Precision analysis in polarization-resolved second harmonic generation microscopy

Polarization-resolved second harmonic generation (PSHG) imaging microscopy allows one to provide information such as anisotropy parameters and molecule orientation. We analyze the precision of parameter estimation for samples with cylindrical symmetry and a Gaussian additive noise model. We introduce improvements of existing techniques that can be useful to get rapid acquisition and processing of PSHG images, and we discuss the influence of photon noise.     

Imaging the structure and organization of mouse cerebellum and brain stem with second harmonic generation microscopy

To visualize the structure and organization of the brain is a fundamental requirement in the research of neuroscience. Here, combining with two-photon excitation fluorescence microscopy and transgenetic mouse GAD67,we demonstrate a custom-built second harmonic generation(SHG) microscope to discriminate brain layers and sub regions in the cerebellum and brain stem slices with cellular resolution. In particular, the cell densities of neurons in different brain layers are extracted due to the cell soma appearing as dark shadow on an SHG image.Further, the axon initial segments of the Purkinje cell are easily recognized without labeling, which would be useful for guiding micropipettes for electrophysiology.     

应用级联倍频方法提高倍频系统输出稳定性研究

提出应用级联倍频方法提高倍频系统输出稳定性,并就该方法的有效性进行了理论分析和模拟计算.分析和计算结果不但证明级联倍频方法能实现倍频系统稳定输出,而且还表明可以通过仔细调节第一块倍频晶体中波矢方向 k 与光轴间夹角、两块倍频晶体间的间隔,能调节改变实现倍频系统最稳输出时所需第二块晶体的理论计算长度,使之与第二块倍频晶体的实际加工长度一致,最终实现系统稳定倍频输出.级联倍频方法在实现高输出稳定性的同时能实现高的倍频转换效率,对应用于光参量啁啾脉冲放大系统的高稳定抽运源系统的设计建造具有重要参考意义. 关键词： 级联倍频 稳定倍频输出 光参量啁啾脉冲放大     

Coherence artifacts in second harmonic microscopy

We present an analysis of these effects and demonstrate a simple, yet powerful technique to eliminate these coherence artifacts. The technique relies on a superposition of the signal field with a coherent auxiliary SH-field of controllable phase and amplitude and allows a direct observation of the true χ⁽²⁾-morphology of the interface. Recieved: 20 September 1998     

相位失配法实现倍频稳定输出的模拟分析

 对BBO晶体Ⅰ类相位匹配倍频过程和KTP晶体XY平面内的Ⅱ类相位匹配倍频过程进行数值模拟,结果表明：Ⅰ类和Ⅱ类相位匹配倍频过程,都可通过引入适量的相位失配量实现稳定倍频输出;随着相位失配量的增大,实现稳定倍频输出所需的晶体长度都变小,倍频稳定输出起伏变小,倍频转换效率有不同程度的下降;相对于XY平面内的Ⅱ类相位匹配倍频过程,BBO晶体的Ⅰ类相位匹配倍频过程对相位匹配偏离角更敏感,且稳定输出时倍频转换效率更低。     

Self pulsing in second harmonic generation

We consider nonlinear optical processes in a cavity maintained far from thermal equilibrium by external pumping. In second harmonic generation we predict a hard mode instability at a critical pump strength indicating the existence of undamped spiking similar to that in strongly pumped lasers. In subharmonic generation above the soft mode transition noted by Graham we predict the appearance of sidebands in the subharmonic spectrum.     

Noncollinear second harmonic generation in KDP

This paper reports experimental and analytical results on optical second harmonic generation when a noncollinear arrangement is employed. The dependence of second harmonic power on crystal length, absorption, beam radius and the angle between the two fundamental waves is obtained. Double refraction of the crystal is taken into account, but the divergence and depletion of the fundamental waves are neglected. The results of the calculations are confirmed by experiments carried out with a KDP crystal in the near field of two intersecting TEM₀₀ ruby laser beams.An extension of the theory to nonlinear interactions other than second harmonic generation - e.g. four-wave interaction - is possible.     

Efficient second harmonic generation in CDA

Efficient second harmonic generation at 0.532 μ has been achieved in a temperature tuned CDA crystal when a narrow beam divergence Nd:YAG laser was used. At a crystal temperature of 40.3°C, a peak second harmonic power of 7.5 MW was obtained with a peak power conversion efficiency of 33%. The temperature variation of the refractive indices in CDA has been determined to be d(n^e_2ω?n⁰_ω)/dT = 7.2 × 10^?5°C^?1.     

Surface second harmonic generation of chiral molecules using three-coupled-oscillator model

Based on the three-coupled-oscillator molecular model we proposed, the relation between the second-order susceptibilities of a chiral film and the molecular hyperpolarizabilities is given. The effect of microscopic parameters on the second-order susceptibilities is simulated numerically and the difference between the efficiencies of s-polarized second-harmonic fields induced by the left- and the right-handed circularly-polarized fundamental beams is discussed. The theoretical basis for studying second-order nonlinear optical properties of the chiral molecular media with a tripod-like structure is provided in this paper.     

相位失配法实现倍频稳定输出的模拟分析

对BBO晶体Ⅰ类相位匹配倍频过程和KTP晶体XY平面内的Ⅱ类相位匹配倍频过程进行数值模拟,结果表明：Ⅰ类和Ⅱ类相位匹配倍频过程,都可通过引入适量的相位失配量实现稳定倍频输出;随着相位失配量的增大,实现稳定倍频输出所需的晶体长度都变小,倍频稳定输出起伏变小,倍频转换效率有不同程度的下降;相对于XY平面内的Ⅱ类相位匹配倍频过程,BBO晶体的Ⅰ类相位匹配倍频过程对相位匹配偏离角更敏感,且稳定输出时倍频转换效率更低。     

二次谐波产生过程中准相位匹配条件的研究

从耦合波基本方程出发,在实空间严格推导出二次谐波产生(SHG)过程中的准相位匹配(QPM)条件.讨论了满足QPM条件时,SHG的转换效率以及相位失配角随波传播距离的变化.着重研究了非线性极化率的周期性调制对提高SHG转换效率的贡献.     

Optical second harmonic generation in ammonium tartrate

Second harmonic generation was observed in ammonium tartrate single crystals by a phase matching technique at the fundamental wavelength 1.153 μm. The nonlinear coefficients were found to be comparable in magnitude to those of KDP. The optical transmission and the refractive indices were also measured.     

Mode-coupling phenomena in intracavity second harmonic generation

The dependence of intracavity second harmonic generation efficiency on the linear phase detunings in crystal and air is studied both theoretically and experimentally. It is shown that in the presence of feedback at the second harmonic frequency an optimum conversion efficiency can be achieved by a mutually correlated choice of phase mismatch in a nonlinear crystal and the position of the latter inside the cavity.     

Optical second harmonic generation in ammonium malate

Second harmonic generation in ammonium malate has been investigated in a wavelength region between 492 nm (short wavelength limit of the harmonic beam at 246 nm) and 640 nm. Phase match angle, refractive indices and all coefficients of the nonlinear susceptibility tensor have been measured; several possibilities for phase matching are discussed.     

On the theory of second harmonic generation

It is shown how the theory of non-linear interaction between the modes of the open resonator, developed previously [1] and used to study resonant second harmonic generation [2], can be applied to second harmonic generation in the absence of a resonator. The conditions under which the second harmonic output, in the small conversion approximation, can be approximated by a Gaussian beam are derived.The optimum value of the focusing parameter I/z₀ (hereI is the length of the crystal, andz ₀ is one-half the confocal parameter of the fundamental beam) for the total output is shown to be 5.68 agreeing precisely with the value given by Boyd and Kleinman [3].     

Monitoring photo-thermal response and collagen remodeling of mouse skin with multiphoton microscopy

Two-photon excitation fluorescence （TPEF） and second-harmonic generation （SHG） are detected through multiphoton microscopy （MPM）. The major signals have the potential to monitor the process of tissue changes. TPEF and SHG are used to monitor the skin photo-thermal response to irradiation with intense pulsed light sources （A is in the range of 560-1200 nm） and trace the process of skin remodeling in vivo at different time intervals. TPEF intensity is nearly unchanged at different time intervals after irradiation, whereas SHG intensity changes considerably. The results reveal the photo-thermal effect of nonablative light sources and the process of collagen remodeling at the sub-micron level.     

Topography of antiferromagnetic domains using second harmonic generation with an external reference

2 O₃, in which the time-invariant second-harmonic reference can be generated either intrinsically by the crystal or externally by use of the new technique, so that both methods may be compared. The technique is then used to visualize the domain structure of YMnO₃, an antiferromagnetic crystal which does not provide the time-invariant reference wave intrinsically. The experiments demonstrate the wide applicability of the method. Received: 13 June 1997