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1.
Microbial degradation experiments were performed with each standard arsenical [arsenobetaine, trimethylarsine oxide, dimethylarsinic acid, methanearsonic acid, inorganic arsenic(V) and inorganic arsenic(III)]. As typical origins for marine micro-organisms, sediments, macro-algae, mollusc intestine and suspended substances were used. The results were from these experiments led us to the following conclusions: (1) there is an arsenic cycle which begins with the methylation of inorganic arsenic on the route to arsenobetaine and terminates with the complete degradation of arsenobetaine to inorganic arsenic; (2) all the organoarsenic compounds which are derived from inorganic arsenic in seawater, through the food chains, have the fate that they, at least in part, finally return to the original inorganic arsenic. 相似文献
2.
Dr. Hiroaki Imoto Prof. Dr. Kensuke Naka 《Chemistry (Weinheim an der Bergstrasse, Germany)》2019,25(8):1883-1894
Organoarsenic chemistry was actively studied until the middle of 20th century. Although various properties of organoarsenic compounds have been computationally predicted, for example, frontier orbital levels, aromaticity, and inversion energies, serious concern to the danger of their synthetic processes has restricted experimental studies. Conventional synthetic routes require volatile and toxic arsenic precursors. Recently, nonvolatile intermediate transformation (NIT) methods have been developed to safely access functional organoarsenic compounds. Important intermediates in the NIT methods are cyclooligoarsines, which are prepared from nonvolatile inorganic precursors. In particular, the new approach has realized experimental studies on conjugated arsenic compounds: arsole derivatives. The elucidation of their intrinsic properties has triggered studies on functional organoarsenic chemistry. As a result, various kinds of arsenic-containing π-conjugated molecules and polymers have been reported for the last few years. In this minireview, progress of this recently invigorated field is overviewed. 相似文献
3.
An analytical method based on microwave decomposition and flow injection analysis (FIA) coupled to hydride generation atomic absorption spectrometry (HGAAS) is described. This is used to differentiate arsenite [As(III)], arsenate [As(V)], monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA) from organoarsenic compounds usually present in seafood. Without microwave digestion, direct analysis of urine by HGAAS gives the total concentration of As(III), As(V), MMA and DMA because organoarsenic compounds such as arsenobetaine, usually found in most seafood, are not reducible upon treatment with borohydride and therefore cannot be determined by using the hydride generation technique. The microwave oven digestion procedure with potassium persulfate and sodium hydroxide as decomposition reagents completely decomposes all arsenicals to arsenate and this can be measured by HGASS. Microwave decomposition parameters were studied to achieve efficient decomposition and quantitative recovery of arsenobetaine spiked into urine samples. The method is applied to the determination of urinary arsenic and is useful for the assessment of occupational exposure to arsenic without intereference from excess organoarsenicals due to the consumption of seafood. Analysis of urine samples collected from an individual who ingested some seafood revealed that organoarsenicals were rapidly excreted in urine. After the ingestion of a 500-g crab, a 10-fold increase of total urinary arsenic was observed, due to the excretion of organoarsenicals. The maximum arsenic concentration was found in the urine samples collected approximately between 4 to 17 hr after eating seafood. However, the ingestion of organoarsenic-containing seafoods such as crab, shrimp and salmon showed no effect on the urinary excretion of inorganic arsenic, MMA and DMA. 相似文献
4.
Maria Jose Ruiz-Chancho Jose Fermín López-Sánchez 《International journal of environmental analytical chemistry》2013,93(9):844-855
Arsenic compounds were determined in extracts of branches, leaves and roots from plants growing in a mining contaminated area. The selected species were Dryopteris filix-max, Quercus pubescens, Dipsacus fullonum, Alnus glutinosa, Buxus sempervirens and Brachythecium cf. reflexum. Total arsenic content in the subsamples was analysed by ICPMS after acidic digestion. In general, concentrations in the plant parts followed the gradient roots?>?branches?>?leaves indicating that they are arsenic-resistant plants. Arsenic species were determined in water/methanol (9?+?1, v/v) extracts by HPLC-ICPMS. Different levels of organoarsenicals were found depending on plant part and plant species. Higher percentages of organoarsenic compounds were recorded in branches and leaves (up to 35% in the boxtree sample), than in roots (0.7–5.2% in the same plant species). The absence of organic arsenic species in the soil where the plants were collected and the low levels of organoarsenicals found in the roots, indicate that the studied plants have the ability to accumulate or synthesise organoarsenic compounds in relatively high percentages, and this information contributes to enlarge the knowledge of arsenic uptake and speciation in plants. 相似文献
5.
A single quadrupole high performance liquid chromatography electrospray mass spectrometry system with a variable fragmentor voltage facility was used in the positive ion mode for simultaneous recording of elemental and molecular mass spectral data for arsenic compounds. The method was applicable to the seven organoarsenic compounds tested: four arsenic-containing carbohydrates (arsenosugars), a quaternary arsonium compound (arsenobetaine), dimethylarsinic acid, and dimethylarsinoylacetic acid. It was not suitable for the two inorganic arsenic species arsenite and arsenate. In the case of arsenosugars, qualifying ion data for a characteristic common fragment (m/z 237) was also simultaneously obtained. The method was used to identify and quantify the major arsenosugars in crude extracts of two brown algae. 相似文献
6.
Jaafar J Irwan Z Ahamad R Terabe S Ikegami T Tanaka N 《Journal of separation science》2007,30(3):391-398
An online preconcentration technique by dynamic pH junction was studied to improve the detection limit for anionic arsenic compounds by CE. The main target compound is roxarsone, or 3-nitro-4-hydroxyphenylarsonic acid, which is being used as an animal feed additive. The other inorganic and organoarsenic compounds studied are the possible biotransformation products of roxarsone. The arsenic species were separated by a dynamic pH junction in a fused-silica capillary using 15 mM phosphate buffer (pH 10.6) as the BGE and 15 mM acetic acid as the sample matrix. CE with UV detection was monitored at a wavelength of 192 nm. The influence of buffer pH and concentration on dynamic pH junction were investigated. The arsenic species focusing resulted in LOD improvement by a factor of 100-800. The combined use of C18 and anion exchange SPE and dynamic pH junction to CE analysis of chicken litter and soils helps to increase the detection sensitivity. Recoveries of spiked samples ranged between 70 and 72%. 相似文献
7.
Cation exchange and anion exchange liquid chromatography were coupled to an ICP-MS and optimised for the separation of 13 different arsenic species in body fluids (arsenite, arsenate, dimethylarsinic acid (DMAA), monomethylarsonic acid (MMAA), trimethylarsine oxide (TMAO), tetramethylarsonium ion (TMA), arsenobetaine (AsB), arsenocholine (AsC), dimethylarsinoyl ethanol (DMAE) and four common dimethylarsinoylribosides (arsenosugars). The arsenic species were determined in seaweed extracts and in the urine and blood serum of seaweed-eating sheep from Northern Scotland. The sheep eat 2-4 kg of seaweed daily which is washed ashore on the most northern Island of Orkney. The urine, blood and wool of 20 North Ronaldsay sheep and kidney, liver and muscle from 11 sheep were sampled and analysed for their arsenic species. In addition five Dorset Finn sheep, which lived entirely on grass, were used as a control group. The sheep have a body burden of approximately 45-90 mg arsenic daily. Since the metabolism of arsenic species varies with the arsenite and arsenate being the most toxic, and organoarsenic compounds such as arsenobetaine the least toxic compounds, the determination of the arsenic species in the diet and their body fluids are important. The major arsenic species in their diet are arsenoribosides. The major metabolite excreted into urine and blood is DMAA (95 +/- 4.1%) with minor amounts of MMAA, riboside X, TMA and an unidentified species. The occurrence of MMAA is assumed to be a precursor of the exposure to inorganic arsenic, since demethylation of dimethylated or trimethylated organoarsenic compounds is not known (max. MMAA concentration 259 microg/L). The concentrations in the urine (3179 +/- 2667 microg/L) and blood (44 +/- 19 microg/kg) are at least two orders of magnitude higher than the level of arsenic in the urine of the control sheep or literature levels of blood for the unexposed sheep. The tissue samples (liver: 292 +/- 99 microg/kg, kidney: 565 +/- 193 microg/kg, muscle: 680 +/- 224 microg/kg) and wool samples (10470 +/- 5690 microg/kg) show elevated levels which are also 100 times higher than the levels for the unexposed sheep. 相似文献
8.
J. Feldmann Katie John Paramee Pengprecha 《Analytical and bioanalytical chemistry》2000,368(1):116-121
Cation exchange and anion exchange liquid chromatography were coupled to an ICP-MS and optimised for the separation of 13
different arsenic species in body fluids (arsenite, arsenate, dimethylarsinic acid (DMAA), monomethylarsonic acid (MMAA),
trimethylarsine oxide (TMAO), tetramethylarsonium ion (TMA), arsenobetaine (AsB), arsenocholine (AsC), dimethylarsinoyl ethanol
(DMAE) and four common dimethylarsinoylribosides (arsenosugars). The arsenic species were determined in seaweed extracts and
in the urine and blood serum of seaweed-eating sheep from Northern Scotland. The sheep eat 2–4 kg of seaweed daily which is
washed ashore on the most northern Island of Orkney. The urine, blood and wool of 20 North Ronaldsay sheep and kidney, liver
and muscle from 11 sheep were sampled and analysed for their arsenic species. In addition five Dorset Finn sheep, which lived
entirely on grass, were used as a control group. The sheep have a body burden of approximately 45–90 mg arsenic daily. Since
the metabolism of arsenic species varies with the arsenite and arsenate being the most toxic, and organoarsenic compounds
such as arsenobetaine the least toxic compounds, the determination of the arsenic species in the diet and their body fluids
are important. The major arsenic species in their diet are arsenoribosides. The major metabolite excreted into urine and blood
is DMAA (95 ± 4.1%) with minor amounts of MMAA, riboside X, TMA and an unidentified species. The occurrence of MMAA is assumed
to be a precursor of the exposure to inorganic arsenic, since demethylation of dimethylated or trimethylated organoarsenic
compounds is not known (max. MMAA concentration 259 μg/L). The concentrations in the urine (3179 ± 2667 μg/L) and blood (44
± 19 μg/kg) are at least two orders of magnitude higher than the level of arsenic in the urine of the control sheep or literature
levels of blood for the unexposed sheep. The tissue samples (liver: 292 ± 99 μg/kg, kidney: 565 ± 193 μg/kg, muscle: 680 ±
224 μg/kg) and wool samples (10 470 ± 5690 μg/kg) show elevated levels which are also 100 times higher than the levels for
the unexposed sheep.
Received: 29 February 2000 / Revised: 26 April 2000 / Accepted: 1 May 2000 相似文献
9.
Decomposition processes of organoarsenic compounds significantly influence arsenic cycles in aquatic environments, and such processes depend on bacterial activity. However, the bacterial characteristics in these environments are obscure. Accordingly, we observed seasonal variations of arsenic species and the bacterial population decomposing dimethylarsinic acid (DMAA) in Lake Kahokugata from April 2002 to January 2003. Monitoring of bacterial biomass involving DMAA decomposition using the most probable number procedure showed that the bacterial cell densities ranged from 36 to 3600 ml?1. On the other hand, methylated arsenic was not detected during the experimental period, although the inorganic arsenic concentration was over 4 nM . This suggests that bacteria remineralized methylated arsenic species to inorganic arsenic. Furthermore, the composition of bacterial communities involving DMAA decomposition was examined by restriction‐fragment‐length polymorphism analysis of the 16S rDNA nucleotide. As a result, a total of 49 isolates were classified into 10 type groups, and 32 of these isolates belonged to three dominant type groups. Phylogenetic analysis using 16S rDNA partial sequences (ca 320 bp) suggests that the representative isolates of the dominant type groups are specific to the summer or winter season. Moreover, as a result of the culture experiments to examine DMAA decomposition activity, the representative isolates decomposed 1 µM DMAA at a decomposition percentage of below 80%. In conclusion, some bacterial communities in a specific season can decompose DMAA to varying degrees, contributing to the annual cycle of arsenic species. Copyright © 2005 John Wiley & Sons, Ltd. 相似文献
10.
Kidneys of clams of the genus Tridacna accumulate metabolic products from symbiotic unicellular algae that grow in the mantles of the clams. These metabolites include organoarsenic compounds that are biosynthesised by algae from arsenate in seawater. The arsenic compounds in aqueous extracts of the kidney of the giant clam T. derasa were investigated by liquid chromatography/electrospray ionisation mass spectrometry. About 50% of the water-soluble arsenic was present as dimethylarsinoylribosides and dimethylarsinate which are common algal metabolites. The major compound in the kidney (50% of water-soluble arsenic) was identified as a 5-dimethylarsinoyl-2,3,4-trihydroxycarboxylic acid, a new natural product. 相似文献
11.
Potential widespread applications of organoarsenic chemistry have been limited by the inherent lack of safe and effective As?C bond formation reactions. Several alternative reagents and methods have been developed in the last few decades to address the hazards and drawbacks associated with traditional arsenic synthetic strategies. Herein, this minireview summarizes the advances made in nucleophilic, electrophilic, radical and metal‐mediated As(III)?C bond formations while specifically highlighting the behavior of arsenic synthons with various well‐established reagents (eg. Grignard reagents, organolithium compounds, organometallic reagents, radical initiators and Lewis/Brønsted bases). Avenues for asymmetric synthesis are also discussed, as are recent advances in organoarsenic chemistry suggesting that arsines exhibit novel reactivities independent from that of other relatively more well explored Group V cogeners. 相似文献
12.
Yue Yang Zihui Liu Hao Chen 《International journal of environmental analytical chemistry》2019,99(1):87-100
A simple and fast method of low-density solvent based dispersive liquid-liquid microextraction (LDS-DLLME) followed by high-performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS) has been developed for the speciation analysis of organoarsenic and inorganic arsenic in water samples. The low-density solvent (octanol) was given as the organic phase and injected into the aqueous sample (donor phase) with methanol as the disperser. With As (V), As (III), p-APAA, 4-HPAA, ROX and PAA as target species, factors of LDS-DLLME including pH value, anionic carriers, elution conditions and extractant, were studied in detail. Besides, volumes of solvents were further optimised by response surface methodology. Under the optimal conditions, the limits of detection for four phenylarsenics and arsenate were in the range of 0.001–0.039 μg L?1. The relative standard deviations (RSDs) were 3.6–9.4% and the enrichment factors varied from 6.2 to 70.8. The proposed method of LDS-DLLME-HPLC-ICP-MS was satisfactorily applied to the determination of six arsenic compounds in water samples with recoveries of 81.8–111.7% for the spiked lake water samples. 相似文献
13.
Direct measurement of lipid-soluble arsenic species in biological samples with HPLC-ICPMS 总被引:2,自引:0,他引:2
Lipid-soluble arsenicals (arsenolipids) occur in a wide range of biological samples where they may play a key role in the biosynthesis of organoarsenic compounds from inorganic arsenic. The study of these compounds has been hindered, however, by the lack of a suitable analytical technique able to separate and measure the various lipid species. As a source of arsenolipids, we used 10 crude fish oils from various regions of the world. Total arsenic analyses on the fish oils, performed with ICPMS following acid digestion with microwave-assisted heating, gave concentrations from 4.3 to 10.5 mg As kg(-1). All of the arsenic was soluble in non-polar solvents such as hexane. Analysis of the fish oils for arsenolipids was performed by normal phase HPLC-ICPMS with various mixtures of organic solvents as mobile phases. Inherent problems of instability associated with the introduction of organic solvents to the plasma were overcome by the use of reduced column flow, a chilled spray chamber, and the addition of oxygen directly to the plasma. All ten fish oils appeared to contain the same 4-6 major arsenolipids, but in varying amounts depending on the origin of the fish. Further chromatography with both normal phase and reversed-phase conditions on some of the oils indicated the presence of many more minor arsenolipids. Quantification was achieved by external calibration against triphenylarsine oxide or triphenylarsine sulfide, and the sum of species following HPLC of the oils matched well the total arsenic results (92-107%). The method was applied to samples of food supplements (fish oil capsules) and a packaged food product (cod liver) whereby arsenolipids were measured and found to be significant arsenic constituents. This study represents the first attempt to directly measure intact arsenolipids and, with appropriate sample preparation, may be suitable for quantitative measurement of these arsenicals in a range of biological samples, including foodstuffs. 相似文献
14.
15.
The determination of arsenic by atomic-absorption spectrometry with use of a graphite-cloth ribbon placed inside various types of graphite tubes was investigated. It was found that the graphite-cloth ribbon greatly enhances the sensitivity for arsenic and reduces the interferences from various heavy metals, especially when it is placed inside a pyrolytic graphite tube and a nitric acid matrix is used. This is attributed to condensation of arsenic on the ribbon, owing to a temperature lag during the drying and ashing cycles, and to the formation of interlamellar compounds of arsenic with graphite. 相似文献
16.
It is shown that four different mercury species are formed by the abiotic reaction of inorganic mercury with different, ecologically relevant, organolead and organoarsenic compounds. Therefore solutions of tetraethyl-lead, trimethyl-lead chloride and dimethylarsonic acid were prepared and mixed with stock solutions of inorganic mercury at different concentrations. The final solutions were analyzed for their content of newly synthesized mercury species. The analysis was carried out by using a system of solid-phase micro-extraction (SPME): capillary gas chromatography (GC), microwave-induced plasma (MIP) and a plasma-emission detector (PED). We found that transfer of one or two alkyl groups to the inorganic mercury is possible under the conditions mentioned below. The transalkylation rate depends on the kind of organometallic compound and on the pH. The results were confirmed by the reaction with inorganic mercury and analysis of a soil sample, containing tetraethyl-lead and trimethyl-lead, in which not only the monoalkyl compound, but also the dialkylated compound of the relevant inorganic metal, were found. © 1997 John Wiley & Sons, Ltd. 相似文献
17.
Lívia Botelho de Abreu Rodinei Augusti Lucas Schmidt Valderi Luiz Dressler Erico Marlon de Moraes Flores Clésia Cristina Nascentes 《Analytical and bioanalytical chemistry》2013,405(24):7643-7651
The different chemical forms of arsenic compounds, including inorganic and organic species, present distinct environmental impacts and toxicities. Desorption electrospray ionization mass spectrometry (DESI-MS) is an excellent technique for in situ analysis, as it operates under atmospheric pressure and room temperature and is conducted with no/minimal sample pretreatment. Aimed at expanding its scope, DESI-MS is applied herein for the quick and reliable detection of inorganic (arsenate—As(V): AsO4 3? and arsenite—As(III): AsO2 ?) and organic (dimethylarsinic acid—DMA: (CH3)2AsO(OH) and disodium methyl arsonate hexahydrate: CH3AsO3·2Na·6H2O) arsenic compounds in fern leaves. Operational conditions of DESI-MS were optimized with DMA standard deposited on paper surfaces to improve ionization efficiency and detection limits. Mass spectra data for all arsenic species were acquired in both the positive and negative ion modes. The positive ion mode was shown to be useful in detecting both the organic and inorganic arsenic compounds. The negative ion mode was shown only to be useful in detecting As(V) species. Moreover, MS/MS spectra were recorded to confirm the identity of each arsenic compound by the characteristic fragmentation profiles. Optimized conditions of DESI-MS were applied to the analysis of fern leaves. LC-ICP-MS was employed to confirm the results obtained by DESI-MS and to quantify the arsenic species in fern leaves. The results confirmed the applicability of DESI-MS in detecting arsenic compounds in complex matrices. 相似文献
18.
Optimization of microwave‐assisted extraction for six inorganic and organic arsenic species in chicken tissues using response surface methodology
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Response surface methodology was applied to optimize the parameters for microwave‐assisted extraction of six major inorganic and organic arsenic species (As(III), As(V), dimethyl arsenic acid, monomethyl arsenic acid, p‐arsanilic acid, and roxarsone) from chicken tissues, followed by detection using a high‐performance liquid chromatography with inductively coupled mass spectrometry detection method, which allows the simultaneous analysis of both inorganic and organic arsenic species in the extract in a single run. Effects of extraction medium, solution pH, liquid‐to‐solid ratio, and the temperature and time of microwave‐assisted extraction on the extraction of the targeted arsenic species were studied. The optimum microwave‐assisted extraction conditions were: 100 mg of chicken tissue, extracted by 5 mL of 22% v/v methanol, 90 mmol/L (NH4)2HPO4, and 0.07% v/v trifluoroacetic acid (with pH adjusted to 10.0 by ammonium hydroxide solution), ramping for 10 min to 71°C, and holding for 11 min. The method has good extraction performance for total arsenic in the spiked and nonspiked chicken tissues (104.0 ± 13.8% and 91.6 ± 7.8%, respectively), except for the ones with arsenic contents close to the quantitation limits. Limits of quantitation (S/N = 10) for As(III), As(V), dimethyl arsenic acid, monomethyl arsenic acid, p‐arsanilic acid, and roxarsone in chicken tissues using this method were 0.012, 0.058, 0.039, 0.061, 0.102, and 0.240 mg/kg (dry weight), respectively. 相似文献
19.
A novel approach, ultrasound-assisted dispersive liquid–liquid microextraction combined with liquid chromatography–mass spectrometry (UA-DLLME with LC–MS) is demonstrated to be quite useful for the determination of trace amounts of organoarsenic compounds in edible oil. The organoarsenic compounds studied include dimethylarsinic acid (DMA), monomethylarsonic acid (MMA) and 3-nitro-4-hydroxyphenyl arsenic acid (Roxarsone). Orthogonal array experimental design (OAD) was utilized to investigate the parameter space of conditions for UA-DLLME. The optimum conditions were found to be 4 min of ultrasonic extraction using 1.25 mL of mixed solvent with 50 μL of buffer solution. Under these optimal conditions, the linear range was from 10 ng g−1 to 500 ng g−1 for DMA and Roxarsone, from 25 ng g−1 to 500 ng g−1 for MMA. Limits of detection of DMA, MMA and Roxarsone were 1.0 ng g−1, 3.0 ng g−1 and 5.8 ng g−1, respectively. The precisions and recoveries also were investigated by spiking 3-level concentrations in edible oil. The recoveries obtained were over 89.9% with relative standard deviation (RSD) of 9.6%. The new approach was utilized to successfully detect trace amounts of organoarsenic compounds in various edible oil samples. 相似文献
20.
Uriel Arroyo-Abad Jürgen Mattusch Sibylle Mothes Rainer Wennrich Frank-Michael Matysik 《Talanta》2010,82(1):38-162
Arsenic is a metalloid well known to be potentially toxic depending of its species. Lipid-soluble arsenicals (arsenolipids) are present in a wide range of biological samples in which they could play a role in the biosynthesis of organoarsenic compounds from inorganic arsenic compounds. Arsenolipids have recently attracted considerable interest. In order to gain deeper insights into the impact of arsenolipids new analytical approaches for reliable determination of this class of arsenic-containing hydrocarbons in various matrices are needed.High concentrations of arsenolipids were found in seafood which served as sample material in this study. We report the investigation of three arsenolipids found in canned cod liver from which they were extracted and purified by solid phase extraction (SPE) using a silica gel column and ethyl acetate/methanol as eluent. Analytical studies were conducted by means of gas chromatography coupled with ICP-MS, MIP-AES and EI-qMS and by TOF-MS. The results obtained by GC-ICP-MS and GC-MIP-AES showed the existence of numerous arsenic compounds in the SPE fractions collected. Three major peaks were found within a retention time window between 10 and 25 min. The presence of arsenic compounds in the fish tissue could be confirmed using GC-EI-qMS analysis. Corresponding information of the molecular weights of the major arsenic species were provided by TOF-MS which allows highly accurate mass determinations. The results showed the presence of the arsenic-containing hydrocarbons with the following molecular formulas: C17H37AsO (calculated for [M+H]+ 333.2133; found 333.2136; Δm = 0.90 ppm); C19H41AsO (calculated for [M+H]+ 361.2446; found 361.2446; Δm = 0.00 ppm); C23H37AsO (calculated for [M+H]+ 405.2133; found 405.2145; Δm = 2.96 ppm). Suggestions for the corresponding structures are discussed. 相似文献