共查询到20条相似文献,搜索用时 140 毫秒
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用性能稳定的薄层色谱用β-环糊精(β-CD)、硅胶手性固定相拆分手性药物对映体.在适当比例的乙腈-1%乙酸三乙胺(TEAA)、己烷-异丙醇、乙腈-甲醇-乙酸-三乙胺、甲醇-1%TEAA及乙腈-1%TEAA-三乙胺溶剂系统中展开,8种临床常用的手性药物对映体得到有效分离,对映异构体之间的相对比移值α为1.53~4.89. 相似文献
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本文报道了一种小型甾体类天然产物~(13)C-NMR数据库及检索系统。系统具有名称、结构、化学位移和分子式等多种检索功能。能有效地辅助人们对甾体类天然产物~(13)C-NMR图谱的解析。系统采用BASIC语言编写,编译后运行。经在IBM PC/XT机上运行得到了比较满意的结果。 相似文献
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甾体化合物的分光光度法测定 总被引:17,自引:0,他引:17
1引言甾体化合物及其制剂是一类重要药物。已见报道的定量分析方法均存在局限性,或者需要提纯,或是要依赖分子中的特殊官能团,这使得分析结果误差较大,或对结构不太清楚的植物提取物难以测定。由于甾体化合物的共同结构环戊烷多氢菲可以发生Liebermann-Barcharad反应,产生颜色。我们依据这一原理,建立了一般的甾体化合物分光光度测定法。2实验部分2.1仪器与试剂721分光光度计(上海第三分析仪器厂)。甾体化合物,用黄姜(华中农业大学植物园提供)经酸水解,120号汽油提取,活性炭脱色,蒸发烘干后备用。其中含有甾体皂甙,延令草… 相似文献
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药物分析中薄层色谱的方法认证 总被引:2,自引:0,他引:2
在药物分析中,针对所要求的性能参数,对一个薄层色谱程序的各个环节必须进行的认证方法和认可标准进行了讨论。建议当提出结果报告时,应附上关于对方法的认证参数和认证方法的说明。 相似文献
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在高效硅胶薄层板上分离了牛蒡子中4种2,3-苄基丁内酯型木脂素──牛蒡甙元(I)、牛蒡素B(Ⅱ)、牛蒡酚A(Ⅲ)、牛蒡酚F(Ⅳ)。流动相为二氯甲烷-乙醚-甲醇-己烷(4:1:0.4:1),室温放置,自然显色。薄层扫描法定量,各木脂素的线性范围在0.451~4.608μg,回收率为99.90%~100.90%,并用此法测定了不同产地牛蒡子中木脂素的含量。方法简便、快速,样品用量少。 相似文献
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《Analytical letters》2012,45(3):189-193
Abstract The separation of the chloroplast pigments is achieved by two-way chromatography on cellulose sheets. The chromatographic system employed is superior to any other thin-layer or paper system yet found. 相似文献
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薄层扫描法测定冬眠过程中刺猬活性组织酶的周期变化冯金城(天津师范大学天津300074)孙金生(天津水产研究所天津300221)1前言为了跟踪观察刺猬在冬眠过程中体内乳酸脱氢酶的周期性变化,利用薄层扫描仪测定了其相关的组织同工酶的差异。实践证明,此法简... 相似文献
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Naohiro Oshima Maho Saito Mina Niino Yuki Hiraishi Kana Ueki Kazuki Okoshi Takashi Hakamatsuka Noriyasu Hada 《Molecules (Basel, Switzerland)》2022,27(3)
To elucidate the interactions between crude drugs in Kampo medicines (traditional Japanese medicines), it is important to determine the content of the constituents in a cost-effective and simple manner. In this study, we quantified the constituents in crude drug extracts using thin-layer chromatography (TLC), an inexpensive and simple analytical method, to elucidate the chemical interactions between crude drugs. We focused on five crude drugs, for which quantitative high-performance liquid chromatography (HPLC) methods are stipulated in the Japanese Pharmacopoeia XVIII (JP XVIII) and compared the analytical data of HPLC and TLC, confirming that the TLC results corresponded with the HPLC data and satisfied the criteria of JP XVIII. (Z)-ligustilide, a major constituent in Japanese Angelica Root, for which a method of quantification has not been stipulated in JP XVIII, was also quantitatively analyzed using HPLC and TLC. Furthermore, Japanese Angelica Root was combined with 26 crude drugs to observe the variation in the (Z)-ligustilide content from each combination by TLC. The results revealed that combinations with Phellodendron Bark, Citrus Unshiu Peel, Scutellaria Root, Coptis Rhizome, Gardenia Fruit, and Peony Root increased the (Z)-ligustilide content. Quantifying the constituents in crude drug extracts using the inexpensive and simple TLC method can contribute to elucidating interactions between crude drugs in Kampo medicines, as proposed by the herbal-pair theory. 相似文献
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《Analytical letters》2012,45(1):21-33
Abstract Sterically hindered 17α-hydroxyl groups in steroids are silylated by trimethylsilylimidazole (TSIM) at 100° C. Decreasing rates of reaction were found for the following structures: 17α, 20β, 21-triol > 17α, 20α-diol > 17α, 20α, 21-triol. When methoxime derivatives are formed as the first step, HCL present in the excess of reagent catalyses the silylation reaction of hydroxyl groups. A simple procedure is described whereby methoxime (MO) derivatives are formed in 15 minutes at 60° C (the 11-one group does not react under these conditions) and persilylated compounds are then prepared in 2 hours at 100° C by reaction with trimethylsilylimidazole. 相似文献