Eremophilane Sesquiterpenes from Cacalia Roborowskii

Themajorityofphytochemicalstudiesonsenencioneace(compositae)specieshavebeenconcentratedonthepyrrolizidinealkaloidsandsesquiteIPenesbecauseoftheirpotentialbioactivitiesandCytotoalcactivities.lCacaliaroborowskii(maxim)LinginslongbeenusedasfolkremedyinnorthwestemChina.Recently,wehadisolatedseveralneweremophilenolides'fromtherootsofthisplant.Asacontinuahonofourinvestigationontillsspecies,wenowpresenttileisolationandsmicturalelucid.ltionoffouraddihonalneweremophilanesesquiterpenes.Theirsmicturesw…     

Two New Eremophilenolides from Cacalia pilgeriana

Two new eremophilenolides, 1β-hydroxy-2β-methylsenecioyloxyeremophil-7 （11）-en- 8β （12）-olide （1）, 1β-hydroxy-2β-methylsenecioyloxy-8α-methoxyeremophil-7 （11）-en-8β （12）- olide （2）, were isolated from the roots of Cacalia pilgeriana. Their structures were elucidated by spectroscopic methods and X-ray diffraction analysis.     

Two New Eremophilenolides from Cacalia ainsliaeflora

Two new eremophilenolides(3β-angeloyloxy-8β,10β-dihydroxy-6β-ethoxyeremophile-nolide(1)and 3β,6β-diangeloyloxy-8α-methoxy-10α-hydroxyeremophilenolide(2)were isolated from the roots of Cacalia ainsliaeflora.Their structures were elucidated by spectroscopic methods.     

Two New ent-Kaurenoids from Cacalia pilgeriana

Two new ent-kaurenoids, 19-acetyl-ent-3β, 17-dihydroxykaur-15-ene (1), 19-acetyl-ent-3β-hydroxykaur-15-en-17-al (2) were isolated from Cacalia pilgeriana. Their structures were elucidated by spectroscopic methods.     

New Sesquiterpenes from Cacalia ainsliaeflora

Two new eremophilane sesquiterpenes, 3β-angeloyloxy-8-oxo-eremophil-6(7)-en-12-oic acid 1 and 3β-angeloyloxy-10β-hydroxy-8-oxo-eremophil-6(7)-en-12-oic acid 2, and a novelnor-eremophilane derivative, 3β-angeloyloxy-10β-hydroxy-8-oxo-eremophil-6(7)-en 3 were isolated from the roots of Cacalia ainsliaeflora. Their structures were elucidated by spectroscopic methods, including 2D NMR.     

Two New Eremophilane Sesquiterpenes from Cacalia ainsliaeflora

The root of C. ainsliaeflora has been used for curing pellagra, rheumatismal edema and insecticide1. In this paper, we report the structure elucidation of two new eremophilane sesquiterpenes (1, 2) from the roots of C. ainsliaeflora. Compound 1, colorless gum, [(]: -50 (c 0.50, CHCl3). HRESI-MS showed [M+H]+ at m/z 377.19528 (calcd for C21H29O6 377.19587), corresponding to a molecular formula C21H28O6. Its IR bands (1655, 1717, 1750cm-1) and UV absorptions (221 nm) displayed the typi…     

Terpenoids from the Flower of Cacalia Tangutica

A thorough investigation of Cacalia tangutica (Franch.) Hand‐Mazz afforded two new epimeric eremophilane sesquiterpenoids, 7β‐ H‐3α‐angeloyloxy‐9‐ene‐11,12‐epoxy‐8‐oxoeremophilane ( 1 ) and 7α‐H‐3α‐angeloyloxy‐9‐ene‐11,12‐epoxy‐8‐oxoeremophilane ( 2 ), as well as four known sesquiterpenes, petasol ( 3 ), oplopanone ( 4 ), 4,5‐epoxy‐β‐caryophyllene ( 5 ), 4(15)‐eudesmene‐1β,6α‐diol ( 6 ), and three known monoterpenes, 1β,2α‐dihydroxy‐p‐menth‐5‐ene ( 7 ), 5α‐hydroxy‐2‐oxo‐p‐menth‐6(1)‐ene ( 8 ), 5β‐hydroxy‐2‐oxo‐p‐menth‐6(1)‐ene ( 9 ), and two known triterpenes, maniladiol 3‐O‐palmitate ( 10 ), taraxasteryl palmitate ( 11 ). The new compounds were characterized by means of spectroscopic methods including 1D, 2D NMR and HRESIMS, and the known ones were established on the basis of comparing their NMR data with those of the corresponding compounds in the literature. In addition, cytotoxicity against selected cancer cells (HL‐60, HO‐8910 and A‐549) of compounds 1 ‐ 4 , 6 , 7 , 10 were measured in vitro.     

A New Sesquiterpene and a New Norsesquiterpene from Cacalia deltophylla

A new sesquiterpene and a new norsesquiterpene were isolated from the whole plant of Cacalia deltophylla (Maxim) Mattf. Their structures were elucidated as deltocacalone (1) and deltonorcacalol (2) by spectroscopic methods including 2D NMR.     

Two Novel Epimeric Eremophilane Sesquiterpenes from the Flower of Cacalia tangutica

Two novel epimeric eremophilane sesquiterpenes, 7β-H-3α-angeloyl-9(10)-ene-11,12-epoxy-8-oxoeremophilane (1) and 7β-H-3α-angeloyl-9(10)-ene-11, 12-epoxy-8-oxoeremophilane (2) were isolated from the methanol extract of the flower of Cacalia tangutica (Franch.) Hand-Mazz. Their structures were characterized by 1D-, 2D-NMR (^1H-^1H COSY, HMQC, HMBC, ^1H-^1H NOESY) and HRESI-MS techniques.     

Separation and determination of coumarins from Cacalia tangutica by capillary zone electrophoresis

Capillary zone electrophoresis (CZE), using a 20 mmol/L borate buffer at pH 10.5, was developed for the identification and determination of three coumarins--7-hydroxy-coumarin (HC), 7-hydroxy-8-methoxy-coumarin (HMC) and 7-O-beta-D-glucosyl-coumarin (GC)--in the extracts of the flower of Cacalia tangutica. Regression equations revealed linear relationships (correlation coefficient 0.9986-0.9990) between the corrected peak area (the ratio of peak area to migration time) of each constituent and its concentration. The relative standard deviations (RSD) of the migration time and peak area were 1.45-1.52 and 2.60-3.84% (intra-day), and 1.75-2.22 and 2.90-4.04% (inter-day), respectively. The recoveries of three constituents ranged between 94.5 and 105.6%. The effects of pH value, buffer concentration and applied voltage on the migration behavior of HC, HMC and GC were investigated. The contents of the three active constituents in the flower of Cacalia tangutica were successfully determined within 6 min under the optimum conditions chosen. Moreover, the dissociation constants for three coumarins were also determined by CE.     

New Eremophilenolides from Senecio dianthus

From the aerial parts of Senecio dianthus, four new eremophilenolides ( 1 – 4 , resp.) and one new eremophilenolide alkaloid ( 5 ), of the relatively uncommon eremophilenoid‐type sesquiterpenoid lactones, were isolated together with three known sesquiterpenoid lactones, 10β‐hydroxyeremophil‐7(11)‐en‐12,8α‐olide ( 6 ), 8β,10β‐dihydroxyeremophil‐7(11)‐en‐12,8α‐olide ( 7 ), and 10α‐hydroxy‐1‐oxoeremophila‐7(11),8(9)‐dien‐12,8‐olide ( 8 ). On the basis of IR, MS, and NMR data, particularly 2D‐NMR analyses, the structures of the new compounds were established as: 2β‐(angeloyloxy)‐10β‐hydroxyeremophil‐7(11)‐en‐12,8α‐olide ( 1 ), 6β‐(angeloyloxy)‐10β‐hydroxyeremophil‐7(11)‐en‐12,8α‐olide ( 2 ), 2β‐(angeloyloxy)‐8β,10β‐dihydroxyeremophil‐7(11)‐en‐12,8α‐olide ( 3 ), 2β‐(angeloyloxy)‐8α‐hydroxyeremophila‐7(11),9(10)‐dien‐12,8β‐olide ( 4 ), and 8β‐amino‐10β‐hydroxyeremophil‐7(11)‐en‐12,8α‐olide ( 5 ). In addition, the relative configuration of 1 was corroborated by X‐ray diffraction analysis.     

Biologically Active Substances from Cacalia hastate Leaves. 1. Carbohydrates from Leaves and Their Hypoglycemic Activity

Leaves of Cacalia hastate L. (Asteraceae) were composed of free sugars, water-soluble polysaccharides (arabinogalactan type), pectinic substances, hemicelluloses, and cellulose. The hypoglycemic activity was determined for the water-soluble polysaccharides and pectinic substances.     

Two New Eremophilenolides from Ligularia muliensis

Many Ligularia species have long been used as traditional folk medicine for theirantibiotic, antiphlogistic and antitussive activities1, and were found to be an importantsource of eremophilane. In the course of our search for bioactive sesquiterpenoids, w…     

Two New Eremophilenolides from Ligularia fischeri

Ligulariajischerihasl0ngbeenusedastraditi0naImedicinet0relievec0ugh,invig0ratethecircuIation0fbIo0dandst0ppain'.FromtheplantgrowinginShengnonaiia,Hubei-Chlna,t\v0neweremophiIen0lideshavebeenisolated.Comp0undlwasneedlecrystalsfrompetr0lether(60-9O'C),m.p.=148-l50'C.ItsformulawasdeterminedasC,,H,'O'by"C-NMRandDEPTspectrainaccordancewiththem0lecularionpeakm/z=280inEIMS.Thetypeofcarbonsignals(5xC,3xCH,4xCH,,4xCH,)(Table2)sh0wedithadabicyclicsesquiterpeneskeletonbearingameth0xy.ahemi-…     

Two New Eremophilenolides from Ligularia sagitta

Chemical investigation of L.sagitta afforded two new eremophilenolides, which were identified as 6β-angeloyloxy-10β-hydroxy-8 β-methoxy-eremophil-7(11)-en-12, 8α-olide (1) and 6β, 8β-dimethoxy-10β-hydroxy-eremophil-7(11)-en-12, 8α-olide (2). Their structures were estab- lished by spectroscopic methods including 2D NMR experiments.     

青海省唐古特铁线莲中齐墩果酸含量的测定

建立了唐古特铁线莲中齐墩果酸含量测定的HPLC方法。色谱条件:采用Phenomenex Luna C18(250 mm×4.6 mm,5μm),流动相:乙腈-0.2%H3PO4(35:65),流速:1 mL/min,柱温:室温(25℃),检测波长:205 nm。齐墩果酸在0.0233～0.7000 mg范围内呈良好的线性关系,线性回归方程为y=2E+06x+360803,R2=0.9917,回收率RSD为1.7%,并对青海省野生和栽培唐古特铁线莲中齐墩果酸进行了定量分析,结果显示前者平均值为0.1184%,后者平均值为0.0651%。方法可用以测定唐古特铁线莲中齐墩果酸的含量。     

Diterpenoids from Rabdosia Nervosa

Six ent-kaurene type diterpenoids were isolated from the leaves of Rabdosia nerwsa. Among them.ganer-vosin A and B are new compounds, the structures of which were elucidated as 1 and 2 respectively by spectral and chemical methods. The other four were identified as odonicin (3), novelrabdosin (4), nodosin (5) and shikokianal acetate (8). Compounds (1), (2), (5) and (8) have not been found in this herb previously.     

核桃楸叶挥发油化学成分分析

采用水蒸气蒸馏法提取核桃楸叶中的挥发油,从核桃楸叶挥发油中分离了57种化合物,用气相色谱/质谱联用法鉴定了51种化合物,占被分离化合物的89.46%,其中烃类(33种,48.03%)、酮类(1种,0.55%)、醇类(5种,25.94%)、酯类(6种,8.43%)、酸类(1种,0.62%)、含氧杂环类(4种,2.62%)及甲酰类(1种,0.65%)等7大类化合物,有3类(27种,51.19%)为已知药用成分,这些数据为新药开发提供了科学信息.     

A novel flavonoid from the root of Macrothelypteris torresiana （Gaud.） Ching

A novel flavonoid was isolated from the root of Macrothelypteris torresiana(Gaud.) Ching.The structure of the product was identified as 5,7-dihydroxy-2-(1,2-isopropyldioxy-4-oxocyclohex-5-enyl)-chromen-4-one on the ground of chemical and spectroscopic methods.     

中药药对的化学成分研究川芎-赤芍挥发油的GC/MS分析

中药药对是复方的最小组成单位,具有中药配伍的基本特点. 药对化学是复方化学的核心内容. 联用色谱和化学计量学方法为中药复杂体系的分离与分辨提供了强有力的工具. 采用GC-MS法分离测定中药药对川芎-赤芍、 单味药川芎和赤芍的挥发油成分,并对其中的重叠色谱峰采用化学计量学解析方法(CRM)进行分辨,得到药对和各单味药的纯色谱曲线和质谱图,药对川芎-赤芍、 单味药川芎和赤芍分别分辨出82,78和57个色谱峰,通过质谱库对分辨的纯组分进行定性,分别得到61,52和33个定性结果,占总含量的90.18%,95.14%和95.82%.