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1.
Multivariate curve resolution with alternating least squares (MCR-ALS) is applied for the first time to the simultaneous analysis of electrochemical and spectroscopic data. Then, a data analysis is done with augmented matrices constituted by Differential Pulse Polarography and Circular Dichroism data submatrices. The use of proper, and different for each submatrix, constrains in the iterative ALS optimization allows to obtain chemically meaningful results constituted by a common matrix containing the concentration profiles, and two matrices with the pure electrochemical and spectroscopic signals. MCR-ALS is applied to the study of the complexation of Cd by Cys-Gly, a glutathione-fragment of great interest for understanding metal-phytochelatins complexation. 相似文献
2.
A new mathematical algorithm is proposed to correct the progressive potential shift of some voltammetric signals that decrease the linearity of the data. The corrected data matrix can be further analysed by Multivariate Curve Resolution by Alternating Least Squares (MCR-ALS) and the vector including the potential shift corrections can be fitted to specific equations such as that by DeFord-Hume. A detailed discussion is given on the different cases of potential shift correction, and, in some of them, mathematical simulation is made or experimental systems [Cd(ii)-glutathione and Zn(ii)-glycine] are analysed. 相似文献
3.
Tatenda Jackfama Tichaona Nharingo Munyaradzi Shumba Jonathan Okonkwo 《International journal of environmental analytical chemistry》2019,99(1):47-60
In this study, we demonstrated a highly sensitive electrochemical sensor for the simultaneous detection of Pb (II) and Cd (II) in aqueous solution using carbon paste electrode modified with Eichhornia crassipes powder by square wave anodic stripping voltammetry. The effect of modifier composition, pH, preconcentration time, reduction potential and time, and type of supporting electrolyte on the determination of metal ions were investigated. Pre-concentration on the modified surface was performed at open circuit. The modified electrode exhibited well-defined and separate stripping peaks for Pb (II) and Cd (II). Under optimum experimental conditions, a linear range for both metal ions was from 10 to 5000 μg L?1 with the detection limits of 4.9 μg L?1, 2.1 μg L?1 for Cd(II) and Pb (II), respectively. The modified electrode was found to be sensitive and selective when applied to determine trace amounts of Cd (II) and Pb (II) in natural water samples. 相似文献
4.
Rui Gusm?o Cristina Ari?o José Manuel Díaz-Cruz Miquel Esteban 《Analytical and bioanalytical chemistry》2009,394(4):1137-1145
Multivariate curve resolution with alternating least squares (MCR-ALS) has been applied to voltammetric data obtained from
analysis of the competitive binding of cysteine (Cys) and cysteine–glycine (Cys-Gly) by Cd(II) as a first approach towards
mixtures of phytochelatins and related compounds in natural media. From different starting points, the possibilities of formation
of mixed complexes and/or displacements between ligands are investigated. Analysis of the resulting unitary voltammograms
and concentration profiles of the resolved components by MCR-ALS suggests that the strongest ligand (Cys-Gly) is able to displace
the weakest (Cys) from its metal complexes, whereas this does not happen in the opposite direction. On the other hand, no
evidence of Cd mixed-ligand complexes was found.
Figure Differential pulse polarograms measured in the independent titrations of 1 × 10-5 mol L-1 Cys, 1 × 10-5 mol L-1 Cys-Gly, and a mixture of Cys-Gly (0.5 × 10-5 mol L-1) and Cys (1 × 10-5 mol L-1) with Cd2+, at TRIS-HNO3 buffer (0.1 mol L-1 and PH 7.5) in the presence of 0.1 mol L-1 KNO3 相似文献
5.
Róbert Rajkó Samira Beyramysoltan Hamid Abdollahi János Eőri Gábor Pongor 《Analytica chimica acta》2015
In this paper the authors have investigated spectroscopic data analysis according to a recent development, i.e. the Direct Inversion in the Spectral Subspace (DISS) procedure. 相似文献
6.
The spiroorthoester synthesis includes several competitives reactions. A way of determining the reactions that are taking place and their sequential order, is presented. The reaction between the phenylglycidylether and gamma-butyrolactone to obtain a spiroorthoester has been monitored by near-infrared spectroscopy (NIR). In addition to the formation of the corresponding spiroorthoester, some parallel processes can occur. By means of two-dimensional correlation analysis, only one reaction is postulated, the one corresponding to the spiroorthoester formation. This was confirmed by recording the NMR spectra of the final product. Applying multivariate curve resolution-alternating least squares (MCR-ALS) to the NIR spectra obtained during the reaction, it has been possible to obtain the concentration values of the species involved in the reaction. The recovered spectra were compared with the experimentally recorded spectra for the reagents (phenylglycidylether, gamma-butyrolactone) and the final product (spiroorthoester) and the correlation coefficients were, in all cases, higher than 0.990. The maximum and minimum limits associated with the ALS solutions were calculated, making it possible to limit to a considerable extent the ambiguity that is characteristic of these curve resolution methods. 相似文献
7.
Different strategies of multivariate data analysis are used to interpret a data base from geological samples. Cluster and correspondence analysis are applied to classify properly 34 chemical elements from 10 representative rock samples (volcanic series from Borovitsa, Rhodopa mountains, Bulgaria). Principal components analysis is also used as display method to visualize the relation between the variables and objects of interest. The multivariate data analysis applied makes it possible to interpret the origin and orogenesis of the samples. 相似文献
8.
Methotrexate (MTX), a common pharmaceutical drug in cancer therapy and treatment of rheumatic diseases, is known to cause severe adverse side effects at high dose. As the side effect may be life threatening, there is an urgent need for a continuous, bed-side monitoring of the nominal MTX serum level in a patient while the chemical is being administered. This article describes a detection of MTX using a flow system that consists two modified gold electrodes. Interaction of MTX with the antibodies fixed on the electrode surface is detected by electrochemical impedance spectroscopy and evaluated using singular value decomposition (SVD). The key finding of this work is that the change in the electrode capacitance is found to be quantitative with respect to the concentration of MTX. Moreover a calibration curve constructed using the principal component regression method has a linear range of six orders of magnitude and a detection limit of 1.65 × 10−10 M. 相似文献
9.
10.
The idea of quality by design (QbD) has been proposed in pharmaceutical field. QbD is a systematic approach to control the product performance based on the scientific understanding of the product quality and its manufacturing process. In the present study, near-infrared (NIR) imaging is utilized as a tool to achieve this concept. A practical use of a chemometrics technique called self-modeling curve resolution (SMCR) is demonstrated with NIR imaging analysis of pharmaceutical tablets containing two ingredients, a soluble active ingredient, pentoxifylline (PTX), and an insoluble excipient, palmitic acid. Concentration profiles obtained by SMCR reveal that the homogenous distribution of chemical ingredients strongly depends on the grinding time and that its process plays a central role in quantitative control, say sustained-release of PTX. In addition, pure component spectra by SMCR indicate a sequential change of specific NIR peak intensities following the increase of the grinding time. The spectra change shows a molecular structure change related to its crystallinity during grinding process. Accordingly, this study clearly demonstrates that NIR imaging combined with SMCR can be a powerful tool to reveal chemical or physical mechanism induced by the manufacturing process of pharmaceutical products and that it may be a solid solution for QbD of pharmaceutical products. 相似文献
11.
The interaction of fulvic acids with Cd(II) ions has been studied by differential pulse anodic stripping voltammetry (ASV), at pH 7.0 and 0.1 M KNO3. Voltammetric data obtained during the titration of mixtures of Cd(II) and fulvic acids at different concentrations have been analyzed using multivariate curve resolution (MCR). The application of this method allowed the resolution of the major contributions involved in the titration experiments. Apart from free Cd(II), two more contributions related with the complexation process were detected and resolved, and their corresponding pure voltammograms and concentration profiles were estimated. Simultaneous analysis of independent voltammetric titrations using the proposed MCR methods is shown extremely recommended because it overcame some of the limitations observed in the analysis of individual titrations. 相似文献
12.
The iterated constrained endmembers (ICE) algorithm is a new method of unmixing hyperspectral images that combines aspects of multivariate curve resolution (MCR) methods in chemometrics and unmixing algorithms in remote sensing. Like many MCR methods, ICE also estimates pure components, or endmembers, via alternating least squares; however, it is explicitly based on a convex geometry model and estimation is carried out in a subspace of reduced dimensionality defined by the minimum noise fraction (MNF) transform. In this paper, we describe the ICE algorithm and its properties. We also illustrate its use on a hyperspectral image of cervical tissue. The unmixing of hyperspectral images presents some unique challenges, and we also outline where further development is required. © 2008 John Wiley & Sons, Ltd. 相似文献
13.
Application of multivariate curve resolution alternating least squares (MCR-ALS), for the resolution and quantification of different analytes in different type of pharmaceutical and agricultural samples is shown. In particular, MCR-ALS is applied first to the UV spectrophotometric quantitative analysis of mixtures of commercial steroid drugs, and second to the near-infrared (NIR) spectrophotometric quantitative analysis of humidity and protein contents in forage cereal samples. Quantitative results obtained by MCR-ALS are compared to those obtained using the well established partial least squares regression (PLSR) multivariate calibration method. 相似文献
14.
Summary The interaction of copper(II) salts with the imidazole-containing disulphide 5-(1,2,5-dithiazaepan-5-ylmethylene)-4-methyl-2-ethyl imidazole (MAMI) in MeOH have been investigated. The 11 Cu(ClO4)2MAMI system exhibited a single ligand field band at ca. 12200cm-1, an intense shoulder at ca. 31500 cm-1 and a less intense split feature at 24400 and 25300cm-1 assignable to S() CuII and S() CuII charge transfer (CT) transitions, respectively. The e.p.r. parameters suggested the presence of a CuN2SO chromophore, however; the 11 Cu(NO3)2MAMI system did not exhibit a S CuII CT band and the g
value was comparatively high. An electrochemical study of the 11 Cu(ClO4)2MAMI system in MeOH revealed that the copper-disulphide interaction, though weaker, would confer a high redox potential as well as reversibility, similar to the copper-thioether interaction. 相似文献
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16.
Mixtures of acetylsalicylic acid (ASA), paracetamol (PAR), and caffeine (CAF) have been successfully analyzed by constrained multivariate curve resolution-alternating least squares (MCR-ALS). The MCR-ALS methodology adequately exploits the second-order advantage which enables quantitation of analyte in the presence of unknown and uncalibrated interferences. The procedure simultaneously takes into account the spectroscopic and pH-dependent properties of the compounds, which leads to a higher selectivity. Specially, for CAF determination fully protonated or deprotonated forms of CAF are not dominant in the pH range of data acquisition but spectral changes with pH were recorded and used for accurate determination of CAF. Furthermore, quantitative determination of an analyte in a complex mixture is performed using a synthetic solution as standard containing only the analyte of interest. Even in the presence of the rank deficiencies, in most cases accurate quantitation with relative errors in prediction lower than 5 % was obtained. The procedure was successfully applied to the analysis of real samples (pharmaceuticals) using synthetic external standards. Percent relative errors of 4.03, 3.26, and 5.85 were obtained for ASA, PAR, and CAF, respectively, in A.C.A tablets and 4.49 and 2.75 for PAR and CAF, respectively in Novafen capsules. 相似文献
17.
A general strategy for the study of degradation processes of drugs based on stopped-flow monitoring in a flow system is proposed. The flow system consists of a two-channel manifold for pumping sample and buffer solutions, which join and mix in a PTFE coil (57 cm × 0.7 mm i.d). The flow is stopped when the sample reaches the detection cell and, then, the corresponding kinetic processes are monitored in the spectral range 200-300 nm using a UV-vis diode array spectrophotometer. 5-Azacytidine has been chosen as a model of unstable drugs to illustrate the possibilities of the procedure. Kinetic runs have been developed at temperatures in the range 25-80 °C and pH values from 2 to 11 in order to investigate the influence of these factors on the degradation of the pharmaceutical agent. Multivariate curve resolution based on alternating least squares has been used for the data treatment in order to obtain the kinetic and spectral profiles of species involved in the degradation as well as to calculate the kinetic constants. Results indicate that 5-azacytidine is moderately stable in acid solutions while quickly decomposes in alkaline media. In addition, the degradation is dramatically accelerated with increasing temperature. 相似文献
18.
Self-modeling curve resolution (SMCR) methods, simple-to-use interactive self-modeling mixture analysis (SIMPLISMA) and alternating least squares (ALS) were used to calculate pure concentration profiles and pure spectra for the two-way spectral data collected during the on-line polycondensation reaction of bis(hydroxyethylterephthalate) with an ATR-FT-IR spectrometer. In order to improve the resolution results, SIMPLISMA was combined with local rank analysis method, fixed size moving window evolving factor analysis (FSMWEFA) to search for selective regions of various components and then look for the purest wavenumber variables in the selective regions. Such combination allows more accurate determination of the number of chemical components in the reaction system and the calculations of more accurate concentration profiles and spectra. 相似文献
19.
Multivariate curve resolution-alternating least squares (MCR-ALS) is shown to be a powerful tool to resolve coelution problems in liquid chromatograpy-mass spectrometry (LC-MS) in scan mode. This investigation was performed using two types of LC columns, one traditional LC column of 25 cm length with a slow gradient and a shorter LC column of 7.5 cm with a rapid gradient which allowed much faster analysis and save of reagents and solvents. Mixtures of multiple biocide compounds were simultaneously analyzed in standard mixtures and in environmental samples (sediment and wastewater samples) with little sample pretreatment. Using the more traditional LC 25 cm column, all biocide compounds were properly resolved by MCR-ALS and quantitatively analyzed with estimated errors always below 20%. When fast chromatography (LC column of 7.5 cm) was used, MCR-ALS resolution of the more strongly coeluted compounds was also achieved but limitations were found in their simultaneous quantitative determination, specially for environmental samples. 相似文献
20.
R. Klement F. Stock H. Elias H. Paulus P. Pelikn M. Valko M. Mazúr 《Polyhedron》1999,18(27):1356-3628
Salen type complexes, CuL, the corresponding tetrahydrosalen type complexes, Cu[H4]L, and N,N′-dimethylated tetrahydrosalen type complexes, Cu[H2Me2]L, were investigated using cyclic voltammetry, and electronic and ESR spectroscopy. In addition, the analogous copper(II) complexes with a derivative of the tetradentate ligand ‘salphen’ [salphen=H2salphen=N,N′-disalicylidene-1,2-diaminobenzene] were studied. Solutions of CuL, Cu[H4]L and Cu[H2Me2]L are air-stable at ambient temperature, except for the complex Cu(tBu, Me)[H4]salphen [H2(tBu, Me)[H4]salphen=N,N′-bis(2-hydroxy-3-tert-butyl-5-methylbenzyl)-1,2-diaminobenzene]. Cu(tBu, Me)[H4]salphen interacts with dioxygen and the ligand is oxidatively dehydrogenated (–CH2–NH–→–C=N–) to form Cu(tBu, Me)[H2]salphen and finally, in the presence of base, Cu(tBu, Me)salphen. X-ray structure analysis of Cu(tBu, Me)[H2Me2]salen confirms a slightly tetrahedrally distorted planar geometry of the CuN2O2 coordination core. The complexes were subjected to spectrophotometric titration with pyridine, to determine the equilibrium constants for adduct formation. It was found that the metal center in the complexes studied is only of weak Lewis acidity. In dichlormethane, the oxidation Cu(II)/Cu(III) is quasireversible for the CuL type complexes, but irreversible for the Cu[H4]L and Cu[H2Me2]L type. A poorly defined wave was observed for the irreversible reduction Cu(II)/Cu(I) at potentials less than −1.0 V. The ESR spectra of CuL at both 77 K and room temperature reveal that very well resolved lines can be attributed to the interaction of an unpaired electron spin with the copper nuclear spin, 14N donor nuclei and to a distant interaction with two equivalent protons [ACu(iso)≈253 MHz, AN(iso)≈43 MHz, AN(iso)≈20 MHz]. These protons are attached to the carbon atoms adjacent to the 14N nuclei. In contrast to CuL, the number of lines in the spectra of the complexes Cu[H4]L and Cu[H2Me2]L is greatly reduced. At room temperature, only a quintet with a considerably smaller nitrogen shf splitting constant [AN(iso)≈27 MHz] is observed. Both factors, planarity and conjugation, are thus essential for the observation of distant hydrogen shf splitting in CuL. Due to the C=N bond hydrogenation, the coordination polyhedra of the complexes Cu[H4]L and Cu[H2Me2]L is more flexible and more sensitive to ligand modification than that of CuL. The electron-withdrawing effect of the phenyl ring of the phenylenediamine bridge is reflected in a reduction of the copper hyperfine coupling constants in Cu(tBu, Me)[H4]salphen and Cu(tBu, Me)[H2Me2]salphen complexes [ACu(iso)≈215 MHz]. 相似文献