以马来松香丙烯酸乙二醇酯为交联剂的茶碱分子印迹膜电化学传感器的研究

利用分子印迹技术, 以马来松香丙烯酸乙二醇酯为交联剂, 丙烯酸为功能单体研制了测定茶碱的新型电化学传感器. 以自由基热聚合的方式于玻碳电极表面制备茶碱分子印迹聚合物膜. 通过循环伏安法、电化学交流阻抗法及计时电流法研究了传感器对茶碱的响应特性. 结果表明, 在最佳的实验条件下, 传感器的峰电流与茶碱浓度在2.00×10^-7～3.45×10^-4 mol/L 范围内呈现出良好的线性关系(线性相关系数为0.9961), 检出限(S/N＝3)为1.00×10^-7 mol/L;该传感器的选择性高, 稳定性和重现性好, 将此传感器用于茶碱缓释片中茶碱的含量测定, 回收率为95.6%～103.8%.关键词 马来松香丙烯酸乙二醇酯; 茶碱; 分子印迹聚合物; 电化学传感器     

葛根素分子印迹电化学传感器的制备及研究

以丙烯酰胺为功能单体,葛根素为模板分子,马来松香丙烯酸乙二醇酯为交联剂,采用循环伏安法合成了葛根素分子印迹膜,并以此为识别元件制备了葛根素电化学传感器。该传感器对葛根素具有高度的选择性和良好的敏感度,葛根素氧化峰电流与其浓度在6.0×10-8～1.6×10-3mol/L范围内呈良好的线性关系,检出限为2.0×10-8mol/L。将此传感器用于葛根素注射液和木瓜葛根片中葛根素的含量测定,回收率为97.7%～106.4%。     

苯巴比妥分子印迹电化学传感器的制备及其性能研究

以马来松香丙烯酸乙二醇酯为交联剂,甲基丙烯酸为功能单体,偶氮二异丁腈为引发剂,苯巴比妥为模板分子,在玻碳电极表面热聚合了一种用于测定苯巴比妥的印迹聚合物传感膜。探索了传感器的最佳实验条件,并在此实验条件下,采用循环伏安法、差分脉冲伏安法及电化学交流阻抗法研究了传感器对苯巴比妥的响应特性。实验结果表明,传感器的峰电流响应值与苯巴比妥的浓度在3.0×10-6~1.0×10-4mol/L范围内呈现良好的线性关系(线性相关系数R=0.9976),检出限(S/N=3)为5.4×10-7mol/L。用此印迹传感器对苯巴比妥片中苯巴比妥的含量进行了测定,回收率在91.5%~107.5%。     

呋喃妥因分子印迹电化学传感器的制备及应用

以甲基丙烯酸为功能单体,呋喃妥因为模板分子,马来松香丙烯酸乙二醇酯(EGMRA)为交联剂,在玻碳电极表面制备了呋喃妥因分子印迹膜。采用循环伏安(CV)法、差分脉冲伏安(DPV)法及交流阻抗(EIS)法对印迹膜进行表征。实验表明,DPV法测定的氧化峰电流与呋喃妥因浓度在8.0×10-8~5.0×10-6 mol/L范围内呈良好的线性关系(R=0.9939),检出限为6.5×10-8 mol/L。该传感器用于呋喃妥因肠溶片的测定,其回收率为96.6%~101.6%。     

基于石墨烯增敏的甲基对硫磷分子印迹电化学传感器的研制

以甲基对硫磷为模板分子,甲基丙烯酸为功能单体,马来松香丙烯酸乙二醇酯为交联剂,在石墨烯修饰的玻碳电极表面合成分子印迹聚合物膜,制备分子印迹电化学传感器,用于检测有机磷农药甲基对硫磷。采用循环伏安法、微分脉冲伏安法、电化学交流阻抗法研究传感器的电化学特性及分析特性。结果表明,在最佳检测条件下,传感器的峰电流与甲基对硫磷浓度在1.0×10^-7～8.0×10^-5 mol/L范围内呈线性关系,线性方程为I_p(μA)=-0.65199-0.02387C(μmol/L),相关系数R²为0.9922,检出限为5.0×10^-8 mol/L(S/N=3)。构建了传感器的动力学吸附模型,测得印迹传感器的结合速率常数k=20.2758s。将此传感器用于番茄样品中甲基对硫磷的检测,加标回收率为95.0%～100.3%。     

基于石墨烯的毒死蜱分子印迹电化学传感器的制备及对毒死蜱的测定

利用分子印迹技术,以马来松香丙烯酸乙二醇酯为交联剂,使用自由基热聚合法在石墨烯修饰的玻碳电极表面合成毒死蜱( CPF)分子印迹聚合膜,制得了CPF分子印迹电化学传感器。采用循环伏安法、线性扫描伏安法和电化学交流阻抗法等,考察了此CPF分子印迹膜的电化学性能。在最佳检测条件下,传感器的峰电流与CPF浓度在2.0×10-7~1.0×10-5mol/L范围内呈线性关系,线性方程为Ip(μA)=-7.1834-0.2424C (μmol/L),相关系数r2=0.9959,检出限为6.7×10-8 mol/L(S/N=3)。构建了CPF分子印迹电化学传感器的动力学吸附模型,测得印迹传感器的印迹因子β=2.59,结合速率常数k=12.2324 s。传感器表现出良好的重现性和稳定性,并成功用于实际水样和蔬菜样品中CPF的测定,加标回收率为94.1%~101.4%。     

基于电聚合的苯巴比妥分子印迹电化学传感器的制备及其应用研究

以马来松香丙烯酸乙二醇酯(EGMRA)为交联剂,甲基丙烯酸为功能单体,在玻碳电极表面电聚合了一种对苯巴比妥分子具有专一性识别的聚合膜。在最佳实验条件下,采用循环伏安法(CV)、差分脉冲伏安法(DPV)及电化学交流阻抗法(EIS)对印迹传感器的性能进行研究。实验结果表明:该印迹传感器对苯巴比妥具有快速响应、专一性识别和高灵敏度的特点,且苯巴比妥的浓度在8.0×10-7~1.0×10-4mol/L范围内与DPV电流信号呈良好的线性关系,相关系数(r)为0.998 3。检出限(S/N=3)为5.4×10-8mol/L。将此印迹传感器应用于实际样品中苯巴比妥的检测,加标回收率为95.7%~105.0%。     

基于石墨烯掺杂金纳米粒子的速灭威分子印迹电化学传感器

以速灭威(MTMC)为模板分子,甲基丙烯酸(MAA)为功能单体,马来松香丙烯酸乙二醇酯(EGMRA)为交联剂,在石墨烯掺杂金纳米粒子修饰玻碳电极表面合成分子印迹膜,研制了测定MTMC的分子印迹电化学传感器。采用扫描电镜(SEM)对传感膜的形貌进行了表征,通过循环伏安法(CV)、电化学阻抗谱法(EIS)和差示脉冲伏安法(DPV)对传感器的性能进行了研究。DPV测试表明,MTMC的浓度在1.0×10-7~1.0×10-4mol/L范围内呈现良好的线性关系(线性相关系数为R=0.9936),检出限2.9×10-8mol/L(S/N=3)。传感器应用于蔬菜样品的加标回收检测,回收率在93.4%~106.4%之间。     

纳米氧化铜掺杂的苯巴比妥分子印迹电化学传感器的制备及其识别性能研究

为了提高苯巴比妥分子印迹传感器的灵敏度,以甲基丙烯酸为功能单体,马来松香丙烯酸乙二醇酯为交联剂,热聚合了一种纳米氧化铜掺杂的苯巴比妥分子印迹传感器。分别采用循环伏安法( CV)、电化学交流阻抗法(EIS)、差分脉冲伏安法(DPV)、计时电流法(CA)对这种印迹传感器的电化学性能进行了研究。分别采用红外光谱、扫描电镜对此印迹传感器的结构及形貌进行了表征。结果表明,以铁氰化钾为分子探针的间接检测中,铁氰化钾的峰电流值与苯巴比妥的浓度在1.2×10-7~1.5×10-4 mol/L范围内呈现良好的线性关系(线性相关系数R=0.9984),检出限(S/N=3)为8.2×10-9 mol/L。将此印迹传感器用于实际应用,回收率在96.5%~103.0%之间。     

分子印迹电化学传感器

分子印迹电化学传感器能够选择性识别并检测特定目标化合物,因其设计简单、灵敏度高、价格低廉、携带方便、易于微型化和自动化等优点,在临床诊断、环境监测、食品分析等方面越来越受到人们的关注.本文作者主要论述分子印迹技术与电化学技术相结合构建分子印迹电化学传感器,包括分子印迹电化学传感器的种类,以及电化学方法制备分子印迹聚合物膜的常用单体等.对分子印迹电化学传感器领域新出现的分子印迹聚合物-纳米材料复合物以及纳米结构分子印迹聚合物也一并做了评述.     

Development and Characterization of an Electrochemical Sensor for Cinchonidine Detection Based on Molecularly Imprinted Polymer with Modified Rosin as Cross-linker

Molecularly imprinted polymers(MIPs) were applied as molecular recognition elements to an electrochemical sensor for cinchonidine(CD). A kind of MIP was synthesized with cinchonidine as template, modified rosin( ethylene glycol maleic rosinate acrylate) containing the skeleton of phenanthrene rings as cross-linker and methylacrylic acid as functional monomer. MIP membrane was prepared on a glassy carbon electrode for the determination of CD via free radical polymerization method. Electrochemical impedance spectroscopy(EIS) and cyclic voltammetry( CV) were used to characterize the membrane electrochemical behavior in electrode fabrication process. The experimental conditions were discussed. Under optimum conditions, it was found that the response of peak currents was linear to the concentration of CD in a range of 0.013―2.26 mmol/L. The detection limit for CD is 1 μmol/L, the relative standard deviation for 100 μmol/L CD is 1.34% and the incubation time is 2 min. The sensor was applied to the determination of CD in urine samples with satisfactory results.     

吲哚-3-乙酸分子印迹聚合物膜电化学传感器的研制

利用分子印迹技术,以吲哚-3-乙酸(IAA)为模板分子,甲基丙烯酸为单体,在玻碳电极表面采用原位聚合制备分子印迹敏感膜.采用方波伏安法对吲哚乙酸在该印迹电极上的电化学行为进行了研究.结果表明,0.62 V(vs.SCE)处的峰电流与吲哚乙酸的浓度在5.0×10-6～2.0×10-4mol/L范围内呈线性关系,检出限(S...     

Graphene Doped Molecularly Imprinted Electrochemical Sensor for Uric Acid

A highly sensitive uric acid molecularly imprinted electrochemical sensor was prepared by using graphene doped chitosan as the functional matrix and uric acid as the template molecule; a electrodeposition technique was used to form a controllable graphene–chitosan–uric acid composited film on glassy carbon electrode whose uric acid was removed via electrochemical induce elution. Under the optimized preparation and detection conditions, the detection sensitivity of uric acid at graphene doped molecularly imprinted sensor was improved significantly compared with the undoped molecularly imprinted sensor. The mechanisms of sensitivity enhancement were studied by a.c. electrochemical impedance, adsorption model, and chronocoulometry. The observations suggest the effect of sensitivity enhancement resulted from magnified surface area and good electronic conduction of graphene. Additionally, the developed sensor exhibited specific recognition to uric acid against the competitors which consisted of structure liked substances and coexisting interference in blood serum.     

Molecularly Imprinted Poly-o-phenylenediamine Electrochemical Sensor for Entacapone

We have developed a molecularly imprinted polymer (MIP) electrochemical sensor for entacapone (ETC) based on an electropolymerised polyphenylenediamine (Po-PD) on a glassy carbon electrode (GCE) surface. The direct electropolymerisation of the o-phenylenediamine monomer (o-PD) was carried out with ETC as a template. The steps of electropolymerization process, template removal and binding of the analyte were tested by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) using [Fe(CN)₆]³⁻/[Fe(CN)₆]^{4 −} as a redox probe. The operation of the sensor has been investigated by differential pulse voltammetry (DPV). Under optimal experimental conditions, the response of the DPV was linearly proportional to the ETC concentration between 1.0×10⁻⁷ and 5.0×10⁻⁶ M ETC with a limit of detection (LOD) of 5.0×10⁻⁸ M. The developed sensor had excellent selectivity without detectable cross-reactivity for levodopa and carbidopa. The MIP sensor was successfully used to detect ETC in spiked human serum samples.     

盐酸阿霉素分子印迹传感器的制备及识别特性

采用自组装以及电聚合的方法,在磷酸盐缓冲液(PBS)中以3,4-乙烯二氧噻吩(EDOT)为功能单体,盐酸阿霉素(DOX)为模板,在金电极表面电聚合制备DOX印迹敏感膜(MIPs),构建了一种选择性检测DOX的分子印迹电化学传感器.采用循环伏安法(CV)及交流阻抗法(EIS)对其性能进行了表征.优化实验条件后,在含0.005 mol/L K3[Fe(CN)_6]及0.1 mol/L KCl的PBS中,应用差分脉冲伏安法(DPV)测试了该传感器的响应性能.实验结果表明,该传感器检测DOX的线性范围为4.0×10~(-7)~1.0×10~(-6)mol/L,相关系数为0.9967,检出限(S/N=3)达6.5×10~(-8)mol/L;采用电化学洗脱法可使传感器再生,对DOX的测定具有良好重现性及稳定性;该传感器对于干扰物长春碱、放线菌素D及5-氟尿嘧啶有微弱的电流响应,显示出良好的选择性.将该传感器用于人体血样中盐酸阿霉素的分析,回收率为96.0%~106.7%,表明其具有潜在的实用价值.     

分子印迹电化学传感器的研究进展

本文综述了分子印迹电化学传感器的制备及其在电分析化学领域中的应用研究。引用文献83篇。     

Capacitive Chemical Sensor for Thiopental Assay Based on Electropolymerized Molecularly Imprinted Polymer

A novel capacitive sensor based on electropolymerized molecularly imprinted polymer (MIP) for thiopental detection is described. The molecularly imprinted film as a recognition element was prepared by electropolymerization of phenol on a gold electrode in the presence of thiopental (template). Cyclic voltammetry and capacitive measurements were used for characterization and evaluation of the polymeric film. The template molecules were removed from the modified electrode surface by washing with an ethanol:water solution. The sensor’s linear response range was between 3 and 20 µM, with a detection limit of 0.6 µM. The proposed sensor exhibited good selectivity, reproducibility. Satisfactory results were obtained in the direct detection of real samples.