Distinguishing Structure Change of Cells Based on Analysis of Light Scattering Patterns

We develop a new method to distinguish structural change of cells based on light scattering and Fourier spectra analysis. The light scattering detection system is composed of a laser source, an optical microscope, a CCD with high resolution and low distortion. After the scattering patterns of cells are recorded by the CCD, the Fourier spectra are obtained by the intensity distribution of scattered light. In the experiment, the change of cell structure is designed by sonication treatment. It is found that different typical peaks can be shown in the Fourier spectra of MCF7 cells with and without sonication treatment, which indicates that this method can be used to distinguish the structural change of cells.     

Wave scattering by many small particles embedded in a medium

Theory of wave scattering by many small bodies is developed under various assumptions concerning the ratio , where a is the characteristic dimension of a small body and d is the distance between neighboring bodies d=O(aκ₁), 0<κ₁<1. On the boundary Sm of every small body an impedance-type condition is assumed on Sm, 1?m?M, ζm=hma−κ, 0<κ, hm are constants independent of a. The behavior of the field in the region in which M=M(a)?1 small particles are embedded is studied as a→0 and M(a)→∞. Formulas for the refraction coefficient of the limiting medium are derived under the assumptions: (a) κ₁=(2−κ)/3, 0<κ?1, and (b) κ₁=1/3, κ>1. A method for creating materials with a desired refraction coefficient is proposed and justified theoretically on the basis of the above results.     

Polarization-Independent Directional Beaming of Light by a Subwavelength Metal Slit

We present the directional beaming effect of light at the terahertz frequency by using a subwavelength slit in the metal film. The metal is dressed with anisotropic dielectric so that both the transverse electric （TE） and transverse magnetic （TM） polarized waves can be well guided on the metal surface and reach the phase matching. By using a periodical array of dielectric ridges and grooves around the slit, the guided waves can be scattered out of the slit and interfere with the transmitted light directly through the slit. The results performed by finite-difference at time-domain computations indicate that the directional beaming of light can be obtained simultaneously for both the TE and TM polarized waves after optimizing the geometric parameters. The structure may find great applications in polarization-independent optical devices such as couplers, connectors, beam collimator, and etc.     

Isothermal depolarization currents in triglycine sulphate

5 s; the polarizing field was varied from 10 V/cm to 10 kV/cm, and the temperature between -190 °C and 25 °C. The results confirm that the depolarization current in TGS follows the formula I_d∼[(ω_pt)ⁿ+(ω_pt)^m]^-1, where n<1 and m>1. The values of the parameters n and m depend on both the electric field strength and the polarization time as well as on the temperature of the experiment. The loss peak frequency ω_p exhibits activation character: ω_p∼exp(-E/k_BT) with E=0.34 eV at temperatures directly below room temperature and E=0.013 eV in the range of liquid nitrogen temperatures. Received: 1 April 1997/Accepted: 7 April 1997     

Experimental determination of the multiple-scattering effect on the lidar-signal polarization characteristics during liquid- and solid-phase precipitation

We present results of experimental investigations of the signal-polarization characteristics in the case of lidar sounding during precipitation. We show and discuss the lidar signals and the depolarization profiles along the sounding path for liquid- and solid-phase precipitation. In the former case we compare the signal characteristics at different degrees of precipitation rate. In the latter situation, we consider snowfall with particle shape close to that of Chebyshev particles. We also follow the lidar-signal changes depending on the field-of-view of the receiving optics. The experimental data are compared with results of theoretical estimates and models concerning the optical and microphysical characteristics of the rain and snow particles. In the case of liquid-phase precipitation – rain – the observed dependence of the lidar’s signal-polarization structure on the precipitation intensity has two aspects: on the one hand, the change of the raindrops’ shape, and, on the other, the multiple-scattering effects. The lidar data demonstrate that the signal depolarization, and, more specifically, its behavior along the sounding path, can be used as a criterion for the presence of multiple scattering. In the case of a snowfall consisting of Chebyshev particles, the simultaneous role is evident of two factors influencing the lidar-signal depolarization, namely, the non-spherical shape of the particles and the multiple-scattering effects. When the scattering takes place off particles with a large size and a shape strongly differing from spherical, we observed the predominant role of the non-sphericity of the scattering centers in the signal depolarization. Received: 6 December 2000 / Revised version: 11 July 2001 / Published online: 19 September 2001     

Dependence of the lidar signal depolarization on the receiver’s field of view in the sounding of fog and clouds

We report an experimental study of the lidar signal depolarization as a function of the relative contribution of the multiple scattering in case of optically dense objects in the atmospheric planetary boundary layer. Results of the observation of fog and stratus clouds are presented, as well as those obtained by sounding of stratocumulus clouds during a snowfall. The lidar data point to a rise of the depolarization coefficient as the influence of the multiple scattering increases in consequence of both viewing angle enlargement and penetration into the object sounded. The variations of the depolarization coefficient are studied as a function of the field of view. In the case of fog, this dependence is approximated by a three-parameter exponential law; it is found that the depolarization increases steeply when the viewing angle is increased from 9 mrad to 12.5 mrad. The relationships between the approximation parameters and the microphysical characteristics of the scattering medium are considered. The experimentally determined size of the area where multiple scattering occurs is in good agreement with that calculated according to the diffusion model. The results obtained on the multiple scattering effect on the depolarization can also be employed in determining the extinction coefficient profiles in optically dense objects, as well as in evaluating the characteristic size of the scattering particles. Received: 6 September 1999 / Revised version: 7 February 2000 / Published online: 6 September 2000     

A four-wavelength depolarization backscattering LIDAR for polar stratospheric cloud monitoring

A four wavelength backscattering depolarization LIDAR designed for polar stratospheric cloud and stratospheric aerosol measurement is described. The system uses the following wavelengths: 355 nm, 532 nm, 750 nm, and 850 nm. These wavelengths, obtained by means of the third- and second-harmonic of a Nd: YAG laser and by means of a tunable Ti: Sapphire laser, are chosen in a way to better characterize the particel size of such stratospheric aerosols. They are not emitted simultaneously as the LIDAR system is designed with only two detection channels permitting to detect, in the analog and in the photon counting mode, both the direct and the depolarized backscattered signal. The system has been operational in northern Finland since the end of November 1991.     

Study of atmospheric water in gaseous and. liquid state by using combined elastic–Raman. depolarization lidar

A combined elastic–Raman lidar system based on a tripled Nd:YAG laser is used for the separate detection of elastic backscatter and Raman signals from atmospheric nitrogen, water vapor and liquid water and for their depolarization measurement. Vertical profiles of water-vapor and liquid-water content measured under clear-sky conditions behave differently: inside the boundary layer the ratio of liquid-water to water-vapor Raman backscatters rises with altitude. The depolarization measurements bring additional information about atmospheric scattering. The observed depolarization ratio of the water-vapor Raman signal is about 14%, while for liquid water this ratio varies in the 30–75% range, which exceeds the depolarization of bulk water and is attributed to the water-aerosol effects. Raman contours of water vapor and liquid water are partially overlapped, and bleed-through of liquid-water Raman backscatter leads to enhancement of depolarization of the water-vapor Raman signal. This parameter may be used as a convenient indicator of liquid-water interference in water-vapor measurements. Received: 12 December 2000 / Revised version: 27 September 2001 / Published online: 7 November 2001     

Electromagnetic wave scattering by small bodies

A reduction of the Maxwell's system to a Fredholm second-kind integral equation with weakly singular kernel is given for electromagnetic (EM) wave scattering by one and many small bodies. This equation is solved asymptotically as the characteristic size of the bodies tends to zero. The technique developed is used for solving the many-body EM wave scattering problem by rigorously reducing it to solving linear algebraic systems, completely bypassing the usage of integral equations. An equation is derived for the effective field in the medium, in which many small particles are embedded. A method for creating a desired refraction coefficient is outlined.     

Extraordinary Resonant Scattering in Imperfect Acoustic Cloak

We present a systematic study on the extraordinary resonant scattering in imperfect acoustic cloak by means of acoustic scattering theory. Analysis results demonstrate that the resonances are inevitable due to the perturbation to the ideal clo~k, and specific resonance modes are excited by specific order waves. The strength of resonance is determined by the magnitude of perturbation and each order wave＇s sensitivity to the perturbation. Further studies reveal the unique scattering characters of different resonance modes.     

Silver-island films probed by hyper-Rayleigh scattering and atomic force microscopy

Incoherent second-harmonic generation, or hyper-Rayleigh scattering (HRS), and atomic force microscopy (AFM) are proposed as a combined probe of nonlinear optical and structural properties of silver-island films. HRS and linear (Rayleigh) scattering indicatrices are measured. The correlation function, deduced from the HRS indicatrices and characterising spatial fluctuations of the total polarisation at second-harmonic frequency, has a length scale significantly larger than that of the correlation functions that are obtained from the AFM data and characterise spatial fluctuations of quadratic optical susceptibilities of the dipole and quadrupole types. This difference is interpreted as indicating that the HRS indicatrix shape is determined by the long-range fluctuations of the local-field factors. Received: 16 October 2001 / Revised version: 16 April 2002 / Published online: 6 June 2002     

Short pulse laser microforming of thin metal sheets for MEMS manufacturing

Continuous and long-pulse lasers have been used for the forming of metal sheets for macroscopic mechanical applications. However, for the manufacturing of micro-electro-mechanical systems (MEMS), the applicability of such type of lasers is limited by the long-relaxation-time of the thermal fields responsible for the forming phenomena. As a consequence of such slow relaxation, the final sheet deformation state is attained only after a certain time, what makes the generated internal residual stress fields more dependent on ambient conditions and might make difficult the subsequent assembly process for MEMS manufacturing from the point of view of residual stresses due to adjustment.The use of ns laser pulses provides a suitable parameter matching for the laser forming of an important range of sheet components used in MEMS that, preserving the short interaction time scale required for the predominantly mechanic (shock) induction of deformation residual stresses, allows for the successful processing of components in a medium range of miniaturization but particularly important according to its frequent use in such systems.In the present paper, a discussion is presented on the specific features of laser interaction in the timescale and intensity range needed for thin sheet microforming with ns-pulse lasers along with relevant modelling and experimental results and a primary delimitation of the parametric space of the considered class of lasers for the referred processes.     

Scattering and extinction of evanescent waves by small particles

Received: 14 May 1998/Revised version: 31 July 1998     

Multiply scattered component of lidar returns

The results of statistical simulation of the spatiotemporal structure of the multiply scattered component of lidar returns by the Monte Carlo method are discussed for the case of monostatic sensing geometry. The spatial characteristics of the region of the medium where occurs the last scattering of photon before arriving at the reciever. This region of the medium is called the instantaneous brightness body of multiply scattered radiation. It is demonstrated that the instantaneous brightness body of multiply scattered radiation that propagates toward the receiver may occupy a large volume that does not necessarily coincide with the region of formation of the singly scattered component. The main factors influencing the spatial and brightness characteristics of this volume source are established. The effect of scattering order on the spatiotemporal structure of lidar returns is analyzed for the case of sensing of aerosol haze and advective and radiative fogs with optical thickness 2<τ<8. Received: 2 August 2001 / Revised version: 7 January 2002 / Published online: 25 September 2002 RID="*" ID="*"Corresponding author. Fax: +7-38/2225-8026, E-mail: belov@iao.ru     

Surface wave effect on light scattering from one-dimensional photonic crystals

Light scattering and reflection measurements using attenuated total reflection technique for s polarization of the incident light on a finite one-dimensional photonic crystal are reported. Angular specular reflection was measured experimentally to determine, the optimum thickness, the angular position of the surface mode, and the number of bi-layers of the system. It was demonstrated that the position of the surface mode inside the last film is close to the asymptotic value when the number of periods is increased. Spectral reflection measurements were made to determine experimentally the band gap width and measure the dispersion relation of the surface mode inside this band. The corresponding field amplitude was calculated (∣E∣²) showing that in resonant conditions it has a maximum near the surface. The angular dependence of the scattered light measured displays a peak caused by singles cattering and located approximately at the excitation angle of the surface electromagnetic mode. When the incident light is in resonance with the surface electromagnetic wave, it is found experimentally that the scattering of light is enhanced overall by approximately one order of magnitude in comparison with the off-resonance case.     

Direct measurement of the Rayleigh scattering cross section in various gases

Using the laser-based technique of cavity ring-down spectroscopy extinction measurements have been performed in various gases straightforwardly resulting in cross sections for Rayleigh scattering. For Ar and N₂ measurements are performed in the range 470-490 nm, while for CO₂ cross sections are determined in the wider range 470-570 nm. In addition to these gases also for N₂O, CH₄, CO, and SF₆ the scattering cross section is determined at 532 nm, a wavelength of importance for lidar applications and combustion laser diagnostics. In O₂ the cross section at 532 nm is found to depend on pressure due to collision-induced light absorption. The obtained cross sections validate the cross sections for Rayleigh scattering as derived from refractive indices and depolarization ratios through Rayleigh's theory at the few %-level, although somewhat larger discrepancies are found for CO, N₂O and CH₄.     

Simulations of magnetic microstructure in thin film elements used for programmable motion of magnetic particles

The results of two-dimensional micromagnetic modeling of magnetization patterns in Permalloy ellipses under the influence of rotating constant-amplitude magnetic fields are discussed. Ellipses of two different lateral sizes have been studied, 0.5 μm×1.5 μm and 1 μm×3 μm. The amplitude of the rotating magnetic field was varied between simulations with the condition that it must be large enough to saturate or nearly saturate the ellipse with the field applied along the long axis of the ellipse. For the smaller ellipse size it is found that the magnetization pattern forms an S state and the direction of the net magnetization lags behind the direction of the applied field. At a critical angle of the rotating magnetic field the direction of the magnetization switches by a large angle to a new S state. Both the critical angle and the angle interval of the switch depend on field amplitude. For this new state, it is instead the applied field direction that lags behind the magnetization direction. The transient magnetization patterns correspond to multi-domain patterns including two vortices, but this state never exists for the equilibrated magnetization patterns. The behavior of the larger ellipse in rotating field is different. With the field applied along the long-axis of the ellipse, the magnetization of the ellipse is nearly saturated with a vortex close to each apex of the ellipse. As the field is rotated, this magnetization pattern remains and the net-magnetization direction lags behind the direction of the field until for a certain angle of the applied field an equilibrium multi-domain state is created. Comparisons are made with corresponding experimental results obtained by performing in-field magnetic force microscopy on Permalloy ellipses.     

Polarized angular dependence of three-dimensional light-scattering for nanoparticles on thin film wafer

Bidirectional ellipsometry has been developed as a technique for distinguishing among various scattering features near surfaces. The polarized angular dependence of three-dimensional light-scattering by the nanoparticles on thin film wafer is calculated and measured. These calculations and measurements yield angular dependence of bidirectional ellipsometric parameters for out-of-plane light-scattering. The experimental data show good agreement with theoretical predictions for different nanoparticle diameters and thin film thicknesses when bidirectional ellipsometry was employed to measure nanoparticles (60 nm, 100 nm, and 200 nm) on Si wafers with different film thicknesses of 2 nm, 5 nm, and 10 nm. Not only are the diameters of the nanoparticles determined, but also the film thicknesses can be calculated and distinguished from the measurement results. Additionally, the results indicate that improved accuracy is possible for measurements of scattering features from nanoparticles and thin films.     

Effect of Sound Source Scattering on Measurement of Near-Field Head-Related Transfer Functions

A simple spherical head and pulsating spherical sound source model are proposed to investigate the effect of multiple scattering between the head and the sound source on near-field head-related transfer function (HRTF) measurement. Multipole expansion method is used to calculate HRTFs of the model, then the relationships among the magnitude error of HRTF with frequency, source direction, source size, and the distance between the head centre and the sound source are analysed. The results show that to ensure the magnitude error of HRTF within 1.0dB up to 20kHz, for source distance not less than 0.15m or 0.20m, the radius of the sound source should not exceed 0.03m or 0.05m, respectively. The conclusion suggests an appropriate size of sound source in near-field HRTF measurement.