在金电极表面滴涂石墨烯(GR),通过电沉积技术沉积纳米金(Au)构成石墨烯-纳米金修饰电极(Au-GR/GE)。以芦丁为模板分子,邻氨基酚为功能单体,通过电聚合反应在Au-GR/GE表面合成一种对芦丁具有特异性识别能力的分子印迹传感器膜(MIP)。采用循环伏安法(CV)、差分脉冲伏安法(DPV)研究了印迹膜的性能、结构和分子印迹效应,并与槲皮素进行了选择性响应的比较,发现此传感器对芦丁具有良好的选择性。在最佳实验条件下,其对芦丁浓度的定量测定线性范围为6.30×10-7~1.70×10-4mol/L,线性方程为I(μA)=3.8136-8.6247 lg c(mol/L),R=0.9961,检出限为2.10×10-7mol/L。 相似文献
In this paper, a novel flow chemiluminescence (CL) clenbuterol sensor based on molecularly imprinted polymer (MIP) on line enrichment nanogram clenbuterol and chemiluminescence reaction of potassium permanganate and formaldehyde in the polyphosphate enhanced by clenbuterol. Clenbuterol in the urine was selectively adsorbed on the clenbuterol-imprinted polymer, which was packed into the flow cell. The formaldehyde and the polyphosphate with potassium permanganate flowed through the flow cell and reacted with the on line adsorbed clenbuterol and produced strong CL. The results show that the sensor was reversible. The CL intensity was linear with clenbuterol concentration from 1.0 × 10−9 g/mL to 5.0 × 10−8 g/mL. The detection limit was 3.0 × 10−10 g/mL. The R.S.D. for ng/mL clenbuterol was less than 5% (n = 3). The present method offered a high selectivity and sensitivity that made the quantitative analysis of trace clenbuterol (ng/mL) in the animal urine sample. 相似文献
The aim of this paper is to develop a potentiometric sensing methodology for sensitive and selective determination of neutral phenols by using a molecularly imprinted polymer as a receptor. Bisphenol A (BPA), a significant environmental contaminant, is employed as the model target. The BPA-imprinted polymer is synthesized by the semi-covalent technique and incorporated into a plasticized poly(vinyl chloride) membrane doped with the tridodecylmethylammonium salt. The present electrode shows a linear anionic potential response over the concentration range from 0.1 to 1 μM with a detection limit of 0.02 μM, and exhibits an excellent selectivity over other phenols. The proposed approach has been successfully applied to the determination of BPA released from real plastic samples. It offers promising potential in development of potentiometric sensors for measuring neutral phenols at trace levels. 相似文献
A simple electrochemical sensor based on a molecularly imprinted polymer film as the recognition element was developed for ractopamine (RAC) detection. This is the first report of a RAC-imprinted film on a gold electrode surface, synthesized through an electrochemical method using o-aminothiophenol as the functional monomer. The imprinting mechanism and experimental parameters affecting the capability of the imprinted film are discussed here. The sensor was successfully applied with constant potential amperometry for RAC detection in an indirect process with potassium ferricyanide as an electrochemical probe. The sensor had a rapid equilibrium time (120?s), high binding affinity and selectivity towards RAC, and with good reproducibility and stability. Under the experimental conditions applied, a linear relationship between the relative amperometric response and RAC ranged from 2.0?×?10(-7) to 1.4?×?10(-6)?mol?L(-1), with a lower limit of detection (LOD) of 2.38?×?10(-8)?mol?L(-1) (signal to noise ratio?=?3). The sensor was tested with feed samples spiked with trace amounts of RAC, with good recoveries between 87.4 and 90.5?%. 相似文献
A newly designed molecularly imprinted polymer (MIP) was synthesized and successfully utilized as a recognition element of an amperometric sensor for 2,4-dichlorophenol (2,4-DCP) detection. The MIP with a well-defined structure could imitate the dehalogenative function of the natural enzyme chloroperoxidase for 2,4-DCP. Imprinted sensor was fabricated in situ on a glassy carbon electrode surface by drop-coating the 2,4-DCP imprinted microgel suspension and chitosan/Nafion mixture. Under optimized conditions, the sensor showed a linear response in the range of 5.0–100 μmol L−1 with a detection limit of 1.6 μmol L−1. Additionally, the imprinted sensor demonstrated higher affinity to target 2,4-DCP over competitive chlorophenolic compounds than non-imprinted sensor. It also exhibited good stability and acceptable repeatability. The proposed sensor could be used for the determination of 2,4-DCP in water samples with the recoveries of 96.2–111.8%, showing a promising potential in practical application. 相似文献
A molecularly imprinted polymer (MIP) for the specific retention of neopterin has been developed. A set of 6 polymers was prepared by radical polymerization under different experimental condition using methacrylic acid as functional monomer and ethylene glycol dimethacrylate as crosslinker, with the aim to understand their influence on the efficiency of the MIP. The performance of each MIP was tested in batch experiments via their binding capacity. The MIP prepared in the presence of nickel ions in dimethylsulfoxide-acetonitrile mixture (P4) exhibited the highest binding capacity for neopterin (260 μmol per gram of polymer). A selectivity study with two other pteridines demonstrated the polymer P4 also to possess the best selectivity.
Figure
A molecularly imprinted polymer for the specific retention of neopterin was developed. A set of 6 polymers was prepared under different experimental condition. The performance of each MIP was tested through their binding capacity. The MIP P4 prepared in the presence of nickel ions exhibited the highest binding capacity 相似文献
This paper reports the synthesis and testing of a molecularly imprinted polymer membrane for digoxin analysis. Digoxin-specific bulk polymer was obtained by the UV initiated co-polymerisation of methacrylic acid and ethylene glycol dimethacrylate in acetonitrile as porogen. After extracting the template analyte, the ground polymer particles were mixed with plasticizer polyvinyl chloride to form a MIP membrane. A reference polymer membrane was prepared from the same mixture of monomers but with no template. The resultant membrane morphologies were examined by scanning electron microscopy. The imprinted membrane was tested as the recognition element in a digoxin-sensitive fluorescence sensor; sensor response was measured using standard solutions of digoxin at concentrations of up to 4 × 10−3 mg L−1. The detection limit was 3.17 × 10−5 mg L−1. Within- and between-day relative standard deviations RSD (n = 5) were in the range 4.5-5.5% and 5.5-6.5% respectively for 0 and 1 × 10−3 mg L−1 digoxin concentrations. A selectivity study showed that compounds of similar structure to digoxin did not significantly interfere with detection for interferent concentrations at 10, 30 and 100 times higher than the digoxin concentration. This simply manufactured MIP membrane showed good recognition characteristics, a high affinity for digoxin, and provided satisfactory results in analyses of this analyte in human serum. 相似文献
A molecularly imprinted polymer (MIP) for the specific retention of neopterin has been developed. A set of 6 polymers was prepared by radical polymerization under different experimental condition using methacrylic acid as functional monomer and ethylene glycol dimethacrylate as crosslinker, with the aim to understand their influence on the efficiency of the MIP. The performance of each MIP was tested in batch experiments via their binding capacity. The MIP prepared in the presence of nickel ions in dimethylsulfoxide-acetonitrile mixture (P4) exhibited the highest binding capacity for neopterin (260 μmol per gram of polymer). A selectivity study with two other pteridines demonstrated the polymer P4 also to possess the best selectivity.