共振光散射法测定复方氨基酸注射液中色氨酸的含量

在pH1.98时,色氨酸和氯金酸发生氧化还原反应生成金纳米粒子。用扫描电镜、紫外-可见吸收光谱和荧光光谱对所生成的金纳米粒子进行了表征,并通过在普通荧光光度计上测定所生成的金纳米粒子的光散射信号,建立了定量检测色氨酸含量的方法。方法的线性范围为0.21～60.0μmol/L;检出限为0.02μmol/L。甘氨酸等10余种无还原性的氨基酸对体系不产生干扰。将所建立的方法用于复方氨基酸注射液中色胺酸的测定,相对误差小于5.7%;回收率在96.3%～104.6%之间。     

石墨烯/铂纳米粒子杂化膜测定肾上腺素

利用循环伏安法制备了石墨烯/铂纳米粒子杂化膜修饰电极,并利用该修饰电极研究了肾上腺素（EP）的电化学行为,建立了测定肾上腺素的电化学方法。 分别利用扫描电子显微镜（SEM）和循环伏安法对电极表面的形貌和电化学性能进行了表征。 试验优化了修饰电极制备过程中影响电极性能的条件和EP的测定条件。 试验结果表明,石墨烯/铂纳米粒子修饰电极对肾上腺素有明显的电催化作用。 在pH=5.0的柠檬酸 磷酸氢二钠缓冲溶液中,EP的氧化峰电流与其浓度在4.4×10-8~2.2×10-6 mol/L的范围内呈良好的线性关系。 线性方程为ipa(10 μA)=0.0753c(mol/L)+3.7653×10-5,r=0.9989,检出限为2.2×10-9 mol/L（S/N=3）。 修饰电极表具有良好的重现性,可用于实际样品的测定。     

银包金纳米棒的形成及维生素C的局域等离子体共振吸收位移测定法

本文以金纳米棒作为信号感应载体,在pH9.4弱碱性条件下通过维生素C还原AgNO3从而在金纳米棒表面形成包裹层,进而改变金纳米棒的等离子体共振吸收位移,据此建立了一种快速、简单、灵敏的维生素C的等离子体共振吸收位移测定法.方法的线性范围为0.25～10.0μmol/L,检出限(3σ)为0.12mol/L.该方法成功用于维生素C片剂分析,加标回收率95.4%～106.0%.与药典方法进行对比,有满意的精密度和准确度.     

纳米金-二氧化钛纳米针修饰电极用于氯霉素检测

将金胶-TiO2纳米针修饰玻碳(Au-TiO2/GC)电极用于对氯霉素(CAP)浓度的电化学测定。 结果表明,在0.1 mol/L pH=7.0的磷酸盐缓冲溶液(PBS)体系中,CAP在Au-TiO2/GC电极上有较好的电化学响应,CAP浓度在8.0×10-7～1.0×10-4 mol/L范围内,峰电流与其浓度呈良好的线性关系,检出限为7.8×10-8 mol/L。 并对氯霉素滴眼液中CAP浓度进行了检测,发现该方法有高的检测灵敏度、好的重复性和抗干扰性,且操作简便、成本低廉。     

基于发光金纳米粒子荧光增强法测定溶菌酶

参照文献方法合成了BSA修饰的水溶性发光金纳米粒子,并考察了其与溶菌酶之间的相互作用.依据溶菌酶对金纳米粒子的发光增强现象,建立了测定溶菌酶的荧光新方法.考察了发光金纳米粒子的浓度、pH值、反应时间及共存物质对测定的影响.优化条件为:发光金纳米粒子浓度4.0×10(-6)mol/L,pH 7.0、反应时间10 min....     

基于金纳米粒子自组装的分光光度法测定半胱氨酸

在pH 4.56的B ritton-Rob inson(B-R)缓冲溶液中,半胱氨酸的SH和NH3 分别与金纳米粒子表面进行共价结合和静电作用,导致金纳米粒子的长距离自组装,形成网状超分子结构,并使金纳米粒子的最大吸收波长从520 nm红移到660 nm。本实验对半胱氨酸引导的金纳米粒子自组装的作用机制进行了研究,建立了操作简便、高灵敏度测定半胱氨酸的分析方法。其线性范围为0.01~0.20 mg/L;检出限为2.8μg/L(3,σ2.3×10-8mol/L)。在实验条件下,其它常见的氨基酸和谷胱甘肽均不干扰测定。     

金纳米颗粒等离子体共振吸收光谱检测头孢唑啉

根据金纳米粒子等离子体共振吸收引起的溶液颜色变化,建立了一种快速、简便检测头孢唑啉的色度分析方法.方法线性范围为0.1～5.0 μmol/L,检测限为14 nmol/L.将该方法用于头孢唑啉钠粉针剂的分析,回收率在97.4％～100.2％之间,相对标准偏差小于6.1％.     

纳米金比色法测定阿米卡星

阿米卡星诱导金纳米粒子的快速团聚,使其在520 nm处吸收值降低,同时在640 nm附近产生新吸收,且两处吸光度比值(A640/A520)的变化与阿米卡星的浓度呈正比,据此建立了测定阿米卡星的新方法。对p H、反应时间以及纳米金浓度等实验条件进行了优化,在优化实验条件下,测定阿米卡星的线性范围为2.0×10-7~5.0×10-6mol/L,检出限为0.6×10-8mol/L,相对标准偏差(RSD)为1.9%(n=11)。方法用于硫酸阿米卡星注射液的测定,回收率在100.0%~105.0%。     

直接电沉积金纳米粒子修饰氧化铟锡电极测定亚硝酸根

以电化学沉积法一步制得了金纳米粒子(GNP)修饰氧化铟锡(ITO)电极,采用紫外、扫描电镜及循环伏安法对GNP/ITO修饰电极进行了表征。结果表明,金纳米粒子在ITO电极表面呈球形,分布均匀无团聚,粒径约30 nm。该修饰电极具有良好的电化学性能,在pH 2.2的Na2HPO4-柠檬酸缓冲溶液中其氧化峰电流与NO2-的浓度呈良好的线性关系,线性范围为5×10-6~5.5×10-4mol/L,线性回归方程为:i(μA)=1.07 136C(mmol/L),相关系数r=0.9969;检出限可达1.0×10-6mol/L。该修饰电极用于废水中NO2-的测定,结果令人满意。     

银纳米粒子表面等离子共振吸收-分光光度法测定废水中的邻苯三酚

基于改良银镜反应,利用银纳米粒子的表面等离子共振吸收提出了测定废水中邻苯三酚的分光光度法。室温条件下,在氢氧化钠和氨水混合溶液的碱性环境中,以吐温-20作为分散剂,邻苯三酚作为还原剂,还原银氨溶液,反应生成银纳米粒子,溶液由无色变为亮黄色,并在410nm处产生银纳米粒子表面等离子共振吸收峰。溶液的吸光度与邻苯三酚的浓度在3.96×10-7~2.38×10~(-5) mol·L~(-1)范围内呈线性关系,检出限(3σ)为2.97×10-7 mol·L~(-1),摩尔吸光率为5.483×104L·mol~(-1)·cm~(-1)。该方法应用于工业废水中邻苯三酚的测定,测定值与普通分光光度法的测定结果一致,相对标准偏差(n=5)小于5.0%,加标回收率在95.4%~104%之间。     

银纳米粒子探针等离子共振散射光谱法测定抗坏血酸

利用抗坏血酸(VC)还原银氨溶液生成具有强烈等离子体共振散射特性的银纳米粒子,导致体系共振光散射信号的增强,散射最大峰位于544 nm。 在最佳条件下,体系的共振散射强度与抗坏血酸浓度在0.2~7.0 μmol/L范围内呈线性关系,相关系数为0.9988,检出限为8.1 nmol/L。 抗坏血酸的氧化产物能与银纳米粒子相互作用使之分散均匀且保持稳定,因此该体系不需要添加稳定剂或分散剂。 以此建立了用银纳米粒子为探针的测定抗坏血酸的高灵敏、简捷的共振散射新方法。 同时讨论了最佳反应条件和其它影响因素。 该方法用于ＶＣ片及饮料中抗坏血酸的测定,加标回收率在94.5%~97.0%之间。 结果表明,方法具有操作简单,灵敏度高和回收率较好等优点。     

Fluorescence quenching method for the determination of catechol with gold nanoparticles and tyrosinase hybrid system

<正>The determination method of catechol by fluorescence quenching was developed.The assay was based on the combination of the unique property of gold nanoparticles with tyrosinase enzymatic reaction.In the presence of tyrosinase,the fluorescence of gold nanoparticles was quenched by catechol which can be employed to detect catechol.Under the optimal conditions,a linear range 5.0×10~(-7)-1.0×10~(-3) mol L~(-1) and a detection limit 1.0×10~(-7) mol L~(-1) of catechol were obtained.o-Quinone intermediate produced from the enzymatic catalyzed oxidation of catechol was considered to play the main role in the fluorescence quenching.     

应用纳米磁性球电化学检测特定序列DNA

采用分散聚合法制备纳米磁性羧基球,利用化学偶联法将末端修饰氨基的寡聚核苷酸固定在纳米磁性球表面,制成新型核酸探针,该探针可特异性结合目标单链寡聚核苷酸.在磁场作用下,将纳米磁珠与本体溶液分离并富集在电极表面,以中性红为嵌合指示剂,用示差脉冲伏安法测定杂交结果.经过条件优化,本法测定DNA的浓度线性范围为1.0×10^-6～5.0×10^-9mol/L,检出限为8.6×10^-10mol/L.     

Bi(Ⅲ)-茜素红-CTMAB体系共振散射光谱法测定环境水样中痕量铋

在pH=4.1的HCl溶液中,Bi(Ⅲ)与茜素红和十六烷基三甲基溴化铵（CTMAB）缔合形成粒径较大的疏水性三元离子缔合物（结合比为n(Bi(Ⅲ))∶n(AR)∶n(CTMAB)=1∶3∶3）,该反应导致共振光散射显著增强,最大散射峰位于364 nm。 研究了反应介质、pH值、共振探针浓度等对散射体系的影响,考察了该散射反应的稳定性及共存物质的影响,并对该三元离子缔合物的反应机理进行了探讨。 在2.5 mL 1.0×10-4 mol/L茜素红和2.0 mL 1.0×10-4 mol/L CTMAB最优试验条件下,Bi(Ⅲ)质量浓度与共振光散射强度ΔI364 nm在0～0.384 mg/L范围内呈良好线性关系,检测限为5.898×10-8 g/L,对含量为0.18 mg/L Bi(Ⅲ)溶液进行11次平行测定,其相对标准偏差为2.2%。 将该方法用于环境水样中痕量铋(质量浓度为0.552～0.831 μg/L)的测定,加标回收率为99.5%～100.2%,测定偏差小于2.7%。 基于Bi(Ⅲ)-AR-CTMAB三元离子缔合物的共振光散射光谱,建立了以茜素红染料为光谱探针的痕量Bi(Ⅲ)共振光散射检测新方法。     

Self-assembly of lysozyme on the surfaces of gold nanoparticles

The interaction of lysozyme(Lys) and gold nanoparticles was investigated via UV-vis absorption and resonance light-scattering method.There are some changes of the plasmon absorption and resonance light-scattering of gold nanoparticles that were observed via the addition of Lys.The normalized plasmon absorption and resonance light-scattering intensity with gold nanoparticles were both linear wilh 1-20 nmol/L Lys.A simple model about the component of the gold nanoparticles and Lys complex was established and the calculated result was fitted well in their concentration ratio.Furthermore,the activity analysis of Lys showed that the interaction was weak and nondestructive.     

金纳米粒子-荧光素体系的光谱特性

纳米粒子具有量子尺寸效应和表面效应等许多特有的性质 [1] ,在光吸收、医药及新材料等方面具有广阔的应用前景 .纳米粒子具有较高的比表面能且带有电荷 ,当光子与其接近时 ,实际上是光子与纳米粒子的界面电子发生了作用 [2 ,3 ] .基于此建立的共振散射 (RS)光谱技术已成为一种高灵敏度和高选择性的分析技术 ,是研究生物化学和液相纳米粒子特性的良好手段 [4～ 9] .我们 [2 ,3 ] 研究发现 ,较大粒径纳米粒子和界面的形成是导致散射光增强的根本原因 ;金、银等液相纳米粒子产生 RS效应和 RS峰等 .荧光猝灭 (FQ)效应已用于分析化学和蛋白…     

A simple and sensitive assay of gallic acid based on localized surface plasmon resonance light scattering of silver nanoparticles through modified Tollens process

A simple, low toxic, sensitive strategy based on the localized surface plasmon resonance light scattering (LSPR-LS) properties of silver nanoparticles (AgNPs) is introduced for the detection of gallic acid (GA). It was found that the silver ammonium complex, [Ag(NH(3))(2)](+)(aq), could be reduced in the alkaline medium by GA at room temperature; this reaction formed dispersed AgNPs. Transmission electron microscopy analyses were performed to ascertain the formation of AgNPs. UV-visible spectra revealed the localized surface plasmon resonance (LSPR) absorption at 410 nm corresponding to the LSPR of AgNPs. On these basis, we could quantify the GA concentration in the range of 4 × 10(-7) - 5 × 10(-6) mol L(-1) in the optimized experimental conditions. This method was used for determining the concentration of GA in artificial samples with satisfactory results. The detailed mechanism underlying this special phenomenon was elucidated.     

纳米金共振散光谱探针测定钼

在0．45mol／L硫酸-0．026mol／L硫脲-0．32mol／LKSCN介质中,粒径为70nm的纳米金的共振散射信号较弱,Mo（Ⅵ）被硫脲还原为Mo（Ⅴ）,Mo（Ⅴ）与硫氰酸钾生成橙红色配合物[MoO（SCN）s]^2-．该配合物与纳米金探针作用,导致402和554nm共振散射峰增强．钼浓度在1．O～20×10^-6mol／L范围内与402nm波长的共振散射光强度成线性关系,方法的检出限为5．1×10^-7 mol／L Mo．该法用于废水中钼的测定,结果满意．     

Resonance light-scattering spectrometric study of interaction between enzyme and MPA-modified CdTe nanoparticles

This paper described a novel assay of enzyme based on the measurement of enhanced resonance light-scattering (RLS) signals resulting from the electrostatic and coordination interaction of functionalized CdTe nanoparticles with enzyme. The CdTe nanoparticles which were modified with 3-mercaptocarboxylic acid (MPA) have abundant carboxylic groups (COOH). So the nanoparticles are water-soluble, stable and biocompatible. At pH 8.3 phosphate buffered saline (PBS), the RLS signals of functionalized nano-CdTe are greatly enhanced by bromelain and papain in the region of 220-800 nm characterized by the peak around 318-314 nm, respectively. The optimization conditions of the reaction were also examined and selected. Under the selected conditions, the enhanced RLS intensity is linearly proportional to the concentration of bromelain and papain. The liner range is (0.09-0.9) x 10(-6)mol/L for bromelain and (0.048-0.702) x 10(-6)mol/L for papain. The influences of some foreign substances were also examined. This method can be applied to the determination of enzyme.     

阿莫西林与维多利亚蓝B的显色反应及应用

The color reaction of amoxicillin with victoria blue B was studied.A novel visible spectrophotometric method for the determination of amoxicillin has been established.Under pH 8.50～10.5 condition,victoria blue B reacteds with amoxicillin to form red ion association complex.The maximum absorption wavelength was at 630nm and the apparent molar absorptivity(ε) was 2.67×10~4 L·mol~(-1)·cm~(-1) and the linear ranges was 0～5.5 mg·L~(-1).Amoxicillin obey Beer's law in definite concentration range.The optimum reaction conditions and the methodological precision were studied simultaneity.The method was simple and rapid and sensitive for the determination of amoxicillin.This method has been applied to the determination of amoxicillin is in the city sell amoxicillin medicament is in point and average recovery at 98.2%～101.0%.