共查询到20条相似文献，搜索用时 15 毫秒
1.
《Journal of Raman spectroscopy : JRS》2017,48(1):22-29
Tracing aetiology and pathogenesis of urinary stone disease is of great importance in order to prescribe appropriate treatment and prevent recurrences. For this purpose, morphological examination combined with determination of chemical composition of urinary stones is fundamental. In this work, we have evaluated the potential of multimodal nonlinear optical imaging for investigation of (micro)structure and chemical composition of human urinary stones. The method provides high‐resolution multimodal images of the cross‐sectioned stones without any labelling or some other pretreatment of the samples. We have shown that various constituents of urinary stones can be well discriminated in the multimodal images according to their optical signals. In addition, small structures (1–5 µm in size) were observed in the cross‐sections of urinary stones of various types. These structures were identified as crystallites of uric acid. They could either act as an active element during formation of urinary stones or, more probably, be accidentally incorporated into their structure, as it is suggested by random distribution of the crystallites. The results of this work show that multimodal nonlinear optical imaging can provide relevant information about growth processes of urinary stones and deliver useful insights in aetiology and pathogenesis of urolithiasis. Copyright © 2016 John Wiley & Sons, Ltd. 相似文献
2.
Vishnu Vardhan Krishnamachari Eric Olaf Potma 《Journal of Raman spectroscopy : JRS》2008,39(5):593-598
Focus‐engineered coherent anti‐Stokes Raman scattering (FE‐CARS) microscopy is used to highlight the lateral interfaces between chemically distinct media. Interface highlighting is achieved by using a HG10 mode for the Stokes laser beam and a HG00 mode for the pump laser beam in the forward detection scheme. The spectral and the orientation dependence of FE‐CARS are found to be in agreement with theoretical predictions. A brief discussion on the relevance of this technique for imaging third‐order nonlinear susceptibility interfaces in thin samples of biological or chemical importance is presented. Copyright © 2008 John Wiley & Sons, Ltd. 相似文献
3.
Rabah Mouras G. Rischitor A. Downes D. Salter A. Elfick 《Journal of Raman spectroscopy : JRS》2010,41(8):848-852
A multimodal nonlinear optical microscope that combines coherent anti‐Stokes Raman scattering (CARS), two‐photon excitation fluorescence (TPEF), second‐harmonic generation (SHG) and sum‐frequency generation (SFG) was developed and applied to image breast cancer tissue and MCF‐7 cells as well as monitoring anticancer drug delivery in live cells. TPEF imaging showed that drugs are preferentially localized in the cytoplasm and the nuclear envelope in resistant cells. Moreover, the extracellular matrix was observed by TPEF signals arising from elastin's autofluorescence and SHG signals from collagen fibrils in breast tissue sections. Additionally, CARS signals arising from proteins and (PO2)− allowed identification of tumors. Label‐free imaging with chemical contrast of significant components of cancer cells and tissue suggests the potential of multimodal nonlinear optical microscopy for early detection and diagnosis of cancer. Copyright © 2010 John Wiley & Sons, Ltd. 相似文献
4.
Madeleine keson Christian Brackmann Lena Gustafsson Annika Enejder 《Journal of Raman spectroscopy : JRS》2010,41(12):1638-1644
Glucose is one of the most fundamental molecules within life and bioengineering sciences. Present understanding of its role in cellular and bioengineering processes relies primarily on invasive, large‐scale biochemical analysis, providing no spatial information on glucose pools or fluxes. This work identifies an emerging microscopy technique based on coherent anti‐Stokes Raman scattering (CARS), which fulfills the need of quantitative imaging of glucose at the single‐cell level with submicrometer resolution. No sample preparation with reporter molecules is required, ensuring that the low‐weight metabolite is studied under natural conditions. The potential of CARS microscopy is illustrated by quantitatively mapping glucose fluxes and distributions in a microfluidic bioreactor and in lipid‐bilayer vesicles, the latter as a model for glucose transmembrane transport. Furthermore, the metabolic response to a glucose pulse was monitored in living yeast cells. This study signifies a new era within CARS microscopy for its use of monitoring carbohydrates, in particular glucose which is one of the most abundant molecules in nature. Copyright © 2010 John Wiley & Sons, Ltd. 相似文献
5.
Israel Rocha‐Mendoza Paola Borri Wolfgang Langbein 《Journal of Raman spectroscopy : JRS》2013,44(2):255-261
We demonstrate a technique for simultaneous detection of coherent anti‐Stokes Raman scattering (CARS) at four vibrational frequencies, using simple passive optical elements and without spectrally resolved detection. The technique is based on pump and Stokes femtosecond pulses selectively exciting vibrational resonances through spectral focusing. By replicating the pump and Stokes pair into four pairs, each traveling through appropriate glass elements, we simultaneously excite four different vibrational frequencies. The resulting CARS is a periodic train of intensities detected by a single photomultiplier and frequency analyzed to retrieve its Fourier coefficients. We demonstrate detection of methanol and ethanol mixtures in water and quantitative determination of their concentration owing to the improved chemical selectivity of this quadruplex CARS scheme. Copyright © 2012 John Wiley & Sons, Ltd. 相似文献
6.
超分辨成像及超分辨关联显微技术研究进展 总被引：1，自引：0，他引：1
光学成像系统中有限孔径对光波的衍射,使得光学显微成像技术的分辨率受到\"衍射极限\"限制而无法进一步提高.自1873年E.K.Abbe提出该问题以来,衍射极限就一直是学术界研究的热点.近年来,随着高强度激光、高灵敏探测器等光电器件研制技术以及新型荧光探针开发等相关领域的快速发展,光学显微技术衍射极限问题的研究迎来了新的契机,超分辨显微成像技术(super-resolution microscopy.SRM)在近十年内取得了令人瞩目的巨大成就.本文从空域和频域角度回顾了衍射极限分辨率的基本原理,并据此对目前常见的各种SRM技术\"绕过\"衍射极限提高分辨率的机理给予了详解,同时介绍了各类技术的发展动态和研究方向;作为SRM的一个新的重要的发展趋势,本文详细介绍了超分辨关联显微技术的最新研究进展,包括SRM与活细胞实时荧光显微、荧光寿命显微、光谱测量和成像、电子显微、原子力显微、质谱技术等的关联,着重讨论了各类超分辨关联显微技术的作用和意义;最后,对SRM技术和超分辨关联显微技术的未来发展方向进行了展望. 相似文献
7.
Kentaro Furusawa Norihiko Hayazawa Satoshi Kawata 《Journal of Raman spectroscopy : JRS》2010,41(8):840-847
A two‐beam multiplexed coherent anti‐Stokes Raman scattering (CARS) microscopy setup is demonstrated by using a broadband (BB) Ti:sapphire oscillator without using any specialty fibres. A well‐defined spectral structure of the source leads to a delay‐sensitive CARS measurement in two‐colour CARS and also provides an efficient means of obtaining three‐colour CARS signals combined with the dispersion compensation of the BB pulse. Our result implies that the background suppression is limited by the onset of the spurious signals caused by the different CARS process, qualitatively differing from what is typically observed in the CARS microscopy. Copyright © 2010 John Wiley & Sons, Ltd. 相似文献
8.
电子器件散粒噪声测试方法研究 总被引：3，自引：0，他引：3
本文分析了超导量子干涉器(SQUID)和超导-绝缘-超导(SIS)约瑟夫森结散粒噪声测试方法的应用局限性,提出了常规器件的散粒噪声测试方案.针对常规电子器件散粒噪声特性,研究了噪声测试基本条件,并建立了低温测试系统.通过采用双层屏蔽结构和超低噪声前置放大器,实现了较好的电磁干扰屏蔽和极低的背景噪声.在10 K温度下对常规二极管散粒噪声进行了测试,通过理论和测试结果对比分析,验证了测试系统的准确和可信性.关键词：散粒噪声电子器件噪声测试 相似文献
9.
Denis Akimov Susana Chatzipapadopoulos Tobias Meyer Nicolae Tarcea Benjamin Dietzek Michael Schmitt Jürgen Popp 《Journal of Raman spectroscopy : JRS》2009,40(8):941-947
We demonstrate that coherent anti‐Stokes Raman scattering (CARS) microscopy can be used not to obtain vibrational contrast related to a particular chemical species only, but furthermore, it directly yields topological contrast. Topological contrast is due to the spatially dependent refractive index of the sample and a number of linear effects as interference, refraction and absorption contribute to the topological contrast inherent to CARS images. In order to distinguish between different contrast mechanisms we propose an image correction method based on the analysis of the nonresonant four‐wave mixing (FWM) signal distribution. Copyright © 2009 John Wiley & Sons, Ltd. 相似文献
10.
11.
B. Serrano J. Baselga J. Bravo F. Mikes L. Sese I. Esteban I. F. Piérola 《Journal of fluorescence》2000,10(2):135-135
Blends of poly(vinylacetate) (PVAc) and poly(cyclohexylmethacrylate) (PCHMA) labeled by copolymerization with 4-methacryloylamine-4-nitrostilbene (Sb), with (1-pyrenylmethyl)methacrylate (Py), or with 3-(methacryloylamine)propyl-N-carbazole (Cbz) were prepared by casting dilute solutions in tetrahydrofurane (THF) or chloroform. Films about 10 m thick were formed. Phase separation in two types of domains is observed by transmission optical microscopy (TOM) and epifluorescence microscopy (EFM): small craters of 1 to 10 m placed at the polymer-air interface and larger domains, on the scale of 100 m. The morphology of samples depends on the composition of the polymer blend and on solvent. The green fluorescence of Sb, the violet of Py, or the blue of Cbz provides imaging of the distribution of PCHMA in the different domains and in the matrix. It is thus observed that (i) superficial craters and large domains are formed mainly by PCHMA and (ii) the matrix is composed of PVAc in films cast from THF and it is a blend of the two polymers, homogeneous at the submicrometric scale, for chloroform. The emission intensity of Py, recorded by microfluorescence spectroscopy (MFS), yields a mapping similar to imaging detection. It is remarkable that in films cast from chloroform, the smaller domains are distributed with a 2D hexatic order disrupted by dislocations and disclinations, whereas in films cast from THF, a larger heterogeneity is found, denoting different mechanisms of solvent evaporation. 相似文献
12.
13.
Yuki Oka Masaki Yoshida Ayumi Minoda Philippe Leproux Makoto M. Watanabe Hideaki Kano 《Journal of Raman spectroscopy : JRS》2021,52(12):2572-2580
Living microalga Cyanidium caldarium was visualized using ultra-multiplex coherent anti-Stokes Raman scattering (CARS) microspectroscopy. The Raman band related to the SO42− ion at 982 cm−1 was detected at the peripheral part of the cells and showed a signal amplitude that was approximately threefold larger than that in the medium, indicating accumulation of SO42− ions. Depending on the incubation conditions, a broad and sharp Raman band at approximately 505 cm−1 was observed at the peripheral part and inside the cells, respectively. Based on comparison of the results with model substances, these broad and sharp bands were assigned to polysulfides and glycogen, respectively. 相似文献
14.
15.
Bernhard von Vacano Lars Meyer Marcus Motzkus 《Journal of Raman spectroscopy : JRS》2007,38(7):916-926
Broadband multiplex coherent anti‐Stokes Raman scattering (MCARS) microscopy allows the rapid chemical mapping and molecular imaging of untreated material samples with three‐dimensional sectioning capabilities. It can be realized with a single laser in a simple and robust setup using supercontinuum generation in a microstructured fiber. The successful implementation of a MCARS microscope is discussed in detail, its parameters are characterized, and applications are shown for the identification and mapping of polymer blends. An evolutionary fitting routine is presented, which allows a fully quantitative analysis of the MCARS information resulting in high‐contrast chemical maps. The established setup enables reliable day‐to‐day operation as a valuable tool for rapid material characterization. Copyright © 2007 John Wiley & Sons, Ltd. 相似文献
16.
目前研究准弹道输运纳米MOSFET散粒噪声的抑制时, 采取了完全不考虑其抑制, 或只强调抑制的存在而并未给出抑制公式的方式进行研究. 本文基于Navid模型推导了准弹道输运纳米MOSFET散粒噪声, 并得到了其在费米作用、库仑作用和二者共同作用三种情形下的抑制因子. 在此基础上, 对各抑制因子随源漏电压、栅极电压、温度及源漏掺杂浓度的变化特性进行了研究. 两者共同作用的抑制因子随源漏电压和栅极电压变化特性与文献中给出的实验结论相符合, 从而对实验上得到两者共同作用下的抑制因子随源漏电压和栅极电压的变化特性给出了理论解释. 相似文献
17.
The coherent anti‐Stokes Raman spectroscopy (CARS) technique is often used in the study of turbulent flames. Fast and accurate algorithms are needed for fitting CARS spectra for temperature and multiple chemical species. This paper describes the development of such an algorithm. The algorithm employs sparse libraries whose size grows more slowly with number of species than a regular library. It was demonstrated by fitting synthetic ‘experimental’ dual‐pump CARS spectra containing four resonant species (N2, O2, H2 and CO2), both with added noise and without it, and by fitting experimental spectra from a H2 air flat flame produced by a Hencken burner. In the four‐species example, the library was nearly an order of magnitude smaller than the equivalent regular library (fitting times are correspondingly faster), and the fitting errors in the absence of added noise were negligible compared to the random errors associated with fitting noisy spectra. When fitting noisy spectra, weighted least squares fitting to signal intensity, as opposed to least squares fitting or least squares fitting to square root of intensity, minimized random and bias errors in fit parameters. Copyright © 2011 John Wiley & Sons, Ltd. 相似文献
18.
Andrew D. Cutler Emanuela C. A. Gallo Luca M. L. Cantu 《Journal of Raman spectroscopy : JRS》2016,47(4):416-424
WIDECARS measures temperature and mole fractions of most of the major species in ethylene–air flames. One of the issues in implementing this technique is fitting the experimental spectra to theory to obtain flame conditions (temperature, species mole fractions). Individual spectra contain many species resonances, and theory is slow to compute. Libraries of precalculated spectra can be used, but a library of sufficient density for accurate interpolation is large given the many variables. A new fitting algorithm is presented which utilizes a less‐dense library, and additional spectra are calculated during fitting to maintain accuracy. The iterative convergence method converts the problem of minimizing fit error, which converges slowly, to a zero finding problem, which converges reliably, rapidly, and accurately to best fit. Various practical fitting issues, such as the effects of dye laser mode noise and variability, phase‐matching efficiency, and shifts of the spectrum on the spectrometer are addressed. The technique is demonstrated in the analysis of experimental measurements in an equivalence ratio 2.1 ethylene–air flame above the surface of a McKenna burner. Precision errors because of experimental and fitting effects are discussed. Copyright © 2015 John Wiley & Sons, Ltd. 相似文献
19.
为了准确、客观地评价可见光光电成像系统整机性能参数,研制了可见光光电成像系统整机综合参数校准装置。装置由积分球光源系统、标准测试卡、光学准直系统、支撑调整台、视频数据采集模块和综合处理软件等几部分组成,主要完成系统分辨力、调制传递函数（MTF）、对比度、噪声功率谱、噪声等效亮度、灵敏度等性能参数的测量。针对某型号可见光电视产品,验证了装置的有效性,给出了综合参数的测量结果,其截止频率为39.4 mm-1,对比度为76.5％,均方根噪声电压为2.14 mV,噪声等效亮度(NEL)为0.045 5 cd/m2,信号传递函数对应的灵敏度为47 mV（cd/m2)-1。最后,分别采用照度计和标准视频发生器对装置进行了标定,其积分球光源出口亮度和信号采集系统输出电压最大相对测量误差分别为-2.3％及1％。 相似文献