大肠癌与微量元素研究

对48例大肠癌患者手术前后血清和正常大肠组织与癌组织中微量元素测定、比较，发现大脑癌患者手术前后血清微量元素变化，除Cr、Mn有显著差异外均无显著差异，而癌瘤组织与正常大脑组织中Ca、Cd、Mn、Zn、Cu/Zn有明显差异，探讨了大肠癌发生的机理。大肠癌病因是复杂的，但与微量元素改变有一定关系，并指出，组织测定观察微量元素变化比血清测定观察更敏感。     

稀土和微量元素对鸡胚生长发育影响的研究

研究了稀土和微量元素对鸡胚生长发育的影响及吸收分布情况。以海兰种蛋为实验材料,在稀土中按一定比例添加不同量的7种生物必需微量元素,注入种蛋进行孵化,实验发现,稀土与微量元素共用对鸡胚生长发育有一定影响;稀土剂量一定,加入不同量的微量元素,稀土和微量元素会产生最佳配比;最佳配比能使鸡胚正常生长发育,且稀土在各组织中的积累较使用同剂量的单一稀土少。     

饮茶与微量元素

茶叶是备受我国人民及世界人民欢迎的饮料,饮茶不仅仅是能得到味觉的享受,还能得到保健身体的效果,茶还提供人体组织正常运转所不可缺少的矿物质元素,茶叶的保健效果主要取决于它的抗氧化和含有多种丰富的人体所必需的宏量元素和微量元素。经过国内外科学家的研究发现,茶叶中所含的必需宏量元素和微量元素约有40种之多,含有较丰富的必需微量元素有铁、锌、锰、铬、氟和硒等等。     

食管癌组织中铜含量及其临床意义

应用日立１８０－３０型原子吸收火焰分光光度计，对３６例食管癌组织中的微量元素Ｃｕ进行了测定，并与３０例食管癌患者的癌旁正常食管组织进行了对照比较。结果表明，癌组织Ｃｕ含量显著高于癌旁组织Ｃｕ含量。     

编者的话--切盼我国的人体头发和血液微量元素正常参考值诊断标准早日出台

本期发表了我国资深的微量元素与健康研究科学家、中国微量元素科学研究会名誉理事长秦俊法教授等撰写的“人体头发和血液微量元素正常参考值的确定原则及方法”论文，这篇文章在目前形势下发表有非常积极的意义。我国搞微量元素与健康研究、微量元素与临床应用研究30多年，发表了有关人体头发和血液微量元素正常参考值论文几千篇，出版了人体微量元素数据手册近十种版本，建立了两个国家级的微量元素数据库，积累了数以百万计人体及其他生物的微量元素数据。各省市也陆续出台了地方性的人发和血液微量元素正常参考值，这些参考值已应用了30多年，接受了临床实践检验30多年。     

肝癌及癌旁组织中微量元素含量研究

为探讨肝癌患者的癌、癌旁和正常组织中微量元素含量的变化 ,收集了 3 0例肝癌患者的癌、癌旁及正常组织 ,应用等离子体发射光谱仪测定了Se、Zn、Cu、Fe、Mg、Mn、Mo的含量。结果表明 ,肝癌患者的正常、癌旁、癌组织Se、Zn、Mg、Mo的含量和Se/Cu、Zn/Cu、Fe/Cu、Mg/Cu、Mo/Cu的比值逐渐降低 (P <0 0 1～ 0 0 5 ) ,但Cu、Mn的含量则相反逐渐升高 ,Fe的含量和Mn/Cu的比值无显著差异 (P >0 0 5 )。本研究提示 ,微量元素含量的变化与肝癌发生、发展有相关性 ,对癌变过程的研究和临床治疗的指导具有重要意义     

西安市某辖区2～6岁儿童全血样品微量元素含量状况调查

摘要对西安市某辖区妇幼保健站及多家幼儿园920名2～6岁身体健康儿童的全血样品进行微量元素检测,统计得到该区域正常儿童微量元素的含量范围,为准确了解西安地区某区域2-6岁正常儿童体内微量元素正常含量提供了参考数据。     

锌在食管癌变中的作用

应用日立１８０－３０型原子吸收火焰分光光度计，对３６例食管癌组织中的微量元素锌进行了测定，并与３０例食管癌患者的癌旁正常食管组织和１２例正常人的食管组织进行了对照比较，结果表明：癌组织锌含量明显低于癌旁组织和正常食管组织锌含量，癌旁组织锌含量又显著低于正常食管组织锌含量，有淋巴结转移和恶性程度高的食管癌组织中锌含量明显减少，提示锌缺乏时可以诱发食管癌，在食物中补充锌等能预防食管癌的发生。     

电感耦合等离子体质谱法测定血液中14种微量元素

<正>微量元素在人体中的含量及代谢与人体健康有着密切的关系。当人们体内进行各种生理活动或者参加外界运动时,微量元素将起到不同的功效,摄入的量过多、不足或不平衡都会不同程度地引起人体生理的异常或疾病的发生~([1-2])。在患者未出现临床症状之前,在血液、尿液、活体组织(如毛发、指甲等)中可发现一些微量元素及代谢衍生物的变化。目前一些医疗机构通过检测头发或者血液中的微量元素,来判断受检者体内微量元素含量是否正常。     

必需微量元素与非必需微量元素

根据机体对微量元素的需要情况,可将其分为必需微量元素和非必需微量元素。维持生物体正常生命活动不可缺少的微量元素称必需微量元素。所谓不可缺少,并非指缺少时将危及生命不能生存,而是指缺少时会引起机体生理功能及结构异常,导致疾病发生。SCHROEDER、COTZIASOUS和DAVIES分别提出过判断必需元素的标准其要点包括以下几项:①广泛存在于自然界,海水中含量丰富,能被动植物吸收利用;②是形成正常组织结构的组成部分,存在于一切健康机体的所有组织之中且含量相当稳定;③一旦缺乏这种元素,无论是何种生物,都会产生相似的结构及生理功能异常和生物化学改变,充分供应该元素可预防此类异常,给缺乏者补充该元素则可使异常得到纠正;④能透过半透膜,即能通过胎盘和乳房屏障供给胎儿和婴儿;⑤以其天然形态适当剂量给人或动物服用,无毒害作用或毒性极低。此外,机体对该类元素有平衡调节能力;这类元素在元素周期表中处于一定位置,新发现的必需元素的原子序数大多排列在那些已知必需元素之间。目前多数人公认的必需微量元素有:铁(Fe)、铜(Cu)、锌(Zn)、钴(Co)、锰(Mn)、铬(Cr)、钼(Mo)、镍(Ni)、钒(V)、锡(Sn)、硅(Si)、...     

负载顺铂的泊洛沙姆温敏凝胶用于小鼠宫颈癌的局部化疗

为了探讨载有顺铂的温敏凝胶对小鼠宫颈癌的局部化疗作用,制备了载有顺铂的泊洛沙姆温敏凝胶,并通过流变学、体外溶蚀、体外释放等技术手段表征其结构和性能,采用鼠源宫颈癌U14细胞制备了原位宫颈/阴道癌模型,将载药凝胶灌注入荷瘤鼠阴道,评价载药凝胶对小鼠宫颈/阴道癌的抑制作用。结果表明,载有顺铂的泊洛沙姆温敏凝胶经阴道给药后对小鼠宫颈/阴道癌的抑瘤率可达到63.1%,可有效抑制小鼠宫颈癌的生长,而系统安全性则远远高于静脉注射顺铂。因此,载有顺铂的温敏凝胶有可能成为一种新型的宫颈癌治疗剂型。     

Studies on changes in trace elemental content of serum of uterine cervix cancer patients using PIXE

The objective of this study was to evaluate the levels of trace elements in blood sera of uterine cervix cancer patients, analyze their alteration with respect to healthy controls and identify the best predictors amongst these for disease occurrence and progression. Particle induced X-ray emission (PIXE) technique was used in this work to identify and quantify trace elements Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Se, Br in the blood sera of uterine cervix cancer subjects and healthy control subjects. The observed alterations are discussed with respect to the possible mechanisms by which these elements might influence the carcinogenic process.     

海洋珍珠生物提取液单独与顺铂联合对人宫颈癌Siha细胞的生长抑制作用

为探讨海洋珍珠生物提取液对人宫颈癌Siha细胞体外生长的抑制作用,以及海洋珍珠生物提取液增加Siha细胞对顺铂的敏感性,以期对治疗宫颈癌及其它肿瘤寻找新的治疗药物,体外培养人宫颈癌Siha细胞,分别用海洋珍珠生物提取液、顺铂、海洋珍珠生物提取液加顺铂处理24、48、72 h,采用四甲基偶氮唑蓝(MTT)比色法检测海洋珍珠生物提取液和顺铂及两者联合应用Siha细胞的存活率。结果表明,海洋珍珠生物提取液对Siha细胞的增殖有明显的抑制作用(P0.01),其处理,24、48、72 h的IC50分别为56.26、51.92、48.92μg/mL,顺铂对Siha细胞的24、48、72 h的IC50分别为16.74、4.68、6.69μg/mL;两者合用可大大提高对Siha细胞的抑制作用,比单用海洋珍珠生物提取液或单用顺铂均有明显的提高(P0.01)。提示海洋珍珠生物提取液能抑制Siha细胞的生长,同时能增加Siha细胞对顺铂的敏感性。     

Indole-based chemosensor for Hg2+ and Cu2+ ions: applications in molecular switches and live cell imaging

An indole based "ratiometric" and "turn-off" tris(N-methylindolyl)methane based chemosensor depicting a contrasting fluorescent behavior towards Hg(2+) and Cu(2+) ions, exhibited NOR and YES logic functions, and also imaged intracellular Hg(2+) in cervix cancer (HeLa) cells.     

Copper (II)-Surfactant Complex and Its Nano Analog as Potential Antitumor Agents

The in vitro anticancer activity of copper cetyl trimethyl ammonium bromide (Cu-CTAB)-loaded cyclodextrin nanoparticles on Ehrlich ascites carcinoma (EAC), colon cancer cells (HCT 116), liver cancer cells (HepG-2), breast cancer cells (MCF-7), and cervix cancer cells (Hela) was investigated using MTT due assay. Cyclodextrin nanoparticles loaded with Cu-CTAB exerted in vitro anticancer activity against the previous human cancer cell lines comparable to the activity of free non-entrapped in nanoparticles macro-particle Cu-CTAB. The nano analog was synthesized by physical loading using grinding with ball mill. The ratio between Cu-CTAB and cyclodextrin oligosaccharide was 1 Cu-CTAB: 3 cyclodextrin. The particle size of the nano derivative was determined using the transmitted electron microscope (TEM).     

Synthesis,anti‐inflammatory,and anticancer activity evaluation of some heterocyclic amidine and bis‐amidine derivatives

Heterocyclic amidine derivatives of benzothiazole ( 6a , 6b , 6c ), benzimidazole ( 6d , 6e , 6f ), benzoxazole ( 6g , 6h , 6i ), and bis‐amidine derivatives of pyrimidine, ( 7a , 7b ) & triazole ( 7c , 7d , 7e ) ring system have been synthesized by nucleophilic addition reaction. All these compounds were screened for anti‐inflammatory and anticancer activities. At a dose of 50 mg/kg p.o., compounds 6c (39%), 6e (39%), and 6f (39%) exhibited anti‐inflammatory activity comparable to standard drug ibuprofen, which showed 39% activity and compounds 6b , 6e , 7a , and 7c exhibited moderate anticancer activity against cervix (HELA); neuroblastoma (IMR‐32); breast (MCF‐7), leukemia (THP‐1); and cervix (HELA) human cancer cell lines, respectively. J. Heterocyclic Chem., (2011).     

Synthesis and Antitumor Activity of 4-tert-Butyl-5-benzyl-2-benzyliminothiazoles

A series of novel Schiff bases including 4-tert-butyl-5-benzyl-2-benzyliminothiazoles was synthesized by reacting the aromatic aldehydes with the corresponding 2-aminothiazoles.The antitumor bioassay revealed that compounds 2n and 2m exhibited potent cytotoxicity against human cervix cancer（HeLa） cell line with IC50 values of 0.001 and 0.007 mmol/L,respectively.The preliminary structure-activity relationship（SAR） investigations and the apoptosis evaluation suggest that 4-tert-butyl-5-benzyl-2-benzyliminothiazoles may be a satisfactory backbone for antitumor activity,and compound 2n can serve as an attractive candidate for the development of novel apoptosis in anticancer treatment.     

Arenicolsterol A，来自海洋环节动物海蚯蚓中的一种新的细胞毒性烯醇式磺化甾醇

Marine organisms are the important source of the bioactive metabolites. A novel enolic sulfated sterol, arenicolsterol A, has been isolated from a marine annelid Arenicola cristata collected in the coast of Mainland of China.The structure was elucidated using all sorts of spectroscopic data including ESIMS, 1D and 2D NMR etc. The cytotoxic bioactivity of this sterol was evaluated by MTT assay. It could inhibit the growth of human cervix cancer cell line (Hela) and human non-small cell lung cancer cell line (NCI-h6) with IC50 of (3.1 0.6)μg/mL and (7.6 0.8)μg/mL.     

Beijing synchrotron radiation total-reflection X-ray fluorescence analysis facility and its applications on trace element study of cells

The feasibility of total-reflection X-ray fluorescence (TXRF) analysis excited by synchrotron radiation applied to trace element analysis of biological cells is investigated. The Beijing synchrotron radiation TXRF facility and the related experimental method are also described. The elemental minimum detection limits of some standard reference materials are determined. The elemental compositions of a cluster of small intestine cells of a small white mouse are given, and hence the average trace element contents of the single small intestine cell are also obtained. With this technique, the changes of some trace elements in the cells of lung and cervix cancer before and after apoptosis are also preliminarily studied.     

E-cadherin expression is biochemical marker in cervical intraepithelial neoplasia

The expression of E-cadherin has been investigated by the immunohistochemical method. The expression of E-cadherin is considered as a biomarker of junctions of intercellular adhesion in tissues samples with cervical intraepithelial neoplasias. The quantitative avidin-biotin-peroxidase method was used to estimate the expression. Precancer lesions, i.e., cervical intraepithelial neoplasias, CIN 1, 2, 3, precede invasive cancer of the cervix uteri by several years, the prognosis of which is variable in terms of regression and progression into invasive cancer. The expression of E-cadherin has been analyzed in cervical tissues of patients with various grades of CIN and microinvasive cancer (81 samples), as well as patients without CIN (ten samples). The sharp, statistically significant decrease in the expression of E-cadherin in CIN3 has been recorded compared to CIN1, CIN2, and the normal epithelium. It is reasonable to use this marker to assess the grade of the lesion and develop an individual prognosis of CIN progression. The data present a new way to construct pharmaceuticals based on compounds that influence E-cadherin expression.