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1.
Jiaoyun Xia Shu Long Junfeng Yu Yongxian Wang Zhong Cao 《Journal of Radioanalytical and Nuclear Chemistry》2009,281(3):493-499
The purpose of this work was to indirect label IgG with fac-[188Re(CO)3(H2O)3]+ and to check the radiochemical behavior of the labeled product. The compound of (bis(2-pyridylmethyl)-amino)-acetic acid
(L2H) was synthesized and labeled with fac-[188Re(CO)3(H2O)3]+. The labeling yield of 188Re(CO)3–L2H was more than 90%. The effects of protein concentration, reaction time, pH and reaction temperature of labeling of IgG with
188Re(CO)3–L2H were investigated. The conjugation conditions were optimized. The labeled product was analyzed by size exclusion HPLC and
TLC. The stability of 188Re(CO)3–L2H–IgG in vitro was high. The results of this study may be useful for [188Re(CO)3(H2O)3]+ labeling of protein for radioimmunotherapy. 相似文献
2.
Jiaoyun Xia Yongxian Wang Gucai Li Junfeng Yu Duanzhi Yin 《Journal of Radioanalytical and Nuclear Chemistry》2009,279(1):245-252
In this paper, we investigated three ligand systems, symmetric and asymmetric pyridyl-containing tridentate ligands (L1NH2 = (bis(2-pyridylmethyl)-amino)-ethylamine, L2H = (bis(2-pyridylmethyl)-amino)-acetic acid, L3NH2 = [(6-amino-hexyl)-pyridyl-2-methyl-amino]-acetic acid) as bifunctional chelating agents for labeling biomolecules. These
ligands reacted with the precursor fac-[188Re(CO)3(H2O)3]+ and yielded the radioactive complexes fac-[188Re(CO)3L] (L = three ligands), which were identified by RP-HPLC. The corresponding stable rhenium tricarbonyl complexes (1–3) were
allowed for macroscopic identification of the radiochemical compounds. 188Re tricarbonyl complexes, with log P
o/w
values ranging from −1.36 to −0.32, were obtained with yields of ≥90% using ligand concentrations within the 10−6−10−4M range. Challenge studies with cysteine and histidine revealed the high stability properties of these radioactive complexes,
and biodistribution studies in normal mice indicated a fast rate of blood clearance and high rate of total radioactivity excretion,
primarily through the renal-urinary pathway. In summary, these asymmetric and symmetric pyridyl-containing tridentate ligands
are potent bifunctional chelators for the future biomolecules labeling of fac-[188Re(CO)3(H2O)3]+. 相似文献
3.
Matthias Weil Franz Werner Frank Kubel 《Monatshefte für Chemie / Chemical Monthly》2002,133(3):267-275
Summary. Single crystals of MgAl2F8(H2O)2 have been obtained under hydrothermal conditions (250°C, 14 d) from a starting mixture of AlF3 and MgAlF5(H2O)2 in a 5% (w/w) HF solution. The crystal structure has been determined and refined from single crystal data (Fmmm (#69), Z = 4, a = 7.2691(7), b = 7.0954(16), c = 12.452(2) ?, 281 structure factors, 27 parameters, R(F
2 > 2σ (F
2)) = 0.0282, wR(F
2 all) = 0.0885). The obtained crystals were systematically twinned according to (010/100/001) as twinning matrix, reflecting
the pseudo-tetragonal metric. The crystal structure is composed of perowskite-type layers built of corner sharing AlF6 octahedra with an overall composition of AlF4
− which are connected via common fluorine atoms of [MgF4/2(H2O)2/1] octahedra. Group-subgroup relations of MgAl2F8(H2O)2 to WO3(H2O)0.33 and to other M(II)M(III)2 F8(H2O)2 structures are briefly discussed. Above 570°C, MgAl2F8(H2O)2 decomposes under elimination of water into α-AlF3, β-AlF3, and MgF2.
Received October 29, 2001. Accepted (revised) December 6, 2001 相似文献