分子模拟用于尤文肉瘤EWS-FLI1蛋白HLA-A2.1限制性CTL表位的筛选

为获得尤文肉瘤EWS-FLI1融合蛋白有效的HLA-A2.1限制性CTL表位, 综合运用BIMAS, SYFPEITHI, Predep和IEDB方案对EWS-FLI1进行CTL表位的预测, 得出8个CTL表位9肽序列; 应用多项式方案、量化基序方案对8个表位9肽进一步进行筛选, 获得其中4个9肽序列, 并进一步运用分子模拟方法初步模拟了4个表位肽与HLA-A2.1分子间的相互结合作用; 应用标准Fmoc方案合成CTL表位肽, RP-HPLC与MS分别鉴定合成肽的纯度与分子量; 通过亲和力实验验证了表位肽QIQLWQFLL_{(EWS-FLI1 304)}与HLA-A2.1有较强的结合力, 从而为表位肽的后继免疫学特性研究提供了基础. 本研究提供了一个合理高效的表位筛选方法.     

Absorption and emission spectra in chalcogenide glass of the composition 0.7Ga2S3·0.27La2S3·0.03Nd2S3

Chalcogenide glasses of the composition 0.7Ga₂S₃·0.27La₂S₃·0.03Nd₂S₃ doped by 3 mol% of Nd³⁺ were prepared. The absorption and emission spectra of these glasses were compared to those of commercial ED-2 3.1 wt.% Nd-doped silicate glass. The absorption intensities of Nd³⁺ in chalcogenide glass are higher than in silicate glass due to the increasing covalency of Nd³⁺ in these glasses. Because of the low phonon frequency of the chalcogenide glasses nonradiative relaxations from the ⁴F_{$\text{5}\text{2}$}, ²H_{$\text{9}\text{2}$} to the ⁴F_{$\text{3}\text{2}$} are lower than in other oxide glasses and so fluorescence from this state is observed.     

在重组痘苗病毒中共表达HIV-1p24gag与hIL-6基因

以ＨＩＶ－１ ｐ２４ｇａｇ 与ｈＩＬ－６ 基因在重组痘苗病毒中的共表达研究为目的,将痘苗病毒复制非必需区血凝素（ＨＡ） 基因作为侧翼,把编码人白细胞介素６（ｈＩＬ－６）的基因片段克隆到真核表达质粒ｐ１６ＱＦ１ －２４ 的下游,构建成含有ＨＩＶ－ １ｐ２４ｇａｇ 和ｈＩＬ－６ 两种外源基因的重组表达质粒ｐ１６ＱＦ２４ －ＩＬ６ ,经同源重组和血凝素阴性空斑筛选,获得了重组痘苗病毒ｖ１６ＱＦ２４ －ＩＬ６．经间接免疫荧光试验、Ｄｏｔ－ ＥＬＩＳＡ 和Ｗｅｓｔｅｒｎ ｂｏｌｔ 等检测证明,重组病毒能同时表达ｐ２４ｇａｇ 蛋白和ＩＬ－６ 蛋白,表达产物的相对分子质量分别为２－４×１０４ ,２－６×１０４ ．此种方式表达的外源蛋白持续释放,可产生更强的免疫效果,可望用于ＨＩＶ疫苗的研究．     

The preparation of cobalt complexes containing simple sulphur-nitrogen ligands,S2N2H− and S3N−, from S4N4O2

Summary Reactions of Group VIII metal halides [CoCl₂, NiCl₂, K₂PdCl₄, PdCl₂(PhCN)₂ and PtCl₂] with tetrasulphurtetranitrogendioxide, S₄N₄O₂, have been investigated. The cobalt reaction yields Co(S₂N₂H)₂, Co(S₂N₂H)(S₃N) and Co(S₃N)₂. The usefulness of metal centres for trapping reactive sulphur-nitrogen centres is discussed.     

高硫合成气制甲硫醇钼硫基催化剂的制备

钼硫基催化剂;含硫化氢合成气;高硫合成气制甲硫醇钼硫基催化剂的制备     

Synthesis and study of new rod-like mesogens containing 2-aminothiophene unit

We present a synthesis and mesomorphic properties of a new series of rod-like mesogens. All the compounds possess a substituted 2-aminothiophene unit as a main element in the structure attached to a stilbene moiety with a terminal alkyloxy chain (OR¹, OR² where R¹=C_nH_2n+1, R²=C_mH_2m+1; n, m ranging from 6 to 12). The synthesis of alkyloxybiphenyl substituted 2-aminothiophenes was carried out by the Gewald reaction and the appropriate reaction conditions were investigated. The liquid–crystalline properties were studied via polarizing optical microscopy, differential scanning calorimetry and X-ray diffraction. These materials exhibit nematic and/or smectic A phases. The influence of structural changes (variation in alkyloxy chain length and symmetry of the molecule) on mesogenic behaviour is discussed. Evaluation of UV–vis, fluorescent and electrochemical properties are also included.     

Application of heparinized cellulose affinity membranes in recombinant adeno-associated virus serotype 2 binding and delivery

The microporous affinity membrane based on cellulose matrices offers minimal mass-transfer effects in membrane chromatography with low nonspecific adsorption. In this research, we tested a novel application of the microporous, heparinized cellulose membranes for their affinity toward recombinant adeno-associated virus serotype 2 (rAAV2, which uses heparan sulfate proteoglycans as the primary cellular receptor) to develop a controlled, substrate-mediated viral vector delivery. We conjugated rAAV2 to an epoxy-crosslinked heparin cellulose membrane, which led to vector transduction upon cellular adhesion. When adhered, human fibroblasts exhibited proliferation kinetics similar to those on the standard polystyrene tissue-culture surface. Using fluorescent proteins as the reporter, we showed that the heparin-bound rAAV2 particles remained active and that the rAAV2-heparin binding was reversible and capable of mediating transgene delivery in cell culture. In addition, we applied the affinity membrane to adsorb unpurified rAAV2 from the crude lysate of packaging cells via the ligand–receptor binding, avoiding the use of conventional ultracentrifugation or chromatography in preparation of infectious rAAV2 for transduction. Our work explores a new application of affinity cellulose membranes in substrate-mediated viral vector delivery, which can be a useful tool in developing protocols for localized gene transfer.     

Rheological study of blood cells containing haemoglobin S. Physical interpretation of viscosity laws

Starting from experimental results given in the literature, changes in viscosity with haematocrit on suspensions of HbS erythrocytes or mixtures of normal HbA and HbS cells, when deoxygenated, are obtained. Laws of an exponential type are derived and the notion of the haematocrit constant playing an important part in the cell-cell interaction is pointed out. Its physical significance corresponds to a variation in the activation energy between the solution and the solvent just equalling the thermal energy.     

Oxidation of aliphatic alcohols by a system containing Na2S2O8 and a metal chloride

Conclusions The oxidation of primary aliphatic alcohols by the Na₂S₂O₈-alkali metal chloride system in water in the presence of sulfuric acid gives 1,1-dialkoxy-2-chloroalkanes.Translated from Izvestiya Akademii Nauk SSSR, Seriya Khimicheskaya, No. 8, pp. 1932–1934, August, 1988.     

Computational study of the properties and reactions of small molecules containing O, S, and Se

The reactions and properties of a series of chalcogen-containing compounds (CH(3))(2)X and (CH(3))(2)C═X, where X = O, S, and Se, were studied computationally at the CBS-QB3 level to examine the differences among these molecules. The reactions and properties investigated include the double bond dissociation energy, the ionization potential, the interaction energies with a series of acids including a proton, CH(3)(+), Li(+), MeLi, and MeOH, and the enolization energies of the (CH(3))(2)C═X species. The effect of substituting the O of acetamide with S or Se also was studied. The changes that result from these reactions were examined via changes in structure and changes in charge distribution using the Hirshfeld charges.