共查询到20条相似文献,搜索用时 46 毫秒
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相衬显微镜的简易实验方法白晓林(山西医学院物理教研室030001)刘珍(山西职工医学院030012)相衬显微镜相板设计、安装难以实现,且位相概念抽象,学生较难理解.本文提出一种简易方法演示相衬显微镜以解决教学难点.一、理论分析设光通过物体后复振幅分布... 相似文献
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泽尔尼克与相衬显微镜 总被引:3,自引:1,他引:2
相衬法是通过空间滤波器将物体的位相信息转换为相应的振幅信息,用改变频谱的位相来改善透明物体成像的反衬度,从而提高透明物体的可分辨性,泽尔尼克首先提出相衬法并发明了相衬显微镜.相衬显微镜现已在生物学、医学、晶体学、高分子材料结构性能的研究和矿物晶体微形貌学进行晶体表面生长的动态观察中获得了相当好的效果. 相似文献
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介绍了泽尼克的生平和他对物理学发展的主要贡献,着重介绍了他是如何发明相衬原理和相衬显微镜的,以及这一发明对我们的启示-创新将面临重重困难,为了增强学生的创新能力,必须注意理论与实验结合能力的培养。 相似文献
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M. Kageyama S. Tasaki M. Hino T. Ebisawa D. Yamazaki H. Hayashida Y. Abe 《Physica B: Condensed Matter》2009,404(17):2615-2619
We present an imaging technique utilizing a neutron spin interferometer. Neutron spin phase contrast is achieved in spatial resolved measurements of the phase difference between two superposed neutron spin states introduced by passing through a magnetic sample. Since the phase difference of spin states parallel and anti-parallel to the magnetic field is proportional to the magnetic field integral, it is possible to record images of the internal magnetic field distribution of the sample. Taking advantage of high transmission probabilities, neutron spin phase contrast provides non-destructive images of internal magnetic structures. 相似文献
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We develop an element-specific x-ray microscopy method by using Zernike phase contrast imaging near absorption edges, where a real part of refractive index changes abruptly. In this method two phase contrast images are subtracted to obtain the target element: one is at the absorption edge of the target element and the other is near the absorption edge. The x-ray exposure required by this method is expected to be significantly lower than that of conventional absorption-based x-ray elemental imaging methods. Numerical calculations confirm the advantages of this highly efficient imaging method. 相似文献
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A new method for information retrieval in two-dimensional grating-based X-ray phase contrast imaging 下载免费PDF全文
Grating-based X-ray phase contrast imaging has been demonstrated to be an extremely powerful phase-sensitive imaging technique.By using two-dimensional(2D) gratings,the observable contrast is extended to two refraction directions.Recently,we have developed a novel reverse-projection(RP) method,which is capable of retrieving the object information efficiently with one-dimensional(1D) grating-based phase contrast imaging.In this contribution,we present its extension to the 2D grating-based X-ray phase contrast imaging,named the two-dimensional reverseprojection(2D-RP) method,for information retrieval.The method takes into account the nonlinear contributions of two refraction directions and allows the retrieval of the absorption,the horizontal and the vertical refraction images.The obtained information can be used for the reconstruction of the three-dimensional phase gradient field,and for an improved phase map retrieval and reconstruction.Numerical experiments are carried out,and the results confirm the validity of the 2D-RP method. 相似文献
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Single-shot grating-based x-ray differential phase contrast imaging with a modified analyzer grating 下载免费PDF全文
X-ray grating interferometer has attracted widely attention in the past years due to its capability in achieving x-ray phase contrast imaging with low brilliance source. However, the widely used phase stepping information extraction method reduces system stability and prolongs data acquisition time by several times compared with conventional x-ray absorptionbased imaging. The mechanical stepping can be avoided by using a staggered grating, but at the cost of low vertical spatial resolution. In this paper, employing a modified staggered grating and the angular signal radiography, we proposed a single-shot grating-based x-ray differential phase contrast imaging with decent vertical spatial resolution. The theoretical framework was deduced and proved by numerical experiments. Absorption, phase, and scattering computed tomography can be performed without phase stepping. Therefore, we believe this fast and highly stable imaging method with decent resolution would be widely applied in x-ray grating-based phase contrast imaging. 相似文献
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Microbubbles containing gadolinium as contrast agents for both phase contrast and magnetic resonance imaging 下载免费PDF全文
Rongbiao Tang Fuhua Yan Guo-Yuan Yang Ke-Min Chen 《Journal of synchrotron radiation》2018,25(2):560-564
Portal vein imaging is an important method for investigating portal venous disorders. However, the diagnostic requirements are not usually satisfied when using single imaging techniques. Diagnostic accuracy can be improved by combining different imaging techniques. Contrast agents that can be used for combined imaging modalities are needed. In this study, the feasibility of using microbubbles containing gadolinium (MCG) as contrast agents for both phase contrast imaging (PCI) and magnetic resonance imaging (MRI) are investigated. MCG were made by encapsulating sulfur hexafluoride (SF6) gas with gadolinium and lyophilized powder. Absorption contrast imaging (ACI) and PCI of MCG were performed and compared in vitro. MCG were injected into the main portal trunk of living rats. PCI and MRI were performed at 2 min and 10 min after MCG injection, respectively. PCI exploited the differences in the refractive index and visibly showed the MCG, which were not detectable by ACI. PCI could facilitate clear revelation of the MCG‐infused portal veins. The diameter of the portal veins could be determined by the largest MCG in the same portal vein. The minimum diameter of clearly detected portal veins was about 300 µm by MRI. These results indicate that MCG could enhance both PCI and MRI for imaging portal veins. The detection sensitivity of PCI and MRI could compensate for each other when using MCG contrast agents for animals. 相似文献