共查询到20条相似文献,搜索用时 15 毫秒
1.
The interactions between three types of polyamidoamine dendrimers (with anionic, cationic, and neutral charge on a surface) and fluorescent dye 1-anilinonaphthalene-8-sulfonate (ANS) were studied. Double fluorimetric titration method was employed to estimate a binding constant and the number of binding centers. As fluorescent probes can serve as models of toxin molecules, dendrimers, and human serum albumin (HSA) abilities to bind ANS were compared. In the presence of HSA and dendrimers, ANS located both in HSA and in dendrimers, but the interactions between ANS and HSA were stronger. 相似文献
2.
Jokiel M Shcharbin D Janiszewska J Urbanczyk-Lipkowska Z Bryszewska M 《Journal of fluorescence》2007,17(1):73-79
The interactions between polycationic poly-lysine dendrimers and hydrophobic fluorescent probes (anionic ANS and neutral Prodan)
were studied. R121 and R131 dendrimers were not able to interact with anionic and neutral hydrophobic groups. R124 was able
to interact with neutral and anionic hydrophobic fluorescent probes, however mainly through hydrophobic forces. Dendrimers
R155 and R169 showed the maximal effects. The strongest interactions observed for R169 can be explained by intramolecular
folding (stacking) of its two L-proline residues. Using double fluorescence titration technique for ANS probe allowed to receive
such constant of binding and the number of binding centers: for R121, 1.8·103 (mol/l)−1 and 1.07; for R124, 12.1·103 (mol/l)−1 and 0.48; for R131, 4.7·103 (mol/l)−1 and 0.48; for R155, 9.2·103 (mol/l)−1 and 1.36; for R169, 39.6·103 (mol/l)−1 and 0.97. Thus, neutral and anionic hydrophobic probes can be used for the fast preliminary screening of binding properties
of newly synthesized polycationic dendrimers. 相似文献
3.
本文利用多种核磁共振(NMR)技术研究了聚酰胺-胺树状大分子(PAMAM)与5-氟尿嘧啶(5-FU)在水溶液中的相互作用.1H NMR化学位移滴定结果表明5-FU分子结合在PAMAM分子表面,13C NMR化学位移滴定分析结果表明5-FU分子通过氢键或疏水间相互作用进入到PAMAM的内部疏水空腔.1H核和19F核自旋-晶格弛豫时间(T1)的测定结果也说明了二者之间存在相互作用.NOESY实验进一步验证了5-FU分子进入到PAMAM分子的内部空腔.本研究为研究树状大分子和药物小分子的相互作用提供了系统的NMR分析方法. 相似文献
4.
用荧光分光光度法研究了Zn2+,Mn2+,Cd2+,Na+,K+,Ag+,Cu2+和Pb2+等金属离子修饰的ZnS/PAMAM树形分子纳米复合材料的荧光发射性能。结果表明:不同金属离子修饰效果不同。Zn2+,Mn2+和Cd2+修饰后,ZnS/PAMAM树形分子纳米复合材料的荧光发射强度有不同程度提高;Ag+,Cu2+和Pb2+的修饰对荧光有不同程度的猝灭作用;而Na+和K+的修饰对荧光发射无明显影响。与修饰前相比,Cd2+离子修饰的ZnS/PAMAM树形分子纳米复合材料标记的潜指纹发射的蓝色荧光更加明亮,与背景反差更加明显。这对提高潜指纹的显现精度和准确率有很好的借鉴价值。 相似文献
5.
在Zn2+离子与聚酰胺-胺(PAMAM)树形分子配位的基础上,制备了稳定的PAMAM树形分子包覆的ZnS量子点(quantum dots,QDs),并用紫外-可见吸收光谱(UV-Vis)和荧光发射光谱进行了表征。结果表明,Zn2+离子能与PAMAM树形分子发生配位络合作用,且饱和配位时间为6h;在波长365nm紫外光的激发下,PAMAM树形分子包覆的ZnS量子点发射出明亮的蓝色荧光,荧光发射峰约位于450nm。最后,将得到的PAMAM树形分子包覆的ZnS量子点纳米复合材料应用于锡纸上潜指纹的荧光标记成像研究,发现指纹可以被清晰识别,呈现明亮的蓝色荧光指纹。 相似文献
6.
采用荧光发射法研究了不同Cd2+与树形分子的摩尔比(负载比)条件下硫化镉(CdS)/聚酰胺-胺(RAMAM)树形分子纳米复合材料(NCs)水溶液的荧光性能,并探索了其荧光产生的机理.结果表明:在波长330 nm光的激发下,CdS/G4.0-NH2PAMAM NCs的荧光发射光谱中出现了2个峰,一个是PAMAM树形分子的... 相似文献
7.
In recent years, one prevalent competitive fluorescent probe, N-phenyl-1-naphthylamine (1-NPN), was frequently utilized to measure the binding affinity of entomic odorant binding proteins (OBPs) with diverse plant volatiles or pheromones. Nevertheless, the details and model of the binding interaction are still largely unknown, although it is vital to investigate the physiological function of OBPs. Here we studied the binding interaction between 1-NPN and OBP2, a recombinant OBP from eastern honeybee, Apis cerana, by the combination of fluorescence quenching spectra, synchronous fluorescence spectra, ultraviolet spectra, circular dichroism spectra, and molecular docking. The Stern–Volmer curve of the fluorescence quenching of OBP2 by 1-NPN indicated it was a static quenching mechanism, and the binding constants and binding number were determined, respectively. Based on the Förster theory of nonradiation energy transfer (FRET), the binding distance was calculated, and the intrinsic fluorescent energy was predicted to transfer from the donor OBP2 to the acceptor 1-NPN. Synchronous fluorescence spectra and circular dichroism spectra were used to investigate the conformational change in binding progress. The thermodynamic parameters showed that the interaction was mainly driven by hydrophobic force, which was validated by the molecular docking; meanwhile, the binding mode was revealed and one hydrogen bond was found between the nitrogen atom of 1-NPN and Glu29 of OBP2. 相似文献
8.
Determination of binding parameters such as the number of ligands and the respective binding constants require a considerable number of experiments to be performed. These involve accurate determination of either free and/or bound ligand concentration irrespective of the measurement technique applied. Then, an appropriate theoretical model is used to fit the experimental data, and to extract the binding parameters. In this work, the interaction between bovine serum albumin (BSA) and 1-anilino-8-naphthalene sulphonate (ANS) is revisited. Using steady state fluorescence spectroscopy, the binding isotherm of BSA/ANS was obtained applying the Halfman-Nishida approach. The binding parameters, site number, and binding site association constants, were determined from the stoichiometric Adair model and Job's plot. The binding parameters obtained were then correlated to the distance of the respective binding site to the tryptophan residues using the energy transfer technique. This approach, that uses both tryptophans independently from each other, is presented as a tool to help understand the binding mechanism of the albumin fluorescent complex. The results show that ANS molecules bind to BSA in up to five different binding sites. Energy transfer from the tryptophan residues to the BSA/ANS complex shows that the four highest affinity binding sites (>10(4) M(-1)) are located at a reasonably close distance (18-27 A) to at least one of two tryptophan residues, while the lowest affinity binding site (approximately 10(4) M(-1)) is located over 34 A away from the both tryptophans. 相似文献
9.
In the presented work studies of the interaction mode of monomer and two homodimer benzothiazole styryl dyes containing spermine-like
linkage/tail group with the double stranded (ds) DNA are reported. For these dyes, equilibrium constant of dye binding to
DNA (K
b), as well as the number of dsDNA base pairs occupied by one bound dye molecule (n) were determined. The data obtained show that the presence of spermine-like group containing quaternary nitrogen (Bos-5)
results in increase of K
b value as compared to this of unsubstituted analogue (Sbt). Besides, for the dimer dyes containing benzothiazole styryl chromophores,
the K
b value is either five times higher (DBos-13) or almost the same (DBsu-10) as compared to this of corresponding monomer Sbt,
depending on the position in the benzothiazole ring where the linker is attached. Moreover, the n values for both dimers are significantly different as well, pointing to the bis-intercalative binding mechanism for DBos-13
and for the groove-binding one for DBsu-10. The conclusion about the dimer dyes-dsDNA binding mechanisms is also supported
by the study of the fluorescent response of these dyes on the presence of AT- and GC-containing polynucleotides. 相似文献
10.
利用荧光光谱法,紫外可见光谱法,红外光谱法,圆二色谱法和等温滴定量热法等手段,对抗癌药物盐酸阿霉素 (DOX) 与DNA的作用过程进行研究,测得了它们的结合常数Ka、结合位点数n、反应焓变ΔH、熵变ΔS及ΔG,且在结合过程中,B型DNA的螺旋结构在一定程度发生改变。荧光光谱的数据显示出显著的猝灭效应, 表明DNA是一个DOX荧光的很好的猝灭剂。红外光谱表明阳离子DOX+通过静电吸引与 DNA 的磷酸基团相互作用,且DOX的碳氢链通过疏水缔合与DNA作用。ITC测定了DOX和DNA相互作用的焓变和熵变,表明DOX的烃链和DNA的碱基之间的疏水性相互作用提供了结合的驱动力。 相似文献
11.
Dietmar Eberbeck Frank Wiekhorst Uwe Steinhoff Kay Oliver Schwarz Andreas Kummrow Martin Kammel Jörg Neukammer Lutz Trahms 《Journal of magnetism and magnetic materials》2009,321(10):1617-1620
The binding of monoclonal antibodies labelled with magnetic nanoparticles to CD61 surface proteins expressed by platelets in whole blood samples was measured by magnetorelaxometry. This technique is sensitive to immobilization of the magnetic labels upon binding. Control experiments with previous saturation of the epitopes on the platelet surfaces demonstrated the specificity of the binding. The kinetics of the antibody antigen reaction is accessible with a temporal resolution of 12 s. The minimal detectable platelet concentration is about 2000 μL−1 (sample volume 150 μL). The proportionality of the magnetic relaxation amplitude to the number of bound labels allows a quantification of the antibody binding capacity. 相似文献
12.
在Tris-HCl缓冲溶液(pH=7.4)中,分别研究了莫西沙星、诺氟沙星、氧氟沙星、恩诺沙星、洛美沙星、环丙沙星与牛血清白蛋白的相互作用,利用计算药物小分子与蛋白结合常数的常用方程:Scatchard修正方程、Lineweaver-Burk双倒数方程、双对数方程、修正的Stern-Volmer方程、改进的双对数方程,分别计算了各沙星药物与蛋白的结合常数和结合位点数。对计算结果进行比较,结果表明对于药物与蛋白为1∶1的结合反应,除Scatchard修正方程外其他方程的计算结果十分接近,其中改进的双对数方程的计算结果更加合理准确。 相似文献
13.
Various metal ions bind to the protein α-lactalbumin prepared from goat milk. The stability of the protein after metal binding is compared with that of the apo-protein by monitoring the fluorescence of the tryptophan residues under equilibrium conditions. The kinetics of the metal binding is studied by stopped-flow fluorescence spectroscopy. By means of the Arrhenius plots, the activation energy with regard to the binding of the different ions is determined. 相似文献
14.
Boontarika Chanvorachote Ubonthip Nimmannit Walaisiri Muangsiri Lee Kirsch 《Journal of fluorescence》2009,19(4):747-753
A fluorescence method for determining the mode of binding and estimating binding parameters in a model drug-carrier complex
was developed using the lipopeptide antibiotic daptomycin and polyamidoamine (PAMAM) dendrimer. Mathematical simulations of
model equations describing fluorescence changes induced by antibiotic–carrier binding in terms of total drug concentration
were used to evaluate the sensitivity of parameter variation on binding isotherms for both one- and two-site binding models.
Nonlinear regression analysis was used to estimate binding parameters and to identify pH-dependent binding models. 相似文献
15.
Belal H M Hussein Mostafa A Gouda Omer Sakin Abdulla Faluji Mohamed Gomaa Hassan A Azab Walid Fathalla Sherin Arabi Sawsan Mosa 《光谱学与光谱分析》2018,38(11):3611-3621
A new europium (Ⅲ) complex containing (4-Methyl-2-oxo-2H-chromen-7-yloxy)-acetic acid moiety (CMMC) was synthesized, characterized, and confirmed as antitumor agent and fluorescent probe. The spectroscopic measurements of Eu(Ⅲ) in the presence of CMMC were obtained in different solvents. The results show that the strongest Eu(Ⅲ) emission bands were monitored in iso-propyl alcohol while the weakest Eu(Ⅲ) emission band was observed in acetonitrile. The interaction of Eu(Ⅲ)-(CMMC)2 complex with DNA was monitored using absorption and emission techniques. From fluorescence titration measurements, the binding constants of DNA with Eu(Ⅲ)-(CMMC)2 complex were found to be 1.04×105 L·mol-1 in Tris-HCl and 1.17×107 L·mol-1 in DMSO-Tris-HCl buffer (9∶1 V/V). Hypochromism was observed from the absorption titration experiment which indicates the intercalation of Eu(Ⅲ)-complex between the base pair of DNA. This result further confirmed by fluorescent Ethidium bromide displacement assay. The fluorescence calibration curve was used for the determination of DNA with LOD of 1.2 ng in DMSO-Tris-HCl buffer (9∶1 V/V) and 5 ng in Tris-HCl buffer. The preliminary antitumor investigation shows promising cytotoxicity against MDA-MB-231, MCF-7 (mammary cancer), and PC-3 (prostate carcinoma) cell lines with IC50 values of 40.63, 25.42 and 30.25 μmol·L-1, respectively. 相似文献
16.
对牛血清白蛋白(BSA)与盐酸特拉唑嗪(THD)混合体系进行了荧光发射光谱和同步光谱实验研究。BSA溶液在340 nm处有明显荧光特征峰,当加入THD后导致牛血清白蛋白发生内源荧光猝灭。据此建立了以BSA为探针的THD含量测定方法,通过该方法所得的模型函数相关系数都高于0.99。对所得模型函数进 行了实验验证,其中发射光谱模型函数回收率为96.04%~103.16%,同步光谱模型函数回收率为100.41%~ 105.58%,相对标准偏差分别为3.70%和2.42%。荧光发射光谱和同步光谱方法的检出限分别为0.64×10-8 mol/L和0.66×10-8 mol/L,定量限分别为0.21×10-7 mol/L和0.22×10-7 mol/L。 相似文献
17.
The constants of binding dye molecules with the micelles of sodium dodecyl sulfate are determined using quenching of delayed fluorescence of acridine dyes by sodium iodide in aqueous–micellar solutions. Kinetic equations have been composed that describe the processes of deactivation of the excited states of dyes. By solving these equations at the concentration of the quencher sodium iodide corresponding to the minimum lifetime of triplet states and at the concentration of micelles corresponding to the least value of the delayed fluorescence quenching rate constants, we obtained the constants of binding dyes with micelles equal to 1.3·107, 2.9·107, and 3.1·107 M–1 for trypaflavine, acridine orange, and acridine yellow, respectively. We calculated the rate constants of quenching of the triplet states of the molecules of dyes by iodide ions (I
–) that decreased in transition from trypaflavine to acridine orange and acridine yellow. 相似文献
18.
The DNA binding and photocleavage specificities of the Zn(II), Cu(II), Co(III), Mn(III) complexes of 5,10,15-tris(1-methylpyridinium-4-yl)-20-(4-propionoxyphenyl)porphyrin have been studied by using a combination of absorption, fluorescence titration, surface-enhanced Raman spectroscopy (SERS), induced circular dichroism (ICD) spectroscopy, thermal DNA denaturation as well as gel electrophoresis experiment. It is found that Cu(II) porphyrin has comparable binding ability with the free base porphyrin while the axial-coordinated Zn(II), Co(III), and Mn(III) porphyrins have lower Kb because of the molecular steric hindrance. However, those metalloporphyrins with lower Kb have similar DNA cleavage efficiencies with the free base porphyrin. This could be best understood by the enhancement of the 1O2 productivity which may also result from the steric hindrance of the axial-coordinated metalloporphyrins. 相似文献
19.
Investigation of the Interaction Between Rutin and Trypsin in Solution by Multi-Spectroscopic Method
ABSTRACT The interactions between rutin and trypsin were investigated by UV-Vis absorption, CD, fluorescence, resonance light-scattering spectra, synchronous fluorescence, and three-dimensional fluorescence spectra techniques under physiological pH 7.40. Rutin effectively quenched the intrinsic fluorescence of trypsin via static quenching. The enthalpy change and entropy change were estimated to be ?8.23 kJ·mol?1 and 53.66 J·mol?1·K?1 according to the van't Hoff equation. The process of binding rutin to trypsin was a spontaneous molecular interaction procedure. This result indicates that hydrophobic and electrostatic interactions played a major role in stabilizing the complex. The conformation of trypsin was discussed by CD, synchronous, and three-dimensional fluorescence techniques. 相似文献
20.
本文采用均匀沉淀法,在室温条件下,合成了较为稳定的ZnO量子点。用荧光光谱法、紫外可见分光光度法分析了ZnO量子点与头孢哌酮的相互作用,用Stern-Volmer方程研究了头孢哌酮对ZnO量子点的荧光猝灭作用,结果表明属于静态荧光猝灭,计算了不同温度时的猝灭常数(292K:9.550×103 L·mol-1、303K:5.980×103 L·mol-1、313K:4.412×103 L·mol-1)和热力学参数,证明二者主要以范德华作用和氢键作用力结合。根据Forster的偶极-偶极非辐射能量转移原理计算出结合位置距离色氨酸残基2.47nm,发生分子内的非辐射能量转移。为探讨纳米颗粒与此类药物分子之间相互作用的化学机理提供了重要的信息。 相似文献