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作为一种新型荧光纳米材料,量子点具有十分优异的光学特性,是分析化学、生物科学、医学等领域研究的热点标记材料。分子印迹聚合物是能够进行特异性识别和选择性吸附的“仿生”材料,它易于制备且具有较好的重现性和稳定性,因而分子印迹技术已成为具有广阔应用前景的识别技术。量子点基分子印迹荧光传感器结合了量子点和分子印迹技术的优势,由于其高选择性和高灵敏度,在环境监测、食品检测、生物分析等领域得到快速发展。但该传感器在应用中也还存在亲水性不足、识别单一、便携性不足等问题。该文引用了近5年来发表在American Chemical Society、Elsevier等数据库约20篇相关文献,对量子点基分子印迹荧光传感器的构建及该传感器在快速检测分析痕量物质中的应用进展进行了综述。首先根据荧光光谱图中发射峰个数的不同分别介绍了3种量子点基分子印迹荧光传感器的类型及相关识别机理,其次根据待测物的不同归纳介绍了近五年来该传感器在离子、有机小分子、生物大分子等检测分析中的最新研究进展,最后对当前该传感器在制备及应用中仍存在的问题进行了总结并对其发展趋势进行了展望。 相似文献
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由于光学性质独特,量子点成为近年来发展起来的极具应用前景的半导体纳米材料,作为荧光标记物在生物和化学领域备受关注。随着生物技术的发展,适配体以其高特异性、强亲和力等特点被用作生物探针或生物传感元件,在分析检测中得到广泛应用。将量子点与适配体结合构建的纳米生物识别体系,互补结合适配体和量子点的特殊性质,可实现对靶标物质的超灵敏、高选择性及快速检测。本文概述了量子点的合成、修饰及其与适配体的偶联,重点介绍了近几年基于量子点标记的适配体技术在生物分子、病原微生物、细胞、真菌毒素等方面的应用,并展望了该技术在分析检测领域的发展趋势。 相似文献
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量子点在生物化学分析中的应用 总被引:1,自引:0,他引:1
量子点(quantumdots,QDs)由于其优异的光学和电学特性,作为新型的荧光试剂探针对生物大分子进行标记,成为近年来迅速发展的纳米材料在生化分析领域的重要应用之一。文章简述了量子点的基本特性,对制备和修饰量子点的各种方法进行比较总结,重点阐述量子点在生物化学分析中的新进展,尤其是对生物大分子的识别和标记作了详细的总结,并提出研究中存在的一些待解决的问题以及今后量子点的研究方向。 相似文献
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The application of micro total analysis system (μTAS) has grown exponentially in the past decade. DNA analysis is one of the primary applications of μTAS technology. This review mainly focuses on the recent development of the polymeric microfluidic devices for DNA analysis. After a brief introduction of material characteristics of polymers, the various microfabrication methods are presented. The most recent developments and trends in the area of DNA analysis are then explored. We focus on the rapidly developing fields of cell sorting, cell lysis, DNA extraction and purification, polymerase chain reaction (PCR), DNA separation and detection. Lastly, commercially available polymer-based microdevices are included. 相似文献
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Solid‐phase extraction with the metal–organic framework MIL‐101(Cr) combined with direct analysis in real time mass spectrometry for the fast analysis of triazine herbicides
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Xianjiang Li Jiawei Xing Cuilan Chang Xin Wang Yu Bai Xiuping Yan Huwei Liu 《Journal of separation science》2014,37(12):1489-1495
MIL‐101(Cr) is an excellent metal–organic framework with high surface area and nanoscale cavities, making it promising in solid‐phase extraction. Herein, we used MIL‐101(Cr) as a solid‐phase extraction packing material combined with fast detection of direct analysis in real time mass spectrometry (DART‐MS) for the analysis of triazine herbicides. After systematic optimization of the operation parameters, including the gas temperature of DART, the moving speed of the 1D platform, solvent for desorption, amount of MIL‐101(Cr) extraction time, eluent volume and salt concentration, this method can realize the simultaneous detection of five kinds of triazine herbicides. The limits of detection were 0.1~0.2 ng/mL and the linear ranges covered more than two orders of magnitude with the quantitation limits of 0.5~1 ng/mL. Moreover, the developed method has been applied for the analysis of lake water samples and the recoveries for spiked analytes were in the range of 85~110%. These results showed that solid‐phase extraction with metal–organic frameworks is an efficient sample preparation approach for DART‐MS analysis and could find more applications in environmental analysis. 相似文献
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Guoqing Wang Chaesung Lim Lingxin Chen Hyangah Chon Jaebum Choo Jongin Hong Andrew J. deMello 《Analytical and bioanalytical chemistry》2009,394(7):1827-1832
We report a new method for the trace analysis of mercury (II) ions in water. The approach involves the use of droplet-based
microfluidics combined with surface-enhanced Raman scattering (SERS) detection. This novel combination provides both fast
and sensitive detection of mercury (II) ions in water. Specifically, mercury (II) ion detection is performed by using the
strong affinity between gold nanoparticles and mercury (II) ions. This interaction causes a change in the SERS signal of the
reporter molecule rhodamine B that is a function of mercury (II) ion concentration. To allow both reproducible and quantitative
analysis, aqueous samples are encapsulated within nanoliter-sized droplets. Manipulation of such droplets through winding
microchannels affords rapid and efficient mixing of the contents. Additionally, memory effects, caused by the precipitation of nanoparticle aggregates on channel walls, are removed since the aqueous droplets are completely
isolated by a continuous oil phase. Quantitative analysis of mercury (II) ions was performed by calculating spectral peak
area of rhodamine B at 1,647 cm−1. Using this approach, the calculated concentration limit of detection was estimated to be between 100 and 500 ppt. Compared
with fluorescence-based methods for the trace analysis of mercury (II) ions, the detection sensitivities were enhanced by
approximately one order of magnitude. The proposed analytical method offers a rapid and reproducible trace detection capability
for mercury (II) ions in water. 相似文献
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建立奥曲肽的高效液相色谱定量分析方法。色谱柱为Eclipse plus C18柱(4.6 mm×250 mm,5 μm),流动相为乙腈-0.25%高氯酸水溶液(体积比为30∶70),流量为1.0 mL/min,检测波长为210 nm,柱温为25℃。奥曲肽的质量浓度在4.38~219 μg/mL范围内与色谱峰面积成良好的线性关系,相关系数为0.9999,检出限为1.1 ng,定量限为2.19 ng。测定结果的相对标准偏差为0.26%~0.46% (n=5),加标回收率为97.41%~100.26%。该方法简便、快速、准确,适用于奥曲肽原料药与制剂的定量分析。 相似文献
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Laser-induced breakdown spectroscopy for detection of explosives residues: a review of recent advances, challenges, and future prospects 总被引:2,自引:0,他引:2
Jennifer L. Gottfried Frank C. De Lucia Jr Chase A. Munson Andrzej W. Miziolek 《Analytical and bioanalytical chemistry》2009,395(2):283-300
In this review we discuss the application of laser-induced breakdown spectroscopy (LIBS) to the problem of detection of residues
of explosives. Research in this area presented in open literature is reviewed. Both laboratory and field-tested standoff LIBS
instruments have been used to detect explosive materials. Recent advances in instrumentation and data analysis techniques
are discussed, including the use of double-pulse LIBS to reduce air entrainment in the analytical plasma and the application
of advanced chemometric techniques such as partial least-squares discriminant analysis to discriminate between residues of
explosives and non-explosives on various surfaces. A number of challenges associated with detection of explosives residues
using LIBS have been identified, along with their possible solutions. Several groups have investigated methods for improving
the sensitivity and selectivity of LIBS for detection of explosives, including the use of femtosecond-pulse lasers, supplemental
enhancement of the laser-induced plasma emission, and complementary orthogonal techniques. Despite the associated challenges,
researchers have demonstrated the tremendous potential of LIBS for real-time detection of explosives residues at standoff
distances.
Figure This review discusses the application of laser-induced breakdown spectroscopy (LIBS) to the problem of explosive residue detection.
LIBS offers the capability for real-time, standoff detection of trace amounts of residue explosives on various surfaces 相似文献
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Annija Lace Aideen Byrne Simon Bluett Laurent Malaquin Vincent Raimbault Remi Courson Zain Hayat Breda Moore Eoin Murray 《Journal of separation science》2022,45(5):1042-1050
An ion chromatography system employing a low-cost three-dimensional printed absorbance detector for indirect ultraviolet detection towards portable phosphate analysis of environmental and industrial waters has been developed. The optical detection cell was fabricated using stereolithography three-dimensional printing of nanocomposite material. Chromatographic analysis and detection of phosphate were carried out using a CS5A 4 × 250 mm analytical column with indirect ultraviolet detection using a 255 nm light-emitting diode. Isocratic elution using a 0.6 mM potassium phthalate eluent combined with 1.44 mM sodium bicarbonate was employed at a flow rate of 0.75 mL/min. A linear calibration range of 0.5 to 30 mg/L PO43− applicable to environmental and wastewater analysis was achieved. For retention time and peak area repeatability, relative standard deviation values were 0.68 and 4.09%, respectively. Environmental and wastewater samples were analyzed with the optimized ion chromatography platform and the results were compared to values obtained by an accredited ion chromatograph. For the analysis of environmental samples, relative errors of <14 % were achieved. Recovery analysis was also carried out on both freshwater and wastewater samples and recovery results were within the acceptable range for water analysis using standard ion chromatography methods. 相似文献
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《Electroanalysis》2005,17(13):1215-1221
The coupling of a capillary electrophoresis (CE) microchip with electrochemical detection with a chemometric approach was used for the classification of wines. Cabernet Sauvignon wine samples from 3 different geographical regions were used for verifying the discriminating power of the microchip electrophoretic technique using high separation voltage (3000 V) with analysis time of only 60 seconds. Electrophoretic data were transformed into initial 14 variables, concerning peak's area and peak's height. Principal component analysis was applied to optimize the information given by the electrophoretic data sets. Results of classification using LDA and CART were promising and the preliminary model was able to classify samples on the basis of their geographical origin. While the coupling of microchips with chemometrics approaches is illustrated for a former tentative of the classification of wines it could be readily extended to a wide range of other important applications. 相似文献
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Mohamed Hemida Alireza Ghiasvand Mirek Macka Vipul Gupta Paul R. Haddad Brett Paull 《Journal of separation science》2023,46(15):2300283
Liquid chromatography is a prominent analytical technique in separation science and chemical analysis, applied across numerous fields of research and within industrial applications. Over the past few decades, there has been a growing interest in the miniaturization of this technique, which has been particularly enabled through new miniature and portable detection technologies for in-field, at-site, and point-of-need (collectively ‘out-of-lab’) analyses. Accordingly, significant advances have been made in recent years in the development of miniaturized liquid chromatography with photometric, electrochemical, and mass spectrometric detection, enabling the development of field-deployable and portable instruments for various applications. Herein, recent developments in the miniaturization of detection systems for inclusion within, and/or coupling with, portable liquid chromatographic systems, are reviewed in detail together with critical comments and expected future trends in this area. 相似文献