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1.
The noncovalent interaction of two anionic cyanine dyes with human and bovine serum albumins was studied by spectral and fluorescent methods. Upon the interaction with albumins, a growth of fluorescence and, in most cases, a long-wavelength shift of the dye absorption band are observed. For the meso-substituted cyanine dye 3,3′-di-(γ-sulfopropyl)-9-methylthiacarbocyanine betaine (K1), a mobile cis-trans equilibrium is observed: the dye in the free state occurs mainly as the cis-isomer, whereas in the complex with albumins the equilibrium is shifted toward the trans-isomer (this shift is greater for human albumin). Dye K1 is recommended as a spectral and fluorescent probe for serum albumins. 相似文献
2.
A comparative study of the noncovalent interaction of the cyanine dye probe 3,3′-di-(γ-sulfopropyl)-4,5,4′,5′-dibenzo-9-ethylthiacarbocyanine betaine with serum albumins of different vertebrates: rat, rabbit, bovine, and human serum albumins (RSA, TSA, BSA, and HSA, respectively) has been performed by spectral and fluorescent methods. It has been shown that, the dye forms only one product, the trans-monomer bound to HSA, by interacting with HSA, whereas other binding products are also formed with other albumins. This is probably explained by a higher interaction energy of the dye with HSA than with other serum albumins. 相似文献
3.
Farzaneh Farivar Ali Akbar Moosavi-Movahedi Saeed Yousefinejad Maryam Salami Ali Akbar Saboury Amir Niasari-Naslaji 《Journal of the Iranian Chemical Society》2014,11(5):1449-1457
Interactions of camel serum albumin (CSA) and human serum albumin (HSA) with sodium dodecyl sulphate (SDS) were studied via fluorescence and circular dichroism (CD) spectroscopy and tensiometry. There was a good correlation between fluorescence intensity and surface tension changes of CSA and HSA versus SDS concentration in which both showed two main transitions. However, the CD signals of CSA at 222 nm versus SDS concentration showed only one transition. According to the obtained results, interaction of SDS with the proteins can be divided into four distinct regions; in the initial region, SDS binds specifically to high-affinity binding sites, in the second region, SDS binds to the lower affinity binding sites on the protein surface, in the third region, cooperative binding of SDS triggers the protein to unfold. Finally after saturation of protein with SDS, at the final stage, normal SDS micelles start to form. Our results indicated that the partial unfolding of HSA started at higher concentrations of SDS and proceeded with higher cooperativity compared to that of CSA. This may be attributed to a higher inner hydrophobicity and higher stability of HSA compared to CSA. 相似文献
4.
荧光法研究喹诺酮类抗菌药物和蛋白质的相互作用 总被引:6,自引:2,他引:6
用荧光光谱法、分光光度法研究水溶液中甲磺酸培氟沙星、盐酸芦氟沙星与牛血清白蛋白(BSA)的相互结合反应,表明二者以摩尔比1∶1牢固结合,结合的平衡常数分别为KPM=7 3×104L mol、KRH=9 3×104L mol。根据F rster非辐射能量转移机理,求算了甲磺酸培氟沙星、盐酸芦氟沙星与牛血清白蛋白给体-受体间距离r分别为2 82nm,2 93nm,能量转移效率E分别为0 31,0 44。喹诺酮类药物与牛血清白蛋白的相互结合作用为单一的静态猝灭过程,其作用机制为能量转移机制。 相似文献
5.
Molecular spectroscopic study on the interaction of tetracyclines with serum albumins 总被引:10,自引:0,他引:10
Bi S Song D Tian Y Zhou X Liu Z Zhang H 《Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy》2005,61(4):629-636
A molecular spectroscopic investigation of the interaction between tetracyclines antibiotics and human serum albumin or bovine serum albumin was reported. The influences of some metal ions on the interaction were also studied. When tetracyclines drugs were added into the solution containing serum albumins, the fluorescence intensity of serum albumins decreased with the increasing of the drugs concentrations, which is due to the formation of new non-fluorescence complexes of drug-serum albumin. The tetracyclines acted as quenchers and quenched the fluorescence of the serum albumins. The binding constants and the number of the binding sites of the reaction of tetracyclines and serum albumins were obtained. The main sorts of acting force between the drugs and serum albumins were found and the action distances and the energy transfer efficiencies between donor-acceptor were calculated based on the Foster energy transference. 相似文献
6.
Farnaz Jafarpour Nafiseh Jalalimanesh Mina Barzegar Amiri Olia Asieh Otaredi Kashani 《Tetrahedron》2010,66(49):9508-9511
A simple and highly efficient protocol with mild reaction conditions has been developed that allows the smooth protiodecarboxylation of diversely functionalized coumarin-3-carboxylic acids. In the presence of catalytic amounts of Ag2CO3 and acetic acid, even un-activated coumarin-3-carboxylic acids were converted in good to excellent yields and with great preparative ease to the corresponding coumarin derivatives. 相似文献
7.
Spectroscopic studies on the interaction of bovine (BSA) and human (HSA) serum albumins with ionic surfactants 总被引:6,自引:0,他引:6
Gelamo EL Tabak M 《Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy》2000,56(11):2255-2271
Bovine (BSA) and human (HSA) serum albumins are frequently used in biophysical and biochemical studies since they have a similar folding, a well known primary structure, and they have been associated with the binding of many different categories of small molecules. One important difference of BSA and HSA is the fact that bovine albumin has two tryptophan residues while human albumin has a unique tryptophan. In this work results are presented for the interaction of BSA and HSA with several ionic surfactants, namely, anionic sodium dodecyl sulfate (SDS), cationic cethyltrimethylammonium chloride (CTAC) and zwitterionic N-hexadecyl-N,N-dimethyl-3-ammonium-1-propanesulfonate (HPS), as monitored by fluorescence spectroscopy of intrinsic tryptophans and circular dichroism spectroscopy. On the interaction of all three surfactants with BSA, at low concentrations, a quenching of fluorescence takes place and Stern-Volmer analysis allowed to estimate their 'effective' association constants to the protein: for SDS, CTAC and HPS at pH 7.0 these constants are, respectively, (1.4+/-0.1) x 10(5) M(-1), (8.9+/-0.1) x 10(3) M(-1) and (1.4+/-0.1) x 10(4) M(-1). A blue shift of maximum emission is observed from 345 to 330 nm upon surfactant binding. Analysis of fluorescence emission spectra allowed to separate three species in solution which were associated to native protein, a surfactant protein complex and partially denatured protein. The binding at low surfactant concentrations follows a Hill plot model displaying positive cooperativity and a number of surfactant binding sites very close to the number of cationic or anionic residues present in the protein. Circular dichroism data corroborated the partial loss of secondary structure upon surfactant addition showing the high stability of serum albumin. The interaction of the surfactants with HSA showed an enhancement of fluorescence at low concentrations, opposite to the effect on BSA, consistent with the existence of a unique buried tryptophan residue in this protein with considerable static quenching in the native state. The effects of surfactants at low concentrations were very similar to those of myristic acid suggesting a non specific binding through hydrophobic interaction modulated by eletrostatic interactions. The changes in the vicinity of the tryptophan residues are discussed based on the recently published crystallographic structure of HSA myristate complex (S. Curry et al., Nat. Struct. Biol. 5 (1998) 827). 相似文献
8.
Bourhim M Rajendran A Ramos Y Srikrishnan T Sulkowski E 《Journal of chromatographic science》2008,46(6):574-576
An investigation of the binding of native and recombinant human serum albumin and bovine serum albumin on three thiophilic gels, PyS, 2S, and 3S was performed. In addition to these proteins, we studied serum albumins from several species such as goat, rabbit, guinea pig, rat, hamster, baboon, and pig. Our results reveal that recombinant human serum albumin (rHSA) binds completely to PyS whereas native human serum albumin and bovine serum albumin bind only partially to PyS. The binding affinities of rHSA, human serum albumin and bovine serum albumin to 2S and 3S gels are less than their binding to PyS. Serum albumins from goat, rabbit, guinea pig, rat, hamster, baboon, and pig bind much stronger to 3S gel than human and bovine serum albumins. The binding of pig and hamster serum albumins is stronger than that of rat, goat, baboon, and rabbit. 相似文献
9.
《Journal of photochemistry and photobiology. A, Chemistry》2007,185(2-3):271-276
The interaction of 18β-glycyrrhetinic acid (GA): The metabolite of glycyrrhizic acid which is the main active component of a commonly used traditional Chinese medicine (TCM) Glycyrrhiza Uralensis Fisch with bovine serum albumin (BSA) has been investigated. Fluorescence emission spectra of serum albumin in the presence of GA, recorded at the excitation wavelength 280 nm, clearly show that GA act as quencher and have different quenching mechanism at a pH below or above the isoelectric point (pI). The binding sites number n and apparent binding constant K were measured. The thermodynamic parameters ΔH°, ΔG°, ΔS° at different temperatures were calculated. The effects of some common metal ions on binding are considered. Synchronous fluorescence and UV–vis spectra were used to study protein conformation. Energy transfer between GA and HSA was calculated by Förster's theory and the binding site was suggested to be site II. The binding of monoammonium glycyrrhizinate (GL) to BSA is also compared. 相似文献
10.
B. B. Koleva R. D. Nikolova S. Zareva T. Kolev A. G. Bojilova H. Mayer-Figge W. S. Sheldrick 《Structural chemistry》2008,19(6):975-982
IR-spectroscopic characterization of the coumarin-3-phosphonic acid and 1,2-benzoxaphosphorine-3-carboxylic acid ethyl esters has been carried out by means of linear-polarized IR (IR-LD) spectroscopy of oriented colloid suspensions in a nematic host. Quantum chemical DFT calculations at the B3LYP level of theory and 6-311++G** basis set were performed. The electronic structure and vibrational properties of both compounds are discussed. The spectroscopic data for 2-benzoxaphosphorine-3-carboxylic acid ethyl ester are in accordance with the crystal structure determined by single crystal X-ray diffraction. The compound C13H15O5P crystallizes in the noncentrosymmetric space group P212121, and its structure consists of a 3D network formed by short contacts of the type P=O···HC(Ar) with distances of 3.420 and 2.467 Å. The geometry of the PO3C fragment exhibits a pseudo T d symmetry. 相似文献
11.
光谱法研究甲芬那酸与蛋白质的相互作用 总被引:9,自引:0,他引:9
用UV Vis吸收光谱和荧光光谱法研究了在模拟人体生理条件下,甲芬那酸与牛血清白蛋白结合反应特征,发现甲芬那酸对牛血清白蛋白有较强的荧光猝灭作用,且甲芬那酸的紫外吸收光谱和牛血清白蛋白的荧光发射光谱有一定程度的重叠,由此得出了其结合反应的结合常数、结合位点数和结合过程的基本热力学参数。 相似文献
12.
The interaction of cinoxacin (CINX), nalidixic acid (NA), and pipemidic acid (PPA) with human and rat serum albumins (HSA and RSA) was studied by UV difference absorption and circular dichroism (CD) spectroscopy. CINX and NA bound to the albumins and generated difference absorption and induced CD (ICD) spectra. The difference absorption spectral data explained reasonably our previous observations that CINX bound to HSA more weakly than NA, but to RSA as strongly as NA. We used a quantity delta epsilon/epsilon, designated as relative molar difference absorbance, at positions corresponding to the longest wavelength peaks in the difference spectra. The quantity was found to correlate linearly with percent bound to both HSA and RSA, but with different slopes, from which the binding site for CINX and NA in RSA was supposed to provide a much more nonpolar environment than that in HSA. The magnitude of ICD bands observed at 371 nm for CINX and at 342-348 nm for NA corresponded to the binding degrees of these drugs to both albumins. Anisotropy factors for the ICD bands at 350-271 nm for CINX and 320-348 nm for NA were approximately similar between HSA and RSA, suggesting a similar ability to generate the ICD spectra in these wavelength regions upon binding to the albumins. Spectral results for PPA in albumin solutions showed little or no binding of this drug to HSA and RSA. PPA existed as a betaine form in neutral solution and its positively charged group acted as an unfavorable factor for binding to both albumins. 相似文献
13.
Singh TS Mitra S 《Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy》2011,78(3):942-948
Cinnamic acid (CA) derivatives are known to possess broad therapeutic applications including anti-tumor activity. The present study was designed to determine the underlying mechanism and thermodynamic parameters for the binding of two CA based intramolecular charge transfer (ICT) fluorescent probes, namely, 4-(dimethylamino) cinnamic acid (DMACA) and trans-ethyl p-(dimethylamino) cinnamate (EDAC), with albumins by fluorescence spectroscopy. Stern-Volmer analysis of the tryptophan fluorescence quenching data in presence of the added ligand reveals fluorescence quenching constant (κ(q)), Stern-Volmer constant (K(SV)) and also the ligand-protein association constant (K(a)). The thermodynamic parameters like enthalpy (ΔH) and entropy (ΔS) change corresponding to the ligand binding process were also estimated. The results show that the ligands bind into the sub-domain IIA of the proteins in 1:1 stoichiometry with an apparent binding constant value in the range of 10(4) dm(3) mol(-1). In both the cases, the spontaneous ligand binding to the proteins occur through entropy driven mechanism, although the interaction of DMACA is relatively stronger in comparison with EDAC. The temperature dependence of the binding constant indicates the induced change in protein secondary structure. 相似文献
14.
The interaction between cinnamic acid and bovine serum albumin (BSA) have been studied at three temperatures, 296, 303 and 310 K. Fluorescence quenching spectra in combination with Fourier transform infrared (FT-IR) spectroscopy and circular dichroism (CD) spectroscopy was used to investigate the drug-binding mode, the binding constant and the protein structure changes in the presence of cinnamic acid in aqueous solution at pH 7.40. The fluorescence quenching constant K(q), K(sv) and the binding constant K were calculated according to Stern-Volmer equation based on the quenching of the fluorescence of BSA in the presence of cinnamic acid. The thermodynamic parameters, the enthalpy (DeltaH) and the entropy change (DeltaS) were estimated to be -16.457 kJ mol(-1) and 38.028 J mol(-1) K(-1) according to the van't Hoff equation. The displacement experiment shows that cinnamic acid can bind to the subdomain IIA (corresponding to Sudlow's drug binding site I). The distance between the tryptophan residues in BSA and cinnamic acid bound to site I was estimated to be 1.63 nm using F?ster's equation on the basis of fluorescence energy transfer. The decreased binding constant in the presence of common ions indicates that common ions have effect on drug-BSA system. 相似文献
15.
A. S. Tatikolov A. A. Ishchenko M. A. Kudinova I. G. Panova 《High Energy Chemistry》2010,44(4):304-310
The noncovalent interaction of zwitterionic indolium squarylium dyes (hydrophilic and hydrophobic) and a structurally analogous
ionic indodicarbocyanine (hydrophilic) dye with serum albumins was studied by spectral and fluorescent methods. It has been
found that the hydrophilic squarylium dye with sulfonate groups most efficiently interacts with albumins, which is probably
due to the double negative charge of the dye molecule at the expense of the sulfonate groups and the possibility to form hydrogen
bonds with albumin. The hydrophobic squarylium dye, as well as the hydrophilic indodicarbocyanine dye without the squarylium
fragment in its structure, bind with albumins much weaker than the structurally relevant hydro- philic squarylium dye. The
properties of the latter dye permit us to recommend it for using as a spectral and fluorescent probe for serum albumins in
extracellular media of living organisms. 相似文献
16.
17.
The interaction of lanthanide(III) cations (Ln(III) = Sm(III), Eu(III), and Tb(III)) with the deprotonated form of the coumarin-3-carboxylic acid (cca-) has been investigated by density functional theory (DFT/B3LYP) and confirmed by reference MP2 and CCSD(T) computations. Solvent effects on the geometries and stabilities of the Ln(III) complexes were computed using a combination of water clusters and a continuum solvation model. The following two series of systems were considered: (i) Ln(cca)2+, Ln(cca)2+, Ln(cca)3 and (ii) Ln(cca)(H2O)2Cl2, Ln(cca)2(H2O)2Cl, Ln(cca)3. The strength and character of the Ln(III)-cca- bidentate bonding were characterized by calculated Ln-O bond lengths, binding energies, ligand deformation energies, energy partitioning analysis, sigma-donation contributions, and natural population analyses. The energy decomposition calculations predicted predominant electrostatic interaction terms to the Ln-cca bonding (ionic character) and showed variations of the orbital interaction term (covalent contributions) for the Ln-cca complexes studied. Electron distribution analysis suggested that the covalent contribution comes mainly from the interaction with the carboxylate moiety of cca-. 相似文献
18.
The interactions between oleanolic acid and bovine serum albumin (BSA) have been studied by fluorescence, circular dichroism (CD), UV–vis absorption and Fourier transform infrared spectroscopy (FTIR) under physiological conditions. Spectroscopic analysis of the emission quenching at different temperatures has revealed that the quenching mechanism of bovine serum albumin by oleanolic acid is static quenching mechanism. The binding sites number n and binding constants K are obtained at various temperatures. The distance r between oleanolic acid and the protein is evaluated according to the theory of Forster energy transfer. The results by FTIR, CD and UV–vis absorption spectra experiment indicate that the secondary structures of protein have been perturbed in the presence of oleanolic acid. The thermodynamic parameters ΔH0, ΔG0, and ΔS0 are calculated according to van’t Hoff equation, which indicates that the hydrogen bonds and van der-waals are the intermolecular forces stabilizing the complex. Molecular modeling studies the interaction BSA with oleanolic acid. 相似文献
19.
The excited-state photophysical properties of curcumin in the presence of bovine serum albumin (BSA) have been studied. The absorption and fluorescence changes in curcumin on binding to BSA have been followed at varying concentrations of either curcumin or BSA to determine the binding constant, which has been found to be approximately 10(4) to 10(5) M(-1). Stopped-flow kinetics studies suggested at least two distinct kinetic steps for the binding of curcumin to BSA. The photophysical properties of the singlet-excited state of the curcumin-BSA complex have also been studied. Whereas the absorption spectrum of curcumin is redshifted, the fluorescence spectrum of curcumin was blueshifted in the presence of BSA. The fluorescence quantum yield of curcumin on complexing with BSA was approximately 0.05. Steady-state fluorescence anisotropy studies showed a significant increase in the anisotropy value of 0.37 in BSA-bound curcumin. The fluorescence decay of the curcumin-BSA complex followed a biexponential decay with fluorescence lifetimes of 413 ps (33%) and 120 ps (67%). On the basis of these complementary results, it has been concluded that curcumin shows very high binding to BSA, probably at the hydrophobic cavities inside the protein. 相似文献
20.
Binding of the vitamin folic acid with bovine serum albumin (BSA) has been studied using isothermal titration calorimetry (ITC) in combination with fluorescence and circular dichroism spectroscopies. The thermodynamic parameters of binding have been evaluated as a function of temperature, ionic strength, in the presence of nonionic surfactants triton X-100, tetrabutylammonium bromide, and sucrose. The values of the van’t Hoff enthalpy calculated from the temperature dependence of the binding constant agree with the calorimetric enthalpies indicating that the binding of folic acid to the BSA is a two state process without involving intermediates. These observations are supported by the intrinsic fluorescence and circular dichroism spectroscopic measurements. With increase in the ionic strength, reduction in the binding affinity of folic acid to BSA is observed suggesting predominance of electrostatic interactions in the binding. The contribution of hydrophobic interactions in the binding is also demonstrated by decrease in the binding affinity in the presence of tetrabutylammonium bromide (TBAB). The value of binding affinity in the presence of sucrose indicates that hydrogen bonding also plays a significant contribution in the complexation process. The calorimetric and spectroscopic results provide quantitative information on the binding of folic acid to BSA and suggest that the binding is dominated by electrostatic interactions with contribution from hydrogen bonding. 相似文献