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1.
High-performance liquid chromatographic fingerprint analysis for different origins of sea buckthorn berries 总被引:1,自引:0,他引:1
Using high-performance liquid chromatography (HPLC), a chemical fingerprint method was developed for investigating and demonstrating the variance of flavonoids among different origins of sea buckthorn berries. Thirty-four samples were analyzed including 15 RS (Hippophae rhamnoides ssp. sinensis) samples, 7 RY (H. rhamnoindes ssp. yunnanensis) samples, 5 RW (H. rhamnoides ssp. wolongensis) samples, 4 NS (H. neurocarpa ssp. stellatopilosa) samples and 3 TI (H. tibetana) samples. In the HPLC chromatograms, 12 compounds were identified as flavonoids, including quercetin 3-O-sophoroside-7-rhamnoside, kaempferol 3-O-sophoroside-7-O-rhamnoside, isorhamnetin 3-O-sophoroside-7-O-rhamnoside, isorhamnetin 3-O-glucoside-7-O-rhamnoside, quercetin 3-O-rutinoside, quercetin 3-O-glucoside, isorhamnetin 3-O-rutinoside, isorhamnetin 3-O-glucoside, quercetin, kaempferol 7-O-rhamnoside, kaempferol and isorhamnetin. Both correlation coefficient of similarity in chromatograms and relative peak areas of characteristic compounds were calculated for quantitative expression of the HPLC fingerprints. Our results revealed that the chromatographic fingerprint combining similarity evaluation could efficiently identify and distinguish sea buckthorn berries from different species. However, no obvious difference between RS and RY suggested that the two subspecies might have very close relationship in terms of chemotaxonomy. The established method was considered to be suitable for fingerprint analysis to check the genuine origin and control the quality of sea buckthorn berries and extracts. 相似文献
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原料奶的高效液相色谱指纹图谱的建立 总被引:1,自引:0,他引:1
采用高效液相色谱-质谱联用(HPLC-MS)技术,在负离子扫描模式下建立了原料奶的乙腈提取成分的指纹图谱。采用乙腈和0.1%的乙酸水溶液为流动相进行二元梯度洗脱,柱温30℃,分析时间为85min。确定了11个共有峰,以其中的7号峰为参照物,通过相对峰面积和相对保留时间进行了方法学考察。结果表明,本方法具有良好的重现性,各指纹峰相对保留时间的相对标准偏差(RSD)小于0.79%,相对峰面积的RSD小于2.84%。在原料奶指纹图谱基础上,选取有代表性的危害物如防腐剂苯甲酸等进行了添加,建立了危害物添加图谱,对沈阳地区超市的50个纯牛奶样品进行了筛查,取得初步的应用结果。 相似文献
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Development of high-performance liquid chromatographic fingerprints for distinguishing Chinese Angelica from related umbelliferae herbs 总被引:6,自引:0,他引:6
Lu GH Chan K Liang YZ Leung K Chan CL Jiang ZH Zhao ZZ 《Journal of chromatography. A》2005,1073(1-2):383-392
A high-performance liquid chromatographic (HPLC) fingerprint of Chinese Angelica (CA) was developed basing on the consistent chromatograms of 40 CA samples (Angelica sinensis (Oliv.) Diels). The unique properties of this HPLC fingerprints were validated by analyzing 13 related herbs including 4 Japanese Angelicae Root samples (JA, A. acutiloba Kitagawa and A. acutiloba Kitagawa var. sugiyame Hikino), 6 Szechwan Lovage Rhizome samples (SL, Ligusticum chuanxiong Hort.) and 3 Cnidium Rhizome samples (CR, Cnidium officinale Makino). Both correlation coefficients of similarity in chromatograms and relative peak areas of characteristic compounds were calculated for quantitative expression of the HPLC fingerprints. The amount of senkyunolide A in CA was less than 30-fold of that in SL and CR samples, which was used as a chemical marker to distinguish them. JA was easily distinguished from CA, SL and CR based on either chromatographic patterns or the amount of coniferyl ferulate. No obvious difference between SL and CR chromatograms except the relative amount of some compounds, suggesting that SL and CR might have very close relationship in terms of chemotaxonomy. Ferulic acid and Z-ligustilide were unequivocally determined whilst senkyunolide I, senkyunolide H, coniferyl ferulate, senkyunolide A, butylphthalide, E-ligustilide, E-butylidenephthalide, Z-butylidenephthalide and levistolide A were tentatively identified in chromatograms based on their atmospheric pressure chemical ionization (APCI) MS data and the comparison of their UV spectra with those published in literatures. 相似文献
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A novel, accurate and valid fingerprint method was developed by using high-performance liquid chromatography-photodiode array detection (HPLC-DAD) to control the quality of Rheum tanguticum Maxim. ex Balf. By comprehensively comparing chromatograms of all samples, our result revealed that the chromatographic fingerprint we developed combining similarity evaluation could efficiently identify and distinguish raw materials of R. tanguticum from different sources. Besides, 21 characteristic peaks in chromatogram of R. tanguticum were unambiguously confirmed by comparison of reference compounds based on their retention time (t(R)) and UV spectra. 相似文献
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Over 150 solute-micelle association (binding) constants and free solute retention factors are reported for a variety of neutral compounds and PTH-amino acids. The solute-micelle parameters were calculated from critically evaluated micellar liquid chromatographic data. The potential utility of these parameters for the optimization of micellar liquid chromatography and micellar electrokinetic capillary chromatography, including selection of the surfactant and/or stationary phase and the optimization of surfactant concentration, is briefly discussed. 相似文献
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Summary A semi-preparative high-performance liquid chromatographic procedure on 5-m silica was developed for the isolation of gram quantities of ethyltrans-5-iodo-trans-2-methylcyclohexane-1-carboxylate (B1) and ethyltrans-4-iodo-trans-2-methylcyclohexane-1-carboxylate (B2) for comparative evaluation as male Mediterranean fruit fly,Ceratitis capitata, attractants and for NMR studies. This procedure can also be used analytically to determine the content of B1 (the attractive isomer) in ceralure, the ethyl 4- and 5-iodo-trans-2-methylcyclohexane-1-carboxylate mixture. 1,1-Dimethylethylcis-5-iodo-trans-2-methylcyclohexane-1-carboxylate (A) and 1,1-dimethylethylcis-4-iodo-trans-2-methylcyclohexane-1-carboxylate (C) were isolated and converted to their ethyl esters, thus supplying the fourtrans-isomers of ceralure. 相似文献
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A simple and accurate quantitative determination of aspartame (L-alpha-aspartyl-L-phenylalanine methyl ester), a new artificial sweetener, is described. The method, which is based on ion-pair high-performance liquid chromatography, allows the determination of aspartame in finished bulk and dosage forms, and the detection of a few related products at levels down to 0.1%. 相似文献
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Summary Nitroxoline (8-hydroxy-5-nitroquinoline) and the structural related compounds 8-hydroxyquine, 8-hydroxy-5,7-dinitroquinoline,
and 8-hydroxy-5-nitrosoquinoline have been investigated by reversed-phase high-performance liquid chromatography on C18/ODS. Complete separation and symmetric, peaks were obtained by use of THF-methanol-water, 3∶3∶4, containing 10 mmol L−1 disodium ethylenediamine tetraacetic acid (EDTA) and 10 mmol L−1 citric acid in the water, as mobile phase. The pH and the concentration of EDTA in the mobile phase were found to be critical
for eliminating tailing and for full separation. The calibration plot was linear for concentrations between 3.0 and 300 μg
mL−1; the regression coefficient was 0.99996. Assay of the nitroxoline standard showed that recovery was from 99.3 to 102%, with
a mean standard deviation for nitroxoline of 0.9%. The method is suitable for quality control of nitroxoline. 相似文献
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Molecular species of neutral phospholipids, phosphatidylcholine (PC) and phosphatidylethanolamine (PE), were resolved by reversed-phase high-performance liquid chromatography (HPLC) using mobile phases of acetonitrile-methanol-water containing tetraalkylammonium phosphates (TAAPs). Competitive interactions of TAAPs and analyte solutes with a reversed-phase HPLC column resulted in reduced retention of PC or PE with concomitant increase in detection sensitivity. The chromatographic data for PC and PE were distinctly different from those for negatively charged phospholipids where ion-pair retention mechanisms prevailed. While PC (or PE) components eluted at longer retention times with a larger size of TAAP, an increase in the TAAP concentration invariably caused a decrease in phospholipid retention times. Optimization of HPLC conditions by using high concentrations (25-100 mM) of tetramethylammonium phosphate in acetonitrile-methanol-water (70:22:8) facilitated elution of components with improved peak symmetry. HPLC separations of neutral phospholipids derived from animal sources were more complex than those from soybeans. 相似文献
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长期以来,中药有效成分的提取分离和质量控制一直是中药产业中特有和尚未很好解决的问题.随着天然产物化学和现代仪器分析技术等相关学科的迅速发展,国内外在植物药及其制剂质量控制研究方面的重点均已转向利用各种仪器进行品质评价方面.中药来源于自然,其化学成分的多样性和复杂性是中药发挥其疗效的物质基础,同时也是质量评价与控制的重点与难点.中药的化学成分复杂,所体现的是综合效应和整体疗效.然而多年以来在中药的研究靠中,采取了与化学药品相同的研究思路,结果将药材及制剂内部各成分的综合作用和相互关系彼此孤立.在中药材及复方制剂质量标准的研究制定中,也往往是采用各种分析检测手段测定其中某种有效成分,并以其含量多少来判断比较某种药材的质量.这种质量控制模式很难做到全面衡量中药及其制剂的质量、疗效和稳定性.单一成分含量达标并不能说明该中药材质量合格.指纹图谱质量控制模式能综合反映某一特定药材各主要组分及其相对含量.因此它比测定任何一种或几种成分所提供的信息都丰富和有用的多.中药提取物在给定的实验条件下所得到色谱指纹图谱反映了该中药的化学组成及其含量分布状况,其特征峰可作为鉴别中药的依据.本文研究结果表明,利用色谱指纹图谱的相关参数来对中药原药材的质量进行控制,进而使中成药的质量得到保证,此方法的运用是切实可行的.另外,在有效成分不明确的前提下,利用色谱指纹图谱来评价中药的质量是有其意义的.色谱指纹图谱作为控制中药质量的新手段将在推进中药现代化进程上起到积极的作用.本文利用高效液相色谱建立了地黄的色谱指纹图谱.采用反相C18柱(5μm,150 mm×4.6 mm)、流动相甲醇:水(V/V=5:95)、检测波长为280 nm、流速1.0 mL/min进行试验.根据相对保留值和相对面积值对色谱指纹图谱进行分析对比研究,建立了控制地黄药材质量的新方法.该法为中药样品的鉴定提供了较全面的信息. 相似文献
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The influences of fermentation conditions and truffle species (i.e., Tuber melanosporum, Tuber sinense, Tuber indicum, and Tuber aestivum) on the volatile organic compounds (VOCs) originated from truffle fermentation mycelia were studied by using chromatographic fingerprint system for the first time. Gas chromatography combined with statistical methods including similarity analysis and hierarchical cluster analysis was applied to develop chromatographic fingerprint system for truffle VOCs evaluation. Fermentation conditions affected the VOCs from truffle fermentation mycelia much more significantly than truffle species. This indicated that it is possible to adjust the aroma of truffle fermentation mycelia similar with the natural fruiting-body through the control of fermentation process. 相似文献
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High-performance liquid chromatographic analysis for the characterization of triterpenoids from Ganoderma 总被引:1,自引:0,他引:1
A high-performance liquid chromatographic (HPLC) analysis of triterpenoids from Ganoderma is developed and validated in an attempt to explore a way to differentiate a number of species of the genus Ganoderma. Results show that 64 samples examined in this study could be divided into 18 groups based on characteristics of the HPLC pattern of triterpenoids. This result also conforms with those of the morphological examination and the interfertility test by di-monokaryotic mating. The HPLC analysis of triterpenoids further reveals that differentiation among samples from three different regions each of the two species G. lucidum and G. tsugae is workable. Even then, an incorrect designation is found for two of the groups of samples that were originally classified as G. resinaceum but showed different morphological characteristics and mating incompatibility. In conclusion, an HPLC analysis of triterpenoids is a simple and easy way to differentiate among different species of the genus Ganoderma. 相似文献
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Annalisa Pirini Lanfranco S. Conte Ornella Francioso Giovanni Lercker 《Journal of separation science》1992,15(3):165-170
The chemical analysis of honey tends to concentrate on factors related to its state of preservation, e.g. HMF, diastase activity, and water content. Although there is no characteristic of honey officially regarded as suitable for certification of its botanical origin, literature is available in which several of the “minor” components of honey, such as flavors, di- and trisaccharides, and free amino acids, have been used to certify the botanical origin of the product. In this paper, six kinds of honey from different botanical sources (acacia, citrus fruit, chestnut-tree, rhododendron, rosemary, and lime-tree) were analyzed by capillary gas chromatography, and the data obtained evaluated statistically to determine whether the amino acid profile could be used to verify the botanical source of the material. The results have shown that the presence of amino acids such as arginine, tryptophan, and cystine is characteristic of a particular kind of honey, and that others, such as proline, asparagine, lysine, and methionine, can be used for discrimination if quantitative data is available about the levels of the compounds present. Evaluation of optimum split ratio for amino acid determination, and problems concerning the derivatization process, are also discussed. 相似文献