石墨烯修饰电极电化学发光分析法测定甲巯咪唑

基于碱性介质中鲁米诺在石墨烯修饰玻碳电极(GCE)表面的弱电化学发光信号可被少量甲巯咪唑显著增敏的原理建立了一种灵敏测定甲巯咪唑的电化学发光新方法。实验考察了反应介质、石墨烯用量、鲁米诺浓度及电化学扫描速率对体系电化学发光信号的影响。结果发现:在8. 0μL的石墨烯用量、0. 01 mol·L~(-1)NaOH、0. 7μmol·L~(-1)鲁米诺及100 mV/s的扫描速率的优化条件下,甲巯咪唑浓度在6. 0×10~(-8)～1. 0×10~(-5)mol·L~(-1)范围内与其增敏的电化学发光强度呈良好的线性关系,检出限为2. 0×10~(-8)mol·L~(-1),其相对标准偏差(RSD)为3. 5%(c=0. 5μmol·L~(-1),n=11)。该方法可用于甲巯咪唑含量的临床测定,结果较为满意。     

流动注射电化学发光分析法测定间苯三酚

基于间苯三酚对鲁米诺在铂电极上弱的电氧化发光信号的强增敏作用与流动注射技术的结合 ,建立了一种测定间苯三酚的电化学发光新方法。该法测定间苯三酚的检出限为 1.2× 10 - 4g L ;线性范围为 4 .0× 10 - 4～ 4 .0× 10 - 2 g L ;相对标准偏差为 4 .7%。     

电化学发光分析法测定氨基硫脲

基于在碱性介质中,氨基硫脲对罗丹明B的电化学发光具有抑制作用,建立了测定氨基硫脲的电化学发光新方法。运用阶跃脉冲电解方式,在最佳实验条件下,相对电化学发光强度与氨基硫脲的浓度在1.0×10-10～1.0×10-6mol/L范围内呈线性关系,检出限为8.0×10-11mol/L,该法可应用于水样中氨基硫脲含量的测定。     

流动注射电化学发光分析法测定氨苄西林

基于氨苄西林对鲁米诺在铂电极上弱的电氧化发光信号的强增敏作用与流动注射技术的结合,建立了一种测定氨苄西林的电化学发光分析新方法。该法测定氨苄西林的检出限为5.0×10-8g mL,线性范围为8.0×10-8～5.0×10-5g mL,相对标准偏差为2.0%(n=11)。已成功地用于样品中氨苄西林的测定。     

流动注射电化学发光分析法测定甲磺酸培氟沙星

甲磺酸培氟沙星为第三代喹诺酮类抗菌药,疗效好,抗菌谱更广,且对青霉素、头孢菌素、氨基苷类耐药菌株均有效,在临床上被广泛使用。本文发现,在Na2CO3-NaHCO3碱性溶液中,1．40V电解电位下,鲁米诺在铂电极上的弱电化学发光信号可以被甲磺酸培氟沙星强烈地增敏,据此建立了甲磺酸培氟沙星的流动注射电化学发光分析方法。     

流动注射电化学发光分析法测定琥乙红霉素

基于琥乙红霉素对鲁米诺在铂电极上弱的电氧化发光信号的强增敏作用与流动注射技术的结合,建立了一种测定琥乙红霉素电化学发光分析方法。该法测定琥乙红霉素的检出限为7.2×10-6g.L-1,线性范围为8.0×10-6~2.8×10-3g.L-1,相对标准偏差为2.4%(n=11)。方法已用于利君沙片剂中琥乙红霉素的测定。     

流动注射电化学发光分析法测定左旋多巴的研究

基于左旋多巴对鲁米诺在铂电极上弱的电氧化发光信号的强增敏作用与流动注射技术的结合,建立了一种测定左旋多巴的电化学发光分析新方法。该法测定左旋多巴的检出限为2.0×10-10g/mL,线性范围为4.0×10-10~2.0×10-6g/mL,相对标准偏差为2.0%(n=11)。该法简单、快速、灵敏,已成功地用于样品中左旋多巴的测定。     

流动注射电化学发光分析法测定诺氟沙星的研究

基于诺氟沙星对鲁米诺在铂电极上弱的电氧化发光信号的强增敏作用与流动注射技术的结合 ,建立了一种测定诺氟沙星的电化学发光分析新方法。该法测定诺氟沙星的检出限为 4 .0× 10 -6g/L ;线性范围为 1.0× 10 -5～ 0 .2g/L ;相对标准偏差为 1.2 % (n =11)。该法简单、快速、灵敏 ,已成功地用于药物制剂和尿样中诺氟沙星的测定。     

电化学发光法测定铁

基于Fe(o Phen)33 对鲁米诺电化学发光体系的电还原发光信号的增敏作用,建立了一种测定铁离子的电化学发光新方法。在最佳的实验条件下,该方法测定铁(Ⅲ)的线性范围为4.1×10-7～4.1×10-6mol/L,检出限为1.8×10-7mol/L,相对标准偏差小于5%。     

异烟肼在多壁碳纳米管修饰电极上的电化学行为及电化学测定

报道了一种测定异烟肼的化学修饰电极和电分析方法。与裸玻碳电极相比,多壁碳纳米管修饰玻碳电极显著提高异烟肼的氧化峰电流。优化了底液、pH值、修饰剂量、富集电位和富集时间等测定条件,建立了一种直接测定异烟肼的高灵敏度电分析方法。该方法测定异烟肼的线性范围为4×10-7~5×10-5mol/L;富集60 s后的检出限为1.5×10-7mol/L;对5×10-6mol/L的异烟肼平行测定10次的相对标准偏差为4.9%。此方法成功用于异烟肼片剂和血清中异烟肼含量的测定。     

基于离子缔合效应电化学发光分析法测定十二烷基苯磺酸钠

研究发现,丁基罗丹明B与十二烷基苯磺酸钠(SDBS)在0.02mol/LHCl介质中形成的疏水性离子缔合物,可被选择性地萃取到以液体石蜡为粘合剂的碳糊电极表面。当该电极置于0.1mol/LNaOH介质且施加1.3V电解电位时,富集于碳糊电极表面的丁基罗丹明B可被电化学氧化而产生强烈的电化学发光信号,且电化学发光强度与SDBS的浓度成正比。据此,建立了一种选择性好、灵敏度高的测定SDBS的电化学发光新方法。在最佳实验条件下,相对电化学发光强度与SDBS的浓度在2.0×10-6~8.0×10-3g/L范围内呈线性关系,其线性回归方程为ΔIECL=5.1c 44.3,检出限为5.0×10-7g/L,相关系数为0.9983。     

基于电还原鲁米诺电化学发光分析法测定水样中钼(Ⅵ)

基于钼 对鲁米诺电化学发光体系的电还原发光信号的增敏作用 ,建立了一种测定钼 的电化学发光新方法。在最佳的实验条件下 ,相对电化学发光强度与钼 的浓度在 5 .0× 1 0 - 4～ 1 .0× 1 0 - 2 g L范围内呈线性关系 ;检出限为 0 .1 3mg L ;相关系数为 0 .992 0。     

Flow Injection Chemiluminescence Determination of Isoniazid Using On-Line Electrogenerated Manganese(III) as Oxidant

A novel flow injection chemiluminescence (CL) system for the determination of isoniazid has been proposed. It is based on the direct CL reaction of isoniazid and Mn(III) in sulfuric acid medium. The unstable Mn(III) was on-line electrogenerated by constant current electrolysis. The CL emission intensity was linear with isoniazid concentration in the range 0.1–10 μg/mL; the detection limit was 3.2 × 10⁻² μg/mL. The whole process could be completed in 1 min with a relative standard deviation of less than 5%. The proposed method is suitable for automatic and continuous analysis and has been applied successfully to the analysis of isoniazid in pharmaceutical preparation.     

Energy Transfer Electrogenerated Chemiluminescence for the Determination of Sulfite

A simple and novel electrogenerated chemiluminescence (ECL) method for the determination of sulfite has been developed based on the energy transfer ECL process. It was found that a weak ECL signal of sulfite was electrochemically generated on a platinum electrode in neutral aqueous solution. The signal was strongly enhanced by rhodamine B as an energy receptor and further enhanced by the neutral surfactant Tween 80. In 0.10M phosphate buffer solution (pH=7.5) containing 2.0×10^–6gmL^–1 rhodamine B and 0.4% (v/v) Tween 80, the ECL response to the concentration of sulfite at a potential of 0.82V was linear over a range of 1.0×10^–7gmL^–1 to 8.0×10^–6gmL^–1, and the detection limit was 5×10^–8gmL^–1. The relative standard deviation (n=11, 1.0×10^–6gmL^–1) was 3.8%. The proposed method has been successfully applied to the determination of sulfite in pharmaceutical injections and white sugar samples.     

A Novel Electrogenerated Chemiluminescence Reaction Scheme Using Core-Shell LuminoI-Based SiO2 Nanoparticles as a Regulator and Its Analytical Application

A novel core-shell luminol-based SiO2 nanoparticle While these nanoparticles were used as electrogenerated was synthesized by two step micro-emulsion method. chemiluminescence （ECL） reagent, the electrochemical （EC） reaction as well as the subsequent chemiluminescence （CL） reaction not only could be separated spatially, but also presented high efficiency for analytical purpose. In this case, the core-shell luminol-based SiO2 nanoparticles offered more potential to avoid the contradiction between the EC and the CL reaction conditions. A new ECL method based on the nanoparticle was developed, and isoniazid was selected as a model analyte to illustrate the characteristics of this new ECL method. Under the selected conditions, the proposed ECL response to isoniazid concentration was linear in the range of 1.0 ×10^-10 to 1.0 × 10^-6 g/mL with 2 × 10^-11g/mL detection limit.     

Highly Sensitive Electrogenerated Chemiluminescence Detecting Ranitidine Based On Chemically Modifying Microenvironment of the Chemiluminescence Reaction

Using a graphite electrode modified with vaseline and NiO, ranitidine showed a strongly ECL enhancing effect for the weak ECL signal of electrooxidation of luminol. Based on this finding, a more sensitive ECL method for ranitidine was firstly proposed. Under the optimum experimental conditions, the ranitidine hydrochloride concentration in the range of 3.0×10^?8–9.0×10^?6 mol/L was proportional to the enhancing ECL signal and offered a 9×10^?9 mol/L detection limit for ranitidine hydrochloride. At the same time, based on the investigation on this ECL reaction mechanism, a new concept, to improve the suitable ECL reaction micro‐environment with chemically modified electrode technique for the better analytical performances of ECL analysis was also firstly proposed.     

A Simple,Cost‐effective,and Environmentally Friendly Method for Determination of Ciprofloxacin in Drugs and Urine Samples Based on Electrogenerated Chemiluminescence

Ciprofloxacin is an antibiotic that belongs to the class of drugs known as quinolones and it is frequently used to treat a variety of bacterial infections. The present work aims the development of a simple, cost‐effective, and environmentally friendly method for the determination of ciprofloxacin in drugs and artificial urine samples due to the high importance of this antibiotic for the human health. The proposed method is based on the electrogenerated chemiluminescence (ECL) resulting from the reaction between the ciprofloxacin and the tris(2,2′‐bipyridyl)ruthenium(II) complex. This method exploits a screen‐printed carbon electrode positioned in an ECL cell with capacity to 50 μL of electrolytic solution. The ECL intensity was monitored with the aid of a photodiode. The ECL signal was simultaneously registered to the voltammetric measurements. Under optimized experimental conditions, the ECL method presented a linear response range for ciprofloxacin between 0.5 and 500 μmol L^?1 (or 0.0005 and 0.5 mmol L^?1). The proposed method presented a detection limit of 0.5 μmol L^?1 and it was successfully applied for the ciprofloxacin determination in drugs and artificial urine samples, with good accuracy and precision.     

Flow-Injection Electrochemiluminescence Detecting Rifampicin Based on Its Sensitizing Effect

Introduction Rifampicin, a semi synthetic compound, is used primarily in the treatment of tuberculosis, and it is also an excellent amistaphyloco antibiotic when used in combination with other antibiotics, so it is very useful clinically.1 Therefore, the determination of rifampicin has attracted much attention. From now on, techniques for the determination of rifampicin have been primarily based on spectrophotometry,2 double-wavelength thin-layer scan,3 chemiluminescence,4 high performance liq…     

采用甲基丙烯酸丁酯整体微柱芯片系统测定血样中痕量异烟肼

将合成的甲基丙烯酸丁酯(BMA)整体柱与微流控芯片技术结合,在PMMA芯片上以K3Fe(CN)6-NaOH-异烟肼化学发光体系为样品对象,在优化混合发光试剂比例和流速以及选择适合的洗脱液基础之上,实现了BMA整体微柱对异烟肼样品的富集作用,平均富集倍数和回收率分别达到16.8和84.2%,由此建立了流动注射化学发光(FIA-CL)芯片系统测定血液中痕量异烟肼的浓度的方法,可有效地实现异烟肼血药浓度分析的片上预处理和快速测定,检出限低于0.2 mg/L。     

Enhanced Electrochemical Response of Isoniazid Electrocatalytic Oxidation on RGO-PPD-SiW Modified Electrode

The electroactive composites based on reduced graphene oxide (RGO), poly-o-phenylenediamine (PPD) and heteropolyacids – H₄SiW₁₂O₄₀nH₂O (SiW) and H₃[PW₁₂O₄₀] ⋅ nH₂O (PW) was applied to a screen-printed carbon electrode (SPCE) as a planar three-electrode cell as the first step to creating various devices, in particular, sensors and catalysts. We studied potential use of the modified and unmodified SPCE planar electrode in determining the concentration of antitubercular antibiotic isoniazid (isonicotinic acid hydrazide C₆H₇N₃O or INH). The best result was observed for SPCE+RGO-PPD-SiW. CV of normal saline with various concentrations of C₆H₇N₃O demonstrated linear dependence of the relevant anodic peak current either in the bulk solution upon immersion of the modified electrode or in a droplet on the electrode surface.