共查询到8条相似文献,搜索用时 0 毫秒
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This study elucidated the frequency characteristics of series photodetector frequency circuit system for detection of DNA probe ET996 marked with fluorescence dye Cy5. We developed 48 MHz series photodetector frequency circuit system with good sensitivity for fluorescence detection. In accordance with the theory of series photodetector frequency circuit system, the frequency sensitivity can be improved by adjusting circuit parameters such as A (tan θ), Cq, C0, and Cp. In this research of A adjustment, the capacitance parameter Cm of 48 MHz series photodetector frequency circuit system was adjusted to improve the frequency sensitivity for detection of fluorescence dye concentration; moreover, the bias of photodetector was also adjusted to improve the frequency sensitivity. In the optimal conditions of capacitance match and photodetector bias, the detection limit of ET996-Cy5 fluorescence dye concentration 2 pmol/L can be measured by 48 MHz sensor system. The results of fluorescence experiment also demonstrated that the frequency shift of 48 MHz sensor system was linearly related to the logarithm of fluorescence dye concentration from 200 nmol/L to 2 pmol/L. The frequency method can be applied simply and the detection limit of ET996-Cy5 fluorescence dye concentration was lower than the conventional fluorescence technique by 2 orders. 相似文献
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《Ultrasonics sonochemistry》2014,21(5):1900-1906
The efficiency of ultrasonic cleaning vessels cannot be measured directly in an easy way. In the presented work, a sensor is developed which quantitatively measures the ablation of a test layer. The sensor element is a quartz crystal which is coated with a sacrificial layer. Small changes in mass of this layer can be measured by a frequency shift of the crystal oscillation. For measurements, a 10 MHz AT-cut quartz crystal was used in a cleaning vessel working at 44.9 kHz. To determine the frequency shift by the ablation of the test layer, the quartz crystal was driven by a frequency generator sweeping the frequency in the range of the resonance frequency and a characteristic frequency was determined. The test layer which was applied to the quartz crystal consisted of silica microparticles suspended in varnish. In a preliminary experiment using a commercial cleaner it could be shown that significant changes in resonance frequency by cavitation effect could be detected. The initial frequency shift of the sacrificial layer is reproducible within 10%. The test layer can be adapted to the conditions of the cleaning vessel. By changing the electrical input power of the vessel, a threshold in the cavitation erosion was found. 相似文献
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基于KTP键合晶体采用Hansch-Couillaud频率锁定技术实现了双波长外腔同时共振,理论和实验上分别研究了基于键合KTP晶体的HC频率锁定方案. 研究表明,与采用单KTP晶体的结果相比,采用键合KTP晶体进行HC锁频时,能将激光频率分别锁定到e1光或e2光的共振峰值. 实验中将环形腔腔模频率锁定到938nm激光器的输出频率上,1583nm激光器的输出频率锁定到环形腔腔模频率上,从而实现了三者之间的相位关联锁定.
关键词:
键合KTP晶体
Hansch-Couillaud锁频
双波长外腔共振 相似文献
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Mohamed E. Mostafa 《Proceedings of the Combustion Institute》2019,37(3):3053-3060
Fluorescence interference in Raman spectrum is a big barrier for rapid and precise analysis of coal structures by Raman spectroscopy. Dealing with fluorescence interference suitably is one of the key tasks before efficient application of Raman spectroscopy in coal assessment. In this study, Raman spectra and coal combustion characteristics of 32 kinds of Chinese coals were respectively obtained in a micro-Raman spectrometer and Thermal Gravimetric Analyzer. The degree of fluorescence interference in Raman spectrum was firstly defined and quantified as the drift coefficient α using a simple method without curve-fitting the spectrum. The correlations between the degree of fluorescence interference and coal property, coal combustion characteristics were set up and multivariable analysis was done. The results indicate that the degree of fluorescence interference is well related to the coal structures, and it is synthetically determined by volatile, moisture and ash content in coal. With the increase of volatile, moisture content in coal, the fluorescence interference increases continuously, and it can be reduced but not eliminated by drying the moisture in coals. Significant mathematical relations between the drift coefficient α and volatile, moisture content, coal combustion characteristic temperatures have been found. Coal with more evident fluorescence interference in Raman spectrum usually has lower degree of coalification, more polar functional groups, and burns at a lower temperature. The drift coefficient α can act as an efficient probe for coal property and coal combustion characteristics. This study provided a new and simple approach for evaluating coal property and coal combustion characteristics by fluorescence interference in Raman spectrum. 相似文献
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