Spectrophotometric studies on the uranyl-ascorbate complex

A systematic spectrophotometric study on the complexation of uranyl ions with ascorbic acid (vitamin C) is carried out. Factors influencing the sensitivity or selectivity of uranyl ascorbate are studied, and adequate elimination or control of the procedure is suggested. Results of the absorbance of uranyl ascorbate developed in HNO₃, HCl, and H₂SO₄ acid media within the concentration range (1.0–8.0) × 10⁻N show a marked dependence on the nature and concentration of the acid used. The observed decrease in color intensity at higher acidities is explained on the basis of the decomposing effect of hydrogen ions on uranyl ascorbate and the complexing effect on uranyl ions. Study of the stoichiometry of uranium:ascorbic acid complex in acid medium by continuous variation and molar ratio methods indicated the formation of 1:1 and 1:2 complexes. The formation constant of the 1:1 complex as calculated by these two methods was found to be 4.8 × 10⁴ and 5.0 × 10⁴, respectively. Best suitable conditions for stabilizing and increasing the color intensity of uranyl ascorbate, dependent on the nature of the medium and pH of the solution, are suggested for the determination of ascorbic acid in pharmaceutical preparations using this method.     

Spectrophotometric studies on platinum—Promethazine hydrochloride complex

Promethazine hydrochloride forms a yellowish-green complex with platinum(IV) in sodium acetate-hydrochloric acid buffer containing copper catalyst. The complex has an absorption maximum at 406 nm with molar absorptivity 1.001 × 10⁴ liters mol^?1 cm^?1. The sensitivity of the reaction is 19.5 ng/cm² for $\text{log}\text{I °}\text{I}\text{= 0.001}$. Beer's law is valid over the range 0.5–7.5 ppm of platinum(IV) with optimum concentration range 0.8-7.3 ppm. Continuous variations, mole ratio, and slope ratio methods indicate 1:1 composition for the complex. The effects of pH, time, temperature, order of addition of reagents, reagent concentration, and interferences from various ions are reported.     

Spectrophotometric studies on the platinum(IV)-prochlorperazine bismethanesulfonate complex

We have studied the formation of a platinum complex and developed a simple, rapid and sensitive spectrophotometric method for the determination of platinum in solution. The method is based on the complexation reaction of the chromogen, prochlorperazine bismethane-sulfonate (PCPMS), with platinum(IV) in phosphoric acid medium which forms a reddish brown 1 1 complex with an absorption maximum around 528 nm. The reaction is fast in the presence of copper(II) and goes to completion within 1 min. Beer's law is obeyed over the concentration range 0.3–7.2 g/ml of platinum(IV) with an optimal range of 1.2–6.6 g/ml. The molar absorptivity is 2.65 × 10⁴1 mol^–1 cm^–1 and the Sandell's sensitivity is 7.8 ng cm^–2. The stability constant, logK, of the complex is 4.96±0.1 at 25 ° C. The effects of time, temperature, concentrations of acids, PCPMS and copper(II), and the interference by various ions are investigated. The method has been successfully applied to the determination of platinum content in alloys and minerals.     

Spectrophotometric solvent extraction studies of 2-mercaptopyridine-1-oxide and uranyl ion complex

The complex formed by uranyl ion with 2-mercaptopyridine-1-oxide (thione) is studied by spectrophotometry. At pH 4.5 and 25 degrees the only complex formed is UO(2)(thione)(3)(-). The conditional stability constant of this complex is 2.6 x 10(12). Tributyl phosphate is used for the extraction of the complex from the aqueous phase and the extraction constant is 8.9 x 10(12). The use of the reagent for the separation and determination of uranyl ion is elaborated.     

Spectrophotometric study of the inclusion complex between beta-cyclodextrin and dibenzoyl peroxide and its analytical application

The noncovalent interaction of dibenzoyl peroxide and beta-cyclodextin (beta-CD) has been studied by spectrophotometry. The mechanism of the inclusion was studied. The results showed that beta-CD reacts with dibenzoyl peroxide to form a 2:1 host-guest complex with an apparent formation constant of 2.5 x 10(4)mol(-2)L(2). The beta-CD reacts with benzoic acid to form a 1:1 host-guest complex with an apparent formation constant of 6.9 x 10(2)mol(-1)L after the dibenzoyl peroxide was reduced by hydroxyl ammonium. Based on the enhancement of the absorbance of dibenzoyl peroxide produced through complex formation, a spectrophotometric method for determination of dibenzoyl peroxide in bulk aqueous solution in the presence of beta-CD was developed. A linear relationship between the absorbance and dibenzoyl peroxide concentration was obtained in the range of 0.300-50.0 microg mL(-1). Linear regression equation of the calibration graph C=0.02926+53.25 A, with a correlation coefficient of 0.9984 and a relative standard deviation (R.S.D.) of 3.4%. The detection limit was 0.200 microg mL(-1), and the recovery was from 98.00 to 105.0%. The proposed method was used to determine the dibenzoyl peroxide in the flour with satisfactory results. The principal advantage of the proposed method is its excellent selectivity based on molecule recognition of beta-CD.     

Spectrophotometric studies on the solvatochromism of the Fe(CN)2(phen)2 complex

Summary The solvatochromic behaviour ofbis-(cyano)-bis-(9,10-phenanthroline)-iron(II) has been reexamined spectrophotometrically in a series of 19 solvents including water, alcohols and dipolar. aprotic media. Satisfactory correlations between the wave numbers of the long-wavelength band and solvent acceptor numbers are obtained in all media; only values, for carboxylic acids deviate from the regression line.

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Spektrophotometrische Untersuchungen über die Solvatochromie der Komplexverbindung Fe(CN)₂(phen)₂

Zusammenfassung Das solvatochrome Verhalten vonbis-(Cyano)-bis-(9,10-phenanthrolin)-eisen(II) wurde in 19 Lösungsmitteln, darunter Wasser, Alkohole und dipolare aprotische Medien, spektrophotometrisch überprüft. In allen Medien wurden zufriedenstellende Korrelationen zwischen den Wellenzahlen der langwelligen Bande und den Akzeptorzahlen der Lösungsmittel erhalten; lediglich die Werte für Carbonsäuren weichen von der Ausgleichgeraden ab.

     

Spectrophotometric study of the beryllium-thorin complex and its application to the determination of beryllium in alloys

A spectrophotornetric study of the beryllium complex with purified thorin shows the formation of a I : I complex at pH 12. The dissociation constant obtained after successive corrections for reagent absorbance is 1.38·10^-7. Beryllium in concentrations of 0.05% to 2.0% in copper base, zinc base, aluminiuin base and ferrous alloys can be determined by the formation of the thorin complex after its preliminary separation as accetylacetonate in presence of EDTA.     

Spectrophotometric studies of neomycin-copper complex and determination of neomycin sulfate using an auxiliary ligand

Spectrophotometric investigation of the deep blue colored, water-soluble complex of

1. Stability constant and free energy of formation of Cu-NMS complex at 30 ± 1 °C

     

11.

Spectrophotometric studies of kanamycin-copper complex and determination of kanamycin sulfate using an auxiliary ligand     

《Journal of Inorganic and Nuclear Chemistry》1978,40(9):1715-1718

Spectrophotometric investigation of the deep blue, water soluble complex of kanamycin sulphate with bivalent copper in the alkaline medium has been carried out using sodium potassium tartrate as an auxiliary ligand. The complex is found to absorb maximum at 580 nm and pH 8.0. Job's method and mole ratio method have shown the composition of the complex to be 1:2. Conditional stability constant has been determined by Mukherji and Dey method and Raghav Rao's method and the log K_s value by these two methods is found to be 6.69 and 7.59 respectively. The colour of the complex obeys Beer's law within the range 0.3 – 3.0 mg of kanamycin sulphate/ml of the solution. The technique is found to be suitable for the quantitative estimation of kanamycin sulphate.     

12.

Spectrophotometric studies on amylose-iodine and amylopectin-iodine complexes     

U. K. SenGupta  A. K. MuKherjee  K. K. SenGupta 《Colloid and polymer science》1966,208(1):32-34

Summary Starch isolated from water-chestnut (Trapa Bispinosa Roxb) was fractionated into amylose and amylopectin by conventional method. Spectrophotometric studies have been carried on each of amyloseiodine and amylopectin-iodine complex. The kinetics of the decomposition of these complexes have also been reported.

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Zusammenfassung Stärke isoliert aus Wasser Kastanie (Trapa Bispinosa Roxb) wurde in Amylose und Amylopektin mit konventioneller Methodik fraktioniert. An beiden, dem Amylose-Jod und Amylopektin-Jod-Komplex wurden spektrometrische Diagramme aufgenommen. Auch über die Kinetik des Abbaues dieser Komplexe wird berichtet.

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Thanks are due to Prof.P. C. Dutta, Department of Macromolecules, Indian Association for the Cultivation of Science and Prof.B. N. Ghosh, Head of the Chemistry Department of Calcutta University for their interests in the work.     

13.

Spectrophotometric study of copper-formazan complex     

I. I. Elbeih  A. S. Shawali  M. A. Abotabl 《Analytical and bioanalytical chemistry》1972,258(2):124-125

     

14.

Spectrophotometric study of copper-formazan complex     

I. I. Elbeih  A. S. Shawali  M. A. Abotabl 《Fresenius' Journal of Analytical Chemistry》1972,261(2):124-125

     

15.

Spectrophotometric studies of molecular complex formation between water-soluble cobalt(II) Schiff base complex and nucleotides in mixed solvent systems     

Boghaei DM  Gharagozlou M 《Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy》2005,61(13-14):3061-3065

The formation constants for 1:1 molecular complex formation between water-soluble cobalt(II) tetradentate Schiff base complex, disodium[{bis(4-methoxy-5-sulfo-salicylaldehyde)-4,5-dimethyl-o-phenylenediiminato}cobalt(II)], Na2[Co(SO3-4-meosal-4,5-dmophen)], and nucleotides, adenosine-5'-triphosphate (ATP) and cytidine-5'-triphosphate (CTP), in mixed solvent systems of ethanol and water with different volume fractions of ethanol and water have been determined spectrophotometrically at constant ionic strength (I = 0.2 mol dm(-3) NaClO4) and temperature 278 K. Trends in the values of formation constants according to the volume fractions of ethanol and water in ethanol and water mixed solvent systems, suggest that the trend of molecular complex formation increases with increasing the volume fraction of ethanol in mixed solvent systems.     

16.

Spectrophotometric investigations of the complex of Lead2+ and rutin     

Z. Radović  D. Malešev 《Mikrochimica acta》1985,86(3-4):247-252

Summary Investigations of Pb(II)-rutin complex have been carried out in ethanol, at pH=4.5 and at room temperature. It has been found that a relatively stable complex of yellow-green colour is formed; the molar ratio Pb(II): rutin=12 in the complex, was established by Job's and Nach's method as well as by the method of molar ratios. By applying Sommer's and Nach's method, the relative stability constant of the complex was obtained (log ₂= 6.48–7.05). Conditions for the quantitative determination of Pb(II) and rutin, respectively, by means of the complex, are presented as well.     

17.

Spectrophotometric determination of platinum using the diethazine hydrochloride complex     

H. Sanke Gowda  K. S. Jagadeesh 《Mikrochimica acta》1979,71(3-4):183-190

Summary Diethazine hydrochloride forms an yellowish-green complex with platinum(IV) in sodium acetate-hydrochloric acid buffer containing copper catalyst. The complex has an absorption maximum at 404nm and its molar absorptivity is 1.083×10⁴l mole^–1cm^–1. The sensitivity is 18 ng/cm² for logI ₀/I=0.001. Beer's law is valid over the concentration range 0.5–12.0 ppm. The composition of the complex as determined by the continuous variations, mole ratio and slope ratio methods is 11 and the apparent stability constant of the complex at pH 2.3 and 27° has the log K value of 5.83. The effects due to pH, reagent concentration, copper concentration, time and diverse ions are reported.

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Zusammenfassung 10-(2-Diäthylaminoäthyl)-phenothiazinhydrochlorid (DH) bildet mit Platin in Natriumacetat-Salzsäure-Puffer, der Kupfer als Katalysator enthält, eine gelbgrüne Komplexverbindung. Diese hat ein Absorptionsmaximum bei 404 nm, ihre molare Extinktion beträgt 1,083×10⁴l·mol^–1·cm^–1. Die Empfindlichkeit entspricht 18 ng/cm² für logI ₀/I=0,001. Das Beersche Gesetz gilt zwischen 0,5 und 12,0 ppm. Der Komplex ist im Verhältnis 11 zusammengesetzt. Die scheinbare Stabilitätskonstante bei pH 2,3 und 27° C entspricht logK=5,83. Über den Einfluß von pH, Reagenskonzentration, Kupferkonzentration, Zeit und verschiedene Fremdionen wurde berichtet.

     

18.

Spectrophotometric studies of reduced molybdoantimonylphosphoric acid     

J.E. Going  S.J. Eisenreich 《Analytica chimica acta》1974,70(1):95-106

Spectrophotometric studies have been used to determine the optimal formation conditions and stoichiometry of the reduced molybdoantimonylphosphoric acid. It was found that a [H⁺]/[MoO^2-₄] ratio of70± 10 was optimal for formation between 0.0008 and 0.01 M molybdenum. This corresponds to solution conditions that favor the existence of the dimeric form of molybdenum. The [H⁺]/[MoO^2-₄] criteria allows simple variation of experimental conditions. The stoichiometry of the reduced heteropoly acid was surmised to be PSb₂Mo₁₀O₄₀ from spectral studies and elemental analysis.     

19.

电荷转移分光光度法测定氧氟沙星含量的研究     

全红  白小红  宋艳红 《分析试验室》2002,21(4):64-66

利用氧氟沙星与对硝基苯酚形成荷转移化合物，且该化合物在403nm处有强吸收，而药物氧氟沙星在此处无吸收的特点，研究了氧氟沙星－对硝基苯酚荷转移化合物的吸收系数，形成比以及离解常数，并对氧氟沙星原料药进行了含量测定，其回收率为99．5％，相对标准偏差3．4％。     

20.

Spectrophotometric determination of salicylamide in complex mixtures     

L. Szekeres  R.E. Harmon  S.K. Gupta 《Microchemical Journal》1973,18(2):101-106

This paper describes a photometric assay procedure for the determination of small amounts of salicylamide in the presence of a variety of foreign substances. This method is based on the formation of a blue colored indophenol derivative from the reaction of salicylamide with N,2,6-trichloro-p-benzoquinoneimine.     

Method	$\text{log}\text{K}{}_{\mathrm{s}}$	ΔF (Kcal/mole)
Mukherji and Dey (4)	4.11	?5.70
Subhrana and Raghavrao (6)	4.80	?6.66

Spectrophotometric studies of kanamycin-copper complex and determination of kanamycin sulfate using an auxiliary ligand

Spectrophotometric investigation of the deep blue, water soluble complex of kanamycin sulphate with bivalent copper in the alkaline medium has been carried out using sodium potassium tartrate as an auxiliary ligand. The complex is found to absorb maximum at 580 nm and pH 8.0. Job's method and mole ratio method have shown the composition of the complex to be 1:2. Conditional stability constant has been determined by Mukherji and Dey method and Raghav Rao's method and the log K_s value by these two methods is found to be 6.69 and 7.59 respectively. The colour of the complex obeys Beer's law within the range 0.3 – 3.0 mg of kanamycin sulphate/ml of the solution. The technique is found to be suitable for the quantitative estimation of kanamycin sulphate.     

Spectrophotometric studies on amylose-iodine and amylopectin-iodine complexes

Summary Starch isolated from water-chestnut (Trapa Bispinosa Roxb) was fractionated into amylose and amylopectin by conventional method. Spectrophotometric studies have been carried on each of amyloseiodine and amylopectin-iodine complex. The kinetics of the decomposition of these complexes have also been reported.

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Zusammenfassung Stärke isoliert aus Wasser Kastanie (Trapa Bispinosa Roxb) wurde in Amylose und Amylopektin mit konventioneller Methodik fraktioniert. An beiden, dem Amylose-Jod und Amylopektin-Jod-Komplex wurden spektrometrische Diagramme aufgenommen. Auch über die Kinetik des Abbaues dieser Komplexe wird berichtet.

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Thanks are due to Prof.P. C. Dutta, Department of Macromolecules, Indian Association for the Cultivation of Science and Prof.B. N. Ghosh, Head of the Chemistry Department of Calcutta University for their interests in the work.     

Spectrophotometric studies of molecular complex formation between water-soluble cobalt(II) Schiff base complex and nucleotides in mixed solvent systems

The formation constants for 1:1 molecular complex formation between water-soluble cobalt(II) tetradentate Schiff base complex, disodium[{bis(4-methoxy-5-sulfo-salicylaldehyde)-4,5-dimethyl-o-phenylenediiminato}cobalt(II)], Na2[Co(SO3-4-meosal-4,5-dmophen)], and nucleotides, adenosine-5'-triphosphate (ATP) and cytidine-5'-triphosphate (CTP), in mixed solvent systems of ethanol and water with different volume fractions of ethanol and water have been determined spectrophotometrically at constant ionic strength (I = 0.2 mol dm(-3) NaClO4) and temperature 278 K. Trends in the values of formation constants according to the volume fractions of ethanol and water in ethanol and water mixed solvent systems, suggest that the trend of molecular complex formation increases with increasing the volume fraction of ethanol in mixed solvent systems.     

Spectrophotometric investigations of the complex of Lead2+ and rutin

Summary Investigations of Pb(II)-rutin complex have been carried out in ethanol, at pH=4.5 and at room temperature. It has been found that a relatively stable complex of yellow-green colour is formed; the molar ratio Pb(II): rutin=12 in the complex, was established by Job's and Nach's method as well as by the method of molar ratios. By applying Sommer's and Nach's method, the relative stability constant of the complex was obtained (log ₂= 6.48–7.05). Conditions for the quantitative determination of Pb(II) and rutin, respectively, by means of the complex, are presented as well.     

Spectrophotometric determination of platinum using the diethazine hydrochloride complex

Summary Diethazine hydrochloride forms an yellowish-green complex with platinum(IV) in sodium acetate-hydrochloric acid buffer containing copper catalyst. The complex has an absorption maximum at 404nm and its molar absorptivity is 1.083×10⁴l mole^–1cm^–1. The sensitivity is 18 ng/cm² for logI ₀/I=0.001. Beer's law is valid over the concentration range 0.5–12.0 ppm. The composition of the complex as determined by the continuous variations, mole ratio and slope ratio methods is 11 and the apparent stability constant of the complex at pH 2.3 and 27° has the log K value of 5.83. The effects due to pH, reagent concentration, copper concentration, time and diverse ions are reported.

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Zusammenfassung 10-(2-Diäthylaminoäthyl)-phenothiazinhydrochlorid (DH) bildet mit Platin in Natriumacetat-Salzsäure-Puffer, der Kupfer als Katalysator enthält, eine gelbgrüne Komplexverbindung. Diese hat ein Absorptionsmaximum bei 404 nm, ihre molare Extinktion beträgt 1,083×10⁴l·mol^–1·cm^–1. Die Empfindlichkeit entspricht 18 ng/cm² für logI ₀/I=0,001. Das Beersche Gesetz gilt zwischen 0,5 und 12,0 ppm. Der Komplex ist im Verhältnis 11 zusammengesetzt. Die scheinbare Stabilitätskonstante bei pH 2,3 und 27° C entspricht logK=5,83. Über den Einfluß von pH, Reagenskonzentration, Kupferkonzentration, Zeit und verschiedene Fremdionen wurde berichtet.

     

Spectrophotometric studies of reduced molybdoantimonylphosphoric acid

Spectrophotometric studies have been used to determine the optimal formation conditions and stoichiometry of the reduced molybdoantimonylphosphoric acid. It was found that a [H⁺]/[MoO^2-₄] ratio of70± 10 was optimal for formation between 0.0008 and 0.01 M molybdenum. This corresponds to solution conditions that favor the existence of the dimeric form of molybdenum. The [H⁺]/[MoO^2-₄] criteria allows simple variation of experimental conditions. The stoichiometry of the reduced heteropoly acid was surmised to be PSb₂Mo₁₀O₄₀ from spectral studies and elemental analysis.     

电荷转移分光光度法测定氧氟沙星含量的研究

利用氧氟沙星与对硝基苯酚形成荷转移化合物，且该化合物在403nm处有强吸收，而药物氧氟沙星在此处无吸收的特点，研究了氧氟沙星－对硝基苯酚荷转移化合物的吸收系数，形成比以及离解常数，并对氧氟沙星原料药进行了含量测定，其回收率为99．5％，相对标准偏差3．4％。     

Spectrophotometric determination of salicylamide in complex mixtures

This paper describes a photometric assay procedure for the determination of small amounts of salicylamide in the presence of a variety of foreign substances. This method is based on the formation of a blue colored indophenol derivative from the reaction of salicylamide with N,2,6-trichloro-p-benzoquinoneimine.