Automated Determination of Captopril by Flow and Sequential Injection Analysis: A Review

The present study intends to present a review of automated methods for the determination of Captopril—an angiotensin converting enzyme (ACE) inhibitor—using flow or sequential injection analysis. The review covers a range of more than fifteen years of published research on this topic (1993–today). The methods are classified according to the detection systems in three categories, namely spectrophotometric, chemiluminescence, and electroanalytical. The principles and main analytical figures of merit of the reported studies are presented and discussed.     

Sequential Injection Analysis Hyphenated with Other Flow Techniques: A Review

In the last two decades, sequential injection analysis (SIA) became an important automation tool in several analytical fields as it provided advantages in terms of versatility, robustness, and consumption of samples and reagents.

The noteworthy versatility of an SIA selection valve allowed its association with several units, including devices typically associated with other flow techniques, resulting in generally better analytical performance.

This review discusses the hyphenization of SIA with other flow approaches outlining its advantages and motivations, which are related mainly with sample manipulation and solutions management. The future trends and perspectives in this field are also addressed.     

顺序注射分析及其应用

本文回顾了顺序注射分析的发展过程,对其概念,理论、技术及应用进行了综述,上用文献七十会篇。     

The Use of Chelating Solid Phase Materials in Flow Injection Systems: A Review

Flow injection analyses facilitate faster, inexpensive, and more environmentally friendly sample-preparation techniques than many traditional “wet chemistry” methods, and the use of flow injection has, therefore, expanded significantly in recent years. This review focuses on chelating solid phase materials, which have been used in flow injection analyses and also describes important information, such as type of chelating groups, the type of stationary phases, and their efficiency at pre-concentrating metal ions. Discussions of the improvement of the sensitivity and of improving limit of detection, as well as the efficiency with which concomitant ions are removed for the flow injection methods using chelating solid phase materials, are also made.     

流动注射-可更新表面技术进展

概述了流动注射－可更新表面技术的进展。介绍了该技术的原理、仪器发展及其在免疫分析、生物配位作用检测、细胞功能检测、分离与预浓集等领域的应用。参考文献27篇。     

流动注射分析系统中的化学动力学研究

运用对流－扩散模型研究了流动注射分析系统中的化学动力学，基于无化学反应时试样分散的浓度分布公式，运用数学归纳法导出了在一定条件下一级和二级反应的产物浓度分布公式，所得解析解便于计算机模拟产物生成曲线。解释了双峰的产生，并讨论了相关的动力学信息。     

流动注射化学发光法测定制剂和血浆中的盐酸多巴胺

在碱性介质中,盐酸多巴胺对鲁米诺-KIO4化学发光体系有明显的增强作用,据此,结合流动注射技术,建立了微量快速测定盐酸多巴胺的流动注射化学发光分析法。该法线性范围为0．1—100μg∥L,检出限0．02μg／L（3σ）;对1．0μg/L盐酸多巴胺11次平行测定,其相对标准偏差为2．9％。此法已成功地用于注射剂及血浆中盐酸多巴胺的分析。     

流动注射分析血清中葡萄糖的电化学检测池

测定血清中葡萄糖含量是临床检验的重要项目之一。何立千曾对各种血糖测定方法做过综述。葡萄糖氧化酶-4-氨基安替比林-苯酚光度法(酶制剂法)虽能较好地测定血清中葡萄糖的含量,但成本较高,耗时长。因此有必要研究一种快速、廉价、稳定的分析方法。人们考虑到酶的专属性,对葡萄糖氧化酶电极做了大量工作,然而,这种电极的寿命仍不理想。     

Flow Injection Analysis System Using Magnetic Beads,Screen Printed Electrodes and Magnets

Here is presented a Flow Injection Analysis (FIA) system using a flow cell with an integrated magnet, applied to biotin determination. The mixture of magnetic beads modified with streptavidin (Strep‐MB), biotin and B‐HRP is left 15 minutes under stirring and then a washing step is performed in an automatic way thanks to the external magnets coupled in the FIA system. After the immobilization of the MBs on the surface of the electrode, 3,3′,5,5′‐Tetramethylbenzidine (TMB) is injected. The linear range obtained is between 0.5 to 10 pM of biotin and the sensitivity is 85 nA/pM.     

芯片式流通池顺序注射化学发光法测定水中的亚硝酸根

微流控芯片(Microfluidic chips)是微全分析系统(μTAS)研究中最为活跃的领域,在仪器微型化方面展现出很多的优点^[1].化学发光由于其自身的特性在微芯片检测中应用逐渐增多^[2,3].     

流动注射分析中提高线性范围的一种方法

经证明，在流动注射分析中使用光度检测器和进样体积一定时，下式成立：ｃ＝ａ＋ｂｌｎ（１－ｈ／ｈ０）。式中ｃ为试样浓度，ａ和ｂ为与实验条件有关的系数，ｈ和ｈ０分别为试样峰和定态时的峰高。ｈ０几乎不随浓度增加而变化。应用此式可提高测定的线性范围，进行较高浓度试样的测定。     

流动注射分析用新型钆离子电极

功能高分子为活性材料,加热压片,制成新型钆电极。其性能较PVC式钆离子选择电极为优。取钆电极膜片制成流通式钆电极,与流动注射仪联用,测得电极斜率59mV,与静态响应相似。每次进样500μl,测定频率每小时60～100次。操作简单,重现性好。     

微酶电极的研制及在流动注射分析中的应用

利用铂化电极的大表面吸附葡萄糖氧化酶（简称ＧＯＤ）构建了一种具有良好操作性能的微酶电极，在０～１０ｍｍｏｌ／Ｌ葡萄糖浓度具有很好线性，相关系数γ＝０．９９８，响应时间短（＜２０ｓ），精度高。利用该电极组成三电极流动注射分析系统，这种系统同样有较好的线性范围，分析频率达４５ｈ＾－１，使用寿命在一周以上。     

A Sensitive Photoinduced Chemiluminescence Method for the Determination of Riboflavin with Flow Injection Analysis

ABSTRACT

A sensitive chemiluminescence (CL) induced by light for the determination of riboflavin with flow injection analysis is developed. Riboflavin shows a strong enhancement effect on the CL reaction of luminol oxidized by periodate after the photochemical reaction of riboflavin in alkaline solution, and the enhancing effect of riboflavin disappears under dark conditions. Oxygen can further improve the enhancing effect of the riboflavin in this CL system, and the effects were also investigated. And based on this phenomenon, a very sensitive method of various surfactants, β-cyclodextrin and organic solvents on this CL system for the determination of riboflavin was described. The range of the linear response for riboflavin using this method is ca. 2.4×10^?8 ~ 1.0 × 10^?6 mol/L, and the detection limit (S/N = 3) is l.0×10^?8 mol/L. The applicability of the method was demonstrated by the determination of riboflavin in pharmaceutical preparation.     

乳酸脱氢酶反应器用于流动注射分析测定人体液中的L-乳酸

本文用[3-(2-氨乙基)氨丙基]三乙氧基硅烷试剂胺化一定孔径大小的载体,用戊二醛作偶联剂,将乳酸脱氢酶固定于其上,装入柱内,联结于流动体系中。L-乳酸的浓度在0.1～4.4mmol/L呈线性,检测限为0.4nmol(s/N=4),相对标准偏差≤1.2％,回收率在96％～103％。每小时进样35次。测定了固定化LDH的表观米氏常数及人血清和尿中的L-乳酸。     

色谱检测器理论在流动注射分析中应用的初步研究

本文运用色谱检测器理论，由峰面积与载流流速的乘积是否为一常数可判断某一化学反应是否为快速反应。     

间氯偶氮安替比林流动注射分析测定药物及水中钙

基于间氯偶氮安替比林可与钙在碱性介质中形成灵敏的兰色络合物这一显色反应，本文在自行组装的带微型计算机的流动注射分析仪上，以分光光度计作检测器，建立了测定微量钙的流动注射分光光度法。最佳显色反应条件：０．０８ｍｏｌ／Ｌ ＮａＯＨ－０．０１２％显色剂２．０％三乙醇胺溶液，测定波长为６３０ｎｍ。以三乙醇胺为掩蔽剂，方法选择性较高。钙的线性范围为１．０￣１５．０μｇ／ｍｌ，检测限０．５μｇ／ｍｌ，进样频率