An Automated Approach to Luminescence Lifetime and Intensity Titrations

An apparatus is described for the automated collection of luminescence emission decay curves over a wide range of analyte concentrations. The decay curves allow for determination of the excited-state lifetime or calculated steady-state intensity of a luminophore as a function of the analyte concentration. The data presented here demonstrate the use of the apparatus for pH titrations.     

Steady-State and Frequency-Domain Lifetime Measurements of an Activated Molecular Imprinted Polymer Imprinted to Dipicolinic Acid

We recently demonstrated the synthesis and fluorescence activity associated with an optical detector incorporating a molecular imprinted polymer (MIP). Steady-state and time-resolved (lifetime) fluorescence measurements were used to characterize the binding activity associated with MIP microparticles imprinted to dipicolinic acid (DPA). DPA is a unique biomarker associated with the sporulation phase of endospore-forming bacteria. Vinylic monomers were polymerized in a dimethylformamide solution containing DPA as a template. The resulting MIP was then pulverized and sorted into small microscale particles. Tests were conducted on replicate samples of biologically active cultures representing both vegetative stationary phase and sporulation phase of Bacillus subtilis in standard media. Samplers were adapted incorporating the MIP particles within a dialyzer cartridge (500 MW). The permeability of the dialyzer membrane permitted diffusion of lighter molecular weight constituents from microbial media effluents to enter the dialyzer chamber and come in contact with the MIP. Results showed dramatic (10-fold over background) steady-state fluorescence changes (as a function of excitation, emission and intensity) for samples associated with high endospore biomass (DPA), and a frequency-domain lifetime of 5.3 ns for the MIP-DPA complex.     

Hydrostatic high pressure applied to food sterilization III: Application to spices and spice mixtures

Abstract

The response of suspensions of spices and spice mixtures in water to high pressure treatment was investigated. Inactivation of the microbial load–mainly aerobic and unaerobic spore formers–was strongly dependent on water activity and temperature. Samples were completely decontaminated after three pressure cycles (30 min at 80 MPa followed by 30 min at 350 MPa) at 70 °C at a minimum water activity of 0.91. Pressure treated samples were examined for sensory and chemical changes. No significant changes in odour and appearance were recognized by a trained sensory panel, nor were changes in the volatile compounds of the samples detected by static headspace gaschromatography.     

碳化硅色心高压量子精密测量

高压下的量子精密测量对于研究极端环境下的物质结构及演化具有重要意义。针对传统方法难以实现高压下原位高分辨率磁探测的难题,近年来提出了基于固态色心的高压量子精密测量技术,并取得了一系列重要进展,对于推动极端条件下的物质研究具有重要意义。本文主要聚焦基于碳化硅色心的高压量子精密测量,回顾了碳化硅中硅空位色心和双空位色心在高压下的光学和自旋性质,介绍了利用碳化硅色心光探测磁共振技术进行的高压量子传感,包括Nd₂Fe₁₄B的磁性相变、YBa₂Cu₃O_6.6超导体的超导转变温度-压力相图等,展示了基于碳化硅色心的高压量子精密测量在压力传感、压致磁性相变以及压致超导转变方面的应用。

     

Electro-optical granulometer for measurements of flowing particles

An electro-optical technique is described for the measurement of the size distribution of particles from 2.5 μm upwards, in a flow. The apparatus utilizes a white light source, a photodiode array and particle-sizing electronics. The data acquisition system is a multichannel analyser or a microcomputer. This device is an extension to the flow case of a previous device designed for static size analysis, and a critical comparison with size acquisition systems commercially available is given. Shape of particles and outline of waveform during particle detection are discussed.     

二维及三维流场的光学测量方法

对于复杂的非定常流动 ,流场的测量往往要求无干扰、非接触 ,并且能够瞬时记录流场的二维甚至三维信息。对近年来流场测量领域发展快速、应用广泛的几种光学测量方法 ,如 PIV技术及其由此发展而来的 DPIV和 HPV技术 ,做一些介绍和比较。     

Non-contact blood flow determination using a laser speckle method

An electro-optical device is described which allows the non-contact determination of the skin blood flow and its temporal course. As the laser light penetrates the skin, it is not only scattered from the epidermis but also from the moving red blood cells in the capillaries. The scattered light is time dependent and can be described in terms of the dynamic laser speckle effect. Measurements at the skin demonstrate that there is a so-called ‘involuntary body movement’ which must be taken into account when the measurement of the blood flow is determined. Theoretical considerations show a way to reduce the influence of this movement. Some measurements demonstrate the response of the device to blood flow variations.     

粒子图象测速技术在内燃机缸内流场研究中的应用

本文应用粒子图象测速技术（PIV）对内燃机压缩过程中的缸内流场进行了实验研究。开发的实验装置可真实模拟内燃机的进气、压缩和膨胀行程，并且适于开设大窗口以测取大区域的全场速度分布。开发建立的PIV拍摄及数据处理系统实现了对瞬态全场流动参数的较精确的测量处理，可直观地给出流场中的涡流特征。文中有部分结果实例。     

A long-wavelength biolabeling reagent based on the oxonol fluorophore

A red fluorescent dye of the oxonol class, bis-[1-(carboxymethyl)barbituric acid-(5)]-pentamethinoxonol, has been synthesized and, in the form of the succinimidyl active ester, has been applied to antibody labeling for application to flow cytometry and to imaging of tissue sections. The new dye, named CMOX (for carboxymethyloxonol), shows maximum excitation at 583 nm and emission at 611 nm, with a quantum yield of 0.2 in aqueous buffer and methanol. Antibodies labeled with the new dye show favorable brightness, photostability, and low levels of nonspecific binding.     

二维气动探针非定常流测量中动态效应的数值研究

本文采用数值方法模拟了一个三孔探头周围与测压孔内的二维可压缩非定常流场,研究了动态效应对探头绕流和测孔压力的影响,以及相应的测量误差。结果显示,当流场高频振荡时（如： ２０００ Ｈｚ）,其有效测量范围内的速度误差为 ２～４％,气流角误差约为 １０％之内。当流动接近失速时,该项误差迅速增大。文中还给出了高频振荡流中测压孔内外非定常与准定常流场分布之间的差异。     

放气系统流场的光学干涉测量

对于复杂的非定常流动,流场的测量往往要求无干扰、非接触,并且能够瞬时记录流场的信息。采用光干涉方法对放气系统流场的气压变化进行了测量。在加压法测量空气折射率的迈克尔逊双光束干涉实验装置中,引入特制激光功率计和孔状光阑,记录在自由放气过程中等倾条纹中心强度变化与时间的关系曲线,通过折射率与压强和质量流之间的对应关系,进行流场流速的无干扰、非接触的瞬时测量。实验证明这种测量方法响应度好,准确度高。     

A new method for the demodulation of ultrasonic signals for cross-correlation flowmeters

Cross-correlation flowmeters using clamp-on ultrasonic transducers are particularly attractive for use in hostile or abrasive fluids. However, a phase difference is frequently found to exist between the envelopes of the received ultrasonic signals, which causes the correlation peak to become degraded, distorted, or even inverted. In this paper, an explanation for the phase errors is offered, and a new demodulation method, which eliminates these errors, is described.     

Der Einfluß der Detektorkollimierung auf die gemessene mittlere Verweilzeit bei Verweilzeitmessungen mit radioaktiven Indikatoren

Bei der Durchführung von Verweilzeitmessungen mit radikoaktiven Indikatoren wird im Interesse eines hohen Meβeffektes der Detektor nur wenig kollimiert. Dadurch ist der Verlauf der gemessenen Impulsidchte nicht mehr dem Konzentrationsverlauf des radioaktiven Indikators proportional. Es läβt sich jedoch zeigen, daβ die berechnete mittlere Verweilzeit bei symmetrischer Meβeometrie unabhängig vom Einfluβ der Detektrokollimierung ist. Das 2. Moment der Konzentrationsverteilung kann durch entsprechende Korrekturen richtig aus dem gemessenen Impulsdichteverlauf bestimmt werden.     

Evaluating practical measurements of fire-induced vent flows with stereoscopic PIV

Stereoscopic particle image velocimetry (SPIV) was applied to a fire-induced doorway flow to provide the velocity field for computations of the mass flow rate of air into an enclosure. For a flow of uniform temperature and concentration, the technique met all of the requirements to provide the best estimate of the mass flow rate. Simultaneous measurements of vertical distributions of velocity and temperature were also conducted with conventional vent flow techniques, bi-directional probes and thermocouples. Correction factors for mass flow rate computations using the conventional techniques were determined based on comparisons to the SPIV results. An average correction factor of unity was determined for the bi-directional probe technique thus further confirming the utilization of velocity distributions acquired using the technique in mass flow rate computations. An average correction factor of 0.69 was determined for mass flow rates computed from vertical temperature distributions inside and outside the enclosure. This agrees with average correction factors determined in previous studies. The results from the present study suggest that the conventional techniques, which are practical and affordable for routine fire testing, may be applied with greater confidence for fires up to 500 kW.     

Fluorescence Polarization as a Functional Parameter in Monitoring Living Cells: Theory and Practice

The use of fluorescence polarization as a functional parameter in monitoring cellular activation calls for the reliable and accurate measurement of the fluorescence intensity and polarization (FI and FP) of microscopic objects. The relevant experimental parameters that enter such measurements are thoroughly discussed. The possibility of executing FP measurements properly by flow-through systems is compared with that of static cytometry. Remarks on the effects of high-power excitation on markers and cells conclude the paper.     

气液两相流速度及粒径分布激光干涉测量方法的研究

为了实现对气液两相流的粒子粒径、空间分布及其速度测量。对激光干涉气液两相流测量技术（ILIDS）进行了深入研究,该技术是一种应州于气液两相流测量的新技术,其主要优点是不干扰流场和颗粒粒径、位置测量精度高。基于该技术所开发的图像自动处理方法可以利用普通粒子成像测量技术系统拍摄气液两相流的激光散射干涉图像。并利用图像卷积定位、傅里叶变换频率分析及其图像互相关测速等图像处理手段从干涉图像中自动提取粒子的位置、直径和速度信息。为了验证该方法的测量精度,对喷嘴生成的气水两相流进行了测量实验,得到了喷嘴出口处不同区域的粒径、速度矢量的空间分布,并将测得的速度矢量与用粒子成像测量技术方法测得的结果进行对比,证明两种方法测量的平均速度差别仅为0．38％。     

科里奥利力在流量测量中的应用

本文阐述了如何利用科里奥利力精确测量管道中介质的质量流量的原理和方法，并描述了利用科里奥利力的质量流量计的类型和典型结构及其发展情况．