Simulation of induced radioactivity for a heavy ion medical machine

The radioactivity induced by carbon ions of the Heavy Ion Medical Machine (HIMM) was studied to asses its radiation protection and environmental impact. Radionuclides in the accelerator component, and in the cooling water and air at the target area, which are induced from primary beam and secondary particles, are simulated by FLUKA Monte Carlo code. It is found that radioactivity in the cooling water and air is not very important at the required beam intensity and energy that is needed for treatment, while radionuclides in the accelerator component may cause some problems for maintenance work and, therefore, a suitable cooling time is needed after the machine is shut down.     

HIAF BRing及高能外靶实验终端的辐射屏蔽设计

强流重离子加速器（HIAF）是中国科学院近代物理研究所自主研制的一台高能强流重离子加速器,它可以实现p到U的全离子加速。为了保证HIAF运行时的辐射安全,针对该装置的增强器（BRing）及高能外靶实验终端,利用蒙特卡洛程序FLUKA及外推法计算得到了加速p,C及U三种离子时所需的辐射屏蔽。结果表明,加速质子时所需屏蔽厚度最大,并以此为依据给出了全地下结构的屏蔽设计。在此基础上,提出了一种估算高能质子/重离子加速器束流均匀损失时横向屏蔽厚度的方法。结果显示,估算结果与FLUKA计算结果符合较好,验证了该方法的有效性和准确性。High Intensity heavy-ion Accelerator Facility (HIAF) is designed by the Institute of Modern Physics, Chinese Academy of Sciences, which can accelerate particles from proton up to uranium. To guarantee the radiation safety of HIAF during operation, the FLUKA code and extrapolation method were adopted to calculate the shielding thickness. The calculations were based on proton, carbon and uranium particles when losing on the Booster Ring (BRing) and the high-energy experimental terminal. The results indicate that the shielding thickness required for accelerating protons was the largest. Basing on the results, a method for estimating the lateral shielding of a high-energy proton/heavy-ion accelerator was proposed. A good agreement shows between the estimated results and the FLUKA calculated results, the validity and accuracy of the method were verified.     

基于HIAF的物理研究(英文)

在2010年,中国科学院近代物理研究所向国家发展和改革委员会建议了重大科技基础设施--强流重离子加速器装置（High Intensity Heavy-ion Accelerator Facility,简称HIAF）。经过一系列评估和论证,HIAF于2015年12月被国家发展改革委立项。HIAF建设地址位于广东省惠州市,计划于2018年年底正式开工建造。HIAF由超导直线加速器、同步增强器、高能放射性束流线、储存环谱仪以及若干实验测量装置构成,总投资约为25亿人民币。依托HIAF,我们将拓展核素存在版图,研发先进实验技术和方法,开展前沿物理研究;同时,开展重离子束应用研究,服务国家经济社会发展。简要介绍拟建的加速器系统、实验测量装置以及相关的物理研究计划。The Institute of Modern Physics, Chinese Academy of Sciences, proposed the Major National Science and Technology Infrastructure Facility named as High Intensity Heavy-ion Accelerator Facility (HIAF) in 2010. After a series of assessments charged by the National Development and Reform Commission of China, HIAF was officially approved by China government in December, 2015. HIAF will be constructed in Huizhou, Guangdong Province, and the groundbreaking ceremony of construction is scheduled around the end in the year of 2018. HIAF is composed of a superconducting Linac, a booster ring, a high-energy radioactive beam line, a storage ring, and a number of experiment setups. The total investment of HIAF is about 2.5 billion Chinese Yuan. The major goals for HIAF are to explore the hitherto unknown territories in nuclear chart, to approach the experimental limits, to open new domains of physics researches in experiments, and to develop new ideas and heavy-ion applications beneficial to the societies. In this paper, the accelerator complex of HIAF is briefly introduced, and the experimental setups and associated physics research program are presented.     

Simulation of a modified neutron detector applied in CSNS

We simulate the response of a modified Anderson-Braun rem counter in the energy range from thermal energy to about 10 GeV using the FLUKA code. Also, we simulate the lethargy spectrum of CSNS outside the beam dump. Traditional BF3 tube is replaced by the 3He tube, a layer of 0.6 cm lead is added outside the boron doped plastic attenuator and a sphere configuration is adopted. The simulation result shows that its response is exactly fit to H＊（10） in the neutron energies between 10 keV and approximately 1 GeV, although the monitor slightly underestimates H＊（10） in the energy range from thermal energy to about 10 keV. According to the characteristics of the CSNS, this modified counter increases the neutron energy response by 30% compared with the traditional monitors, and it can be applied in other kinds of stray field rich of high eeergy neutrons.     

Study on induced radioactivity of China Spallation Neutron Source

China Spallation Neutron Source (CSNS) is the first High Energy Intense Proton Accelerator planned to be constructed in China during the State Eleventh Five-Year Plan period, whose induced radioactivity is very important for occupational disease hazard assessment and environmental impact assessment. Adopting the FLUKA code, the authors have constructed a cylinder-tunnel geometric model and a line-source sampling physical model, deduced proper formulas to calculate air activation, and analyzed various issues with regard to the activation of different tunnel parts. The results show that the environmental impact resulting from induced activation is negligible, whereas the residual radiation in the tunnels has a great influence on maintenance personnel, so strict measures should be adopted.     

γ辐照后硼硅酸盐玻璃的吸收光谱研究

玻璃固化体是用来固化放射性废物的硅酸盐。作为放射性废物处理的第一道工程屏障,它的耐辐照性能尤其引人注目。本工作使用硼硅酸盐玻璃模拟玻璃固化体材料,用不同剂量的γ射线辐照硼硅酸盐玻璃模拟天然放射性对固化体的辐照损伤。通过测量和分析辐照后硼硅酸盐玻璃的吸收光谱,证实了辐照后在玻璃中产生了E'缺陷,非桥氧空位色心、过氧自由基以及过桥氧联接等缺陷。此外,还得出了不同微观结构随吸收剂量的演化关系。对于辐照后产生的缺陷,它们的浓度都随吸收剂量的增加而增大。同时,发现玻璃在辐照后其吸收光谱的带隙随着剂量的增加而逐渐变窄;而当吸收剂量大于等于105 Gy时,玻璃的带隙则达到饱和值。Vitrification is one kind of silicates which is used for immobilization of high-level waste (HLW). As the first engineered barrier of HLW disposition, its anti-irradiation characteristic is particularly noticeable. Vitrification is replaced by borosilicate glass to investigate radiation effect, and the irradiation damage generated by natural radioactivity in vitrification is simulated by different doses gamma rays on borosilicate glass. By measuring and analyzing the absorption spectrum of irradiated borosilicate glasses, it is confirmed that E'defect, non-bridging oxygen hole center, peroxy dangling bond and bridge oxygen link, etc. are induced in borosilicate glass after irradiation. Furthermore, the relations between the defects and absorbed doses are shown. For the concentrations of these defects induced by irradiation, all of them increase with absorbed dose. Meanwhile, absorption band gap in borosilicate glass after irradiation decreases with absorbed dose and the band gap becomes saturated when absorbed dose is equal to or greater than 105 Gy.     

关于在HIAF-BRing上开展自旋物理研究的提议(英文)

国家重大科技基础设施"强流重离子加速器装置"（High Intensity heavy-ion Accelerator Facility,HIAF）已由国家发改委批准立项并开始建设。建成之后,HIAF将为微观物质结构和重离子应用等研究提供很好的实验平台。HIAF的加速储存环（Booster Ring,BRing）设计可以加速最高动量为11.9 GeV/c的高流强质子束流。因此,HIAF-BRing将为GeV能区的核物理和强子物理研究带来新的机遇。另一方面,极化实验是研究微观物质及其相互作用的有力工具。我们提议启动相关物理和极化技术的预研工作,为在HIAF-BRing上开展自旋物理研究打下基础。The construction of the future scientific facility High Intensity heavy-ion Accelerator Facility (HIAF) in China has started. Once established, HIAF will provide excellent conditions for fundamental investigations on both matter structure and heavy-ion applications. The booster ring (BRing) of HIAF is designed to accelerate high-intensity protons with the maximum momentum of 11.9 GeV/c. Therefore it will bring new opportunities for the nuclear and hadron physics in the GeV region. Polarized experiments have been proved as a powerful tool in the explorations of the building blocks of matter. We propose to initiate a pre-investigation for the related physics and polarization techniques, which will lay the foundation of the spin physics at the HIAF-BRing.     

利用HIRFL高能重离子束的核能材料辐照损伤研究

载能重离子与高能中子在材料中能够产生相似的级联碰撞损伤,加之重离子具有大的离位损伤截面和在材料样品中低的感生放射性,载能重离子束成为模拟先进核能装置内部结构材料辐照损伤的重要手段。HIRFL能区的重离子在结构材料中的射程一般远大于晶粒尺寸,因此能够产生材料体损伤,借助小样品技术可以获得材料力学性能变化（尤其辐照脆化）的有用信息,为探讨材料辐照损伤微结构和宏观力学性能变化的关联提供了重要条件。本文简要介绍了近年来我们基于HIRFL高能离子束开展的聚变堆候选材料辐照损伤的研究,包括低活化钢的辐照脆化行为、氧化物弥散强化（ODS）铁素体钢的结构优化对于抗辐照性能的影响、不同载能粒子辐照条件下铁素体/马氏体钢的辐照肿胀数据的关联,以及高能重离子辐照的钨材料中氢同位素的滞留行为。研究表明,结合特殊的测试技术及数据分析方法,高能重离子可作为核能结构材料辐照损伤研究及评估的有效手段。Because of the similarity in cascade damage structure in materials produced by energetic heavy ions and by fast neutrons, and the high displacement rate and low induced radioactivity of samples by heavy ions, heavy ion beam becomes an important tool to simulate radiation damage by energetic neutrons in materials in advanced nuclear energy systems. The ranges of heavy ions provided by HIRFL (Heavy Ion Research Facility in Lanzhou) are generally much larger than the mean dimensions of grains in alloys candidate to advanced nuclear reactors, and is capable of producing radiation damage in bulk scale. It therefore makes possible the evaluation of change of mechanical properties including the radiation induced embrittlement from the irradiated specimens by using miniaturized specimen techniques. In the present paper, we provide an introduction of our recent studies of radiation damage of materials candidate to future fusion reactors by utilizing heavy ion beams in HIRFL.The studies include issues as follows:ductility loss of RAFM steels causes by high-energy Ne ions, impact of oxide dispersoids on the radiation resistance of ODS ferritic steels, correlation of void swelling of ferritic/martensitic steels under different particle irradiation, and behavior of deuterium retention in tungsten under irradiation with high-energy heavy ions. The results show that high-energy heavy ions can be used as a tool to efficiently investigate or evaluate radiation damage in structure materials if combined with some special test techniques and data analysis.     

核爆炸产物放射性衰变过程的研究

核爆炸后产物放射性和毒性及其随时间的变化是有关安全保障和环境保护的主要问题．利用新开发的数据库和程序对该问题进行了详细的理论研究,给出了裂变产物的放射性活度、生物潜在危害因子和能量沉积等量的演化情况,列出了主要放射性核的贡献,还分析了由于诱导反应在土壤和岩石中产生的放射性同位素的变化. The evolution of radioactivity and poisonousness of the products after nuclear explosion is very important for safety and environmental protection. The detailed study of the evolution of radioactivity of fission products, biological hazard potential, energy deposition and so on was performed by using the new data base and code. The contributions of the main radioactive nuclei and the evolution of radioisotopes in soil and rock are also discussed.     

高能重离子辐照的ODS铁素体钢脆化效应研究

与传统的铁素体钢相比,氧化物弥散强化(ODS) 的铁素体钢具有更优的耐高温和抗辐照性能,近年来成为先进核能装置重要的候选结构材料。在HIRFL 的扇聚焦型回旋加速器(SFC) 材料辐照终端,对一种氧化物弥散强化(ODS) 铁素体钢MA956 进行了高能Ne 离子辐照实验,旨在研究级联碰撞损伤和惰性气体原子注入条件下该材料力学性能的变化。利用辐照终端的能量衰减装置将SFC出口123.4 MeV的离子能量分解为介于38.5~121.0 MeV之间的30 个入射能量值,并通过双面辐照在厚度60 μm的样品中均匀产生了损伤。辐照剂量为9x1016 ions/cm2,在样品中的平均位移损伤为0.7 dpa,注入的Ne原子浓度为350 appm。辐照期间样品温度保持在440 ℃附近。对辐照前后的样品分别在室温和500 ℃下进行了小冲杆试验(Small-punchTest),获得了辐照前后样品的加载位移曲线,由此得到该辐照条件下样品的延性损失为18%~26%。通过扫描电子显微镜观察了断口形貌和厚度变化,估算了样品的等效断裂应变和断裂韧性。结果表明,MA956 钢经过高能Ne离子辐照后等延伸率减小,断裂韧性降低,样品发生了一定的脆化。透射电镜结果说明氧化物弥散相界面处微空洞的形成可能是导致脆化的原因。Oxide dispersion strengthened (ODS) ferritic steels have better high-temperature creep rupture strength and higher irradiation resistance than conventional ferritic steels, and show high prominence of application in advance nuclear reactors. Their stability under high-dose radiation conditions needs to be clarified. In the present study, a commercial ODS ferritic steel MA956 were irradiated with high 20Ne ions at a terminal chamber of the Sector-focused Cyclotron (SFC) at HIRFL (Heavy-ion Research Facility in Lanzhou). With the energy gradient degrader of the irradiation chamber, the primary energy (123.4 MeV) of the Ne-ion was dispersed into 30 different energies between 38.5~ 121.0 MeV, which resulted in a plateau distribution of lattice damage in the specimens. The specimens were irradiated from both sides so that the whole 60 m thickness was nearly uniformly damaged. The specimen temperature was maintained around 440 ℃ during the irradiation. The irradiation dose is about 9x1016 ions/cm2, corresponding to a damage level of 0.7 dpa and a Ne concentration of 350 appm. The specimens before and after irradiation were tested with the Small-punch Test technique, at room temperature and 500 ℃, respectively. The fracture morphology was observed by scanning electron microscopy.The results show that MA956 underwent some loss of ductility and fracture toughness after the irradiation with high-energy 20Ne ions. It may be ascribed to the formation of nano-scale cavities at the oxides/matrix interfacesin the ODS steel specimens under irradiation .     

The two-neutron transfer reactions (O, O) with O, C and Mg targets

Excitation functions, angular distributions and differential ranges were measured for the ²⁶Mg(¹⁸O, ¹⁶O)²⁸Mg reaction at ¹⁸O beam energies of 20–45 MeV. Excitation functions only were measured for the reactions ¹⁴C(¹⁸O, ¹⁹O)¹³C, ¹⁴C(¹⁸O, ¹⁶O)¹⁶C, ¹⁴C(¹⁸O, ²⁰O)¹²C, ¹⁴C(¹⁸O, ¹⁵N)¹⁷N and ¹⁸O(¹⁸O, ¹⁹O)¹⁷O, ¹⁸O(¹⁸O, ¹⁶O)²⁰O, ¹⁸O(¹⁸O, ¹⁵N)²¹F at ¹⁸O beam energies of 13–41 MeV. We have identified these as direct reactions in which a single neutron, a two-neutron cluster, a deuteron and a triton are transferred between projectile and target.

The cross sections for two-neutron transfer reactions were found to be relatively high and those for the ¹⁸O+¹⁸O and the ¹⁴C+¹⁸O reactions were higher than the ones of single-neutron transfers over most of the energy range.

Attempts were made to apply the theory of Buttle and Goldfarb for single-neutron transfer to the case of two-neutron transfer in the ²⁶Mg(¹⁸O, ¹⁶O)²⁸Mg reaction below the Coulomb barrier. It is shown that for those reactions for which the assumptions, implicit in the model, are valid, good agreement is obtained with experiment. We also tried to apply the diffraction model of Dar and Kozlovsky to the calculation of the angular distribution of these reactions. A good fit to the experimental results could be obtained if quite different sets of parameters were used in the calculations for the two bombarding energies.     

Radiation effects of electrons and protons on the ceramic oxide superconductor YBaCuO

The irradiation of the high T_c superconducting material YBaCuO has been carried out by using 200 keV proton, and 400 keV and 8 MeV electron beams. The temperature of zero resistance increases from 86.7 to 89.8 K with proton implantation while 8 MeV electron irradiation reduces the zero resistance temperature by 3 K with an irradiation dose of 2.25×10¹⁴e^-/cm². However, wich an irradiation dose of 1.35×10¹⁵e^-/cm² the 8 MeV electron beam can make the superconductor become insulating. The in situ examination of a high resolution transmission electron microscope has proved that the amorphous region in the system has ordered arrangement whereas the crystalline region turns disordered under 400 keV electron irradiation with very high doses up to 10²⁶ e^-/cm². The experiments demostrate that proton and electron irradiations exhibit quite different effects both in its structure and property.     

利用18O(6Li,d)22Ne反应研究22Ne Eα=470 keV共振态的性质

慢速中子俘获过程（s过程）是合成比铁重元素的重要途径之一。22Ne（α,n）25Mg反应是大质量AGB星中s过程主要的中子源,其中的22Ne主要通过14N（α,γ）18F（β+）18O（α,γ）22Ne反应链合成。该反应链中关键反应18O（α,γ）22Ne在天体物理感兴趣能区的截面非常低,其天体反应率主要来自于22Ne α分离阈附近低能共振态的贡献,但目前相关能级的共振参数严重缺失。在HI-13串列加速器的Q3D磁谱仪上,通过测量18O（6Li,d）22Ne反应的角分布,利用DWBA分析确定了22Ne分离阈附近共振能级E_α=470 keV的自旋宇称为0+,为后续计算18O（α,γ）22Ne的天体反应率打下了基础。About a half of the abundances of elements heavier than iron comes from the so-called slowneutron capture process (s-process) in Asymptotic Giant Branch (AGB) stars, with the 22Ne(α, n)25Mg reaction as one of the main neutron sources. In the beginning phase of AGB thermal pulse, 22Ne is produced by the 14N(α, γ)18F(β+)18O(α, γ)22Ne reaction sequence, in which the 18O(α, γ)22Ne reaction plays a key role. While the reaction rate of the 18O(α, γ)22Ne is mainly affected by several resonant states lying closely to the α threshold in 22Ne, up to now, the relevant 22Ne parameters are fragmentary in the energy region corresponding to the typical temperatures of s-process. The direct measurement of the 18O(α, γ)22Ne reaction rate is extremely difficult due to the very low cross section. In this work, we investigated the 22Ne resonant states via the 18O(6Li, d)22Ne reaction at the Beijing HI-13 tandem accelerator of China Institute of Atomic Energy. Based on the DWBA analysis, preliminary results showed that the spin-parity of 22Ne E_α=470 keV resonant states was assigned as 0+, which would make contributions to subsequent calculation for the reaction rate of the 18O(α, γ)22Ne.     

退火对Pb辐照Al2O3单晶发光特性的影响

研究了230MeV的²⁰⁸Pb²⁷⁺辐照Al₂O₃样品及随后在600,900,1100K高温条件下退火后的光致发光特性。从辐照样品的测试结果可以清楚地看到在波长为390,450nm处出现了强的发光峰。辐照量为1×10¹³ions/cm²时,样品的发光峰最强。经过600K退火2h后测试结果显示,380nm发光峰剧烈增强,而其他发光峰显示不明显。在900K退火条件下,380nm的发光峰开始减弱,而在360,510nm出现了明显的发光峰,至到1100K退火完毕后380nm的发光峰完全消失,而360,510nm的发光峰相对增强。从被辐照样品的FTIR谱中看到,波数在460~510cm^-1间的吸收是振动模式,经过离子辐照后,吸收带展宽,随着辐照量的增大,Al₂O₃振动吸收峰消失,说明Al₂O₃振动模式被完全破坏。1000~1300cm^-1之间为Al—O—Al桥氧的伸缩振动模式,辐照后吸收带向高波数方向移动,说明其振动模式受到影响。辐照剂量较小的样品,损伤程度相对较低,经退火晶化后,振动模式基本恢复到单晶状态;辐照剂量较高的样品,损伤程度大,退火处理后表面变得较粗糙,振动模式并未出现,说明结构破坏严重。     

Modification of optical, chemical and structural response of CR-39 polymer by 50 MeV lithium ion irradiation

CR-39 polymer samples were irradiated with 50 MeV lithium ion beam; the fluence was varied in the range 10¹¹–10¹³ ionscm⁻². Irradiation effects were studied using UV–visible, FTIR spectroscopic and X-ray diffraction techniques. The observation of the recorded spectra shows that the detector is sensitive to swift ions irradiation and its UV absorption is influenced by the stopping power (dE/dx)_e. The FTIR spectra does not show any considerable changes due to the irradiation indicating that the detector is chemically stable. No appreciable change in the diffraction pattern of CR-39 polymer after irradiation upto the fluence level of 10¹³ ionscm⁻² is observed, showing its structural stability also.     

Dosimetry of 40 and 70 MeV/n O beams

Using CR-39 plastic track detectors the range values of ¹⁶O ions at two different energies (initially in the beam line, 39.97 MeV/n and 69.98 MeV/n) were measured after escaping the beam pipe and found to be (3050 ± 40) μm and (8210 ± 90) μm, respectively. The longitudinal and projected angular spread of oxygen ions of an initial energy of 69.98 MeV/n in the region of the Bragg peak was derived from the measured geometrical parameters of tracks. Based on a calibration curve (etch rate ratio vs total linear energy transfer in CR-39) and the measured track length distribution at the range end of oxygen ions, the complete depth dose profile of a 67.7 MeV/n ¹⁶O beam in CR-39 (plateau, extended Bragg peak and residual ionization caused by projectile like fragments) was obtained.     

Shell-model calculations with Woods-Saxon wave functions in O and Ca

Two-particle states of ¹⁸O and ⁴²Ca are calculated within the shell model. The nuclear cores are represented by Woods-Saxon potentials, and for the residual interaction, central forces of Yukawa and Gaussian types are used. The wave functions for the low-lying states except for the O₂⁺ and 2₂⁺ states in ⁴²Ca are obtained, and the calculated energy spectra are in good agreement with experiments. Also E2 transitions are calculated and compared with experimental results.     

恒星氦燃烧关键反应12C(α,γ)16O天体物理S因子及其反应率

恒星氦燃烧阶段3α反应和12C（α,γ）16O反应相互竞争,两者的反应率共同决定了氦燃烧结束后12C与16O的丰度比,该比值是大质量恒星后继演化以及伴随的元素核合成过程的初始条件。目前,氦燃烧12C（α,γ）16O反应起始T₉=0.2处,天体物理模型要求的反应率的精确度要低于10%,然而尚未有实验或理论给出满足要求的结果。最为直接和可靠地获取12C（α,γ）16O反应率的方法,就是尽可能往低能区测量其天体物理S因子,然后通过理论外推到感兴趣的能区。为此基于经典的R-矩阵理论,建立了适用于低能核反应的多道、多能级的约化R-矩阵理论来拟合几乎所有可用的16O系统的实验数据。配合使用协方差统计和误差传播理论,拟合外推得到了客观的、内部自恰的和唯一性好的12C（α,γ）16O反应天体物理S因子。总的外推S因子STOT（0.3 MeV）=162.7±7.3 keV·b,理论上首次给出达到恒星演化与元素核合成模型的最低要求的S因子。基于计算给出的全能区的S因子,数值积分给出了温度位于0.04 6 T₉ 6 10的12C（α,γ）16O天体物理反应率。在T₉=0.2处,推荐的反应率为（7.83 ±0.35）×10-15 cm3mol-1s-1。During stellar helium burning, the rates of 3α and the 12C(α,γ)16O reaction, in competition with one another, determine the relative abundances of 12C and 16O in a massive star. The abundance ratio is the beginning condition of the following nucleosynthesis and star evolution of massive stars, which are extremely sensitive to the rate of 12C(α,γ)16O reaction at T₉=0.2. The most direct and trustworthy way to obtain the reaction rate of the 12C(α,γ)16O reaction is to measure the S factor for that reaction to as low energy as possible, and to extrapolate to energies of astrophysical interest. Based on a new multilevel and multichannel reduced R-matrix theory for applications in nuclear astrophysics, we have obtained an accurate and self-consistent astrophysical S factor of 12C(α,γ)16O, by a global fitting for almost all available experimental data of 16O system, with the coordination of covariance statistics and error-propagation theory. The extrapolated S factor of 12C(α,γ)16O was obtained with a recommended value STOT (0.3 MeV)=162.7±7.3 keV·b. And the reaction rates of 12C(α,γ)16O for stellar temperatures between 0.04 6 T₉ 6 10 are provided. At T₉=0.2, the reaction rate is (7.83 ±0.35)×10-15 cm3mol-1s-1, where stellar helium burning occurs.     

Free radicals inhibitor effect on makrofol E nuclear tracks. Preliminary data

Thin foils of Makrofol E prepared with and without inclusion of a free radical scavenger (DPPH), were irradiated at the Tandar facilities. 374 keV and 50 keV ¹⁶O⁺⁺ ions from a Varian Ion Implanter and ⁷Li⁺ ions at different energies from a Tandem accelerator were used. Replicas of the etched foils were observed by transmission electron microscopy.

Track 3iameters exhibited a slight difference between the treated and untreated foils in the case of irradiation with ¹⁶O ions. A larger difference between the groups in the case of ⁷Li ions was found.     

羧甲基-β-1,3-葡聚糖对人肝癌HepG2细胞的放射增敏作用

本研究旨在探讨羧甲基-β-1,3葡聚糖（CMG）对人肝癌HepG2细胞X射线或12C6+离子束辐射敏感性的影响。首先用CCK-8法检测CMG对HepG2细胞的生长抑制情况,得到半数抑制浓度（IC50）为120.6μg/mL。用浓度为0.1×IC50的CMG预处理HepG2细胞24 h,再给予2 Gy X射线或12C6+离子束辐照（CMG+辐照组）;CMG未处理组直接接受2 Gy X射线或12C6+离子束辐照（辐照组）。对比分析辐照组和CMG+辐照组细胞的克隆存活、DNA损伤、凋亡与周期分布、细胞内活性氧（ROS）水平。发现:与X射线辐照组相比,相同剂量的12C6+离子辐照组克隆存活率更小,DNA损伤和周期阻滞更加严重,细胞凋亡率和细胞内ROS水平也更高。与单独X射线或12C6+离子束辐照组相比,CMG+辐照组克隆存活率明显降低,细胞凋亡率随辐照后CMG作用时间的延长而明显增加,CMG使辐照后细胞内ROS维持在一个较高的水平,同时CMG明显加重了单独辐照诱导的DNA损伤和周期阻滞。结果表明,与X射线相比,HepG2细胞对相同剂量的12C6+离子辐射更敏感;CMG可增加HepG2细胞对X射线或12C6+离子辐射的敏感性;CMG可能通过增加受照HepG2细胞内的ROS水平,加剧辐照诱导的DNA损伤,促进辐射诱导细胞凋亡而起到辐射增敏作用。This study aims to investigate the effect of carboxymethy-β-1, 3-glucan (CMG) on the sensitivity of human hepatoma HepG2 cells to X-rays or 12C6+ ions irradiation. First, the inhibitory effect of CMG on the growth of HepG2 cells was detected by CCK-8 assay, and the half maximal inhibitory concentration (IC50) was 120.6 μg/mL. HepG2 cells were pretreated with CMG at a concentration of 0.1×IC50 for 24 h and then irradiated with 2 Gy X-ray or 12C6+ ion beams (CMG + irradiation group). CMG untreated group was directly irradiated by 2 Gy X-rays or 12C6+ ions beam (irradiation group). The clone survival, DNA damage, cell apoptosis, cell cycle distribution, and intracellular reactive oxygen species (ROS) levels in irradiation group and CMG + irradiation group were comparatively analyzed. The results showed that the clone survival rate was lower, DNA damage and cycle arrest were more serious, and the rate of apoptosis and intracellular ROS levels were higher in 12C6+ ions irradiation group than those in the same dose of X-rays irradiation group. Compared with X-rays or 12C6+ ions irradiation group, the clone survival rate of CMG + irradiation group was significantly decreased, and the apoptosis rate significantly increased with the prolongation of CMG treatment post-irradiation; CMG maintained intracellular ROS at a higher level after irradiation, CMG also significantly aggravated radiation-induced DNA damage and cycle arrest. These results indicated that HepG2 cells were more sensitive to 12C6+ ions radiation than those at the same dose of X-rays. CMG increased the sensitivity of HepG2 cells to X-rays or 12C6+ ions irradiation by increasing intracellular ROS level, exacerbating radiation-induced DNA damage and promoting radiation-induced apoptosis in irradiated HepG2 cells.