一种基于全息术的光学系统闭环像差补偿方法

提出了一种基于21单元变形镜与全息波前传感器的全息自适应光学系统, 并对其像差校正能力进行了分析. 首先描述了全息波前传感器基本原理, 并在薄全息图近似下给出基于快速傅里叶变换算法的全息波前传感器数值模型; 然后基于21单元变形镜的数值模型, 分析了该变形镜的波前校正能力; 在此基础上, 数值模拟并实验验证了全息自适应光学系统对静态像差的闭环校正能力.     

数字全息显微术中重建物场波前的相位校正

数字全息显微术克服了传统光学显微术无法直接提取样品相位信息的缺点,可以对活体细胞组织等相位型生物样品进行定量测量和有效观察。但在数字全息显微成像过程中,像场弯曲会对再现像相位分布的测量和观察产生影响。提出一种采用相位相减来校正数字全息再现像像场弯曲的方法。通过在样品加入前后两次拍摄全息图,并对数值重建像分别进行去包裹运算再令其相减,即可实现对像场弯曲的有效校正,对蝉翼和大蒜表皮细胞等相位型物体进行测量,并采用数值校正和相位相减两种方法对像场弯曲进行校正。与现有的数值校正方法相比,利用相位相减获得样品三维相位信息的方法更为简单、可靠,是校正像数字全息再现场弯曲的有效方法。     

优化的数字全息显微成像系统

基于预放大数字全息显微系统的全息图记录与再现过程及其点扩散函数的分析, 从成像分辨率、成像质量及实现的难易程度等方面对数字全息显微中六种常见记录光路系统的成像性能进行了对比研究. 结果表明, 像面数字全息术具有最高的成像分辨率及成像质量, 其成像分辨率与记录器件的光敏面尺寸无关, 该系统对信息的记录是完整的, 而且记录过程不必考虑物体被照亮区域的大小, 再现过程非常简单, 是优化的数字全息显微成像系统. 等波面弯曲的物参光像面数字全息术非常有利于位相解包裹及位相畸变补偿的正确进行, 该系统更适合于位相显微. 实验结果验证了理论分析的正确性. 关键词： 显微数字全息 预放大数字全息 像面数字全息 分辨率     

显微数字全息中的3维信息重建

通过理论分析和模拟验证,研究了基于横向剪切的数字全息相位重建方法,分析并指明了详细的重建过程,提出了利用平坦区域相位数据进行线性拟合,从而获得线性相位畸变系数的方法,并指出对原始包裹相位图进行1维相位展开是横向剪切法重建数字全息相位信息的前提。对无噪声及含有噪声的全息图进行了数值重建,结果表明:对于弱噪声干扰的全息图,该方法很有效;而对于较强噪声干扰的全息图,采用中值滤波方法对原始相位图进行滤波后再重建,并对重建的相位图再次进行中值滤波,可以得到高质量的再现像;减小再现像平面抽样间隔,使剪切相位图中相邻的两个像元之间相位差的最大值小于2π,才可以获得正确的相位重建。     

A modified phase diversity diffraction wavefront sensor with a grating

The phase diversity wavefront sensor is one of the tools used to estimate wavefront aberration, and it is often used as a wavefront sensor in adaptive optics systems. However, the performance of the traditional phase diversity wavefront sensor is limited by the accuracy and dynamic ranges of the intensity distribution at the focus and defocus positions of the CCD camera. In this paper, a modified phase diversity wavefront sensor based on a diffraction grating is proposed to improve the ability to measure the wavefront aberration with larger amplitude and higher spatial frequency. The basic principle and the optics construction of the proposed method are also described in detail. The noise propagation property of the proposed method is also analysed by using the numerical simulation method, and comparison between the diffraction grating phase diversity wavefront sensor and the traditional phase diversity wavefront sensor is also made. The simulation results show that the diffraction grating phase diversity wavefront sensor can obviously improve the ability to measure the wavefront aberration, especially the wavefront aberration with larger amplitude and higher spatial frequency.     

Digital self-referencing quantitative phase microscopy by wavefront folding in holographic image reconstruction

A completely numerical method, named digital self-referencing holography, is described to easily accomplish a quantitative phase microscopy for microfluidic devices by a digital holographic microscope. The approach works through an appropriate numerical manipulation of the retrieved complex wavefront. The self-referencing is obtained by folding the retrieved wavefront in the image plane. The folding operation allows us to obtain the correct phase map by subtracting from the complex region of interest a flat area outside the microfluidic channel. To demonstrate the effectiveness of the method, quantitative phase maps of bovine spermatozoa and in vitro cells are retrieved.     

A modified phase diversity wavefront sensor with a diffraction grating

Phase diversity wavefront sensor is one of the useful tools to estimate the wavefront aberration, and it is often used as a wavefront sensor in adaptive optics system. However, the performance of the traditional phase diversity wavefront sensor is limited by the accuracy and dynamic ranges of the intensity distribution at focus and defocus positions of the CCD camera. In this paper, a modified phase diversity wavefront sensor based on a diffraction grating is proposed to improve the ability to measure the wavefront aberration with larger amplitude and higher spatial frequency. The basic principle and the optics construction of the proposed method are also described in detail. The noise propagation property of the proposed method is also analysed by using the numerical simulation method, and comparison between the diffraction grating phase diversity wavefront sensor and the traditional phase diversity wavefront sensor is also made. The simulation results show that the diffraction grating phase diversity wavefront sensor can obviously improve the ability to measure the wavefront aberration, especially the wavefront aberration with larger amplitude and higher spatial frequency.     

Analysis and demonstration of multiplexed phase computer-generated hologram for modal wavefront sensing

We propose to implement a modal wavefront sensor (MWS) using a multiplexed phase computer-generated hologram (MPCGH). Based on general orthogonal aberration modes, the theoretical treatments of the MWS employing a MPCGH are presented with scalar diffraction approximations and Fourier analysis. Under the small aberration approximations, we give the analytical formula for characterizing the relationship between the sensor signal and the amplitude of the aberration mode. We design several MPCGHs with an effective method of modified off-axis reference beam holograms superposition, and code some common orthogonal Zernike aberration modes into the MPCGH. The numerical simulation is carried out to investigate the performance of MWS to detect particular aberration mode(s). The results exhibit the expected responses of the corresponding symmetric spot pair, and indicate that the wavefront distorted by a special Zernike aberration mode, after modulated by the MPCGH, can be transformed into beams with an intensity-normalized differential signal, which can reflect the change trend of the aberration coefficients in the test wavefront. The experimental demonstration with designed MPCGHs in conjunction with two phase-only spatial light modulators was carried out to test the performance of the MWS.     

脉冲压缩光栅光路调节新方法研究

介绍了一种简单而实用的大口径脉冲压缩光栅光路调节方法,有效解决了普通光路调节方法中轴向调节精度不高的问题。首先由全息透镜(光栅)成像公式出发,推导出了该光路调节的基本原理。并从光栅记录系统与光栅衍射波像差的关系,结合初级像差理论推导得出叠栅条纹像差为0.4786λ,大约是光栅衍射波像差(0.25λ)的两倍,利用此关系也可对光栅衍射波像差进行实时监测。从数值模拟结果可知,利用叠栅条纹法调节光路可将光栅波像差减至0.06λ,相应的轴向误差量为0.007 mm,可有效提高了轴向调节精度。     

Direct amplitude detection of Zernike modes by computer-generated holographic wavefront sensor: Modeling and simulation

A fast holographic wavefront sensor is proposed using a computer-generated hologram (CGH). This CGH is a multiplexed hologram of different Zernike mode–amplitude combinations, and is designed in such a manner as to get the corresponding spots on the detector according to the presence and strength of a particular aberration. Interference between the aberrated wavefront (with a single mode–amplitude combination) and the Fourier transform of an image with single bright pixel (defined as dot image) is numerically calculated for one hologram. Different mode–amplitude combination and corresponding different positions of bright pixels (dots) are taken to compute various holograms and then all the holograms are multiplexed to get the final hologram. When the aberrated wavefront with a particular mode–amplitude combination is incident onto the multiplexed hologram, the corresponding dot is generated in the Fourier plane. A lens performs the Fourier transform in optical domain and provides the instant detection of amplitude of the respective Zernike mode. The main advantage of the scheme is to avoid the need of any computations, which makes it really fast. The simulation results are presented with the cross-talk analysis for few Zernike terms.     

无透镜傅里叶变换数字全息术中非共面误差的自动补偿算法

无透镜傅里叶变换数字全息术对活细胞进行显微相衬成像测量时,由于细胞处于运动状态导致记录物体与参考点源难以共面,从而引入成像误差.本文提出了一种有效可靠的基于位相分布的自动对焦再现算法,只需通过一幅全息图,即可确定出记录距离的优化数值解,并结合一次位相线性拟合,成功补偿掉非共面误差.将这种算法应用到活体宫颈癌细胞的相衬成像实验中获得了较为理想的实验结果,表明了这种自动补偿非共面误差算法的可行性. 关键词： 数字全息 无透镜傅里叶变换全息 自动对焦 位相畸变校正     

振幅型复合计算全息模式波前传感理论及验证

全息模式波前传感器是利用复合全息元件对待测波前中像差模式进行直接测量的一种新型波前传感器,其中的复合全息元件可编码设计多阶Zernike像差模式。提出一种有效方案以编码设计振幅型复合计算全息元件,该方案通过将多个编码有某阶Zernike像差模式的透过率调制扭曲光栅叠加产生振幅型计算全息元件。在小像差近似条件下推导了传感器输出信号与Zernike像差模式系数的解析关系。编码设计了振幅型复合全息图,并研究了其对单阶和多阶Zernike像差模式的探测性能。利用液晶光阀加载复合全息图进行了验证实验。所得实验结果与数值模拟结果基本一致。     

Image magnified lensless holographic projection by convergent spherical beam illumination

In liquid crystal spatial light modulator(SLM)-based holographic projection, the image is usually displayed at a distant projection screen through free space diffraction from a computer-generated hologram(CGH). Therefore,it allows for removing of the projection lens for the sake of system simplification and being aberration free, known as the "lensless holographic projection". However, the maximum size of the optical projected image is limited by the diffraction angle of the SLM. In this Letter, we present a method for the implementation of image magnification in a lensless holographic projection system by using convergent spherical wave illumination to the SLM.The complete complex amplitude of the image wavefront is reconstructed in a lensless optical filtering system from a phase-only CGH that is encoded by the off-axis double-phase method. The dimensions of the magnified image can break the limitation by the maximum diffraction angle of the SLM at a given projection distance.Optical experiment results with successful image magnification in the lensless holographic projection system are presented.     

An approach for sensing marine plankton using digital holographic imaging

The goal of this paper is to use digital holographic imaging for sensing marine plankton in recording sampling volume. The process stage of this approach includes: wavefront recording using in-line holographic recording set up and numerical reconstruction using Fresnel approximation and convolution algorithm. So, by capturing hologram of marine plankton and reconstructing hologram, the recorded optical field of marine plankton is retrieved. Digital holographic imaging is an extremely powerful technique for the study of marine plankton fields as it allows instantaneous, noninvasive, high-resolution recording of substantial volumes. Finally, this paper presents that it is possible for digital holographic imaging system to sense marine plankton according to laboratory results.     

Digital holographic microscopy for automated 3D cell identification: an overview (Invited Paper)

Digital holographic （DH） microscopy is a promising technique for quantitative phase contrast imaging. It provides complex amplitude of the object wavefront, which in turn yields the thickness distribution of the object. An added advantage of the technique is its ability for numerical focusing, which provides the thickness distribution of the object at different axial planes. In this invited paper, we present an overview of our reported work on two beam DH microscopyto acquire different cell parameters for cell imaging and automated cell identification. Applications to automated cells and automated identification of malaria infected red monitoring of stem cells without destroying the blood ceils are discussed.     

Wave-front curvature compensation of polarization phase-shifting digital holography

An on-axis polarization phase-shifting digital holographic microscope, based on a normal upright optical microscope, is constructed to quantitatively measure both the amplitude and phase distributions of specimen. The condenser lens and microscope objective employed in the object beam path enhance the illumination and magnification of the image, however, they induce additional phase aberration of the object wave. The physical formation of the phase aberration is theoretically analyzed, and a formula for the object wave front involving the phase aberration in the CCD plane is derived. The phase aberration can be eliminated in the reconstruction procedure by measuring a specimen-free hologram and then fitting the aberration phase with a least square ellipsoidal model to determine the parameters of the system. This phase aberration compensation procedure also reduces some of noises in the reconstructed phase of the specimen. The practicability of this method is demonstrated by a test experiment on microlenses.     

全息曝光系统轴向调节误差对光栅衍射波像差的影响

光栅衍射波像差作为全息光栅重要的技术指标之一,直接影响光栅分辨率,其中曝光光学系统的调节误差是引起光栅衍射波像差的主要因素。采用q参数讨论了高斯光束在光栅曝光光学系统中的传播和变换,通过计算高斯光束经准直系统后的相位给出了叠栅条纹相位分布的解析表达式,由此系统分析了曝光系统调节误差与光栅衍射波像差的关系。理论分析结果表明:左右曝光光路准直系统的相对离焦对光栅衍射波像差的影响最为显著;相对离焦量相同时,光栅衍射波像差随曝光系统焦距的减小而逐渐增大;理论模拟的条纹分布与实验中获得的叠栅条纹能够很好吻合。     

数字全息干涉术用于微波等离子体推进器羽流场的研究

利用数字全息干涉术研究了微波等离子体推进器羽流场的分布特征,由所记录的羽流场的数字全息图,经数值再现,得到了羽流场的相位分布,进而计算出等离子体羽流场的电子数密度.通过相位倍增法增加了羽流场的干涉条纹密度,从而能够更直观地反映出羽流场的折射率分布.结果表明,数字全息干涉术是研究微波等离子体推进器羽流场的一种有效方法.     

泽尼克多项式校正全息阵列光镊像差的实验研究

像差会影响光镊对粒子的捕获效果. 全息阵列光镊中, 像差不仅来自光学元件, 由特定算法设计的光阱相位片也会在光路中引入像差. 本文通过液晶空间光调制器加载泽尼克多项式相位图, 对全息阵列光镊中由光栅透镜组型算法引起的像差进行校正. 结果显示: 利用三阶泽尼克多项式可有效消除光路中由光栅透镜组型算法引 起的慧差, 使得捕获2 μm聚苯乙烯小球的阵列光阱刚度提高了约40%; 对比不同项的像差校正结果发现, 全息阵列光镊中由算法引起的慧差 与光学元件引起的像差一样, 也会对阵列光阱的捕获效果产生较大影响; 同时根据一阶像差校正结果可得光栅透镜 组型算法对于一阶泽尼克像差具有鲁棒性. 实验结果表明, 对全息阵列光镊中由 算法引起的像差进行校正, 对于提高光阱的捕获效果和深化对算法特性的认识都具有重要意义.     

双波长全息术实验研究及在自适应光学中的应用

在双波长全息术中,全息图可用一个波长记录,用另一个波长重现。用光学寻址液晶空间光调制器作为记录介质,可方便实现动态双波长全息图的记录。经一个畸变波前记录动态全息图后,由具有相同畸变的另一个波前去重现该动态全息图,可以实现对光学波前畸变的等比例缩小。在用于自适应光学的负性反馈回路中时,可简化大波前畸变的测量过程,可在中红外光谱范围内记录并制作动态全息图畸变校正器(在此波段直接的全息记录是极难的),也能增大可校正的畸变范围。介绍了这种动态干涉仪的实验实现,及该动态全息记录技术应用于自适应光学的前景。