共查询到20条相似文献,搜索用时 15 毫秒
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ZHANG Ying JIN Wei YANG Meng-chao ZHANG Ti-qiang ZHOU Chao XIE Fei SONG Qi REN Hao JIN Qin-han MU Ying 《高等学校化学研究》2012,28(5):792-796
A method of using Au colloid to capture the decomposed product of penicillin, penicillamine, on a surface plasmon resonance(SPR) biosensor for the quantitative determination of penicillin was developed. Based on the decomposition of penicillin to generate penicillamine and penilloaldehyde, a high sensitive biosensor for detecting penicillin was also developed. In our experiment, it was penicillamine rather than penicillin that has been measured. This is because penicillamine contains a functional group that makes it self-assembling on Au colloid to increase the molecular weight so as to improve the surface plasmon resonance signal. On a UV-Vis spectrophotometer, a high concentration of penicilliamine-Au complex was determined, indicating that penicillamine was already well combined with Au colloid. The method, using the combination of Au colloid with penicillamine, proved to detect penicillin. 相似文献
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表面等离子体共振生物传感器连续检测莱克多巴胺 总被引:6,自引:0,他引:6
利用表面等离子体共振生物传感器对莱克多巴胺抗体与固定在芯片表面的莱克多巴胺衍生物的相互作用进行了分析,解离常数为2.56×10-6s-1。根据一定范围内相对响应值和时间近似呈线性关系的动力学特性,建立了连续检测的方法,从而简化了实验步骤,有利于提高芯片的使用寿命。检测莱克多巴胺采用抑制法,将莱克多巴胺衍生物固定在芯片的表面,莱克多巴胺抗体与样品混合后流过芯片的表面,所得相对响应值与样品中莱克多巴胺的浓度成反比。单个样品的检测时间设定为15min,对应的检出限小于4μg/L。 相似文献
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《Analytical letters》2012,45(15):2210-2220
In this work, a surface plasmon resonance sensor system was designed and implemented for determination of nucleic acids in unpurified samples. First, through blocking non-specific interaction sites on the sensor surface to reduce non-specific adsorption from unpurified sample matrix, it was determined that at the optimal BSA concentration of 100 µg/ml the non-specific interaction can be reduced by 50%, although improvement for direct detection of nucleic acids in unpurified sample is required. Second, bearing nonspecific adsorption onto gold films, nucleic acids adsorbed on sensor surface in unpurified sample matrix were detected through a secondary hybridization approach. Using DNA-lined AuNPs shows the new SPR sensor can be applied for the determination of target ssDNA with a detection range of 0.1–10 µM for targets in purified and 1–10 µM in unpurified samples, respectively. Results imply that the new SPR sensor system is promising for specific and convenient analysis of nucleic acids directly in unpurified samples. Development of the new SPR sensor technique can have applications in fast field diagnostics and monitoring. 相似文献
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YANG Mengchao JIN Xinyu YUAN Maokai LIU Fan LANG Yicong WU Zhongyu XIANG Liancheng ZHOU Chao OUYANG Bo JIN Wei MU Ying 《高等学校化学研究》2014,30(2):211-215
A laboratory-made tumor cell detection device was fabricated based on both surface plasmon resonance imaging(SPRi) and image processing.In this device,a gravity-induced flow injection chip(gFIC) was exploited to replace a pump.Also two charge coupled devices(CCDs) were used to detect HepG2 cells by SPRi and image processing,respectively.The results of two CCDs are associated.Protein A was used to modify the sensing surface.The inlet angle was carefully adjusted for the device to get an enhanced image.In the test,the contrast among cell solutions at different concentrations can be easily distinguished.The other CCD using image processing can tell false-positive in some degree.This detection is label-free,real time,and precise. 相似文献
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建立了一种基于表面等离子体共振(SPR)技术测定畜禽肉中泰乐菌素残留的新方法.用传感芯片(Sensor chips CM5),以25%ACN-0.25 moL/L NaOH为再生溶液,HBS-EP为缓冲溶液,流速为40 μL/min,运用表面等离子体共振(SPR)技术测定畜禽肉中泰乐菌素的含量,结果表明泰乐菌素在32 ng/mL范围内,在Sensor chips CM5表面所产生的相对共振强度与质量浓度有良好的响应关系,平均回收率为80.0%~99.5%,RSD为2.0%~4.7%,检出限为0.03 ng/mL.该方法简便、灵敏,可以为产品的安全与质量控制提供快速分析方法. 相似文献
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基于表面等离子体子共振的B因子传感器 总被引:2,自引:0,他引:2
以分辨率较高的一米光栅单色仪为分光系统,光电倍增管为检测系统,改进了自行组装的表面等离子体子共振(SPR)传感装置,提高了仪器的检测能力约50倍,对于发展改变波长模式的SPR传感器具有重要意义。以对金和抗体均有较强吸附作用的葡萄球菌A蛋白为基底膜,观测了人的B因子抗体和抗原之间免疫反应的动力学过程,并研究了B因子的定量测定。结果表明,B因子抗原的浓度在0.02~5μg/mL范围内与信号的响应值呈线性关系。该传感器灵敏度高,选择性和重现性均好。 相似文献
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利用表面等离子体共振技术进行TNF免疫复合物的动力学分析 总被引:1,自引:0,他引:1
表面等离子体共振 ( Surface plasmon resonance,SPR)技术是近年来发展起来的测定分子间相互作用的技术 ,应用最广泛的是 Pharmacia公司发展的生物分子相互作用实时分析 ( Biomolecular interactionanalysis,BIA)技术 [1~ 3] .它可以实时、原位地测定生物大分子间的相互作用 ,而反应物无需标记 ,可以测定反应的动力学常数 ,这对于进行反应的动力学分析和机理研究 ,以及进行性质鉴定和筛选应用具有非常重要的意义 [4~ 8] .肿瘤坏死因子 ( Tumor necrosis factor,TNF)是临床上用于肿瘤治疗的非常有效的细胞因子类药物 [9] .本文报道了… 相似文献
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表面等离子体子共振技术实时研究蛋白质在修饰表面的静电吸附行为 总被引:4,自引:0,他引:4
研究蛋白质在固相表面的静电吸附特性,进而控制蛋白质在修饰表面的静电吸附尤为重要,表面等离子体子共振可以检测金属表面吸附物质厚度和折射率的变化^[1]。这种技术已在研究生物分子相互作用^[2]和考察自组装单层的形成^[3]及蛋白质在固体表面吸附行为^[9-11]等方面得到广泛的应用。对蛋白质在固体表面吸附行为的研究多为考察不同的蛋白质在不同的修饰表面的吸附行为。然而,对蛋白质在修饰表面静电吸附的本质影响因素的研究却少有报道^[4]。本文使用表面等离子体子共振技术实时研究了蛋白质在甲羧基化葡聚糖修饰表面的静电吸附与溶液pH值及离子强度的依赖关系。 相似文献
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This paper reports results obtained when comparing an electrochemical enzyme immunosensor and a surface plasmon resonance (SPR) based immunosensor on the same gold surface installed in an electrochemical SPR flow cell. Simultaneous electrochemical and SPR measurements were performed on a gold surface modified with multilayers of poly‐L ‐lysine and poly‐styrenesulfonate assembled with the layer‐by‐layer method. First, we obtained the SPR response induced by the formation of an immunocomplex from the shift in the SPR angle by injecting an anti tumor necrosis factor‐α antibody solution labeled with alkaline phosphatase into the flow cell containing the multilayer modified with tumor necrosis factor‐α. Then we compared this SPR result with that obtained for the electrochemical oxidation current of p‐aminophenol catalyzed by alkaline phosphatase from p‐aminophenolphosphate on the same gold film. We compared the two immunosensor responses obtained using the different measurement principles and found that there was a high correlation efficient of 0.973 between them. This was because we were able to immobilize the immunoreagents with good stability and without losing the transport of the enzyme product in the multilayer whose thickness we easily controlled with nanometer scale accuracy. We also report that the detection limit of our electrochemical immunosensor after optimization was around 100 pg/mL (0.4 pM), which is one of the lowest values yet reported for an electrochemical immunosensor. 相似文献
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Chia‐Chen Chang Tsung‐Liang Chuang Da‐Shin Wang Ching‐Ho Wang Chii‐Wann Lin 《中国化学会会志》2013,60(12):1449-1456
Protein A and protein G are extremely useful molecules for the immobilization of antibodies. However, there are limited comparative reports available to evaluate their immobilization performance for use as biosensors. In this study, a comparative analysis was made of approaches that use protein A and protein G for avian leukosis virus detection. The antibody‐protein binding affinities were determined using surface plasmon resonance (SPR) analysis. The immobilization efficiency was obtained by calculating the number of the protein molecular binding sites. The positive influence of sensor response on antigen detection indicates that the amount of immobilized antibody plays a major role in the extent of immobilization. Moreover, the biosensors constructed using both proteins were found to be regenerative. The SPR results from this study suggest that the surfaces of protein G provide a better equilibrium constant and binding efficacy for immobilized antibodies, resulting in enhanced antigen detection. 相似文献
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构建了一种选择性检测睾酮素的分子印迹表面等离子体共振( Surface plasmon resonance, SPR)传感器。采用紫外光引发表面接枝技术,在固定引发转移终止剂的SPR芯片表面制备了以睾酮素为模板分子,甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂的分子印迹膜( Molecularly imprinted film, MIF)。利用SPR在目标共振角处现场监测使MIF聚合成膜过程更易控。偏振调制-红外反射吸收光谱表征证明MIF接枝成功。原子力显微镜结果显示,MIF 表面均匀散布着纳米尺寸的孔穴。利用 SPR 对2.5×10-16~2.5×10-6 mol/L睾酮素进行吸附检测,检出限低至2.5×10-16 mol/L,对低浓度和高浓度的睾酮素吸附分段进行线性拟合,得到拟合线性方程分别为y=19.69+1.21x(R2=0.9913)和y=11.5+0.45x(R2=0.9895);睾酮素类似物雌二醇、雌三醇和黄体酮的吸附实验结果显示,此印迹膜对模板分子有很好的选择性;5次重复洗脱吸附后,MIF仍保持较好的传感性能,说明此传感器具有较高的稳定性和重复利用性;在人工尿液样品中测得的睾酮素回收率为85.2%~92.8%,说明此传感器可以用于实际样品测定。 相似文献
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In this study, surface plasmon resonance(SPR) for monitoring 17β-eatradiol( E2 ) was developed. The small molecule E2 was immobilized on a CM5 sensor chip for an indirect competitive immunoassay to detect F2. The SPR response based on the antigen-antibody reaction was measured by injecting the sample solution into the flow system.The limitation of detection was 0.445 μg/L. The developed SPE-SPR system was applied to analyze the seawater samples. Recovery of E2 was 91.6%-93.1%. Relative standard deviations(RSD) for the E2 assay were between 10.9%-15.1% ( n = 3). The range of determination of E2 samples from the sewage in the coastal marine environment was between ND(lower than detection limit) and ca. 11.78 ng/L. 相似文献
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LUO Zha LIU Hao SHEN Wei SHI Kun CHEN Ankang SHENG Libo SUI Yongming ZOU Bo 《高等学校化学研究》2022,38(3):843-846
We used a diamond anvil cell(DAC) to control the deformation of synthesized copper nanorods and silver nanoparticles. And we measured the surface plasmon resonance of copper nanorods and silver nanoparticles, which exhibit redshifts or blueshifts. The surface plasmon resonance shows an abnormal blue shift for both copper nanorods and silver nanoparticles. The solvents of copper nanorods and silver nanoparticles are n-hexane and water, where the pressure loads include quasi-hydrostatic and non-hydrostatic. 相似文献
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基于表面等离子体子共振成像技术研究苏拉明和顺铂对肝癌细胞生长的抑制作用简 总被引:1,自引:0,他引:1
基于表面等离子体子共振成像(SPRi)技术提出了一种实时、非标记的新型抗癌药物药效评估方法. 以聚二甲基硅氧烷(PDMS)为材料,制作了包含微柱结构的微流控芯片作为流通反应池,配合自行设计组装的SPRi生物传感器完成肿瘤细胞的特异性捕获及检测,研究了苏拉明和顺铂对肝癌细胞HepG2的生长抑制作用. 同时引入辅助验证实验,即采用常规八肽胆囊收缩素(简称CCK-8)法测定上述药物对肝癌细胞增殖的抑制作用. SPRi检测结果表明,苏拉明和顺铂能抑制肿瘤细胞HepG2增殖并呈现剂量、时间依赖关系. 相似文献
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Li Hua CHEN 《中国化学快报》2006,17(12):1619-1622
Recently, surface plasmon resonance (SPR) become more and more popular without the need of the label technology1-3. However, sometimes, a number of experimental artifacts complicate the final biosensor analysis4-7. The utilization of a reference surface c… 相似文献
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用表面等离子体子共振传感器测定人心肌肌钙蛋白I的研究 总被引:1,自引:0,他引:1
采用自组装表面等离子体子共振(SPR)传感装置,固定入射角,以波长为变量,以CCD为检测系统,用对金和抗体均有较强吸附作用的葡萄球菌A蛋白作为基底膜,观测了人心肌肌钙蛋白I的抗体和抗原之间免疫反应的动力学过程,并进行了人心肌肌钙蛋白I的定量测定.结果表明,人心肌肌钙蛋白I的浓度在5.0~50μg/L范围内与传感器的响应值呈线性关系. 相似文献