Phospholipid-stabilized microbubbles: Influence of shell chemistry on cavitation threshold and binding to giant uni-lamellar vesicles

We demonstrate the feasibility of covalently linking a single microbubble to a single, giant uni-lamellar vesicle (GUV). Such a combination of GUV plus microbubble might prove useful as a new drug delivery vehicle involving microbubble cavitation-induced sonoporation of the vesicle bilayer as a release mechanism. We therefore applied the well known methodology of passive cavitation detection to measure the influence of lipid shell chemistry on inertial cavitation thresholds for externally added microbubbles. We find that cavitation threshold changes significantly with changes in either molecular weight or mole fraction of poly(ethylene glycol), historically used to impede gas dissolution and microbubble coalescence. We attribute changes in cavitation threshold to changes in microbubble resonance frequency resulting from changes in microbubble shell bending elasticity. To further demonstrate the influence of shell chemistry on microbubble behavior, we describe how several common bubble phenomena - and some new - respond to changes in lipid chain length.     

Treatment-related central nervous system toxicity: MR imaging evaluation with CT and clinical correlation

Thirteen patients with abnormal brain MR scans attributable to treatment-induced injury were retrospectively reviewed. All patients were treated with radiation therapy and 62% received chemotherapy. Five patients were graded as having severe white matter (WM) changes, four had moderate WM changes, and four had mild WM changes. CT was generally equivalent to MR in evaluation of severe and moderate WM abnormalities, whereas MR was superior to CT in detection of mild WM abnormalities. In general, the severity of changes depicted by MR/CT correlated with the extent of neurologic dysfunction. The most severe changes were seen in those patients treated with combination irradiation and chemotherapy.     

青藏高原东北部闪电M变化多参量观测

利用闪电光通道高速摄像、 地面电场变化和峰值辐射强度同步资料, 对比分析了负地闪连续电流阶段和人工触发闪电放电过程中M变化特征. 结果表明, 通道亮度资料能够辅助地面电场波形来准确判断是否为真实的M过程. 在经典M变化产生前及其过程中会产生很多快速电场变化MP(称为预M变化)变化, 大量MP促成了M变化. MP变化和K变化在脉冲特征上没有太多差别, 产生的物理机制是相似的. MP变化是连续电流阶段快速电场变化, 是击穿过程引起的电荷快速流动的结果, 并伴有通道突然增亮和高强度辐射. M变化脉冲波形多数为单极性, 有正有负, 少数呈现不规则变化, 持续时间在0.1 ms之内.而经典M变化的U 形结构只适用于近闪, 波形是静电场所致, 持续时间约0.2–0.8 ms. MP和K变化都是击穿所致, 只是MP变化有电荷流入原回击通道, 而K变化没有. 关键词： 青藏高原 M变化')" href="#">M变化 MP变化')" href="#">MP变化 K变化')" href="#">K变化     

The aging adult voice

Advancing age produces physiologic changes that may alter voice. Some of these changes are inevitable; others may be avoidable or reversible. In addition, many treatable medical conditions may cause voice changes similar to those of aging. It is essential that all voice care providers be familiar with the expected changes of aging, and be alert to reversible conditions that may adversely affect phonation and be mistaken for presbyphonia.     

埋入式光纤法布里—珀罗温度和应变传感器模型研究

研究了埋入式内置和外置型法布里－珀罗光纤传感器模型，这一框蒋 传感器中应变和温度的变化同反射光强联系起来，其中；子模型Ⅰ建立了传感器内的温度与应变同传感器长度变化及传感器光学性质变化的函数关系；子模型Ⅱ建立了传感器长度和光学性质的变化同反射光强变化的函数关系。     

利用酪氨酸残基的共振瑞利散射研究溶液中蛋白质构象

基于酪氨酸残基K带激发峰在较高浓度的蛋白质溶液中表现出特征散射峰,通过Δλ＝0同步荧光扫描识别,建立用荧光分光光度法测定酪氨残基K带的共振散射峰强度及其波长位移来推测溶液中蛋白质构象变化的新方法。方法不受蛋白质色氨酸残基的干扰,图谱简单明了直观。文章叙述了方法建立的实验基础,并用构建的方法研究了盐效应、酸碱性效应对蛋白质构象变化的影响,最后通过测定不同温度下酶活性的实验验证了方法对温度影响酶蛋白质构象变化的分析的正确性。     

First order phase change detection using planar waveguide Bragg grating refractometer

Solid-to-liquid and gas-to-liquid phase changes in water and ordered-to-isotropic phase changes in a nematic liquid crystal are detected with an optical sensor. A planar Bragg grating defined purely by refractive index modulation is covered with a water or liquid crystal overcladding and the temperature is controlled to trigger phase changes. Measurement of the Bragg wavelength allows changes of effective refractive index to be detected and discontinuities in behaviour caused by phase transitions can be clearly identified.     

A multimodality investigation of cerebral hemodynamics and autoregulation in pharmacological MRI

Pharmacological MRI (phMRI) methods have been widely applied to assess the central hemodynamic response to pharmacological intervention as a surrogate for changes in the underlying neuronal activity. However, many psychoactive drugs can also affect cardiovascular parameters, including arterial blood pressure (BP). Abrupt changes in BP or the anesthetic agents used in preclinical phMRI may impair cerebral blood flow (CBF) autoregulation mechanisms, potentially introducing confounds in the phMRI response. Moreover, relative cerebral blood volume (rCBV), often measured in small-animal phMRI studies, may be sensitive to BP changes even in the presence of intact autoregulation. We applied laser Doppler flowmetry and MRI to measure changes in CBF and microvascular CBV induced by increasing doses of intravenous norepinephrine (NE) challenge in the halothane-anesthetized rat. NE is a potent vasopressor that does not cross the blood-brain barrier and mimics the rapid BP changes typically observed with acute drug challenges. We found that CBF autoregulation was maintained over a BP range of 60-120 mmHg. Under these conditions, no significant central rCBV responses were observed, suggesting that microvascular rCBV changes in response to abrupt changes in perfusion pressure are negligible within the autoregulatory range. Larger BP responses were accompanied by significant changes in both CBV and CBF that might confound the interpretation of phMRI results.     

Laser heterodyne measurements of photo-induced refractive-index changes in amorphous As-S films

A differential-type heterodyne interferometer has been successfully utilized for studying the refractive-index changes in As-S films under illumination. The measuring system can detect phase changes down to 1° in angle in accuracy and sensitivity. It is confirmed that illumination of the films involves two distinct factors: a thermal and an optical one. The index changes are caused only by the optical effect. The time-dependent characteristics of the index changes are explained to be closely related to the photo-darkening effect.     

Sodium and proton diffusion MRI as biomarkers for early therapeutic response in subcutaneous tumors

The ability to quantitate early effects of tumor therapeutic response using noninvasive imaging would have a major impact in clinical oncology. One area of active research interest is the ability to use MR techniques to detect subtle changes in tumor cellular density. In this study, sodium and proton diffusion MRI were compared for their ability to detect early cellular changes in tumors treated with a cytotoxic chemotherapy. Subcutaneous 9L gliosarcomas were treated with a single dose of 1,3-bis(2-chloroethyl)-1-nitrosourea. Both sodium and diffusion imaging modalities were able to detect changes in tumor cellularity as early as 2 days after treatment, which continued to evolve as increased signal intensities reached a maximum approximately 8 days posttreatment. Early changes in tumor sodium and apparent diffusion coefficient values were predictive of subsequent tumor shrinkage, which occurred approximately 10 days later. Overall, therapeutical induced changes in sodium and diffusion values were found to have similar dynamic and spatial changes. These findings suggest that these imaging modalities detected similar early cellular changes after treatment. The results of this study support the continued clinical testing of diffusion MRI for evaluation of early tumor treatment response and demonstrate the complementary insights of sodium MRI for oncology applications.     

Theory of chemisorption of hydrogen on transition metals and their alloys

A theoretical discussion is given of the changes in the heat of chemisorption of a hydrogen atom upon alloying a group-VIII metal with a group-IB metal. The emphasis is on two concepts: the ensemble effect and the ligand effect. The ensemble effect ascribes the changes in the heat of chemisorption primarily to changes in the geometry of the adsorption complex, whereas the ligand effect also takes into account changes in bond strength due to a different intrinsic activity of the bonding metal atoms.     

Hyperthermia induces T1 relaxation and blood flow changes in tumors. A MRI thermometry study in vivo

Regional hyperthermia in combination with chemotherapy and/or radiotherapy has proven to be an effective treatment concept for locally advanced deep-seated tumors. Simultaneous MR-imaging could be a promising approach for therapy optimization. Purpose of this study was the in vivo investigation of temperature induced longitudinal relaxation time (T(1)) and blood flow changes in a tumor model. Using a 1.5 Tesla MR system, the T(1) sensitivity on temperature and the onset of tissue changes at temperatures >44 degrees C were investigated in muscle samples. Also, fourteen Syrian Golden Hamsters with amelanotic melanoma A-MEL-3 were examined during heating of the tumors. Temperature induced blood flow and T(1) changes were determined continuously during hyperthermia. Changes of T(1) correlated linearly with temperature over a wide range (27-44 degrees C) in the tissue sample. Tissue changes became notable above 44 degrees C. In the tumor model, relative changes of T(1) (unlike blood flow) showed linear correlation with temperature over the entire range of hyperthermia relevant temperatures (32-44 degrees C). For a low thermal dose, T(1) allows the assessment of temperature changes in tumors in vivo. At higher thermal doses, in addition to temperature changes, T(1) also shows tissue changes. Both temperature and tissue changes supply important information for hyperthermia.     

On the evaluation of measurements of small changes in electric resistance

The measurement of small changes in resistance, caused in metal wires by non-equilibrium point defects, meets with many difficulties caused inter alia by changes in temperature, instability of the voltage source and irreversible changes in the electric resistance due to the thermal treatment of the samples. In the first part of the paper, dealing with the d-c bridge method of measurement, the conditions under which the relations given in the literature can be used for calculating small changes in resistance are quantitatively determined on the basis of a detailed analysis of such relations. The second part describes a method of measurement permitting the elimination of irreversible changes in the resistance of thermally treated samples and relations are derived for calculating reversible changes.     

Functional phantom for fMRI: a feasibility study

Functional magnetic resonance imaging (fMRI) reveals changes in blood oxygen level-dependent (BOLD) signal after considerable processing. This paper describes the implementation and testing of an fMRI phantom where electric current applied to a thin wire within a proton-rich medium substituted BOLD distortion of the magnetic field; the scanner detects these two distortions as practically identical signal changes. The magnitude of the change depended on the current strength. The phantom has a number of possible applications. Signal changes across sessions, days, instruments and individuals could be monitored. Placing the phantom close to a subject during an fMRI experiment could allow differentiating sensitivity changes in the scanner due to instrumentation from changes in the subject's state and performance during the experiment. The spatial extent of brain activations and effects of various changes in the chain of image formation could be analyzed using current-induced "activations". Furthermore, the phantom could expedite fMRI sequence development by reducing the need to scan human subjects, who introduce uncertainty to the signal. Thus, this fMRI phantom could be useful for both cognitive fMRI studies and scanner calibration.     

热作用过程中生物组织特性变化的光学监测

基于热作用下生物组织特性的理论，描述了最新的理论研究进展，介绍了将光学监测技术用于热致组织特性变化研究的原理与方法，并给出了典型的实验结果；生物组织的散射系数与损伤特性的关联：生物组织的吸收系数对脱水量的依赖性；热作用下血液灌注的光学成像监测。将光电技术用于热作用过程中生物组织特性变化的监测，对进一步完善热疗理论、实现热疗的在体监测具有重要的理论意义与实践意义。     

MULTIPLE DAMAGE LOCATION WITH FLEXIBILITY CURVATURE AND RELATIVE FREQUENCY CHANGE FOR BEAM STRUCTURES

The purpose of this paper is to seek efficient methods for multiple damage location in beam structures. Two methods are studied in terms of finite element model (FEM) simulations on beam structure. Firstly, a finite element model of reinforced concrete beam is established to compute changes in flexibility and flexibility curvature of the beam with various damage patterns, and sensitivities of flexibility and flexibility curvature for closely distributed damage patterns of various extents are also compared. Due to its high sensitivity to closely distributed damages, flexibility curvature is recommended for multiple damage location. Secondly, the full correlation between the relative frequency changes and the analytical frequency changes are confirmed with FEM analyses: therefore, the relative frequency changes, rather than the frequency changes themselves, are used in the multiple damage location assurance criterion (MDLAC) to increase the location efficiency. Numerous examples are presented to verify the two methods.     

PVT data for liquid and solid cyclohexane,cyclohexanone and cyclopentanol up to 3000 bar

PVT data have been determined for cyclohexane (194–338 K), cyclohexanone (214–303 K), and Cyclopentanol (204–325 K) up to 3000 bars, as well as the volume changes accompanying the phase transitions. The volume changes allow the calculation of the corresponding enthalpy and entropy changes on using the Clausius-Clapeyron equation. The volume changes of melting decrease considerably with increasing pressure, whereas those due to solid-solid transitions exhibit much less of a pressure dependence. The PVT data of the liquid phases are described with the Tait equation.     

Recent advances in ultrafast time‐resolved chirality measurements: perspective and outlook

Observing chirality changes as they occur is an important topic of research. It provides information that deepens the understanding of biomolecular configuration and conformation under environmental changes. Also, knowing the specific steps in chiral synthesis would simplify the production of specific chiral enantiomers that have a specific function. To gain better insight to the initial steps of conformational and configurational changes, the time‐resolution of chiral spectroscopy is continually pushed toward a shorter time‐scale. Recent advances have produced measurements of chirality changes with a femtosecond time‐resolution. These measurements are hindered by the inherently weak chirality signal, which can be overshadowed by different optical artefacts. This minireview will look at the so far successful techniques which measure chirality changes with femtosecond time‐resolution and discuss the advantages and disadvantages of these techniques. A short outlook will also look at new techniques that could improve the ability to measure chirality changes on an ultrafast time‐scale.     

Time averaging and fitting of nonlinear metabolic changes: the issue of the time index choice applied to 31P MRS investigation of muscle energetics

We present an exact analytical method dedicated to fitting time-dependent exponential-like changes in MR spectra. As an illustration, this method has been applied to fitting metabolic changes recorded by 31P MRS in human skeletal muscle occurring during a rest-exercise-recovery protocol. When recording metabolic changes with the accumulative method, the time averaging of the MR signals implies the choice of a time index for fitting any changes in the features of the associated MR spectra. A critical examination of the different ways (constant, linear, and exponential) of choosing the time index is reported. By numerical analysis, we have calculated the errors generated by the three methods and we have compared their sensitivity to noise. In the case of skeletal muscle, both constant and linear methods introduce large and uncontrolled errors for the whole set of metabolic parameters derived from [PCr] changes. In contrast, the exponential method affords a reliable estimation of critical parameters in muscle bioenergetics in both normal and pathological situations. This method is very easy to implement and provides an exact analytical solution to fitting changes in MR spectra recorded by the accumulative method.     

Changes of summating potentials and morphology in theguinea pig cochlea during anoxia

I.IntroductionThesummatingpotcntia1s(SPs)rcprcscntsaspccificbioe1cctronphenomenonofthecoch1ea.C1inica1lythedominant-SPisnowtakenasasignofrecruitmcnt.However,themechanismofrecruitn1cnt,exccptinMcnicrcdiscase,isnotentireIyunderstood.Thedynamicchangcsinamp1itudcandpolarityofSP,lnrclationtothethrcsho1dshiftofcompoundactionpotential(CAP),SPandcndocochlcapotcntial(EP)inthescalcmcdiawcrcexaminedatonehmewithsing1cmicropipctduringandaftcranoxia.Also,themorpho1ogicchangesinthein-nerhaircc11s(IHC…