共查询到17条相似文献,搜索用时 187 毫秒
1.
2.
同步荧光法测定复方新诺明中磺胺甲基异恶唑和甲氧苄氨嘧啶的含量… 总被引:5,自引:0,他引:5
本文采用同步荧光技术对复方新诺明中有效成份磺胺甲基异恶唑和甲氧苄氨嘧啶进行测定,在△λ分别为72nm和94nm的条件下,测定样品中所含有效成份相当于标示量的95.12%-105.68%;回收率为95.5%-98.1%;线性范围磺胺甲基异恶唑为0.125-4.00μg/ml。甲氧苄氨嘧啶为0.125-3.00μg/ml;相关系数分别为0.9976和0.9968。不经分离和掩蔽直接连续测定,效果良好。 相似文献
3.
4.
5.
报道了利用30mmol/LHAc-NaAc为缓冲液测定复方新诺明中甲氧苄胺嘧啶和磺胺甲基异恶唑的离解常数及含量的毛细管区带电泳方法。该方法具有简便、快速、准确等特点。测定TMP和SMZ的PKa分别为6.60和5.90。其平均回收率分别为101.5%和99.6%最低检出限为0.48mg/L和0.028mg/L,RSD为1.26%和1.12%。 相似文献
6.
7.
8.
HPLC法同时测定复方新诺明注射液中SMZ和TMP的含量 总被引:2,自引:0,他引:2
生产上复方新诺明注射液中甲氧苄氨嘧啶(TMP) 的含量采用氯仿提取后用紫外分光光度法测定,该法操作繁复,且溶剂毒性较大。作者研究了用HPLC法同时测定磺胺E甲唑(SMZ)和TMP含量的方法,15min内可完成测定,本法简便方便,重现性好。 相似文献
9.
分光光度法测定水中痕量甲萘胺 总被引:1,自引:2,他引:1
提出分光光度法测定水中痕量中甲萘胺的新方法,甲萘胺与对硝基重氮盐偶合成黄色的染料,在475nm处进行分光光度法测定,甲萘胺的浓度在1.0~20.0mg/L时呈线性,检出限为1mg/L,回收率在95.4%~105.2%,该法具有灵敏度高,对甲萘胺有较好的选择性等特点,用于水中痕量甲萘胺的测定,结果令人满意。 相似文献
10.
11.
12.
13.
被分析组分的光谱净信号将正交于其它药物组分和辅料光谱所张成的光谱子空间,从而消除其它组分对被分析组分的光谱干扰,此净信号将与被分析组分的浓度成正比,据此建立了药物制剂分析的净信号方法。本文利用该方法研究了在酸性条件下紫外光谱测定复方磺胺甲噁唑片的两种有效成分磺胺甲噁唑(SMZ)和甲氧苄啶(TMP)。SMZ和TMP的线性范围分别为0.48~7.84μg/mL(r=0.9981)和0.12~1.5μg/mL(r=0.9986),平均回收率分别为99.5%和101.0%,RSD分别为1.87%和3.60%。本法简便、快速和准确,可用于酸性条件下复方磺胺甲噁唑片中SMZ和TMP含量的快速、同时准确测定。 相似文献
14.
15.
Givianrad MH Saber-Tehrani M Aberoomand-Azar P Mohagheghian M 《Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy》2011,78(3):1196-1200
The applicability of H-point standard additions method (HPSAM) to the resolving of overlapping spectra corresponding to the sulfamethoxazole and trimethoprim is verified by UV-vis spectrophotometry. The results show that the H-point standard additions method with simultaneous addition of both analytes is suitable for the simultaneous determination of sulfamethoxazole and trimethoprim in aqueous media. The results of applying the H-point standard additions method showed that the two drugs could be determined simultaneously with the concentration ratios of sulfamethoxazole to trimethoprim varying from 1:18 to 16:1 in the mixed samples. Also, the limits of detections were 0.58 and 0.37 μmol L(-1) for sulfamethoxazole and trimethoprim, respectively. In addition the means of the calculated RSD (%) were 1.63 and 2.01 for SMX and TMP, respectively in synthetic mixtures. The proposed method has been successfully applied to the simultaneous determination of sulfamethoxazole and trimethoprim in some synthetic, pharmaceutical formulation and biological fluid samples. 相似文献
16.
A capillary electrophoretic method for the simultaneous determination of sulfamethoxazole and trimethoprim in plasma was developed. Sulfamethoxazole and trimethoprim extracted from human plasma with ethyl acetate were analyzed at 20 kV and 25 degrees C using 15 mm phosphate buffer (pH 6.2) as the electrolyte. The detection was by UV at 220 nm. The run time was 8.0 min and the limit of quantification was 10.00 microg/mL for sulfamethoxazole and 2.00 microg/mL for trimethoprim. The recovery was >99% for both compounds. This method enabled the detection of sulfamethoxazole and trimethoprim in plasma of patients after oral ingestion of their combined formulation. The present simple and rapid method is applicable to drug monitoring in immunocompromised patients who are taking the combined formulation of these compounds for the treatment or prophylaxis of Pneumocystis carinii pneumonia. 相似文献
17.
用统计模拟分光光度法测定复方消咳新片4个组分含量。按均匀设计表制备合成样品,绘制溶液的UV-VIS吸收曲线,获得有限但足够的实验数据,用逐步回归法构造反映该复方制剂组分在灵敏波长的吸光度-组分含量经验关系的“最优”数学模型,用改进单纯形法寻优,求出未知样品的各组分含量。磺胺甲基异恶唑、甲氧苄胺嘧啶、盐酸溴已新、枸橼酸维静宁的回收率分别为1003%、1004%、999%、990%,标准差分别为091%、14%、12%、39%。 相似文献