用于卵母细胞玻璃化冷冻的几种方法的传热分析

使用高冷冻速率和低浓度冷冻保护液来实现卵母细胞的玻璃化冷冻保存是目前卵母细胞低温保存的主要发展方向.文中选择四种具有代表性的用于卵母细胞玻璃化冷冻的方法:弹射法、OPS法、QMC法和Cryotop法,通过对它们传热特点的分析以及动态温度场分布的模拟计算,找出冷冻速率最高的方法.结果表明,Cryotop法的传热性能优于其...     

Vitrification of human spermatozoa without cryoprotectants

The aim of our study was to investigate the effects of vitrification (cooling rate approximately 10000(C/min) without cryoprotectants on swim-up prepared human spermatozoa in comparison to standard conventional freezing with cryoprotectants. Motility, morphology, rate of viability and acrosome reaction of spermatozoa were evaluated. The described method of cryopreservation of human spermatozoa by direct plunging into liquid nitrogen slush without cryoprotectants was effective and could be recommended for routine IVF.     

Aseptic minimum volume vitrification technique for porcine parthenogenetically activated blastocyst

Minimum volume vitrification may provide extremely high cooling and warming rates if the sample and the surrounding medium contacts directly with the respective liquid nitrogen and warming medium. However, this direct contact may result in microbial contamination. In this work, an earlier aseptic technique was applied for minimum volume vitrification. After equilibration, samples were loaded on a plastic film, immersed rapidly into factory derived, filter-sterilized liquid nitrogen, and sealed into sterile, pre-cooled straws. At warming, the straw was cut, the filmstrip was immersed into a 39 degree C warming medium, and the sample was stepwise rehydrated. Cryosurvival rates of porcine blastocysts produced by parthenogenetical activation did not differ from control, vitrified blastocysts with Cryotop. This approach can be used for minimum volume vitrification methods and may be suitable to overcome the biological dangers and legal restrictions that hamper the application of open vitrification techniques.     

Numerical analysis to determine the performance of different oocyte vitrification devices for cryopreservation

Vitrification is widely used for cryopreservation of oocytes. The present study has built the theoretical models of four vitrification systems (micro-droplet, open pulled straws, quartz micro-capillary and cryotop), and performed numerical analysis to predict the cooling rates. The numerical analysis shows that the average cooling rate of the cryotop system was higher than those of other three systems between 298K and 100K. In addition, the effects of other process parameters on the cooling rate with the cryotop system were also investigated, including the thickness of the carrier, the volume of cryoprotectant agent, the temperature of cold source as well as the heat transfer coefficient, when plunging into liquid nitrogen.     

浆氢与浆氮技术研究现状

浆态是一种固态与液态物质共存的状态,即在液体中含有固体小颗粒。浆氢在用作火箭和航天飞机的燃料方面有很广泛的应用前景;采用浆氮作为高温超导电缆的制冷剂则会明显提高电缆的冷却性能。主要介绍了浆氢和浆氮的制备方法,密度和流量的测量技术,及其管内流动及换热特性。     

液氮流动沸腾换热研究综述

作为一种优良的低温冷却剂,液氮在航天、电子工业、超导磁体和超导电缆冷却及低温生物医疗等领域应用广泛。在这些应用中,准确预测液氮的流动沸腾换热特性对于系统设计和安全运行十分重要。该文主要介绍了液氮流动沸腾换热的特点,对已有的实验和数值模拟结果进行了归纳;并比较了四个液氮流动沸腾换热计算关联式的预测情况,以及关联式中干度、质量流量、热流密度和压力对换热系数的影响;指出了有待进一步研究的课题。     

R600a喷雾冷却系统换热过程

以R600a压力式封闭系统喷雾冷却过程为研究对象,对其换热过程进行分析。对液滴撞击热面后的状态进行建模,分析了其运动状态。通过忽略液膜的对流换热,引入韦伯数来简化并修正雾滴与热源表面的对流换热系数关联式;借鉴二次成核理论,通过单位时间内,单位面积上覆盖的雾滴数量对核态沸腾换热系数关联式修正。通过上述分析,以对流换热和核态沸腾换热两种机理为中心,建立了新的换热系数关联式。通过与其他文献的关联式、实验测量值进行比较、不同工质进行比较、不同实验系统比较,发现该式预测值和实验测量值偏差在±20%以内,能够很好地预测压力式封闭系统喷雾冷却过程的换热系数。     

低温预冷换热规律及表面改性影响研究

调研了低温管路预冷及两相换热的研究现状,阐述了低温预冷瞬态换热特性;介绍了金属表面改性对预冷规律的影响,仿真了低导热涂层管的预冷换热规律.研究发现:液氮预冷主要由膜态沸腾支配,且预冷耗时较长;液氢预冷未见膜态沸腾,预冷耗时更短.促进膜态沸腾向过渡沸腾的更早转化有利于预冷加速,且转化温度迁移可通过内壁表面结构改性实现,可...     

喷雾冷却相变传热的建模与模拟计算

喷雾冷却是一种高效的热控技术,为了探索形成完善的喷雾冷却技术设计流程,文章开展了喷雾冷却传热过程的建模研究.针对喷雾冷却传热过程的模拟计算,基于喷雾冷却相变传热的4个传热机制:液膜对流传热、池沸腾传热、二次气泡沸腾传热、二次气泡高频化机制,利用Monte Carlo方法描述了不同粒径与速率分布的液滴撞击液膜并生成二次气...     

Simplified technique of human ovarian tissue freezing: quick cooling from -36 degree C

Standard protocol of freezing of human ovarian tissue presupposes the very slow cooling (-0.3 C/min) from auto-seeding to -40 C, then slow cooling (-10 C/min) to -140 C and then direct plunging into liquid nitrogen. The aim of this investigation was to compare the -10 C/min cooling rate of human ovarian tissue from -40 C to -140 C with the -220 C/min cooling rate (direct plunging into liquid nitrogen) from -36 degree C. After post-thawing in vitro culture of tissue, hormonal activity as well as follicle viability was evaluated. After culture of fresh tissue pieces (Group 1), pieces after freezing and thawing with slow cooling (-10 C/min) from -40 C (Group 2) and pieces after freezing and thawing with direct plunging into liquid nitrogen (-220 C/min) from -36 C (Group 3), the supernatants showed estradiol 17-ss concentrations of 481, 441 and 459 pg per ml, respectively, and progesterone concentrations of 9.05, 5.06, 4.87 ng per ml, respectively. It is concluded that 94, 96, and 98 percent follicles for Groups 1, 2 and 3, respectively, were normal. Technique of human ovarian tissue cryopreservation with very slow cooling to -36 C and then direct plunging into liquid nitrogen with -220 C/min cooling rate is tolerated without apparent detriment.     

窄缝通道中液氮的沸腾传热研究第一部分:窄缝通道尺寸对传热特性的影响

窄缝通道中的液氮沸腾传热特性有别于开放空间中的情况.文中对常压下液氮在矩形窄缝通道内的沸腾传热进行了实验研究,系统地研究了窄缝几何结构参数和倾角对液氮的沸腾传热特性的影响.     

液氮中导线加热丝的沸腾传热特性研究

本文以50μm的磷青铜丝作为加热丝和测温元件,采用控制热流密度的方式测量了0°-90°倾角下加热丝在液氮中的沸腾曲线,结果表明:核态沸腾在增加热流密度时存在滞后现象;Bromley公式能准确的预测出膜态沸腾换热曲线的斜率,Zuber模型和Kutateladze公式预测水平细加热丝的CHF误差在15%以内;对于Leidenfrost热流密度的预测,常温流体的计算模型并不适用;CHF随倾角的变化较大,且大于加热平面在相同倾角下的变化幅度。     

微液膜动力学特性与稳定性实验研究

气-液两相流设备的性能受限于临界热流密度,开展流动微液膜动力学特性及其稳定性的相关研究是深入理解沸腾危机及临界热流密度机理的关键。采用光学玻璃制成的矩形通道作为实验段,使用微流量齿轮泵驱动去离子水,使其在实验通道入口处与在其上部流动的压缩空气接触形成同向流动的分层流。利用共轭光学探测器对流动微液膜的厚度进行了测量,利用高速摄像机对气-液两相分层流波动特性进行了可视化观测。研究表明,在绝热情况下,当液速一定时,液膜的平均厚度随着气速增加而减小,当气速增加到某一阈值时会导致液膜破裂。     

Cryopreservation of garlic (allium sativum L.) using plant vitrification solution 2

Most cryopreservation procedures are optimized using a small number of germplasm accessions. We classified the garlic (Allium sativum L.) accessions that were selected for our studies based on genotype as identified using amplified fragment length polymorphism markers. Although recovery was variable, shoots regenerated from a broad range of the accessions after cryo-exposure. Garlic shoot tips were excised from cloves, surface sterilized, and placed on media at 5 degree C for 2 days prior to cryopreservation. Shoot tips were then treated with sucrose-glycerol for 20 minutes, plant vitrification solution 2 (PVS2; 15 percent w/v ethylene glycol, 15 percent w/v DMSO, 30 percent w/v glycerol, 13.7 percent w/v sucrose) at 0 degree C, and then plunged on foils into liquid nitrogen slush. Explants were recovered in 1.2 M sucrose for 20 minutes and then plated onto Gamborgs B5 medium containing alpha-naphthaleneacetic acid (NAA) and 6-(gammagamma-dimethylallylamino purine) (2-iP). Our results demonstrate that genotypically diverse accessions of garlic can be successfully cryopreserved.     

Cryopreservation of human embryonic stem cells: a protocol by programmed cooling

Human embryonic stem (ES) cells have far-reaching applications in the areas of tissue engineering, regenerative medicine, pharmacology and basic scientific research. Although the culture conditions can maintain the human ES cells in an undifferentiated state for a transient period, spontaneous differentiation has also been observed during the routine culturing of ES cells. However, the maintenance of ES cells in the undifferentiated, pluripotent state for extended periods of time will be required in many areas of scientific research. Cryopreservation is a technology with potentially far reaching implication for the development and widespread use of such cell lines. This study was undertaken to develop and optimize a protocol for cryopreservation of human ES cells through programmed cooling. The effects of the seeding temperature, the cooling rate and the sub-zero temperature to which the samples were cooled before plunging into liquid nitrogen(the terminal temperature), all significantly affected the recovery of cryopreserved ES cells. After studying these factors, an improved protocol was obtained: the sample was cooled from 0 degree C to -35 degree C at a cooling rate of 0.5 degree per min, with seeding was set at -10 degree C, before being plunged immediately into the liquid nitrogen. Using this protocol, 9 of 11 colony fragments survived freezing and thawing and could be cultured for prolonged periods. They retained the properties of pluripotent cells, had a normal karyotype and showed histochemical staining for alkaline phosphatase.     

低温流体在多孔表面的池沸腾研究综述

低温流体尤其是液氮在航天、电子冷却、低温生物医疗与超导磁体与电缆等领域有着广泛的应用.文中对光滑与多孔表面上的流体核态沸腾换热与临界热流密度的研究进行了归纳；总结了低温流体池沸腾的研究现状；比较了低温流体与常见制冷剂以及水在物性上的主要差异；综合分析了加热表面材料、多孔层厚度、孔隙率、烧结颗粒直径、平均孔隙直径与压力等...     

Cryopreservation of seeds of a Thai orchid (Doritis pulcherrima lindl. ) by vitrification

Seeds from selfing of a Thai orchid (Doritis pulcherrima Lindl.) were successfully cryopreserved in liquid nitrogen (LN) using the vitrification method. Seeds from 3-month-old pods were sufficiently dehydrated in 2 ml cryotubes filled with highly concentrated vitrification solution (PVS2) at 25 +/- 2 degree C for 50 min. The seeds were then rapidly plunged into LN. After rapid warming, the PVS2 solution was replaced with 0.5 ml of 1.2M sucrose in modified Vacin and Went (1949) (VW) solution and kept at 25 +/- 2 degree C for 20 min prior to transfer on VW agar medium. About 62% of cryopreserved seeds treated with PVS2 solution were able to develop into normal seedlings while without that treatment there was no survival. This vitrification protocol appears to be a promising technique for the cryopreservation of some Thai orchid germplasm     

Cryopreservation of garlic bulbil primordia by the droplet-vitrification procedure

The droplet-vitrification protocol, a combination of droplet-freezing and solution-based vitrification was applied for cryopreserving garlic bulbil primordia. The highest survival and regeneration percentages of cryopreserved primordia (90.1 to 95.0 percent and 82.7 to 85.0 percent, respectively) were achieved after preculture for 2-4 days at 10 degree C on solid medium with 0.1 - 0.3 M sucrose, loading for 50 minutes in liquid medium with 2 M glycerol + 0.5 M sucrose, dehydration with PVS3 vitrification solution for 90-150 min, cooling primordia in 5 microl droplets of PVS3 vitrification solution placed on aluminum foil strips by dipping these strips in liquid nitrogen, warming them by plunging the foil strips into pre-heated (40 degree C) 0.8 M sucrose solution for 30 s and further incubation in the same solution for 30 minutes. The optimized droplet-vitrification protocol was successfully applied to bulbil primordia of five garlic varieties originating from various countries and to immature bulbils of two vegetatively propagated Allium species, with regeneration percentages ranging between 77.4 - 95.4 percent.     

Microscopic Boiling of Liquid Nitrogen Induced by a Pulsed Laser Irradiation

An experimental investigation was conducted to explore the characteristics of microscopic boiling induced by firing a microsecond pulsed laser beam on a thin platinum (Pt) film that immerged in the liquid nitrogen (LN₂) cryostat. High-speed photography aided by a high-voltage lighting system was employed to visually observe the bubble formation and the dynamical boiling process of LN₂. A rapid transient temperature-measuring system was designed to record the temperature evolution of the heating surface. Explosive boiling, characterized by bubble cluster, was observed within LN₂ at the early stage of laser heating, and conventional boiling followed after a certain time. The transition time, therefore, was introduced for separating these two different boiling modes. The temperature of Pt film rose sharply to its maximum during laser pulse, with a very high rising rate of about 10⁷ K/s, and then dropped rapidly after laser irradiation. A model of bubble cluster was proposed to describe the explosive boiling heat transfer, and the latent heat released by bubble collapse in explosive boiling was explored as an important mechanism considerably influencing the boiling heat transfer.     

Comparative Study of Wetting and Cooling Performance of Polymer–Salt Hybrid Quench Medium With Conventional Quench Media

Wetting kinetics, kinematics, and cooling performance of a polymer–salt hybrid quenchant were investigated. The rewetting phenomenon for brine, water, polymer, and polymer–salt hybrid solutions was characterized as rapid uniform, fast non-uniform, slow uniform, and fast uniform processes, respectively. A dimensionless rewetting time was proposed to assess the nature of the wetting front. The hybrid quenchant showed higher heat transfer during vapor and transition boiling and lower heat transfer during nucleate boiling and convective cooling. The presence of salt in the hybrid solution resulted in early destabilization of the vapor film and an increase in wetting front velocity and rewetting temperature. The polymer constituent delayed the rewetting phenomenon.