A new analysis of the depolymerized fragments of lignin polymer in the plant cell walls using ToF-SIMS

High mass resolution ToF-SIMS spectra by Au⁺ primary ion bombardment were used to investigate exact structures of characteristic ions of lignin in plant cell walls. Previous study using Ga primary ion bombardment showed the characteristic peaks of guaiacyl lignin at m/z 137 ([C₈H₉O₂]⁺) and 151 ([C₈H₇O₃]⁺ and [C₉H₁₁O₂]⁺), but it was unclear whether the peak at m/z 151 in the spectrum of lignin in situ in plant cell walls is actually a double-component, [C₈H₇O₃]⁺ (151.0394) and [C₉H₁₁O₂]⁺ (151.0758). This report achieved a higher mass resolution with lignin samples, showing that the peak at m/z 151 is dominated by the C₆-C₁ benzoyl ion, [C₈H₇O₃]⁺, not the C₆-C₂ ion, [C₉H₁₁O₂]⁺.     

The influence of the cholesterol microenvironment in tissue sections on molecular ionization efficiencies and distributions in ToF-SIMS

High-resolution images of cholesterol were obtained from Lymnaea stagnalis nervous tissue using metal-assisted (MetA) time-of-flight secondary ion mass spectrometry (ToF-SIMS). The spatial distributions of different pseudomolecular ions of cholesterol [M − H]⁺, [M − OH]⁺ and [2M + Au]⁺, illustrate the influence of the tissue microenvironment on the ionization efficiencies of these ions. These biological matrix effects result in differences in localizations of molecular ions derived from the same molecular species.     

Ga primary ion ToF-SIMS fragment pattern of inorganic compounds and metals

Regularity of Ga⁺ primary ion ToF-SIMS fragment pattern of inorganic compounds is discussed. For an inorganic compound as formulated M–A, where the valence of cation M is +n and that of anion A is −p, the chemical composition of appeared ToF-SIMS fragment are M_xA_y, which satisfy the rule nx≥py+1 for positive ion fragments and nx≤py+1 for negative ones. For example, for oxide fragment of chemical composition, M_xA_y (valence of M is +n), the fragment obeys the rule nx≥2y+1 for positive ions and nx≤2y+1 for negative ones, respectively. The regularity of ToF-SIMS fragment patterns of sulfides, nitrates, sulfates etc. is discussed.     

Investigating lipid interactions and the process of raft formation in cellular membranes using ToF-SIMS

There is an increased interest in how lipids interact with each other, especially in the lateral separation of lipids into coexisting liquid phases as this is believed to be an attribute of raft formation in cell membranes. ToF-SIMS has shown itself to be an excellent tool for investigating cellular and model membrane systems and will be perhaps the most powerful one for investigating raft formation. Results from our laboratory show the capability of ToF-SIMS at identifying unequivocally the content of coexisting liquid lipid phases. Using supported lipid monolayers we find that the inclusion of dipalmitoylphosphatidylethanolamine (DPPE) to a homogeneous dipalmitoyl-phosphatidylcholine (DPPC)/cholesterol phase results in the formation of cholesterol-rich domains [A.G. Sostarecz, C.M. McQuaw, A.G. Ewing, N. Winograd, J. Am. Chem. Soc. 126 (2004) 13882]. Also, for DPPE/cholesterol systems a single homogeneous DPPE/cholesterol phase is formed at ∼50 mol% cholesterol, whereas DPPC/cholesterol systems form a single phase at 30 mol% cholesterol [C.M. McQuaw, A. Sostarecz, L. Zheng, A.G. Ewing, N. Winograd, Langmuir 21 (2005) 807]. Currently we are exploring the incorporation of sphingomyelin into phospholipid-cholesterol mixtures in an effort to gain a better understanding of its role in raft formation.     

Laser activation-modification of semiconductor surfaces (LAMSS) of 1-alkenes on silicon: A ToF-SIMS, chemometrics, and AFM analysis

Laser-activation-modification of semiconductor surfaces (LAMSS) was carried out on silicon with a series of 1-alkenes. These laser spots were studied by time of flight secondary ion mass spectrometry (ToF-SIMS). The resulting spectra were analyzed using the multivariate curve resolution (MCR) method within the Automated eXpert Spectral Image Analysis (AXSIA) toolkit, and also by MCR and cluster analysis using commercially available toolboxes for Matlab: the PLS_Toolbox and the MIA_Toolbox, respectively. AXSIA based MCR generally finds three components for the spectral images: one for the background and two for the laser-activated spots, for both the positive and negative ion images. The negative ion component spectra from the spots show increased carbon and hydrogen signals compared to oxygen. They also show reduced chlorine and fluorine (contamination) peaks. In order to compare AXSIA-MCR results from different images, the AXSIA component spectra of different spots were further analyzed by principal components analysis (PCA). PCA of all of the negative ion components shows that component 1 is chemically distinct from components 2 and 3. PCA of all of the positive ion components yields the same result. The loadings plots of this PCA analysis confirm that component 1 generally contains fragments expected from the substrate, while components 2 and 3 contain fragments expected from an overlayer composed of alkyl chains in the spots. A comparison of the two MCR analyses suggests that roughly the same information can be obtained from AXSIA, which is not commercially available, and the PLS_Toolbox. Cluster analysis of the data also clearly separates the spots from the backgrounds. A key finding from these analyses is that the degree of surface functionalization in a LAMSS spot appears to decrease radially from the center of the spot. Finally, a comparison of atomic force microscopy (AFM) of the spots versus the AXSIA analysis of the ToF-SIMS data produced another important result, which is that the surface morphology is only weakly correlated with the LAMSS chemistry.     

超二代及三代像增强器不同响应波段的参数测量及比较

对A光源分别进行550 nm、625 nm以及675 nm的短波截止,对比测量了超二代和三代像增强器的光谱响应、阴极灵敏度、增益、分辨力以及信噪比.在10-1 lx照度条件下,当对A光源进行675 nm的短波截止之后,三代像增强器的分辨力未出现下降,而超二代像增强器的分辨力却下降到初始值的94％;但在10-4 lx照度...     

Scaling of x-ray emission and ion velocity in laser produced Cu plasmas

The x-ray emission from slab targets of copper irradiated by Nd:glass laser (1.054 μm, 5 and 15 ns) at intensities between 10¹² and 10¹¹W/cm² has been studied. The x-ray emissions were monitored with the help of high quantum efficiency x-ray silicon photo diodes and vacuum photo diodes, all covered with aluminium filters of different thickness. The x-ray intensity vs the laser intensity has a scaling factor of (1.2–1.92). The relative x-ray conversion efficiency follows an empirical relationship which is in close agreement with the one reported by Babonneau et al. The ion velocities were monitored using Langmuir probes placed at different angles and radial distances from the target position. The variation of the ion velocity with the laser intensity follows a scaling of the form Φ ^β where β ∼0.22 which is in good agreement with the reported scaling factor values. The results on the x-ray emission from Cu plasma are reported.     

Ground state energies of neutral and negatively charged donors in nanowire superlattice

Using the Bastard-type trial function for the neutral donor and the Hylleraas-type trial function for the negatively charged donor, we investigate the effect of donor positions inside the cylindrical GaAs/Ga(Al)As nanowire superlattice on their ground state energies. Results of calculation, presented in the form of contour plots of the energies corresponding to different donor positions along a cross section through symmetry axis, show a close relationship between the energies of the neutral and negatively charged donors and the charge distributions in one- and two-electron nanowire superlattices, respectively, at the point of their locations. The higher the charge density resulting from the unbound electron at a point in the heterostructure, the lower are the ground state energies of the neutral and negatively charged donors located at this point.     

Stark effect of negatively and positively charged excitons in semiconductor quantum wells

We study the effect of an electric field applied normal to the layers on the binding energy of charged excitons (or trions) in GaAs quantum wells. We find that, in contrast to the neutral exciton, their binding energy is sharply reduced by modest electric fields. The effect is stronger for the positively charged exciton than the negatively charged one. The ionisation of the excess carrier is explained by the field-induced polarisation of the electron and hole subband wave functions.     

微通道板近紫外量子效率测量及成像研究

提出了一种通过测量微通道板输出电流及增益来计算光电流,从而测量出微通道板量子效率的方法,并用该方法测量了微通道板在近紫外(200~380nm)的量子效率.测量结果表明,微通道板的量子效率很低,并且随波长增加而快速下降,200nm波长处的量子效率为10-4数量级,320nm波长处的量子效率为10-8数量级,大于340nm波长处的量子效率极低且趋近于零.微通道板及荧光屏组成的成像器件可以对酒精灯火焰成像,但图像较稀疏,而传统Cs2Te光电阴极紫外成像器件的图像却较密实,这与微通道板量子效率低,Cs2Te光电阴极量子效率高的情形一致.在该成像器件的前端放置一片350nm波长的高通滤光片后,所成的酒精灯火焰图像消失.对被照射目标成像时,如果照射光源为254nm的汞灯,则可以成像;但如果照射光源为365nm汞灯,则不能成像.说明微通道板的光谱响应主要在350nm波长以下,与其量子效率的测量结果一致.最后测量得到该成像器件的分辨力为32lp/mm,与传统Cs2Te光电阴极紫外成像器件的分辨力相同.微通道板及荧光屏组成的成像器件由于不使用光电阴极,具有价格低、寿命长且可靠性高的优点,因此可在紫外信号较强或成像距离较短的条件下使用.     

Thermal equilibrium concentrations and effects of negatively charged Ga vacancies in n-type GaAs

We have calculated the thermal equilibrium concentrations of the various negatively charged Ga vacancy species in GaAs. The triply-negatively-charged Ga vacancy, V _Ga ^3– , has been emphasized, since it dominates Ga self-diffusion and Ga-Al interdiffusion under intrinsic and n-doping conditions, as well as the diffusion of Si donor atoms occupying Ga sites. Under strong n-doping conditions, the thermal equilibrium V _Ga ^3– concentration, , has been found to exhibit a temperature independence or a negative temperature dependence, i.e., the value is either unchanged or increases as the temperature is lowered. This is quite contrary to the normal point defect behavior for which the point defect thermal equilibrium concentration decreases as the temperature is lowered. This property provides explanations to a number of outstanding experimental results, either requiring the interpretation that V _Ga ^3– has attained its thermal equilibrium concentration at the onset of each experiment, or requiring mechanisms involving point defect non-equilibrium phenomena.     

含有带正负电离子的等离子体中的非线性波研究

研究了含有带正负电的冷离子和热电子的磁化等离子体系统.运用约化摄动法从该系统的运动方程中推导出Zakharov-Kuznetsov(ZK)方程、改进的ZK方程和耦合ZK方程.给出了这些方程的一种孤立波解,得到了孤立波的振幅、宽度、传播速度与负离子和正离子的质量比、负离子数密度、磁场强度的关系以及正离子和负离子在运动过程中的位移图像. 关键词： Zakharov-Kuznetsov(ZK)方程 改进的ZK方程 耦合ZK方程 约化摄动法     

Quantification of Low Concentrations of DNA Using Single Molecule Detection and Velocity Measurement in a Microchannel

We present a novel method for quantifying low concentrations of DNA based on single molecule detection (SMD) for molecular counting and flow measurements inside a microchannel. A custom confocal fluorescence spectroscopic system is implemented to detect fluorescent bursts emitted from stained DNA molecules. Measurements are made one molecule at a time as they flow through a femtoliter-sized laser focal probe. Durations of single molecule fluorescent bursts, which are found to be strongly related to the molecular transit times through the detection region, are statistically analyzed to determine the in situ flow speed and subsequently the sample volume flowing through the focal probe. Therefore, the absolute concentration of a DNA sample can be quantified based on the single molecule fluorescent counts from the DNA molecules and the associated probe volume for a measured time course. To validate this method for quantifying low concentrations of biomolecules, we tested samples of pBR322 DNA ranging from 1 pM to 10 fM (∼3 ng/ml to 30 pg/ml). Besides molecular quantification, we also demonstrate this method to be a precise and non-invasive way for flow profiling within a microchannel.     

工业级纳米绒面多晶硅太阳电池的制备及其性能研究

基于产线工艺制备了纳米绒面多晶硅太阳电池,并表征其光电转换性能。研究结果表明：相对传统微米绒坑,纳米绒面能够提升多晶硅太阳电池的短路电流,相应的光电转换效率绝对值提升大于0.4%,产线均值光电转换效率超过了19.1%。结合漫反射光谱和外量子效率测试结果,改进的光电转换的原因归结为纳米绒面能够有效地诱捕短波和长波太阳光子,增强短波和长波太阳光响应。本研究证实纳米绒面多晶硅太阳电池可利用产线工艺制备且具有较高的光电转换效率,能够实现产业化。     

HPLC-DAD法测定辣椒及其制品中苏丹红的含量

建立了应用高效液相色谱测定辣椒粉及其少量含油制品中的苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ含量的检测方法.采用Symmetry C18反相色谱柱(3.9mm×150mm,5μm),流动相为乙腈醋酸溶液(V乙磷:Vo.1%醋酸＝7∶1),等度洗脱,流速1.0mL/min;柱温30℃;检测波长230nm.在选定色谱条件下,苏丹红Ⅰ、Ⅱ、Ⅲ、...     

Comparison of Mid-IR with NIR in Polymer Analysis

Abstract

Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) represents a powerful technique for analyzing biological samples due to the ultrahigh resolving power, high mass accuracy, and multiple-stage tandem mass spectrometry (MSⁿ). With the advent of electrospray ionization (ESI), determinations of binding stoichiometry and binding sites for protein complexes are available. This review summarizes the recent FT-ICR MS applications in characterization of protein complexes, such as protein-peptide complexes, protein-protein complexes, and protein-nucleic acid complexes. Especially, combined with ECD and SWIFT techniques, FT-ICR MS has unique ion manipulation capabilities and plays a critical role in the analysis of protein complexes.     

探测器对量子增强马赫-曾德尔干涉仪相位测量灵敏度的影响

研究了强度差测量方案下,探测器量子效率对光子数态、关联数态、压缩真空态三种量子光源注入的马赫-曾德尔干涉仪相位测量灵敏度的影响.获得了相位测量灵敏度与效率的定量关系,比较了探测效率对不同量子态注入的干涉仪相位灵敏度的影响.研究表明：光子数态注入时,相位测量灵敏度始终不能超越标准量子极限;关联数态注入时,无论多大的光子数,要获得相位测量的量子增强,探测效率不得小于75%;对于压缩真空态,只要有压缩存在就可以获得一定的相位测量的量子增强;关联数态、压缩真空态的注入,相位灵敏度皆随探测效率的增大而不同程度的提高,且压缩真空态比关联数态具有更好的量子增强效果.给出了在量子增强的精密测量实验中对探测效率的要求,并结合实际应用说明了探测效率的提高有助于提高干涉仪探测的灵敏度.     

Generation of UV laser light by stimulated Raman scattering in D2, D2/Ar and D2/He using a pulsed Nd:YAG laser at 355nm

A pulsed Nd:YAG laser at 355nm is used to pump Raman cell filled with D_2, D_2/Ar and D_2/He. With adequately adjusted parameters, the maximum photon conversion efficiency of the first-order Stokes light (S_1, 396.796nm) reaches 33.33% in D_2/Ar and the stability of S_1 in pure D_2 is fairly high, the energy drift being less than 10% when the pump energy drifts in the range of 5%. The conversion efficiency and stability, which are functions of the composition and pressure of the Raman medium and the energy of pump laser, are investigated. The result has been used to optimize the laser transmitter system for a differential absorption lidar system to measure NO_2 concentration profiles.     

A consistent parametrisation for the production rates of negatively charged hadrons and neutral strange particles in nucleon-nucleon,nucleon-nucleus and nucleus-nucleus collisions

A parametrisation of negative hadron and neutral strange particle production was developed which consistently describes presently available data from nucleon-nucleon, nucleon-nucleus and nucleus-nucleus collisions at a beam energy of 200 GeV per nucleon. Average multiplicities of negative hadrons are found to be proportional to the number of wounded nucleons, averageK _s ⁰ multiplicities proportional to the number of wounded quarks, and average and multiplicities proportional to the number of wounded quarks plus an additional contribution proportional to the number of interactions of secondary produced particles. Predictions are given for lead-lead collisions.On leave from Rudjer Boskovic Institute, Zagreb, Croatia     

The study of the target emissivity and detection using a superconducting detector in the submillimeter wave region

This paper researches mainly the radiation characteristics of the target and detecting techniques by superconducting detector in the submillimeter wave(SMMW) region. The calculation of transition wavelength(TW) between Planck and Rayleigh-Jeans is given out. The experiment of detecting different targets is finished by use of superconducting detector possessing high performances. Through the experiments in paper we can select and determine the wave bands of SMMW detecting and imaging system. The many novel theoretical analyses and experimental results are expressed in Figs and tables of the paper. Finally, a part of calculating results and experimental datum detected by a superconducting detector in SMMW regions are given in this paper.