Mild hyperthermia-enhanced chemo-photothermal synergistic therapy using doxorubicin-loaded gold nanovesicles

Gold nanovesicles(GVs) with unique plasmonic property and large cavity hold great potential as a stimuli-responsive nanocarrier to deliver drugs for efficient tumor chemotherapy and other therapies synergistically.Herein,we developed doxorubicin-loaded gold nanovesicles(DGVs),offering infrared thermal(IRT) and photoacoustic(PA) dual-modal imaging guided mild hype rthermia-enhanced chemophotothermal cancer synergistic therapy.The DGVs are self-assembled by gold nanoparticles modified with amphiphilic copolymer in a predetermined concentration of doxorubicin through film rehydration method.Under the influence of laser excitation,the as-prepared DGVs exhibited good photothermal effect,which triggered the structural disruption of GVs and thus,allowed the efficient release of encapsulated DOX to enhance cell uptake for fluorescence imaging and tumor chemotherapy,respectively.In addition,DGVs also showed a strong PA and IRT signals in vivo.Our study demonstrated the potential of DGVs as stimuli-responsive drug delivery systems and cancer theranostics.     

NIR-triggered drug delivery system based on phospholipid coated ordered mesoporous carbon for synergistic chemo-photothermal therapy of cancer cells

Chemo-photothermal treatment is one of the most efficient strategies for cancer therapy. However, traditional drug carriers without near-infrared absorption capacity need to be loaded with materials behaving photothermal properties, as it results in complicated synthesis process, inefficient photothermal effects and hindered NIR-mediated drug release. Herein we report a facile synthesis of a polyethylene glycol (PEG) linked liposome (PEG-liposomes) coated doxorubicin (DOX)-loaded ordered mesoporous carbon (OMC) nanocomponents (PEG-LIP@OMC/DOX) by simply sonicating DOX and OMC in PEG-liposomes suspensions. The as-obtained PEG-LIP@OMC/DOX exhibits a nanoscale size (600 ± 15 nm), a negative surface potential (−36.70 mV), high drug loading (131.590 mg/g OMC), and excellent photothermal properties. The PEG-LIP@OMC/DOX can deliver loaded DOX to human MCF-7 breast cancer cells (MCF-7) and the cell toxicity viability shows that DOX unloaded PEG-LIP@OMC has no cytotoxicity, confirming the PEG-LIP@OMC itself has excellent biocompatibility. The NIR-triggered release studies demonstrate that this NIR-responsive drug delivery system enables on-demand drug release. Furthermore, cell viability results using human MCF-7 cells demonstrated that the combination of NIR-based hyperthermal therapy and triggered chemotherapy can provide higher therapeutic efficacy than respective monotherapies. With these excellent features, we believe that this phospholipid coating based multifunctional delivery system strategy should promote the application of OMC in nanomedical applications.     

NIR-triggered drug delivery system based on phospholipid coated ordered mesoporous carbon for synergistic chemo-photothermal therapy of cancer cells

Chemo-photothermal treatment is one of the most efficient strategies for cancer therapy. However, traditional drug carriers without near-infrared absorption capacity need to be loaded with materials behaving photothermal properties, as it results in complicated synthesis process, inefficient photothermal effects and hindered NIR-mediated drug release. Herein we report a facile synthesis of a polyethylene glycol (PEG) linked liposome (PEG-liposomes) coated doxorubicin (DOX)-loaded ordered mesoporous carbon (OMC) nanocomponents (PEG-LIP@OMC/DOX) by simply sonicating DOX and OMC in PEG-liposomes suspensions. The as-obtained PEG-LIP@OMC/DOX exhibits a nanoscale size (600±15 nm), a negative surface potential (-36.70 mV), high drug loading (131.590 mg/g OMC), and excellent photothermal properties. The PEG-LIP@OMC/DOX can deliver loaded DOX to human MCF-7 breast cancer cells (MCF-7) and the cell toxicity viability shows that DOX unloaded PEG-LIP@OMC has no cytotoxicity, confirming the PEG-LIP@OMC itself has excellent biocompatibility. The NIR-triggered release studies demonstrate that this NIR-responsive drug delivery system enables on-demand drug release. Furthermore, cell viability results using human MCF-7 cells demonstrated that the combination of NIR-based hyperthermal therapy and triggered chemotherapy can provide higher therapeutic efficacy than respective monotherapies. With these excellent features, we believe that this phospholipid coating based multifunctional delivery system strategy should promote the application of OMC in nanomedical applications.     

Eu^2＋和Mn^2＋在Sr3MgSi2O8中的光致发光研究

研究了Ｅｕ＾２＋和Ｍｎ＾２＋共激活的Ｓｒ３ＭｇＳｉ２Ｏ８的荧光性质。Ｅｕ＾２＋和Ｍｎ＾２＋在４６０ｎｍ和６９０ｎｍ的发射峰分别由Ｅｕ＾２＋的５ｄ→４ｆ跃迁和Ｍｎ＾２＋的＾４Ｔ１（＾４Ｇ）→＾６Ａ１ｇ（＾６Ｓ）跃迁产生。未观察到单掺杂Ｍｎ＾２＋的Ｓｒ３ＭｇＳｉ２Ｏ８的荧光发射，而掺入Ｅｕ＾２＋后则出现了Ｍｎ＾２＋的６９０ｎｍ光致发光峰，表明Ｅｕ＾２＋对Ｍｎ＾２＋有敏化作用。Ｅｕ＾２＋的荧光寿命也受Ｍ     

化学发光消耗型锰传感器

化学和生物发光是由化学反应产生的一种光辐射,不需要任何光源。又由于它们具有高灵敏度、宽线性范围和相对比较便宜的仪器等优点,因而在化学和生物传感器领域引起了广泛的兴趣。已用于H_2O_2、乳酸和胆固醇等多种生物活性物质的测定,但未见有金属离子传感器的报道。本文发展了一种新型的全固态模式的消耗型锰离子化学发光传感器。该传感器将除待测物外的所有化学发光反应试剂全部固定在阴离子交换树脂Amberlyst A-27上,于化学发光反应之前,将一定量化学发光试剂从固定化试剂柱上洗脱,与样品中的锰离子产生化学发光。已成功地应用于水样中痕量锰离子的测定。每个固定化试剂柱可连续使用100次以上。 1 实验部分 1.1 仪器和试剂 化学发光传感器由流动系统和检测系统两部分组成。其中流动系统主要由蠕动泵、六通阀、固定化试剂柱和流通池组成。检测系统由光电信增管、负高压、放大器和记录仪组成(图1)。     

Eu~(2+),Mn~(2+)共激活碱土镁硅酸盐基红色荧光粉的发光性能

制备了以R3MgSi2O8(R=Ba,Sr,Ca)为基,Eu2+,Mn2+共激活的红色荧光粉并研究了其荧光性质。分别以Ba3MgSi2O8,Sr3MgSi2O8,Ca3MgSi2O8为基质时,由于晶体场环境不同,发光强度、发射峰产生相应变化。研究了以(Ba,Sr)3MgSi2O8为基的荧光粉中Ba,Sr相对量,及Eu2+,Mn2+浓度对发光性质的影响并探讨了Eu2+,Mn2+在基质中所处格位;结果表明,红光是由基质中处于九配位的Eu2+将能量传递给八面体六配位的Mn2+,而由Mn2+所发射的。     

Polydopamine(PDA)-activated cobalt sulfide nanospheres responsive to tumor microenvironment(TME)for chemotherapeutic-enhanced photothermal therapy

Most recently,cobalt sulfide(CoS) nanospheres(NSs) have been demonstrated as an ideal high-efficient photothermal agent for tumor elimination.Howeve r,the surface of CoS NSs is lack of functio nal chemical groups or active radicals to incorporate therapeutic agents,which tremendously hinders their versatile utilization in medical field.Here,surface activation of CoS NSs was realized through the growth of polydopamine(PDA) in situ via alkaline-triggered polymerization.Upon the formation of CoS@PDA NSs,thiol-polyethylene glycol(SH-PEG) and chemotherapeutic agent of doxorubicin(DOX) were loaded onto the particle surface by means of π-π electrostatic interaction and Michael addition reactions.Assynthesized CoS@PDA/PEG/DOX(CoPPD) NSs exhibited an admirable photothermal property and high loading capacity of DOX(44.6%).Furthermore,drug release can be accelerated under a more acidic pH condition mimicking tumor microenvironment(TME),ascribed to the protonation of amino group in DOX molecules.Finally,a strong chemotherapeutic-enhanced photothermal therapeutic effect was demonstrated toward solid tumor under near-infrared(NIR) light irradiation without causing significant systemic toxicity.In this regard,this paradigm may offer valuable guidance for the design of multifunctional CoS-based nanoagents for medical treatment.     

锰离子掺杂对LiCoPO4性能的影响

应用溶胶-凝胶法合成了锰掺杂的LiCoPO4正极材料.X射线衍射、扫描电镜和循环伏安等电化学测试表明,少量锰离子掺杂不影响LiCoPO4的晶格结构,且明显改善了LiCoPO4正极材料电化学性能.锰掺杂量为1%时得到的LiMn0.01Co0.99PO4正极材料具有最好的电化学性能,以0.1C倍率放电时,首次放电比容量可达130.6 mAh/g.     

Polyethylene glycol phospholipids encapsulated silicon 2,3-naphthalocyanine dihydroxide nanoparticles (SiNcOH-DSPEPEG(NH2) NPs) for single NIR laser induced cancer combination therapy

Currently, the combination of photothermal therapy (PTT) and photodynamic therapy (PDT) has emerged as a powerful technique for cancer treatment. However, most examples of combined PTT and PDT reported use multi-component nanocomposites under excitation of separate wavelength, resulting in complex treatment process. In this work, a novel theranostic nanoplatform (SiNcOH-DSPE-PEG(NH₂) NPs) has been successfully developed by coating silicon 2,3-naphthalocyanine dihydroxide (SiNcOH) with DSPE-PEG and DSPE-PEG-NH₂ for photoacoustic (PA) imaging-guided PTT and PDT tumor ablation for the first time. The as-prepared single-agent SiNcOH-DSPE-PEG(NH₂) NPs not only have good water solubility and biocompatibility, but also exhibit high photothermal conversion efficiency and singlet oxygen generation capability upon 808 nm NIR laser irradiation. In addition, owing to their high absorption at NIR region, the SiNcOH-DSPE-PEG(NH₂) NPs can also be employed as an effective diagnostic nanoagent for photoacoustic (PA) imaging. In vitro and in vivo experimental results clearly indicated that the simultaneously combined PTT and PDT under the guidance of PA imaging with single NIR laser excitation can effectively kill cancer cells or eradicate tumor tissues. Taking facile synthesis and high efficiency in cancer treatment by SiNcOH-DSPE-PEG(NH₂) NPs into consideration, our study provides a promising strategy to realize molecular imaging-guided combination therapy.     

Magnetic and structural investigation of Mn(2+)-doped ZnSe semiconductor nanoparticles.

X-ray magnetic circular dichroism (XMCD) experiments on diluted magnetic semiconductor nanocrystals were carried out to study the local electronic structure and magnetic properties of Mn(2+) embedded in the lattice of ZnSe nanoparticles. It is shown that Mn(2+) is exclusively present in the bulk of ZnSe nanoparticles. Neither Mn-Mn coupling nor traces of oxidation to higher Mn oxidation states was observed. This result, which is consistent with EPR spectroscopic data, provides clear proof of the location of Mn(2+) in semiconductor nanoparticles. Further, it is shown that the magnetic ions are highly polarised inside the nanocrystals, where they reach about 50 % of the theoretical value of a pure d(5) state under identical conditions.     

碳纳米管与CTMAB协同加强荧光探针并应用于测定痕量锰

提出将碳纳米管(CNT)作为一种新型分析增效试剂用于加强荧光探针,研究了其与阳离子表面活性剂-十六烷基溴化铵(CTMAB)的协同增敏机理,并应用于苯基荧光酮(PF)荧光猝灭法测定锰离子的体系中。结果表明,在CNT与CTMAB复配的影响下,PF与Mn2+形成的络合物荧光猝灭强度最大,据此建立了CNT加强荧光猝灭法测定痕量锰的新方法。方法的检出限为0.2μg/L,线性范围为0.4~10μg/L,回归方程:ΔF=10.48ρ(μg/L)+10.77,R=0.9992。本法可用于自来水中Mn2+含量的检测。     

2-(2-羟基-5-氯苯基)-1H-苯并咪唑-铜(Ⅱ)配合物作为NO荧光探针的初探

常温下合成了2-(2-羟基-5-氯苯基)-1H-苯并咪唑荧光化合物和其二价铜离子的配合物,并对配合物的结构和稳定性进行了表征,用荧光光谱、质谱、红外光谱等研究了配合物与NO反应的机理.结果表明,该配合物与NO的反应具有高度的选择性,不受其他常见干扰分子的影响.配合物应用于脂多糖(LPS)激活的小鼠巨噬细胞中NO的测定,能够得到具有较好分辨率的荧光成像结果.     

PEGylated BODIPY assembling fluorescent nanoparticles for photodynamic therapy

Two PEGylated BODIPY which could self-assemble into nanoparticles were synthesized via multicomponent Passerini reaction for cellular imaging and photodynamic therapy.     

半青蓝素修饰汞离子探针的比色传感行为

合成了以半青蓝素为发光基团,分别以Se2N和S2N为结合位点的比色传感器3a和3b,通过紫外-可见光谱研究了它们对各种金属离子的化学传感行为. 结果表明,与含有S原子的主体分子3b相比,含有Se杂原子的主体3a对Hg2+具有较好的识别作用.向主体3a的乙腈水溶液中加入Hg2+后,其吸收光谱在410 nm处出现了一个新的吸收峰,同时溶液颜色由玫瑰红色变为桔黄色. 因此,化合物3a有望成为一种用于检测Hg2+的新型化学传感器.     

pH/光热双重响应的纳米递送系统构建及对耐药膀胱癌细胞的联合治疗

以介孔聚多巴胺(MPDA)的制备为出发点,通过搭载化疗药物阿霉素(DOX)和包覆相变材料 1-十四醇(PCM),构建了 pH/光热双重响应的 MPDA-DOX@PCM 纳米递送系统,实现了对耐药膀胱癌细胞(BIU-87/ADR)的光热治疗(PTT)和化疗。结果表明,MPDA-DOX@PCM 尺寸约为 179 nm,DOX的最大搭载率为 22%,光热转换效率高达 49.1%。在 pH=7.4和温度为 25 ℃的条件下,DOX的累积释放率为4.57%;当pH值降为5.5和温度升高到45 ℃时,DOX的累积释放率可提高到60.13%。在808 nm激光辐照下,MPDA-DOX@PCM孵育的BIU-87/ADR细胞存活率降低至9.5%,证明了其优异的PTT/化疗联合治疗性能。     

Protein nanoparticles containing Cu(II) and DOX for efficient chemodynamic therapy via self-generation of H2O2

Chemodynamic therapy (CDT) refers to generating hydroxyl radical (OH) in tumor sites via hydrogen peroxide (H₂O₂) catalyzed by transition metal ions in cancer cells under acidic environment. However, H₂O₂ content is not enough for effective CDT, although H₂O₂ content in cancer cells is higher than that of normal cells. Herein, we synthesized DOX@BSA-Cu NPs (nanoparticles) for effective CDT by providing enhanced content of H₂O₂ in cancer cells. The results proved Cu²⁺ in NPs could be reduced to Cu⁺ by glutathione (GSH) and effectively converted H₂O₂ to OH. Moreover, the loaded low-dose doxorubicin (DOX) in the NPs could improve the content of H₂O₂ and resulted in more efficient generation of OH in cancer cells. Thus DOX@BSA-Cu NPs exhibited higher cytotoxicity to cancer cells. This research may provide new ideas for the further studies on more effective Cu(II)-based CDT nanoagents.