Green synthesis of antibacterial chitosan films loaded with silver nanoparticles

An eco-friendly chemical reduction method was successfully used for the preparation of chitosan (CTS) composite films loaded with silver nanoparticles (AgNPs) by self assembly method using poly(ethylene glycol) as both reducing and stabilizing agent. UV-Vis spectra of the prepared chitosan loaded silver nanoparticles (CTSLAg) films reveal that full reduction of silver ions to silver nanoparticles takes place at 90 °C. The effect of reaction conditions on the silver nanoparticles formation was investigated using UV-Vis spectrophotometer. The morphology of the films was tested by scanning electron microscopy (SEM). The DSC curves showed that the CTSLAg film had a favorable compatibility and heat stability. AgNPs were confirmed by XRD and UV-Vis spectroscopy. The TEM findings revealed that the silver nanoparticles synthesized were spherical in shape with uniform dispersal, and by increasing CTS:PEG ratio larger silver nanoparticles could be obtained. The results of antibacterial study reveal that the prepared nanocomposite films exhibited potential inhibition.     

Synthesis of poly(p-dioxanone)-based block copolymers in supercritical carbon dioxide

Poly(p-dioxanone)–poly(ethylene glycol)–poly(p-dioxanone) triblock copolymers (PPDO–PEG–PPDO) were first synthesized by suspension ring-opening polymerization (ROP) of p-dioxanone (PDO) in supercritical carbon dioxide (scCO₂) using different molecular weights (2–10 K) of poly(ethylene glycol) (PEG) as macroinitiators. White and fine flow powders were successfully obtained when the molecular weight of PEG was below 6 K and its feed content below 20 wt.%. The ¹H nuclear magnetic resonance (NMR) result indicated the formation of PPDO–PEG–PPDO block structure even in a confined polymerized environment of particles. All the powderous samples contained irregular shaped particles that were observed by scanning electron microscope (SEM). Except for the copolymer with 10 wt.% PEG10K feed content, the mean particle sizes of other powderous samples showed identical values close to 15 μm. This fact was in agreement with the crystallinity of PPDO in the copolymers measured by differential scanning calorimetry (DSC). The water absorption of these copolymers was also measured, and as compared with PPDO homopolymer, the introduction of PEG increased the water absorption of the copolymers. The green and environmentally friendly method disclosed in this work is attractive to directly synthesize biodegradable polymeric particles with potential biomedical applications.     

Green synthesis of chitosan-stabilized silver-colloidal nanoparticles immobilized on white-silica-gel beads and the antibacterial activities in a simulated-air-filter

This study explored the green synthesis and immobilization of colloidal silver nanoparticles (AgNPs) on a solid compatible support. Its antibacterial properties in reusable air filters are also discussed. The chitosan stabilized colloidal AgNPs (chi-AgNPs) were prepared using visible light irradiation in methanol. The UV–Vis, FTIR spectra, and TEM confirmed the chi-AgNPs formation. The immobilization technique of chi-AgNPs on the surface of white-silica-gel beads, which was previously coated chitosan (chi-SiG), was effective. The immobilized silver particles (AgNPs-[chi-SiG]) were solid, stable, dispersed, and nano-size. Both AgNPs-[chi-SiG] and chi-SiG exhibited antibacterial properties and prevented the growth of Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria in agar media. Air filter containing the AgNPs-[chi-SiG] showed high antibacterial activity against Bacillus subtilis in the air.     

Comparative study of the sol–gel based solid phase microextraction fibers in extraction of naphthalene, fluorene, anthracene and phenanthrene from saffron samples extractants

We are introducing a method for the determination of some polycyclic aromatic hydrocarbons in aqueous saffron sample by direct immersion solid phase microextraction (SPME) and gas chromatography. A sol–gel technique is used for the preparation of the SPME fibers. Three kinds of sol–gel coatings on the fibers were tested and compared. They are composed of poly(dimethyl siloxane) (PDMS), poly(ethylene glycol) (PEG), and a poly(ethylene glycol) modified with multi-walled carbon nanotubes (PEG/CNTs). The effects of fiber coating, desorption time, desorption temperature, extraction time, stirring speed and salting effect were optimized. Under the optimal conditions, the detection limits (at S/N?=?3) are 7–50, 5–50, and 1–10?pg?mL^–1, respectively, for SPME fibers made from PDMS, PEG and PEG/CNTs. The relative standard deviations for one type of fiber are from 2.1% to 9.6% for all fibers (at n?=?5), and in the range from 1.9% to 9.8% from batch to batch (for n?=?3).

Application of Sol–Gel Based Poly(ethylene glycol)/Multiwalled Carbon Nanotubes Coated Fiber for SPME of Methyl tert-Butyl Ether in Environmental Water Samples

In this research, the sol–gel technology was applied for the preparation of solid-phase microextraction fibers for extracting of methyl tert-butyl ether (MTBE) from environmental water samples. For this purpose, two different polymers such as poly(ethylene glycol) (PEG) and combination of PEG and multiwall carbon nanotubes (MWCNTs) were prepared using sol–gel technology as coating procedure for the fibers. The pre-concentration process followed by GC–FID determination was used and the results evidenced that pre-concentration factor for PEG/CNTs fiber was approximately five times higher than PEG. Parameters affecting the extraction efficiency such as temperature, extraction time, stirring speed and salt effect for each fiber were investigated and optimized. On the optimal conditions, the linear range for MTBE with PEG and PEG/CNT fibers were 10–3,000 and 1–1,000 ng mL^?1 and the detection limits (S/N = 3) were 1.0 and 0.3 ng mL^?1, respectively. The sol–gel PEG/CNTs fiber has good performance and therefore relatively better figures of merit and experimental results such as thermal stability (up to 320 °C), average of life time (over 150 times) and repeatability (RSD < 4) in comparison to conventional PDMS/Carboxen fiber, which was already reported for determination of MTBE.     

Synthesis of silver nanoparticles in chitosan, gelatin and chitosan/gelatin bionanocomposites by a chemical reducing agent and their characterization

In this research, silver nanoparticles (AgNPs) were synthesized in chitosan (Cts), Cts/gelatin and gelatin suspensions using a chemical reducing agent. Cts and gelatin were used as natural stabilizers and solid support, whereas AgNO(3) was used as the silver precursor. Sodium borohydride (NaBH(4)) was used as the reducing agent. The properties of AgNPs in Cts, Cts/gelatin and gelatin bionanocomposites (BNCs) were studied in terms of their surface plasmon resonance, crystalline structure, average diameter size, particle distributions, surface topography and functional groups. All the samples were characterized by UV-visible spectroscopy, powder X-ray diffraction, transmission electron microscopy, atomic force microscopy and Fourier transform infrared spectroscopy.     

Novel crosslinked thermoresponsive hydrogels with controlled poly(ethylene glycol)—poly(propylene glycol) multiblock copolymer structure

New thermoresponsive crosslinked hydrogels with controlled multiblock copolymer structure were prepared from equimolar amounts of α,ω-diamino poly(propylene glycol)s with molecular weights (MW) 230, 400, and 2,000 g mol^?1 and diepoxy-terminated poly(ethylene glycol)s of approximate MW 1,000; 2,000; and 4,000 g mol^?1. Their thermoresponsive character was investigated on the 10–70 °C interval, while the swelling behavior was tested at 21, 37, and 50 °C. All hydrogels displayed temperature sensitivity, but a volume phase transition was noticed only in the case of poly(propylene glycol) (PPG)₂₀₀₀-containing hydrogels. The volume phase transition temperature (T _VPT ) depended on the MW of the hydrophilic poly(ethylene glycol) (PEG) chains attached to the PPG₂₀₀₀ block, as well as on the added salts. Longer PEG blocks determined a shift of T _VPT towards higher values, while the influence of the salt added was in agreement with the Hofmeister series, except for NaH₂PO₄ which determined the destruction of the hydrogel network. The equilibrium swelling degree depended on the MW of both PEG and PPG blocks, as well as on temperature. The analysis of the swelling process indicated a modification of the gel characteristics with temperature and second-order kinetics for the water penetration into the hydrogel.     

Investigation of antibacterial properties silver nanoparticles prepared via green method

ABSTRACT: BACKGROUND: This study aims to investigate the influence of different stirring times on antibacterial activity of silver nanoparticles in polyethylene glycol (PEG) suspension. The silver nanoparticles (Ag-NPs) were prepared by green synthesis method using green agents, polyethylene glycol (PEG) under moderate temperature at different stirring times. Silver nitrate (AgNO3) was taken as the metal precursor while PEG was used as the solid support and polymeric stabilizer. The antibacterial activity of different sizes of nanosilver was investigated against Gram-positive [Staphylococcus aureus] and Gram-negative bacteria [Salmonella typhimurium SL1344] by the disk diffusion method using Mueller-Hinton Agar. RESULTS: Formation of Ag-NPs was determined by UV-vis spectroscopy where surface plasmon absorption maxima can be observed at 412-437 nm from the UV-vis spectrum. The synthesized nanoparticles were also characterized by X-ray diffraction (XRD). The peaks in the XRD pattern confirmed that the Ag-NPs possessed a face-centered cubic and peaks of contaminated crystalline phases were unable to be located. Transmission electron microscopy (TEM) revealed that Ag-NPs synthesized were in spherical shape. The optimum stirring time to synthesize smallest particle size was 6 hours with mean diameter of 11.23 nm. Zeta potential results indicate that the stability of the Ag-NPs is increases at the 6 h stirring time of reaction. The Fourier transform infrared (FT-IR) spectrum suggested the complexation present between PEG and Ag-NPs. The Ag-NPs in PEG were effective against all bacteria tested. Higher antibacterial activity was observed for Ag-NPs with smaller size. These suggest that Ag-NPs can be employed as an effective bacteria inhibitor and can be applied in medical field. CONCLUSIONS: Ag-NPs were successfully synthesized in PEG suspension under moderate temperature at different stirring times. The study clearly showed that the Ag-NPs with different stirring times exhibit inhibition towards the tested gram-positive and gram-negative bacteria.     

Synthesis of Silver Nanoparticles from Extracts of Wild Ginger (Zingiber zerumbet) with Antibacterial Activity against Selective Multidrug Resistant Oral Bacteria

Antibiotic resistance rate is rising worldwide. Silver nanoparticles (AgNPs) are potent for fighting antimicrobial resistance (AMR), independently or synergistically. The purpose of this study was to prepare AgNPs using wild ginger extracts and to evaluate the antibacterial efficacy of these AgNPs against multidrug-resistant (MDR) Staphylococcus aureus, Streptococcus mutans, and Enterococcus faecalis. AgNPs were synthesized using wild ginger extracts at room temperature through different parameters for optimization, i.e., pH and variable molar concentration. Synthesis of AgNPs was confirmed by UV/visible spectroscopy and further characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy analysis (EDXA), and Fourier-transform infrared spectroscopy (FTIR). Disc and agar well diffusion techniques were utilized to determine the in vitro antibacterial activity of plant extracts and AgNPs. The surface plasmon resonance peaks in absorption spectra for silver suspension showed the absorption maxima in the range of 400–420 nm. Functional biomolecules such as N–H, C–H, O–H, C–O, and C–O–C were present in Zingiber zerumbet (Z. zerumbet) (aqueous and organic extracts) responsible for the AgNP formation characterized by FTIR. The crystalline structure of ZZAE-AgCl-NPs and ZZEE-AgCl-NPs was displayed in the XRD analysis. SEM analysis revealed the surface morphology. The EDXA analysis also confirmed the element of silver. It was revealed that AgNPs were seemingly spherical in morphology. The biosynthesized AgNPs exhibited complete antibacterial activity against the tested MDR bacterial strains. This study indicates that AgNPs of wild ginger extracts exhibit potent antibacterial activity against MDR bacterial strains.     

Synthesis and study of water-soluble chitosan-O-poly(ethylene glycol) graft copolymers

Novel chitosan-O-poly(ethylene glycol) graft copolymers were synthesized. Etherification of N-phthaloyl chitosan by poly(ethylene glycol) monomethyl ether (MPEG) iodide was carried out in dimethylformamide in the presence of silver oxide. Varying the ratio of MPEG iodide to chitosan, different degree of O-substitution of MPEG to monosaccharide residue of chitosan (5-197%) was obtained. Chemical structure of the new chitosan derivatives was confirmed using FTIR spectroscopy and analysis of functional groups. O-PEGylated chitosans are soluble in water and aqueous solutions of wide pH range. Reduced viscosity of aqueous solutions of the graft copolymers is extremely low and similar to that of MPEG-2000.     

Synthesis, Characterization, and Antimicrobial Activity of Poly(acrylonitrile-co-methyl methacrylate) with Silver Nanoparticles

Nanotechnology is expected to open some new aspects to fight and prevent diseases using atomic-scale tailoring of materials. The main aim of this study is to biosynthesize silver nanoparticles (AgNPs) using Trichoderma viride (HQ438699); the metabolite of this fungus will help either in reduction of the silver nitrate-adding active materials which will be loaded on the surface of the produced AgNPs. Poly(acrylonitrile-co-methyl methacrylate) copolymer (poly (AN-co-MMA)) was grafted with the prepared AgNPs. The poly(AN-co-MMA)/AgNPs were examined against ten different pathogenic bacterial strains, and the result was compared with another four different generic antibiotics. The produced poly(AN-co-MMA)/AgNPs showed high antibacterial activity compared with the four standard antibiotics. Moreover, the grafting of these AgNPs into the copolymer has potential application in the biomedical field.     

Synthesis of Rumex hastatus-based silver nanoparticles induced the inhibition of human pathogenic bacterial strains

The development of antibiotic resistance in pathogenic bacterial strains has drawn attention to the quest for new natural antibacterial drugs. Therefore, in the present study, extracts of Rumex hastatus leaves were obtained in methanol and water, and R. hastatus-based silver nanoparticles (AgNPs) were synthesized. Structural and functional properties of synthesized silver nanoparticles were determined by UV–vis spectroscopy, XRD, FTIR and SEM. The synthesized AgNPs and crude extracts were tested to check their antibacterial potential against human pathogenic bacterial strains of Staphylococcus aureus, Staphylococcus haemoliticus, Bacillus cereus, Escherichia coli, Salmonella typhi and Pseudomonas aeruginosa in well diffusion and broth dilution methods. The present investigation has revealed for the first time that the broth dilution method was found more reproducible than that of the well diffusion method even at lower concentrations of AgNPs and crude extracts. UV– Vis spectroscopic analysis of AgNPs revealed a peak at 367 nm_. XRD pattern showed a face-centered cubical to the spherical structure of AgNP crystals. FTIR analysis revealed that flavonoids and terpenoids are responsible for the reduction of AgNO₃ to Ag⁺. SEM analysis determined the spherical structure and 51 nm average diameter of nanoparticles. The antibacterial activity of R. hastatus-based (AgNPs) was found to be significantly higher than aqueous plant extract and silver nitrate alone. Bacterial growth was inhibited by R. hastatus-based AgNPs in a dose-dependent manner. To our knowledge, silver nanoparticles (AgNPs) of R. hastatus were synthesized and characterized for the first time in this study and, based on the findings of current research work R. hastatus extract-based silver nanoparticles are suggested to be used as an antibacterial drug instead of synthetic drugs for the treatment of various human diseases/infections caused by the tested bacterial strains.     

Antibacterial Activity and Synergistic Effect of Biosynthesized AgNPs with Antibiotics Against Multidrug-Resistant Biofilm-Forming Coagulase-Negative Staphylococci Isolated from Clinical Samples

Silver nanoparticles form promising template for designing antimicrobial agents against drug resistant pathogenic microorganisms. Thus, the development of a reliable green approach for the synthesis of nanoparticles is an important aspect of current nanotechnology research. In the present investigation, silver nanoparticles synthesized by a soil Bacillus sp. were characterized using UV–vis spectroscopy, FTIR, SEM, and EDS. The antibacterial potential of biosynthesized silver nanoparticles, standard antibiotics, and their conjugates were evaluated against multidrug-resistant biofilm-forming coagulase-negative S. epidermidis strains, S. aureus, Salmonella Typhi, Salmonella Paratyphi, and V. cholerae. Interestingly, silver nanoparticles (AgNPs) showed remarkable antibacterial activity against all the test strains with the highest activity against S. epidermidis strains 145 and 152. In addition, the highest synergistic effect of AgNPs was observed with chloramphenicol against Salmonella typhi. The results of the study clearly indicate the promising biomedical applications of biosynthesized AgNPs.     

Use of Natural Monomer in the Synthesis of Nano- and Microparticles of Polyurethane by Suspension-Polyaddition Technique

Summary: Polyurethane nano- and microparticles were synthesized by suspension-polyaddition technique, using aqueous polymerization medium. Castor oil, a vegetable triglyceride possessing hydroxyl groups was used as natural polyol and methylene diphenyl diisocyanate (MDI) as isocyanate. The levofloxacin, an antibacterial drug was used as model drug to measure the particles encapsulation efficiency. The effect of the addition of a second polyol, the poly(ethylene glycol) (PEG), and the stirring rate on the mean diameter and morphology of particles was also investigated. The poly(ethylene glycol) has an important effect in the reduction of particles size and their porosity. On the other hand, the poly(ethylene glycol) reduced the yield of encapsulation from 70% for the formulation without PEG to 20% for formulations with PEG. FTIR analysis confirmed the polyurethane formation. Dynamic light scattering study, transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were used to determine the nanoparticles size and shape. Spectrofluorimetric analysis was used to detect the levofloxacin.     

An innovative, easily fabricated, silver nanoparticle-based titanium implant coating: development and analytical characterization

Microbial colonization and biofilm formation on implanted devices represent an important complication in orthopaedic and dental surgery and may result in implant failure. Controlled release of antibacterial agents directly at the implant site may represent an effective approach to treat these chronic complications. Resistance to conventional antibiotics by pathogenic bacteria has emerged in recent years as a major problem of public health. In order to overcome this problem, non-conventional antimicrobial agents have been under investigation. In this study, polyacrylate-based hydrogel thin coatings have been electrosynthesised on titanium substrates starting from poly(ethylene glycol diacrylate)–co–acrylic acid. Silver nanoparticles (AgNPs) with a narrow size distribution have been synthesized using a “green” procedure and immobilized on Ti implant surfaces exploiting hydrogel coatings’ swelling capabilities. The coatings have been characterized by XPS and SEM/EDX, while their silver release performances have been monitored by ICP–MS. The antibacterial activity of these AgNP-modified hydrogel coatings was tested evaluating in vitro inhibition growth of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli, among the most common pathogens in orthopaedic infections. Moreover, a preliminary investigation of the biocompatibility of silver-loaded coatings versus MG63 human osteoblast-like cells has been performed. An important point of strength of this paper, in fact, is the concern about the effect of silver species on the surrounding cell system in implanted medical devices. Silver ion release has been properly tuned in order to assure antibacterial activity while preserving osteoblasts’ response at the implant interface.

Controlling size and stabilization of silver nanoparticles for use in optimized chitosan-dialdehyde xylan wound dressings

The size of silver nanoparticles (AgNPs) is the key factor that governs their antibacterial activity. However, the size of AgNPs is difficult to control because agglomeration and uneven dispersion often occur during the processing of AgNP-based products, which has impeded their applications in different areas. In this work, an efficient strategy was developed to overcome this difficulty and to prepare an antibacterial hydrogel comprising AgNPs and chitosan (CS) with dialdehyde xylan (DAX) as the crosslinking agent. The size of AgNPs was controlled successfully to an extremely fine level (<?9 nm) by reducing AgNO₃ solution in a methanolic suspension of the metal organic framework (MOF) -UiO-66-NH₂, and forming an Ag@UiO-66-NH₂ core–shell structure which avoided the agglomeration of AgNPs. DAX played a dual role by forming a hydrogel structure with CS through crosslinking, but also by stabilizing the even dispersion of Ag@UiO-66-NH₂ in the hydrogel. Accordingly, the as-prepared hydrogels showed excellent antibacterial properties and low cytotoxicity. The survival ratio of NIH/3T3 cells cultured in the hydrogel extract was more than 90%, even when the concentration of the hydrogel extract was as high as 10 mg/mL. In addition, the hydrogel exhibited good abilities of water absorption (swelling ratio was up to 1100%) and self-healing (efficiency was up to 88% after 5 h). The hydrogels with size-well-controlled AgNPs prepared in this work are expected to find broad applications, especially in the area of antibacterial medical auxiliaries.

     

Antibacterial poly(ethylene glycol) hydrogels from combined epoxy‐amine and thiol‐ene click reaction

Antibacterial hydrogels containing quaternary ammonium (QA) groups were prepared via a facile thiol‐ene “click” reaction using multifunctional poly(ethylene glycol) (PEG). The multifunctional PEG polymers were prepared by an epoxy‐amine ring opening reaction. The chemical and physical properties of the hydrogels could be tuned with different crosslinking structures and crosslinking densities. The antibacterial hydrogel structures prepared from PEG Pendant QA were less well‐defined than those from PEG Chain‐End QA. Furthermore, functionalization of the PEG‐type hydrogels with QA groups produced strong antibacterial abilities against Staphylococcus aureus, and therefore has the potential to be used as an anti‐infective material for biomedical devices. © 2015 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2016 , 54, 656–667     

Biosynthesis of silver nanoparticle using flowers of Calotropis gigantea (L.) W.T. Aiton and activity against pathogenic bacteria

Silver nanoparticles (AgNPs) from silver nitrate solution are carried out using the flower extract of Calotropis gigantea. Silver nanoparticles were characterized by UV–vis spectrophotometer, X-Ray diffractometer (XRD). Reduction of silver ions in the aqueous solution of silver during the reaction was observed by UV–vis spectroscopy. Crystalline nature of synthesized silver nanoparticles was studied by XRD pattern, refraction peak using the Scherrer’s equation. Antibacterial activity of the silver nanoparticles was performed by disc diffusion method against Bacillus subtilis, Pseudomonas putida and Escherichia coli. The antibacterial activity of synthesized silver nanoparticles by flower extract of C. gigantea was found against B. subtilis (10 mm). Synthesised AgNPs has the efficient antibacterial activity against Gram positive bacteria.     

Green Synthesis of Silver Nanoparticles Using Acacia farnesiana (Sweet Acacia) Seed Extract Under Microwave Irradiation and Their Biological Assessment

In the present study, Acacia farnesiana (Sweet acacia) seed extract is used to reduce Ag⁺ → Ag⁰ under microwave irradiation. The formation of silver nanoparticles (AgNPs) is monitored by recording the UV–Vis absorption spectra for surface plasmon resonance (SPR) peak at ~450 nm. The absorbance of SPR increases linearly with increasing temperature of the reaction mixture. Rapid reduction of silver ions occurred to form AgNPs, 80–90 % yield in about 150 s. A marginal decrease in pH and increase in solution potential (E) of the reaction mixture during the formation of AgNPs are in agreement with the proposed mechanism. XRD pattern of the AgNPs agree with the fcc structure of Ag metal, and the calculated crystallite size is ~17 nm. FT-IR and solid-state ¹³C NMR spectra indicate the functional groups of flavonones and terpenoids (biomolecules from plant extract) which are adsorbed on AgNPs, thereby the present method led to in situ biofunctionalization/bio-capping of AgNPs. TG analysis shows the thermal decomposition of these plant residues present on AgNPs at about 250 °C. The spherical shape of the particles with a diameter (?) in the range of ~15–20 nm is evident from FE-SEM image. Elemental analysis by EDX analysis confirms the presence of Ag as the only major element. The in vitro antibacterial screening of AgNPs shows that these bio-capped AgNPs have higher inhibitory action for E. coli and S. aureus followed by B. subtilis and P. aeruginosa. In addition, AgNPs show very good antioxidant property.     

<Emphasis Type="Italic">Stachys lavandulifolia</Emphasis> and <Emphasis Type="Italic">Lathyrus</Emphasis> sp. Mediated for Green Synthesis of Silver Nanoparticles and Evaluation Its Antifungal Activity Against <Emphasis Type="Italic">Dothiorella sarmentorum</Emphasis>

In this study, silver nanoparticles (AgNPs) were biosynthesized using Stachys lavandulifolia and Lathyrus sp. The first sign of the reduction of silver ions to AgNPs was the change in color of S. lavandulifolia and Lathyrus sp. extracts changed into dark brown and auburn after treating with silver nitrate, respectively. The UV–Vis spectroscopy of reaction mixture (extract+silver nitrate) produced by S. lavandulifolia and Lathyrus sp. showed the strong adsorption peaks at ?440 and 420 nm, respectively. The transmission electron microscope images showed the synthesis of AgNPs using S. lavandulifolia and Lathyrus sp. with an average size of 7 and 11 nm, respectively. The result of X-ray diffraction pattern showed four diffraction peaks at 38°, 44°, 64°, and 77° for both types of biosynthesized AgNPs. Fourier transform infrared spectroscopy showed the possible role of involved proteins and polyhydroxyl functional groups in the synthesis process of AgNPs. Inductively coupled plasma analysis determined the conversion rate (percentage) of silver ions to silver nanoparticles in reaction mixtures of S. lavandulifolia and Lathyrus sp. 99.73 and 99.67 %, respectively. In addition, antifungal effect of AgNPs, synthesized by both extracts, was studied separately on mycelial growth of Dothiorella sarmentorum, in a completely randomized design on potato dextrose agar (PDA) medium. The inhibition rate of mycelial growth was strongly depended on the density of AgNPs and it strongly increased with increasing the density of AgNPs in the PDA medium. AgNPs more than 90 % of them inhibited from the mycelia growth of the fungus at the concentration of 40 µg/mL and higher.