共查询到20条相似文献,搜索用时 0 毫秒
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G T Hill 《Journal of chromatography. A》1979,176(3):407-412
A high-performance liquid chromatographic method for the analysis of prostacyclin using a laboratory prepared reversed-phase column packing is described. A relative standard deviation of less than 1% was obtained for ten replicate injections. The system resolves prostacyclin from its hydrolysis product, 6-oxo-prostaglandin F1 alpha and from other prostaglandins present as impurities. These can be estimated to levels of approximately 0.5%. The separation of other unrelated prostaglandins by this method is briefly reported. 相似文献
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A fast, selective, and precise liquid chromatographic method for simultaneous, independent determination of kanamycins A and B is described. Sample components are separated on a pellicular cation exchanger and monitored by fluorescence using post-column on-line derivatization. Less than 0.35 mug of kanamycin B can be detected in as much as 7 mug kanamycin A injected. The detection limit for kanamycin A is less than 20 ng injected. Reproducibility of the entire chromatographic system is about 1% (2 theta) based upon repeated injections of standards. Precision of repeated process sample preparation is about 6% (2 theta). Chromatographic analysis time is less than 15 min per sample. 相似文献
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A rapid and specific ion-pair reversed-phase high-performance liquid chromatographic method was developed for the determination of bleomycins. The use of 5-microns particles of less adsorptive reversed-phase packings and sodium perchlorate as ion-pairing reagent permitted a short analysis time and the transferability of the separations on different batches of the reversed-phase materials. The detection sensitivity and precision of the method demonstrated that the system is suitable for routine analysis. 相似文献
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Summary Busulfan (Myleran; 1,4-bis-(methanesulfonyloxy) butane; BU) is a bifunctional alkylating agent used in clinical practice since
1959. It is currently included at high doses in conditioning regimens for bone marrow transplantation, usually in combination
with cyclo-phosphamide. A high-performance liquid chromatographic method has been developed for the determination of BU in
plasma. The basis of the assay is a derivatization with sodium diethyldithiocarbamate at 32°C in the presence of 1-bromo-1-deoxy-3,6-anhydrogalactitol
as internal standard. Analysis is performed on a cyano column with heptane-isopropanol-glacial acetic acid as mobile phase
and UV detection at 280 nm. The calibration graph was linear in the concentration range 0.18–46.40 μM BU in plasma. The limit
of detection was 0.1 μM. The precision and accuracy were between the limits required by good laboratory practice.
Presented at Balaton Symposium on High-Performance Separation Methods, Siófok, Hungary, september 1–3, 1999 相似文献
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High-performance liquid chromatographic determination of histamine N-methyltransferase activity 总被引:1,自引:0,他引:1
H Fukuda A Yamatodani I Imamura K Maeyama T Watanabe H Wada 《Journal of chromatography. A》1991,567(2):459-464
A method for the determination of histamine N-methyltransferase (HMT) activity by high-performance liquid chromatography based on post-column derivatization with omicron-phthalaldehyde is described. The determination involves the separation of the substrate, histamine, from its product. N tau-methylhistamine, using a weak cation exchanger, followed by on-line derivatization of these imidazoleamines with omicron-phthalaldehyde and their detection and quantitation with a fluorimetric detector. This assay method is suitable for the measurement of HMT activity during enzyme purification. 相似文献
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Sánchez-Machado DI López-Hernández J Paseiro-Losada P 《Journal of chromatography. A》2002,976(1-2):277-284
A high-performance liquid chromatographic (HPLC) method for the microscale determination of alpha-tocopherol in macroalgae is reported. The method includes microscale saponification and extraction with n-hexane. The presence of alpha-tocopherol in macroalgae samples was confirmed by HPLC-MS. Alpha-tocopherol levels as determined in samples by HPLC with UV and fluorescence detection did not differ significantly; however, fluorescence detection has a higher sensitivity (detection limit 10.4 ng/ml, vs. 104 ng/ml with UV detection), as well as good precision (relative standard deviation 1.81%) and recovery (94.3%). Fluorescence detection is also faster. We used this method to determine the alpha-tocopherol contents of four commercial macroalgae products from northwest Spain as part of nutritional studies in dehydrated Himanthalia elongata and Laminaria ochroleuca, and also in canned Himanthalia elongata and Saccorhiza polychides. 相似文献
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Lyle D. Bighley Dale E. Wurster Diana Cruden-Loeb Robert V. Smith 《Journal of chromatography. A》1975,110(2):375-380
A sensitive analysis of pentaerythritol in plasma has been devised, based on the formation or its tetra-p-methoxybenzoate derivative and high-performance liquid chromatography employing an ultraviolet photometric detector. The method permits analysis of pentaerythritol in the ppm range. 相似文献
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Accurate quantitation of the four lipid components in a vesicle formulation has been accomplished by selectively changing the high-performance liquid chromatographic mobile phase composition to arrive at an optimal separation requiring less than 10 min. A systematic approach to selection of the mobile phase conditions has minimized the number of experiments required to achieve the necessary separation. Relative standard deviations between one and four percent are obtained for the four lipid components. Accuracy, as measured by recovery experiments for spiked vesicle formulations, varies between one and two percent of target levels at the nominal lipid concentrations investigated. 相似文献