ROS.  相似文献   

16.
The molybdenum nitrido complex 15NMo[N(R)Ar]3 (where R = C(CD3)2CH3, Ar = 3,5-C6H3Me2) reacted with the anhydride of trifluoroacetic acid at room temperature to afford the correspondent organonitrogen compound in almost quantitative yield without the necessity of using additional reagents to achieve the C-N coupling.  相似文献   

17.
Phytochemical investigation of Ajuga bracteosa Wall ex Benth. (Labiatae) resulted in the isolation of a new phenolic compound, ajuganane (1) and three known compounds, 3,4'-dihydroxy-3,6,7-trimethoxyflavone, 7-hydroxy-3,6,3',4'-tetramethoxyflavone and ursolic acid. The structure of the new compound was elucidated by detailed spectroscopic (1H, 13C NMR, COSY, HMQC, HMBC), and HR-EI-MS analysis.  相似文献   

18.
Nitric oxide donor SNAP induced apoptosis in primary rat cerebral cortical neurons, which was characterized morphologically by chromatin condensation and the formation of apoptotic bodies. With redox-sensitive fluorescence probes DCFH-DA and DHR123, the formation of endogenous reactive oxygen species (ROS) inside cells during the apoptosis process was monitored by laser confocal scanning microscopy (LCSM). SNAP treatment also caused the accumulation of extracellular hydrogen peroxide. Pretreatment with the nitric oxide scavenger hemoglobin could effectively inhibit the formation of endogenous ROS and protect neurons from apoptosis. The results suggested that ROS might be involved in NO-induced apoptosis in neuronal cells.  相似文献   

19.
Kim SH  Sung SH  Choi SY  Chung YK  Kim J  Kim YC 《Organic letters》2005,7(15):3275-3277
[structure: see text]. Idesolide, a new spiro compound possessing tetrahydrobenzodioxole structure, was isolated from the fruits of Idesia polycarpa Maxim. Its structure was established by NMR and MS spectroscopic analysis with single-crystal X-ray experiments. Idesolide significantly reduced nitric oxide production induced by lipopolysaccharide in BV2 microglial cells.  相似文献   

20.
The fluorescent protein aptly named "Killer Red" (KRed) is capable of killing transfected cells and inactivating fused proteins upon exposure to visible light in the presence of oxygen. We have investigated the source of the bioactive species through a variety of photophysical and photochemical techniques. Our results indicate a Type I (electron transfer mediated) photosensitizing mechanism.  相似文献   

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