微流控芯片荧光检测系统的研究进展北大核心CSCD

微流控芯片又称芯片实验室,具有检测高效、消耗试剂少、高通量、微型化和集成化等特点,许多检测方式(如光学检测、电化学检测)已经集成于微流控芯片上,而荧光检测是微流控芯片检测技术的常见手段之一。为此,在介绍了荧光检测技术的基本原理和光路结构的基础上,从激发光源、光传辅助手段和检测器等方面综述了微流控芯片荧光检测系统的研究进展,并对其发展进行了展望(引用文献55篇)。     

微流控技术应用于蛋白质结晶的研究*

随着微电子微机械等技术的不断进步,微流控(microfluidics)技术成为目前迅速发展的前沿领域之一,是化学科学和生命科学分析研究的重要技术平台。微流控技术高通量、低消耗和低成本的特点使其在蛋白质结晶条件筛选和优化方面展示了良好的应用前景。本文对应用于蛋白质结晶的各种微流控芯片技术的原理和方法进行了综述,并对目前几种商业化和文献报道的典型蛋白质结晶微流控系统进行了介绍和比较。     

精准入微——将微流控技术引入分析化学教学的探讨

微流控技术凭借其优异的微尺度操控能力,在分析化学和其他诸多领域得到了广泛而快速的发展。在本科阶段的分析化学课程引入微流控技术有助于提高分析化学教学的前沿性和趣味性,促进创新型人才的培养。本文回顾了微流控技术的发展史并挖掘了其中蕴含的课程思政元素,简要介绍了微流控技术的重要理论和微流控芯片的基本制作方法,讨论了微流控技术与分析化学的联系。在此基础上介绍了近年来微流控技术在分析化学领域,尤其是生命分析科学领域的研究进展,以期为分析化学教学引入微流控技术提供参考。     

基于微流控技术的蛋白质结晶及其筛选方法的研究进展简

微流控技术以其高通量、低消耗和集成化等优点成为蛋白质结晶微型化研究的重要手段. 本文综述了基于微流控技术的蛋白质结晶技术和方法,主要包括微泵微阀、液滴(Droplet)、滑动芯片(SlipChip)以及液滴实验室(DropLab)等技术. 此外,还针对当前膜蛋白在结构生物学研究中的重要地位,综述了应用于膜蛋白结晶的微流控技术的研究进展.     

基于微流控技术的蛋白质结晶及其筛选方法的研究进展

微流控技术以其高通量、 低消耗和集成化等优点成为蛋白质结晶微型化研究的重要手段. 本文综述了基于微流控技术的蛋白质结晶技术和方法, 主要包括微泵微阀、 液滴(Droplet)、 滑动芯片(SlipChip)以及液滴实验室(DropLab)等技术. 此外, 还针对当前膜蛋白在结构生物学研究中的重要地位, 综述了应用于膜蛋白结晶的微流控技术的研究进展.     

首届沈阳国际微流控学学术论坛（SICOM Ⅰ）第一轮会议通知

首届沈阳国际微流控学学术论坛(The First Shenyang International Colloquium on Microfluidics)将于2007年10月21~24日在沈阳举行。本届会议由沈阳市政府、国家自然科学基金委员会主办,东北大学承办。方肇伦院士担任会议主席。会议主要议题包括:微流控和纳流控操控技术及理论模型、微流控和纳流控元件及装置加工方法与技术、微流控芯片新材料及材料改性方法与技术、微流控系统的进样及试样处理与分离、微流控检测系统的微型化与集成化以及微流控系统在化学、生物学和医学中的应用。会议同时举办相关设备和产品的展览会。会议将邀请微流…     

首届沈阳国际微流控学学术论坛（SICOM I）第一轮会议通知

首届沈阳国际微流控学学术论坛(The First Shenyang International Colloquium on Microfluidics)将于2007年10月21~24日在沈阳举行。本届会议由沈阳市政府、国家自然科学基金委员会主办,东北大学承办。方肇伦院士担任会议主席。会议主要议题包括:微流控和纳流控操控技术及理论模型、微流控和纳流控元件及装置加工方法与技术、微流控芯片新材料及材料改性方法与技术、微流控系统的进样及试样处理与分离、微流控检测系统的微型化与集成化以及微流控系统在化学、生物学和医学中的应用。会议同时举办相关设备和产品的展览会。会议将邀请微流…     

整体柱富集技术在微流控芯片系统中的应用

样品预处理技术是微流控芯片技术发展的瓶颈之一。整体材料是近几年在色谱领域发展起来的一种新型色谱填料,具有结构均匀、传质速度快、通透性好、制备过程简单等优点,被广泛用于微流控芯片系统中。该文综述了整体柱富集技术在微流控芯片系统中的应用进展,引用文献80篇。     

微流控芯片在表面等离子体共振生物传感器中的应用

作为众所周知的生物传感器技术,表面等离子体共振（SurfacePlasmonResonance,SPR）正在被越来越普遍地用于实现各种生物化学检测方法,特别是用途广泛的固相表面生物检测（Sol—id—PhaseBioassay）。SPR对样品进行非标记检测,能够用于测量生物化学反应全过程的反应动力学。为了提高SPR的检测效率,通常将微流控技术（Microfluidics）与SPR相结合,即在SPR生物传感器中使用微流控芯片（MicrofluidicChip）作为反应装置。基于微型化带来的优势,使用微流控芯片作为反应装置可以有效地缩短生物化学检测方法的反应时间,并减少样品消耗。微流控芯片还可以平行排布相同的结构单元,提高SPR生物传感器的检测通量。因此,使用微流控芯片作为反应装置是SPR生物传感器,特别是商品化的SPR生物传感器的发展趋势。     

基于微流控热泳的生物传感技术※

复杂生命体系中关键分子及微纳生物粒子的高灵敏、高特异检测, 对理解多层次多尺度生物学过程、阐明疾病发生发展机制和探索新型生物标志物等具有重要意义. 微流控生物传感器整合了微流控技术和生物传感技术的诸多优势, 在微量生物样本精准测量方面取得了显著进展. 近年来, 微流控热泳生物传感技术(Thermomicrofluidic biosensing)利用物质在局域温度梯度场中的热泳定向迁移现象, 并结合均相生物传感及信号放大新策略, 实现了复杂样本中生物分子及微纳生物粒子的快速、高灵敏、原位检测. 重点阐述了以热泳为核心的微流控传感技术, 包括微量热泳、热泳-对流耦合、热泳-扩散泳耦合以及热泳-电泳耦合等方法, 总结了不同传感方法的原理、特点及其在生物分子(蛋白、核酸等)与微纳生物粒子(细胞外囊泡、病毒、细胞等)检测中的应用, 并探讨了微流控热泳技术在生物医学检测领域中面临的挑战与未来发展方向.     

Design of a zinc(II) ion specific fluorescence sensor

We report the synthesis of {[3-(biscarboxymethylamino)-2-methoxy-5-methylphenyl]carboxymethylamino}acetic acid, which functions as a Zn²⁺ selective fluorescence probe (sensor).     

A Zn(II) ion selective fluorescence sensor that is not affected by Cd(II)

Comparison of sensors sodium-2,6-diamino-(N,N,N′,N′-tetraacetate)-4-methylanisole 1 and sodium-2,6-diamino-(N,N,N′-triacetate)-4-methylanisole 2 reveal that the loss of an acetyl group in 2 leads to a more selective Zn(II) induced fluorescence enhancement and shows no response to any other metal ions including Cd(II). Structural modifications and AM1 calculations indicate that the sensor uses the three acetyl groups and the 3° amino nitrogen for binding the metal ion. AM1 calculations imply a trigonal bipyramidal coordination for Zn(II) with the solvent molecule occupying one of the axial positions.     

Recent trends of biosensors in Japan

Remarkable progress has been made in the development of bioelectrochemical sensors, and various kinds of biosensors have been developed and applied in analytical chemistry. Many of these enzyme, microbial, and immunosensors have been recently developed in Japan and in this paper, recent trends in Japanese biosensor development are described.     

Analytical characteristics and sensitivity mechanisms of electrolyte-insulator-semiconductor system-based chemical sensors—a critical review

Recent trends in research and development of electrolyte-insulator-semiconductor (EIS) field-effect chemical sensors (ion-selective field-effect transistors, light-addressable potentiometric sensors, capacitive EIS-sensors) with inorganic gate insulators (oxide, nitride and chalcogenide films) are reviewed. Physical properties of EIS systems and basic mechanisms of their chemical sensitivity are examined. Analytical characteristics and sensing mechanisms of EIS pH sensors with oxide and nitride films, as well as metal ions sensors with chalcogenide films, are critically discussed. Prospects of future research on EIS field-effect biosensors are briefly outlined.     

A mini-review on MXenes as versatile substrate for advanced sensors

MXenes have emerged as versatile 2D materials that are already gaining paramount attention in the areas of energy,catalyst,electromagnetic shielding,and sensors.The unique surface chemistry,graphene-like mo rphology,high hydrophilicity,metal-like conductivity with redox capability identifies MXenes,as an ideal material for surface-related applications.This short review summarizes the most recent reports that discuss the potential application of MXenes and their hybrids as a transducer material for advanced sensors.Based on the nature of transducing signals,the discussion is categorized into three sections,which include electrochemical(bio) sensors,gas sensors,and finally,electro-chemiluminescence fluorescent sensors.The review provides a concise summary of all the analytical merits obtained subsequent to the use of MXenes,followed by endeavors that have been made to accentuate the future perspective of MXenes in sensor devices.     

Optical sensors for determination of heavy metal ions

A review is given on optical means for single shot testing (probing) as well as continuous monitoring (sensing) of heavy metal ions (HMs). Following an introduction into indicator based approaches, we discuss the types of indicator dyes and polymeric supports used, as well as existing sensing schemes for HMs. The wealth of information is compiled in the form of tables and critically reviewed. Notwithstanding the tremendous work performed so far, it is obvious that still severe limitations do exist in terms of selectivity, limits of detection, dynamic ranges, applicability to specific problems, and reversibility. On the other hand, such sensors have found — and will find — their application whenever rapid and cost-effective testing is required, where personnel is scarce or unskilled, and in field tests. Despite their limitations, the number of such sensors (and of irreversible probes) for HMs is likely to increase in future.     

Electrochemical sensors for environmental gas analysis

The application of electrochemical sensors for measurement of concentration of pollutant gases in air in the part-per-billion (10⁹) range is reviewed. Performance-limiting factors, particularly the effects of extremes and of relatively rapid changes in ambient temperature and humidity, are noted. Variations in composition of the electrolyte in the meniscus at the electrode–gas interface and instability of the solid–liquid–gas contact line, causing important variations in current due to background electrode reactions, are deduced and suggested as the reason for the performance limitations. Suggestions are made for mitigation through instrument design.     

Transition metal and organometallic anion complexation agents

Anions are ubiquitous species, and therefore, their sensing is of considerable interest. Anion receptors containing electrochemically active groups such as ferrocene or cobaltocenium, or optically active groups such as ruthenium(II) bipyridyl derivatives, allow the binding of anions to be detected by a physical response at the metal centre. These systems have been incorporated into various acyclic, macrocyclic and calix[4]arene frameworks, many of which include an amide hydrogen-bonding group. Anions may be recognised in a range of environmental conditions, with some receptors even being active in aqueous solution. The incorporation of new transition metal and organometallic systems has led to the development of several new strategies in anion recognition.     

Novel Test System for Gas Sensing Materials and Sensors

Summary: A growing interest in gas sensors necessitates the requirement for a test system that is reliable and capable of handling multiple gas analytes at various concentrations. Most test systems currently used do not incorporate some type of standard with which to verify the actual concentrations of the gas analytes being tested, but rather calculate concentrations indirectly. The problem is that the calculated concentrations rarely take into account any loss that may occur across the test system. The test system described herein uses a specialized gas chromatograph (GC) that is capable of detecting down to the ppb range. It can either be used to screen potential sensing materials or to verify the actual concentration being tested for a specific sensor.