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1.
Kreuzer MP Quidant R Salvador JP Marco MP Badenes G 《Analytical and bioanalytical chemistry》2008,391(5):1813-1820
This work reports the systematic preparation of biosensors through the use of functionalized glass substrates, noble metal
gold colloid, and measurement by localized surface plasmon resonance (LSPR). Glass substrate was modified through chemical
silanization, and the density of gold colloid was carefully controlled by optimizing the conditions of silanization through
the use of mixed silanes and selective mixing procedures. At this point, samples were exposed to bioreagents and changes in
the shallow dielectric constant around the particles were observed by dark-field spectroscopy. Biological binding of high
affinity systems (biotin/streptavidin and antigen/antibody) was subsequently investigated by optimizing coating layers, receptor
concentration profiling, and finally quantitative determination of the analyte of interest, which in this case was a small
organic molecule—the widely used, synthetic anabolic steroid called stanozolol. For this system, high specificity was achieved
(>97%) through extensive nonspecific binding tests, with a sensitivity measurable to a level below the minimum required performance
level (MRPL) as determined by standard chromatographic methods. Analytical best-fit parameters of Hillslope and regression
coefficient are also commented on for the final LSPR biosensor. The LSPR biosensor showed good reproducibility (<5% RSD) and
allowed for rapid preparation of calibration curves and determination of the analyte (measurement time of each sample ca.
2 min). As an alternative method for quantitative steroidal analysis, this approach significantly simplifies the detection
setup while reducing the cost of analysis. In addition the system maintains comparable sensitivity to standard surface plasmon
resonance methods and offers great potential for miniaturization and development of multiplexed devices.
Figure Schematic of sensor configuration indicating both min and max controls and associatedexample localized resonance curves
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
2.
Biosensors that make use of transport processes across lipid membranes are very rare even though a stimulus, the binding of a single analyte molecule, can enhance the sensor response manifold if the analyte leads to the transport of more than one ion or molecule across the membrane. Prerequisite for a proper function of such membrane based biosensors is the formation of lipid bilayers attached to a support that allow for the insertion of membrane peptides and proteins in a functional manner. In this review, the current state of the art technologies to obtain lipid membranes on various supports are described. Solid supported membranes on transparent and electrically conducting surfaces, lipid bilayers on micromachined apertures and on porous materials are discussed. The focus lies on the applicability of such membranes for the investigation of transport phenomena across lipid bilayers facilitated by membrane embedded peptides, channel proteins and transporters. Carriers and channel forming peptides, which are easy to handle and rather robust, are used frequently to build up membrane based biosensors. However, channel forming proteins and transporters are more difficult to insert functionally and thus, there are yet only few examples that demonstrate the applicability of such systems as biosensor devices.
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3.
Dmitrii A. Guschin Halyna Shkil Wolfgang Schuhmann 《Analytical and bioanalytical chemistry》2009,395(6):1693-1706
Electrodeposition polymers can be precipitated on electrode surfaces upon electrochemical-induced modulations of the pH value
in the diffusion zone in front of the electrode. The formed polymer films can be used as immobilization matrices in amperometric
biosensors. In order to rationally control the thus obtained biosensor properties, it is indispensable to develop strategies
for the reproducible synthesis of electrodeposition polymers as well as methods for the non-manual and reproducible sensor
fabrication. Based on instrumental developments such as a specifically designed parallel synthesizer with improved stirring
and temperature control, an automatic pipetting robot for the preparation of the monomer mixtures and controlled removal of
polymerization inhibitors, the reproducible synthesis of libraries of electrodeposition polymers was achieved. Moreover, the
polymerization process could be monitored using in-line thermocouples, and it could be shown that the chosen strategies led
to reproducible polymerization reactions. By adaptation of an electrochemical robotic system integrating a Au microtiter plate
and automatic electrode cleaning by means of a polishing wheel reproducible biosensor fabrication using glucose oxidase as
a model enzyme could be demonstrated. These results open the route for the rational development of biosensors and control
of the sensor properties by choosing specifically designed electrodeposition polymers.
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4.
Urisu T Asano T Zhang Z Uno H Tero R Junkyu H Hiroko I Arima Y Iwata H Shibasaki K Tominaga M 《Analytical and bioanalytical chemistry》2008,391(8):2703-2709
A new planar-type ion channel biosensor with the function of cell culture has been fabricated using silicon on an insulator
substrate as the sensor chip material. Coating of the sensor chip with fibronectin was essentially important for cell incubation
on the chip. Although the seal resistance was quite low (∼7 MΩ) compared with the pipette patch-clamp gigaohm seal, the whole-cell
channel current of the transient receptor potential vanilloid type 1 (TRPV1) channel expressing HEK293 cells was successfully
observed, with a good signal-to-noise ratio, using capsaicin as a ligand molecule.
Figure A new planer type ion channel biosensor with function of cell culture is fabricated using the silicon on insulator substrate
as the sensor chip material. The coating of the sensor chip by the fibronectin was essentially important for the cell incubation
on the chip. Whole cell channel current of TRPV1 channel was successfully observed using capsaicin as a ligand molecule with
good signal to noise. 相似文献
5.
The unique properties of mesoporous silica materials (MPs) have attracted substantial interest for use as enzyme-immobilization
matrices. These features include high surface area, chemical, thermal, and mechanical stability, highly uniform pore distribution
and tunable pore size, high adsorption capacity, and an ordered porous network for free diffusion of substrates and reaction
products. Research demonstrated that enzymes encapsulated or entrapped in MPs retain their biocatalytic activity and are more
stable than enzymes in solution. This review discusses recent advances in the study and use of mesoporous silica for enzyme
immobilization and application in biosensor technology. Different types of MPs, their morphological and structural characteristics,
and strategies used for their functionalization with enzymes are discussed. Finally, prospective and potential benefits of
these materials for bioanalytical applications and biosensor technology are also presented.
Figure Enzyme-functionalized mesoporous silica fibers and their integration in a biosensor design. The immobilization process takes
place essentially in the silica micropores. 相似文献
6.
The use of polymers in microchip fabrication affords new opportunities for the development of powerful, miniaturized separation
techniques. One method in particular, the use of phase-changing sacrificial layers, allows for simplified designs and many
additional features to the now standard fabrication of microchips. With the possibility of adding a third dimension to the
design of separation devices, various means of enhancing analysis now become possible. The application of phase-changing sacrificial
layers in microchip analysis systems is discussed, both in terms of current uses and future possibilities.
Figure Phase-changing sacrificial materials enable multilayer microfluidic device layouts 相似文献
7.
Guo C Song Y Wei H Li P Wang L Sun L Sun Y Li Z 《Analytical and bioanalytical chemistry》2007,389(2):527-532
A novel electrochemical H2O2 biosensor was constructed by embedding horseradish peroxide (HRP) in a 1-butyl-3-methylimidazolium tetrafluoroborate doped
DNA network casting on a gold electrode. The HRP entrapped in the composite system displayed good electrocatalytic response
to the reduction of H2O2. The composite system could provide both a biocompatible microenvironment for enzymes to keep their good bioactivity and
an effective pathway of electron transfer between the redox center of enzymes, H2O2 and the electrode surface. Voltammetric and time-based amperometric techniques were applied to characterize the properties
of the biosensor. The effects of pH and potential on the amperometric response to H2O2 were studied. The biosensor can achieve 95% of the steady-state current within 2 s response to H2O2. The detection limit of the biosensor was 3.5 μM, and linear range was from 0.01 to 7.4 mM. Moreover, the biosensor exhibited
good sensitivity and stability. The film can also be readily used as an immobilization matrix to entrap other enzymes to prepare
other similar biosensors.
Figure Horseradish peroxidase (HRP) embedded in a 1-butyl-3-methylimidazolium tetrafluoroborate (BMIM·BF
4
) doped DNA network can be used to fabricate a HRP sensor for the determination of H2O2 相似文献
8.
The use of electrochemical impedance spectroscopy for biosensing 总被引:1,自引:0,他引:1
This review introduces the basic concepts and terms associated with impedance and techniques of measuring impedance. The focus
of this review is on the application of this transduction method for sensing purposes. Examples of its use in combination
with enzymes, antibodies, DNA and with cells will be described. Important fields of application include immune and nucleic
acid analysis. Special attention is devoted to the various electrode design and amplification schemes developed for sensitivity
enhancement. Electrolyte insulator semiconductor (EIS) structures will be treated separately.
Figure An alternating current which is forced to pass an interface is sensitive to surface changes and will detect impedance changes
due to biomolecule immobilisation or formation of a recognition complex. This can be used for the construction of biosensor
electrodes 相似文献
9.
Alain Walcarius 《Analytical and bioanalytical chemistry》2010,396(1):261-272
Nano- and/or macrostructuring of electrode surfaces has recently emerged as a powerful method of improving the performances
of electrochemical devices by enhancing both molecular accessibility and rapid mass transport via diffusion, by increasing
the electroactive surface area in comparison to the geometric one, and/or by providing confinement platforms for hosting suitable
reagents. This brief overview highlights how template technology offers advantages in terms of designing new types of porous
electrodes—mostly based on thin films, and functionalized or not—and discusses their use in analytical chemistry via some
recent examples from the literature on electrochemical sensors and biosensors.
相似文献
10.
Nicolas F. Y. Durand Elli Saveriades Philippe Renaud 《Analytical and bioanalytical chemistry》2009,394(2):421-425
In this work, we present theoretical and experimental studies of nanofluidic channels as a potential biosensor for measuring
rapid protein complex formation. Using the specific properties offered by nanofluidics, such as the decrease of effective
diffusion of biomolecules in confined spaces, we are able to monitor the binding affinity of two proteins. We propose a theoretical
model describing the concentration profile of proteins in a nanoslit and show that a complex composed by two bound biomolecules
induces a wider diffusion profile than a single protein when driven through a nanochannel. To validate this model experimentally,
we measured the increase of the fluorescent diffusion profile when specific biotinylated dextran was added to fluorescent
streptavidin. We report here a direct and relatively simple technique to measure the affinity between proteins.
Figure We present theoretical and experimental studies of nanofluidic channels as potential biosensors for rapidly measuring protein
complex formation. Our system is based on steady-state diffusion effects which are observed inside a nanoslit. 相似文献
11.
Ma X Zhu T Xu H Li G Zheng J Liu A Zhang J Du H 《Analytical and bioanalytical chemistry》2008,390(4):1133-1137
A chemical prototype sensor was constructed based on nanofiber-structured TiO2 and highly sensitive quartz resonators. The gas-sensing behavior of this new sensor to selected simulant warfare agents was
investigated at room temperature. Results showed rapid response and good reversibility of this sensor when used with high-purity
nitrogen. This provides a simple approach to preparation of materials needed as chemical sensors for selected organic volatiles
or warfare agents.
Figure Sensing behavior of TiO2 nanofiber sensor to chemical vapors 相似文献
12.
Subash C. B. Gopinath Koichi Awazu Makoto Fujimaki Katsuaki Sugimoto Yoshimichi Ohki Tetsuro Komatsubara Junji Tominaga Penmetcha K. R. Kumar 《Analytical and bioanalytical chemistry》2009,394(2):481-488
Biological self-assembly is a natural process that involves various biomolecules, and finding the missing partner in these
interactions is crucial for a specific biological function. Previously, we showed that evanescent-field-coupled waveguide-mode
sensor in conjunction with a SiO2 waveguide, the surfaces which contain cylindrical nanometric holes produced by atomic bombardment, allowed us to detect efficiently
the biomolecular interactions. In the present studies, we showed that the assembly of biomolecules can be monitored using
the evanescent-field-coupled waveguide-mode biosensor and thus provide a methodology in monitoring assembly process in macromolecular
machines while they are assembling.
Evanescent-field-coupled waveguide-mode sensor
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
13.
Catechol determination in compost bioremediation using a laccase sensor and artificial neural networks 总被引:1,自引:0,他引:1
Tang L Zeng G Liu J Xu X Zhang Y Shen G Li Y Liu C 《Analytical and bioanalytical chemistry》2008,391(2):679-685
An electrochemical biosensor based on the immobilization of laccase on magnetic core-shell (Fe3O4–SiO2) nanoparticles was combined with artificial neural networks (ANNs) for the determination of catechol concentration in compost
bioremediation of municipal solid waste. The immobilization matrix provided a good microenvironment for retaining laccase
bioactivity, and the combination with ANNs offered a good chemometric tool for data analysis in respect to the dynamic, nonlinear,
and uncertain characteristics of the complex composting system. Catechol concentrations in compost samples were determined
by using both the laccase sensor and HPLC for calibration. The detection range varied from 7.5 × 10–7 to 4.4 × 10–4 M, and the amperometric response current reached 95% of the steady-state current within about 70 s. The performance of the
ANN model was compared with the linear regression model in respect to simulation accuracy, adaptability to uncertainty, etc.
All the results showed that the combination of amperometric enzyme sensor and artificial neural networks was a rapid, sensitive,
and robust method in the quantitative study of the composting system.
Figure Structure of the magnetic carbon paste electrode used in the electrochemical biosensor 相似文献
14.
Vasiliki Stavyiannoudaki Vicky Vamvakaki Nikos Chaniotakis 《Analytical and bioanalytical chemistry》2009,395(2):429-435
The properties of native and oxidised graphene layered carbon nanofibres are compared, and their utilisation in enzyme biosensor
systems using different immobilisation methods are evaluated. The efficient oxidation of carbon nanofibres with concentrated
H2SO4/HNO3 is confirmed by Raman spectroscopy while the introduction of carboxylic acid groups on the surface of the fibres by titration
studies. The oxidised fibres show enhanced oxidation efficiency to hydrogen peroxide, while at the same time they exhibit
a more efficient and selective interaction with enzymes. The analytical characteristics of biosensor systems based on the
adsorption or covalent immobilisation of the enzyme glucose oxidase on carbon nanofibres are compared. The study reveals that
carbon nanofibres are excellent substrates for enzyme immobilisation allowing the development of highly stable biosensor systems.
Figure Immobilization of proteins on carbon nanofibres 相似文献
15.
Kamisetty NK Pack SP Nonogawa M Devarayapalli KC Kodaki T Makino K 《Analytical and bioanalytical chemistry》2006,386(6):1649-1655
Aminosilane-treated molecular layers on glass surfaces are frequently used as functional platforms for biosensor preparation.
All the amino groups present on the surface are not available in reactive forms, because surface amino groups interact with
remaining unreacted surface silanol groups. Such nonspecific interactions might reduce the efficiency of chemical immobilization
of biomolecules such as DNA, enzymes, antibodies, etc., in biosensor fabrication. To improve immobilization efficiency we
have used additional surface silanization with alkylsilane (capping) to convert the remaining silanol groups into Si–O–Si
linkages, thereby liberating the amino groups from nonspecific interaction with the silanol groups. We prepared different
types of capped amine surface and evaluated the effect of capping on immobilization efficiency by investigating the fluorescence
intensity of Cy3-NHS (N-hydroxysuccinimide) dye that reacted with amino groups. The results indicate that most of the capped amine surfaces resulted
in enhanced efficiency of immobilization of Cy3-NHS compared with the untreated control amine surface. We found a trend that
trialkoxysilanes had greater capping effects on immobilization efficiency than monoalkoxysilanes. It was also found that the
aliphatic chain of alkylsilane, which does not participate in the capping of the silanol, had an important function in enhancing
immobilization efficiency. These results would be useful for preparation of an amine-modified surface platform, with enhanced
immobilization efficiency, which is essential for developing many kinds of biosensors on a silica matrix.
Enhancement of amine funtionality by capping with alkylsilane 相似文献
16.
Lee JO So HM Jeon EK Chang H Won K Kim YH 《Analytical and bioanalytical chemistry》2008,390(4):1023-1032
Recent advances in nanotechnology have enabled the development of nanoscale sensors that outperform conventional biosensors.
This review summarizes the nanoscale biosensors that use aptamers as molecular recognition elements. The advantages of aptamers
over antibodies as sensors are highlighted. These advantages are especially apparent with electrical sensors such as electrochemical
sensors or those using field-effect transistors.
Figure Feeling proteins with aptamer-functionalized carbon nanotubes 相似文献
17.
Christine Mousty 《Analytical and bioanalytical chemistry》2010,396(1):315-325
Two-dimensional layered inorganic solids, such as cationic clays and layered double hydroxides (LDHs), also defined as anionic
clays, have open structures which are favourable for interactions with enzymes and which intercalate redox mediators. This
review aims to show the interest in clays and LDHs as suitable host matrices likely to immobilize enzymes onto electrode surfaces
for biosensing applications. It is meant to provide an overview of the various types of electrochemical biosensors that have
been developed with these 2D layered materials, along with significant advances over the last several years. The different
biosensor configurations and their specific transduction procedures are discussed.
相似文献
18.
Mercury or silver was electrodeposited on an Au surface to form an Hg–Au or Ag–Au film. Wavelength-modulation SPR biosensors
based on this Hg/Ag–Au film were then used to determine human IgG and rabbit IgG. When direct immunoreactions were performed
on the Au sensing surface, the range of concentrations of human IgG and rabbit IgG that could be determined were 2.00–40.00 μg/ml
and 2.50–40.00 μg/ml, respectively. When Hg was electrodeposited onto the Au film for 1200 s, the range of concentrations
of human IgG and rabbit IgG that could be determined were 0.50–40.00 μg/ml and 0.63–40.00 μg/ml, respectively. When Ag was
electrodeposited onto the Au film for 1500 s, the range of concentrations of human IgG and rabbit IgG that could be determined
were 0.25–20.00 and 0.42–20.00 μg/ml, respectively. The biosensor based on Ag–Au film was therefore found to be the most sensitive
of the three types of biosensor tested, giving limits of determination that were up to eight times lower than those obtained
with a biosensor based on Au film alone.
Figure The relationship between the concentration of human IgG and the shift in the resonant wavelength Δλ
eq for different Ag electrodeposition times 相似文献
19.
Lieberzeit P Greibl W Jenik M Dickert FL Fischerauer G Bulst WE 《Analytical and bioanalytical chemistry》2007,387(2):561-566
Silanisation of quartz substrate surfaces with a mixture of two chlorosilanes, namely trimethylchlorosilane and 7-octenyldimethylchlorosilane,
leads to sensitive coatings for volatile organic compounds (VOC) on surface acoustic wave (SAW) devices. In this way we created
monolayers of molecular cavities engulfing the analytes according to host–guest chemistry directly on the device surfaces,
and also confirmed the occurrence of such cavities by molecular modelling. We monitored the binding process of the silanes
by using Fourier transform infrared (FTIR) spectrometry and atomic force microscopy (AFM). In order to increase the stiffness
of the cavities, we crosslinked the terminal double bonds of the long spacers by heating the surface in the presence of a
radical initiator. Compared to SAW delay lines silanised with trimethylchlorosilane, devices modified with the binary silane
mixture lead to substantially higher frequency shifts when exposed to solvent vapour streams. Nearly instantaneous responses
can be observed, which e.g. allows xylene detection down to a few ppm.
Interaction of o-xylene with a silanised quartz surface
Dedicated to Prof. Adolf Neckel on the occasion of his 80th birthday. 相似文献
20.
Spricigo R Dronov R Lisdat F Leimkühler S Scheller FW Wollenberger U 《Analytical and bioanalytical chemistry》2009,394(1):225-233
An efficient electrocatalytic biosensor for sulfite detection was developed by co-immobilizing sulfite oxidase and cytochrome
c with polyaniline sulfonic acid in a layer-by-layer assembly. QCM, UV–Vis spectroscopy and cyclic voltammetry revealed increasing
loading of electrochemically active protein with the formation of multilayers. The sensor operates reagentless at low working
potential. A catalytic oxidation current was detected in the presence of sulfite at the modified gold electrode, polarized
at +0.1 V (vs. Ag/AgCl 1 M KCl). The stability of the biosensor performance was characterized and optimized. A 17-bilayer
electrode has a linear range between 1 and 60 μM sulfite with a sensitivity of 2.19 mA M−1 sulfite and a response time of 2 min. The electrode retained a stable response for 3 days with a serial reproducibility of
3.8% and lost 20% of sensitivity after 5 days of operation. It is possible to store the sensor in a dry state for more than
2 months. The multilayer electrode was used for determination of sulfite in unspiked and spiked samples of red and white wine.
The recovery and the specificity of the signals were evaluated for each sample.
Figure Schematic of the bioelectrocatalytic sulfite sensor: sulfite oxidase (green) oxidizes sulfite to sulfate and transfers electrons via heme b
5 to cyt c (red) and thence to the gold electrode 相似文献