共查询到20条相似文献,搜索用时 15 毫秒
1.
Roheena Abdullah Wajeeha Zafar Muhammad Nadeem Mehwish Iqtedar Shagufta Naz Quratulain Syed 《Natural product research》2015,29(12):1133-1138
This study deals with optimisation of cultural conditions for enhanced production of cellulase by Bacillus PC-BC6 and its mutant derivative Bacillus N3. Influence of different variables including incubation time, temperature, inoculum size, pH, nitrogen sources and metal ions has been studied. The optimum conditions for cellulase production were incubation period of 72 h, inoculum size 4% incubation temperature 37°C, pH 7, 0.25% ammonium sulphate, 0.2% peptone as inorganic and organic nitrogen source in case of Bacillus PC-BC6. In case of mutant Bacillus N3, optimal conditions were incubation period of 48 h, incubation temperature 37°C, inoculum size 3%, pH 7, 0.2% ammonium chloride and 0.15% yeast extract. Presence of MnSO4 and CaCl2 enhances the enzyme production by Bacillus PC-BC6 and mutant Bacillus N3, respectively. This study was innovative and successful in producing cellulase economically by using cheap indigenous substrate Saccharum spontaneum. 相似文献
2.
A three-level central composite design of the Response Surface Methodology and the Artificial Neural Network-linked Genetic
Algorithm were applied to find the optimum operating conditions for enhanced production of l-asparaginase by the submerged fermentation of Aspergillus terreus MTCC 1782. The various effects of the operating conditions, including temperature, pH, inoculum concentration, agitation
rate, and fermentation time on the experimental production of l-asparaginase, were fitted to a second-order polynomial model and non-linear models using Response Surface Methodology and
the Artificial Neural Network, respectively. The Artificial Neural Network model fitted well, achieving a higher coefficient
of determination (R
2 = 0.999) than the second-order polynomial model (R
2 = 0.962). The l-asparaginase activity (38.57 IU s mL−1) predicted under the optimum conditions of 32.08°C, pH of 5.85, inoculum concentration of 1 vol. %, agitation rate of 123.5
min−1, and fermentation time of 55.1 h was obtained using the Artificial Neural Networklinked Genetic Algorithm in very close agreement
with the activity of 37.84 IU mL−1 achieved in confirmation experiments. 相似文献
3.
Sanjay Kumar Venkata Dasu Veeranki Kannan Pakshirajan 《Applied biochemistry and biotechnology》2011,163(3):327-337
Statistically based experimental design was applied to maximize the production of glutaminase-free L-asparaginase from Pectobacterium carotovorum MTCC 1428. The effect of physical process parameters (initial pH of the medium, temperature, rpm of the shaking incubator,
and inoculum size) on the production of L-asparaginase from P. carotovorum MTCC 1428 was studied using central composite design technique. The individual optimum levels of initial pH of the medium,
temperature, rpm of shaking incubator, and inoculum size were found to be 6.90, 29.8 °C, 157 rpm, and 2.61% (v/v), respectively, for the production of L-asparaginase. After physical process parameters optimization, the production and
productivity of L-asparaginase was enhanced by 26.39% (specific activity) and 10.19%, respectively. Maximization of L-asparaginase
production was achieved at 12 h under optimal levels of physical process parameters in shake flask level. 相似文献
4.
Ramadhar Kumar S. Balaji T. S. Uma A. B. Mandal P. K. Sehgal 《Applied biochemistry and biotechnology》2010,160(1):30-39
A Bacillus subtilis (MTCC9102) isolate was shown to produce significant amount of keratinase under optimized conditions in solid-state fermentation
using Horn meal as a substrate. Optimized value for moisture, inoculum, and aeration were found to be 100% (v/w), 50% (v/w), and 150% (w/w), respectively, and the optimum nitrogen source was peptone and carbon source was dextrose. Maximum keratinolytic activity
was observed at 48 h after incubation, and the optimum age (24 h) of inoculum was significant. The influence of cultivation
temperature and initial pH of the medium on keratinase production revealed the optimum values for the temperature and pH as
37 °C and 7, respectively. Maximum keratinase activity of the crude extract was 15,972 U/mg/ml. These results indicate that
this bacterial strain shows a high biotechnological potential for keratinase production in solid-state fermentation, and use
of the horn meal as the substrate can be implemented for keratinous solid wastes management. 相似文献
5.
Dominic Agyei Ravichandra Potumarthi Michael K. Danquah 《Applied biochemistry and biotechnology》2012,168(5):1035-1050
Using a combination of conventional sequential techniques, the batch growth conditions for the production of cell-envelope-associated proteinases have for the first time been studied and optimised in Lactobacillus delbrueckii subsp. lactis 313 (ATCC 7830; LDL 313). Concentrations of inoculum (0.1?<?X?<?10?% vol/vol), agitation speed (0?<?S?<?200?rpm), varying incubation temperature (30?<?T?<?50?°C), starting pH (4.5?<?pH?<?7) and carbon/nitrogen ratio of production medium (0.2?<?r?<?5) had an individual effect on proteinase yield (p?<?0.01). Optimal conditions for proteinase production included an initial pH of 6.0, 45?°C incubation temperature, 2?% (v/v) inoculum size of OD560?=?1, 150?rpm agitation speed, and growth medium carbon/nitrogen ratio of 1.0. Maximum proteinase activity obtained for whole cells was 0.99 U/ml after 8?h of incubation. The variables studied are very relevant due to their significance in improving the productivity of proteinase synthesis from LDL 313, under process and, likely, economic optimum conditions. 相似文献
6.
The effects of different aeration conditions on xylitol production from corncob hemicellulose hydrolysate by Candida sp. ZU04 were investigated. Batch fermentations were carried out in a 3.7-L fermentor at 30°C, pH5.5, and agitation of 300
rpm. It was found that the two-phase aeration process was more effective than the one-phase aeration process in xylitol production.
In the first 24h of the aerobic phase, a high aeration rate was applied, glucose was soon consumed, and biomass increased
quickly. In the second fermentation phase, aeration rate was reduced and an improved xylitol yield was obtained. The maximum
xylitol yield (0.76 g/g) was obtained with an aeration rate of 1.5 vvm (KLa of 37 h−1) for the first 24 h and 0.3 vvm (KLa of 6 h−1) from 24 to 96 h. 相似文献
7.
Kinetics of Growth and Enhanced Sophorolipids Production by Candida bombicola Using a Low-Cost Fermentative Medium 总被引:1,自引:0,他引:1
In this study, effect of various parameters on sophorolipid (SL) production by the yeast Candida bombicola was investigated for the enhancing of its production by employing L18 orthogonal array design of experiments. At optimum
conditions of sugarcane molasses 50 g l−1, soybean oil 50 g l−1, inoculum size 5% (v/v), temperature 30 °C, inoculum age 2 days, and agitation 200 rpm, the yeast produced almost equal amounts of the product in
batch shake flasks and in a 3-l fermentor without any pH control (45 and 47 g l−1, respectively). However, the yield increased to 60 g l−1 in the fermentor under controlled pH environment. Time course of SL production, yeast biomass growth, and utilization of
sugarcane molasses and soybean oil at these optimized conditions were fitted to existing kinetic models reported in the literature.
Estimated kinetic parameters from these models suggested that conventional medium containing glucose can very well be replaced
with the present low-cost fermentative medium. 相似文献
8.
Assamoi AA Destain J Delvigne F Lognay G Thonart P 《Applied biochemistry and biotechnology》2008,145(1-3):87-98
Xylanase is produced by Penicillium canescens 10–10c from soya oil cake in static conditions using solid-state fermentation. The impact of several parameters such as the nature
and the size of inoculum, bed-loading, and aeration is evaluated during the fermentation process. Mycelial inoculum gives
more production than conidial inoculum. Increasing the quantity of inoculum enhances slightly xylanase production. Forced
aeration induces more sporulation of strain and reduces xylanase production. However, forced moistened air improves the production
compared to production obtained with forced dry air. In addition, increasing bed-loading reduces the specific xylanase production
likely due to the incapacity of the Penicillium strain to grow deeply in the fermented soya oil cake mass. Thus, the best cultivation conditions involve mycelial inoculum
form, a bed loading of 1-cm height and passive aeration. The maximum xylanase activity is obtained after 7 days of fermentation
and attains 10,200 U/g of soya oil cake. These levels are higher than those presented in the literature and, therefore, show
all the potentialities of this stock and this technique for the production of xylanase. 相似文献
9.
Damaso Mônica C. T. Andrade Carolina M. M. C. Pereira Nei 《Applied biochemistry and biotechnology》2000,84(1-9):821-834
The production of cellulase-free end oxylanase by the thermophilic fungus Thermomyces lanuginosus was investigated insemisolid fermentation and liquid fermentation. Different process variables were investigated in semisolid
fermentation, employing corncobas the carbon source. The best results were with the following conditions: grain size=4.5 mm,
solid:liquid ratio=1:2, and inoculum size=20% (v/v). Corncob, xylan, and xylose were the best inducers for endoxylanase production.
Additionally, organic nitrogen sources were necessary for the production of high endoxylanase activities. The crude enzyme
had optimum activity at pH 6.0 and 75°C, displaying a high thermostability. The apparent K
25 and V
max were 1.77 mg of xylan/mL and 21.5 U/mg of protein, respectively. 相似文献
10.
Majid Afshari Fakhri Shahidi Seyed Ali Mortazavi Farideh Tabatabai Zarin Es'haghi 《Natural product research》2015,29(14):1300-1306
In this study, the combined effect of pH, temperature and agitation speed on yellow pigment production and mycelial growth of Penicillium aculeatum ATCC 10409 was investigated in whey media. Different pH levels (5, 6.5 and 8), temperatures (25, 30 and 35°C) and agitation speed levels (100 and 150 rpm) were tested to determine the best conditions to produce a fungal yellow pigment under submerged fermentation. The best production of yellow pigment (1.38 g/L) was obtained with a pH value of 6.5, a temperature of 30°C and an agitation speed of 150 rpm. In contrast, the maximal biomass concentration (11.12 g/L) was obtained at pH value of 8, a temperature of 30°C and an agitation speed of 100 rpm. These results demonstrated that biomass and yellow pigment production were not directly associated. The identification of the structure of unknown P. aculeatum yellow pigment was detected using UV absorption spectrum and FT-IR spectroscopy. 相似文献
11.
Pryor SW Gibson DM Hay AG Gossett JM Walker LP 《Applied biochemistry and biotechnology》2007,143(1):63-79
Bacillus subtilis strain TrigoCor 1448 was grown on wheat middlings in 0.5-l solid-state fermentation (SSF) bioreactors for the production
of an antifungal biological control agent. Total antifungal activity was quantified using a 96-well microplate bioassay against
the plant pathogen Fusarium oxysporum f. sp. melonis. The experimental design for process optimization consisted of a 26−1 fractional factorial design followed by a central composite face-centered design. Initial SSF parameters included in the
optimization were aeration, fermentation length, pH buffering, peptone addition, nitrate addition, and incubator temperature.
Central composite face-centered design parameters included incubator temperature, aeration rate, and initial moisture content
(MC). Optimized fermentation conditions were determined with response surface models fitted for both spore concentration and
activity of biological control product extracts. Models showed that activity measurements and spore production were most sensitive
to substrate MC with highest levels of each response variable occurring at maximum moisture levels. Whereas maximum antifungal
activity was seen in a limited area of the design space, spore production was fairly robust with near maximum levels occurring
over a wider range of fermentation conditions. Optimization resulted in a 55% increase in inhibition and a 40% increase in
spore production over nonoptimized conditions. 相似文献
12.
Vandana Saini Amrik Bhattacharya Anshu Gupta 《Applied biochemistry and biotechnology》2013,170(8):1896-1908
The present study is an attempt to demonstrate the feasibility of sal (Shorea robusta) deoiled cake—a forest-based industrial by-product—as a cheaper media supplement for augmented protease production from Aeromonas sp. S1 and application of protease in the treatment of kitchen wastewater. Under optimized conditions, protease production could successfully be enhanced to 5.13-fold (527.5 U mL?1) on using sal deoiled seed cake extract (SDOCE), as medium additive, compared to an initial production of 102.7 U mL?1 in its absence. The culture parameters for optimum production of protease were determined to be incubation time (48 h), pH (7.0), SDOCE concentration (3 % (v/v)), inoculum size (0.3–0.6 % (v/v)), and agitation rate (100 rpm). The enzyme was found to have an optimum pH and temperature of 8.0 and 60 °C, respectively. The protease preparation was tested for treatment of organic-laden kitchen wastewater. After 96 h of wastewater treatment under static condition, enzyme preparation was able to reduce 74 % biological oxygen demand, 37 % total suspended solids, and 41 % oil and grease. The higher and improved level of protease obtained using sal deoiled seed cake-based media hence offers a new approach for value addition to this underutilized biomass through industrial enzyme production. The protease produced using this biomass could also be used as pretreatment tool for remediation of organic-rich food wastewater. 相似文献
13.
Fernanda Germano Alves Francisco Maugeri Filho Janaína Fernandes de Medeiros Burkert Susana Juliano Kalil 《Applied biochemistry and biotechnology》2010,160(5):1528-1539
In this work, the agitation and aeration effects in the maximization of the β-galactosidase production from Kluyveromyces marxianus CCT 7082 were investigated simultaneously, in relation to the volumetric enzyme activity and the productivity, as well as
the analysis of the lactose consumption and production of glucose, and galactose of this process. Agitation and aeration effects
were studied in a 2 L batch stirred reactor. A central composite design (22 trials plus three central points) was carried out. Agitation speed varied from 200 to 500 rpm and aeration rate from 0.5
to 1.5 vvm. It has been shown in this study that the volumetric enzyme production was strongly influenced by mixing conditions,
while aeration was shown to be less significant. Linear models for activity and productivity due to agitation and aeration
were obtained. The favorable condition was 500 rpm and 1.5 vvm, which lead to the best production of 17 U mL−1 for enzymatic activity, 1.2 U mL−1 h−1 for productivity in 14 h of process, a cellular concentration of 11 mg mL−1, and a 167.2 h−1 volumetric oxygen transfer coefficient. 相似文献
14.
O. García-Kirchner M. Segura-Granados P. Rodríguez-Pascual 《Applied biochemistry and biotechnology》2005,121(1-3):347-359
The hydrolytic activity of fungal originated β-glucosidase is exploited in several biotechnological processes to increase
the rate and extent of saccharification of several cellulosic materials by hydrolyzing the cellobiose which inhibits cellulases.
In a previous presentation, we reported the screening and liquid fermentation with Aspergillus niger, strain C-6 for β-glucosidase production at shake flask cultures in a basal culture medium with mineral salts, corn syrup liquor, and
different waste lignocellulosic materials as the sole carbon source obtaining the maximum enzymatic activity after 5–6 d of
8.5 IU/mL using native sugar cane bagasse. In this work we describe the evaluation of fermentation conditions: growth temperature,
medium composition, and pH, also the agitation and aeration effects for β-glucosidase production under submerged culture using
a culture media with corn syrup liquor (CSL) and native sugar cane bagasse pith as the sole carbon source in a laboratory
fermenter. The maximum enzyme titer of 7.2 IU/mL was obtained within 3 d of fermentation. This indicates that β-glucosidase
productivity by Aspergillus niger
C-6 is function of culture conditions, principally temperature, pH, culture medium conditions, and the oxygen supply given in
the bioreactor. Results obtained suggest that this strain is a potential microorganism that can reach a major level of enzyme
production and also for enzyme characterization. 相似文献
15.
Vandenberghe LP Soccol CR Prado FC Pandey A 《Applied biochemistry and biotechnology》2004,118(1-3):293-303
Studies were conducted to evaluate citric acid production by solid-state fermentation (SSF) using cassava bagasse as substrate
employing a fungal culture of Aspergillus niger LPB 21 at laboratory and semipilot scale. Optimization of the process parameters temperature, pH, initial humidity, aeration,
and nutritive composition was conducted in flasks and column fermentors. The results showed that thermal treatment of cassava
bagasse enhanced fungal fermentation efficacy, resulting in 220 g of citric acid/kg of dry cassava bagasse with only treated
cassava bagasse as substrate. The results obtained from the factorial experimental design in a column bioreactor showed that
an aeration rate of 60 mL/min (3 mL/[g·min]) and 60% initial humidity were optimum, resulting in 265.7 g/kg of dry cassava
bagasse citric acid production. This was almost 1.6 times higher than the quantities produced under unoptimized conditions
(167.4 g of citric acid/kg of dry cassava bagasse). The defined parameters were transferred to semipilot scale, which showed
high promise for large-scale citric acid production by SSF with cassava bagasse. Respirometry assays were carried out in order
to follow indirectly the biomass evolution of the process. Citric acid production reached 220, 309, 263, and 269 g/kg of dry
cassava bagasse in Erlenmeyer flasks, column fermentors, a tray bioreactor, and a horizontal drum bioreactor, respectively. 相似文献
16.
Zhengshan Luo Li Chen Changhong Chen Wei Zhang Ming Liu Ye Han Jiangang Zhou 《Applied biochemistry and biotechnology》2014,172(3):1282-1292
The production and characterization of a bioflocculant, MBF-6, by Klebsiella pneumoniae was investigated. Optimum culture conditions for bioflocculant production were an initial medium pH of 7, an incubation temperature of 30 °C, and an inoculum size of 1 % (v/v) of cell density 1.0?×?108 cfu/mL. The carbon, nitrogen, and cation sources for optimum bioflocculant production were glucose, peptone, and ZnCl2. The bioflocculant mainly consisted of protein (3.4 %) and sugar (95.1 %). Fourier transform infrared (FTIR) spectrum revealed the presence of carboxyl and hydroxyl groups while the thermogravimetric analysis (TGA) showed a degradation temperature (T d) of 81.4 °C. MBF-6 had a good flocculating rate in kaolin suspension without cation addition and was stable over a wide range of pH and temperature. Investigation on the flocculation efficacy of the characterized MBF-6 for wastewater treatment of dairy, woolen, brewery, and sugar industries suggested it to be effective. 相似文献
17.
Yoshiharu Yamane Yozo Nakamura Hiroyuki Okamoto Hiroshi Ooshima Jyoji Kato 《Applied biochemistry and biotechnology》1995,50(3):317-322
Effects of pH and dissolved oxygen concentration on batchwise riboflavin production by a 5-fluorouracil (5-FU)-resistant mutant
ofArthrobacter sp. were investigated. The reaction was carried out in a jar fermentor. The optimal pH of culture medium was around 7.3.
Dissolved oxygen concentration was almost constant during fermentation at 600 rpm of agitation rate. Production of riboflavin
reached a maximum of 160 mg/L after 70 h fermentation under the agitation rate of 600 rpm, aeration rate of 1.0 L/min, and
pH 7.0. 相似文献
18.
Flávia Pereira Duta Francisca Pessôa De França Eliana Flávia Camporese Sérvulo Léa Maria De Almeida Lopes Antonio Carlos Augusto Da Costa Ana Barros 《Applied biochemistry and biotechnology》2004,114(1-3):639-652
The production of biopolymers by a Rhizobium strain was studied under batch and bioreactor conditions. The best viscosity levels were obtained under low mannitol concentrations as well as low agitation and aeration conditions. Infrared spectra indicated the presence of chemical groups characteristic of microbially produced biopolymers, including C=O and O-acetyl groups. Thermogravimetric analysis showed the characteristic degradation profiles of the exopolysaccharide produced (T onset=290°C). The experimental design showed that a low substrate concentration (10.0 g/L), and low aeration (0.2 vvm) and agitation (200 rpm) levels should be used. The maximum yield of the process was a Yp/s (g/g) of 0.19±0.1, obtained under optimized conditions. 相似文献
19.
Optimization of Surfactin Production by <Emphasis Type="Italic">Bacillus subtilis</Emphasis> Isolate BS5 总被引:1,自引:0,他引:1
Abdel-Mawgoud AM Aboulwafa MM Hassouna NA 《Applied biochemistry and biotechnology》2008,150(3):305-325
Bacillus subtilis BS5 is a soil isolate that produces promising yield of surfactin biosurfactant in mineral salts medium (MSM). It was found that cellular growth and surfactin production in MSM were greatly affected by the environmental fermentation conditions and the medium components (carbon and nitrogen sources and minerals). Optimum environmental conditions for high surfactin production on the shake flask level were found to be a slightly acidic initial pH (6.5-6.8), an incubation temperature of 30 degrees C, a 90% volumetric aeration percentage, and an inoculum size of 2% v/v. For media components, it was found that the optimum carbon source was molasses (160 ml/l), whereas the optimum nitrogen source was NaNO(3) (5 g/l) and the optimum trace elements were ZnSO(4).7H(2)O (0.16 g/l), FeCl(3).6H(2)O (0.27 g/l), and MnSO(4).H(2)O (0.017 g/l). A modified MSM (molasses MSM), combining the optimum medium components, was formulated and resulted in threefold increase in surfactin productivity that reached 1.12 g/l. No plasmid could be detected in the tested isolate, revealing that biosurfactant production by B. subtilis isolate BS5 is chromosomally mediated but not plasmid-mediated. 相似文献
20.
Among physical and nutritional parameters optimized by “one variable at a time” approach, four cultural variables (sucrose,
MgSO4
.7H2O, inoculum size, and incubation period) significantly affected glucoamylase production. These variables were, therefore,
selected for optimization using response surface methodology. The p-values of the coefficients for linear effect of sucrose and inoculum size were less than 0.0001, suggesting them to be the
key experimental variables in glucoamylase production. The enzyme production (34 U/ml) attained under optimized conditions
(sucrose, 2%; MgSO4
.7H2O, 0.13%; yeast extract, 0.1%; inoculum size, 5 × 106 spores per 50 ml production medium; incubation time, 48 h; temperature, 40°C; and pH 7.0) was comparable with the value predicted
by polynomial model (34.2 U/ml). An over all 3.1-fold higher enzyme titers were attained due to response surface optimization.
The experimental model was validated by carrying out glucoamylase production in shake flasks of increasing capacity (0.25–2.0 l)
and 22-l laboratory bioreactors (stirred tank and airlift), where the enzyme production was sustainable. Furthermore, the
fermentation time was reduced from 48 h in shake flasks to 32 h in bioreactors. 相似文献