指血中Zn、Fe、Ca、Mg的分析方法研究

提出了一种直接测定血中Zn、Fe、Ca、Mg的原子吸收测定方法。本法用一定量的基体释放剂，取少量血样于小体积中，直接在原子吸收仪器上进行测定。对以往的血液中微量元素测定，尤其对采集体积少的标本测定方法作了改进。     

严重偏食儿童血中金属元素改变的研究

为了解严重偏食儿童血中金属元素的变化，用火焰原子吸收法对８０名严重偏食儿童轿中铜、锌、铁、钙、镁进行了测定。结果表明，严重偏食儿童血中铜、锌、铁、钙、镁含量均明显低于正常对照组（Ｐ〈０．０１），会影响儿童的生长发育，应予纠正。     

基体改进效应用于石墨炉原子吸收测定生物和环境样品中痕量金属元素

本文综述了基体改进技术在石墨炉原子吸收分析中的应用,提出了基体改进效应用于石墨炉原子吸收测定血中铅、土壤、粮食和蔬菜中的碲、水中价态锑的新方法。     

哺乳妇女血、乳中三种元素含量检测

研究了哺乳期母体内血、乳中锌、铁、钙三种元素含量的检测。探讨了用一定量的基体释放剂，取少量血液、乳液经大倍数稀释，直接于原子吸收仪上进行测定。此法快速、准确、实用。     

磷光寿命的研究及生物体内色氨酸的测定

研究了磷光寿命的特性及ｐＨ、溶剂极性、碘、铅、钡、铊等重原子对磷光寿命的影响。应用磷光寿命变化法，分析生物样品中色氨酸含量。小鼠体内，血、脑、心、肺、肝、脾、肾中游离色氨酸分别为８．９μｇ／ｍＬ，１７．３，５１．８，２９．１，２９．９，４６，６８．８μｇ／ｇ。其中，组织血脑中色氨酸含量与文献报道一致。     

类风湿关节炎患者血浆置换治疗前后微量元素铜的变化

应用原子吸收分光光度法，对１５例类风湿关节炎（ＲＡ）患者血浆置换前后微量元素铜的变化进行了测定。结果表明，血浆置换后患者的血铜水平显著下降，提示血浆置换治疗ＲＡ存在铜元素的机制。     

血橙中微量元素的测定

采用电感耦合等离子体原子发射光谱仪(ICP-AES),对血橙果肉汁、皮、籽中10种微量元素进行了测定。结果表明,血橙果肉汁、皮、籽中含有Ca、Mg、Fe、Zn、Mn、Cu、Ni等丰富的人体必需微量元素,且血橙果皮、籽中的微量元素含量普遍高于果肉汁中的微量元素的含量,这为血橙药效功能的进一步研究和资源综合开发利用提供一定的科学依据。     

南昌市城区416名儿童锌缺乏状况调查

为了解南昌市城区儿童锌缺乏状况，采集手指尖末梢血20μL用石墨炉原子吸收分光光度法测定全血锌浓度。以血锌值5.00μg/mL为正常下限。结果表明，该儿童人群血锌值呈非正态分布，样本中位数为5.659μg/mL，四分位数间距为Q3－Q1＝1．927。416名2－8岁儿童中男240人，女176人。锌缺乏人数134人，锌缺乏患病率35．80％。不同性别间血锌值无显著差异，而不同年龄间血锌值有显著差异，但未表现出与年龄呈正相关关系。提示南昌市城区儿童锌缺乏的发生率较高，应引起人们足够的重视。     

火焰原子吸收光谱法测定小儿生血糖浆中硫酸亚铁

建立火焰原子吸收光谱法测定小儿生血糖浆中硫酸亚铁的分析方法.采用火焰原子吸收光谱法测定小儿生血糖浆中铁的浓度,根据铁的浓度换算成硫酸亚铁的浓度.火焰燃烧类型为空气-乙炔,测定波长为248.3 nm,测定时间为4.0 s,燃气流量为0.9 L/min,燃烧器高度为7.2 mm,氘灯作为背景校正.结果表明,小儿生血糖浆中铁...     

气相色谱－原子发射光谱联用仪测定18种安眠镇静药的元素比例及其在中毒病人全血样品中的应用

采用气相色谱－原子发射光谱联用仪（ＧＣ－ＡＥＤ）测定了１８种安眠镇静药的元素组成和元素比例。根据气相色浴－质谱（ＧＣ－ＭＳ）测定的分子量数据推测出它们的分子式，结果与实际值一致。以元素组成、元素比例和保留时间为判据，对一例中毒患者全血样品进行了定性分析，发现血中含有苯巴比妥。     

Determination of Nickel,Manganese, Copper and Zinc in Blood Serum by Atomic Absorption Spectrometry after Deproteinization by Microwave Irradiation

ABSTRACT

Deproteinization of serum was performed by microwave irradiation combined with a small volume of diluted trichloracetic acid. The procedure reduced the protein level of the samples to less than 99% of the total with a small dilution factor (1+1) and allowed the determination of nickel and manganese by electrothermal atomic absorption spectrometry and copper and zinc by flame atomic absorption spectrometry directly without modifiers or matrix interferences. As metallic ions are normally bonded to serum proteins they must be released during protein precipitation. Spiked serum samples were submitted, before the deproteinization, to an incubation treatment to bond the added ions to the proteins. To check the efficiency of the incubation time for each ion, serum samples were ultrafiltered at set time intervals and the metals determined in the ultrafiltrate. The proposed method was compared with the common deproteinization by acids for the separation of the proteins. The reduction of proteins allowed a small dilution of the sample and the use of faster temperature programmes for the determination of nickel and manganese by electrothermal atomic absorption spectrometry and the aspiration of samples more similar to aqueous standards for copper and zinc determination by flame atomic absorption spectrometry. Recoveries from spiked, incubated and deproteinized samples compared to only diluted samples show that the method can satisfactorily be used for atomic absorption spectrometric determination of these elements.     

海水中铜锌铅镉的萃取-反萃取法同时提取-原子吸收光谱法测定

采用萃取-反萃取法同时提取出海水中Cu、Zn、Pb、Cd,Cu、Pb、Cd石墨炉法测定,Zn用火焰法测定.对样品的前处理方法和最佳仪器条件进行了研究,并通过加标实验验证了方法的可靠性.结果表明:萃取-反萃取法能完全满足海水中Cu、Zn、Pb、Cd的前处理要求,可节省一半前处理时间,有溶液稳定、环保、节省试剂等优点.     

原子吸收法测定血清中的无机磷和钙

本文叙述了用原子吸收法测定同一个少量血清样品中的无机磷和钙。血清用高氨酸沉淀蛋白后,加过量钼酸铵。在pH0.4～1.6范围内,用乙酸丁酯萃取生成的微量磷钼酸,与过量的钼酸铵分离。水相测钙,有机相用稀氨水反萃后测钼,间接分析磷。     

电化学微流池富集-电热原子吸收法测定海水中(超)痕量镉

建立了电化学微流池与电热原子吸收光谱联用技术对(超)痕量镉进行测定的方法。利用自制电化学微流池对(超)痕量镉富集,后经反向电压和酸液洗脱,洗脱液由电热原子吸收光谱仪进行测定。优化了测定条件,实验结果表明,在0.05~0.80μg/L范围内成线性关系,相关系数为0.9912,检出限(3σ)达到0.004μg/L,富集倍率为11。将该方法应用到海水中(超)痕量镉的测定,相对标准偏差在2.2%~3.4%(n=3),回收率在86.0%~95.5%。     

贵金属元素铂、铑、钯的分析进展

从光度法、电化学法、原子吸收法、中子活化法、电感耦合等离子体与原子发射光谱、质谱联用技术等各种方法,对国内外近几年来贵金属元素铂、铑、钯的测定方法进行综合评述,引用文献168篇。     

Comparison of a graphite tube micromethod for the determination of serum iron and total iron-binding capacity with spectrophotometric techniques

A rapid micromethod by flameless atomic absorption spectrometry is presented for the determination of total iron in serum or plasma. A clear distinction is drawn between “total iron in serum” and “serum iron”. The results for serum iron are compared with existing spectrophotometric methods. Comment is made on diurnal variation in serum iron concentration.     

火焰原子吸收光谱法测定茯苓中微量元素

本文确定了各微量元素火焰原子吸收光谱仪的工作条件,建立了各微量元素线性回归方程.应用火焰原子吸收光谱法测定了天然茯苓和液体发酵茯苓中微量元素含量.研究结果表明二者微量元素含量存在一定的差异,探讨了产生上述差异的原因.     

石墨炉原子吸收法直接测定血清中硒,砷

选用Ｐｄ－Ｎｉ－Ｔｒｉｔｏｎ Ｘ－１００为基体改进剂，用石墨炉原子吸收法直接血清中砷、硒。该法具有操作简单、快速、灵敏度高的特点，为临床测定血清中砷、硒提供了一种新方法。     

Determination of aluminum in small samples of serum and biopsy samples of bone and soft tissues

Rapid micromethods for the determination of aluminum in serum and biopsy material by flameless atomic absorption spectrometry are presented. Matrix effects are emphasized and are overcome by the method of additions.     

The determination of trace metals in human fluids and tissues : Part 1. Estimation of “Normal Values” for Copper,Zinc, Cadmium and Manganese in Blood Serum and Liver Tissue

The procedures for the determination of copper, zinc, cadmium and manganese in human blood serum and liver tissue with use of flame and electrothermal atomic absorption spectrometry are presented and discussed. The procedures were applied for the estimation of “normal” levels of these elements in human serum and liver tissue.