Spectral modulation of charge transfer fluorescence probe encapsulated inside aqueous and non-aqueous β-cyclodextrin nanocavities

In this work, we report the spectral modulations of a intramolecular charge transfer (ICT) molecule ethyl ester of N,N-dimethylaminonaphthyl-(acrylic)-acid (EDMANA) when encapsulated in the water and N,N-dimethylformamide (DMF) solution of β-CD nanocavities. From the nature of the Benesi-Hildebrand (B-H) plots, the stoichiometry of the host guest inclusion complexes are found to be 1:1 in water β-CD solution and both 1:1 and 1:2 in DMF β-CD solution. The preferential location and difference in orientation of EDMANA molecule inside the β-CD cavity has been accessed by analysis of the effect of acid and metal cation Ni²⁺ on the spectral characteristics in both the media. In case of 1:1 complex, the polar donor group prefers to expose to bulk aqueous phase capable of binding with H⁺ and Ni²⁺ ions and the acceptor to the hydrophobic interior. On the other hand, the acceptor group remains exposed to the non-polar bulk phase and the donor group is orientated preferentially inside the non-polar core in 1:2 inclusion complexes.     

MUC1 vaccines using β-cyclodextrin grafted chitosan (CS-g-CD) as carrier via host-guest interaction elicit robust immune responses

We construct MUC1 vaccines using β-cyclodextrin grafted chitosan (CS-g-CD) as carrier via host-guest interaction. These vaccines based on non-covalent assembling can provoke robust immune responses, including high level of specific antibodies and cytokines. The induced antibodies can specifically recognize tumor cells and mediate cytotoxicity against tumor cells. These results indicate that CS-g-CD with strong immunostimulatory activities can be a straightforward platform for peptide-based vaccine construction.     

Synthetic β-(1→3)-<Emphasis Type="SmallCaps">d</Emphasis>-glucooligosaccharides: model compounds for the mechanistic study of β-(1→3)-<Emphasis Type="SmallCaps">d</Emphasis>-glucan bioactivities and design of antifungal vaccines

Synthetic methods used for the preparation of linear β-(1→3)-d-glucooligosaccharides with three and more monosaccharide units and their conjugates with carrier proteins, as well as the application of such derivatives in the mechanistic study of bioactivites of natural β-(1→3)-d-glucans and in the design of conjugated antifungal vaccines are considered.     

Synthesis,characterisation and antibacterial studies of carbon nanoparticle and carbon nanoparticle encapsulated in functionally modified β-cyclodextrin with {5-[4-(dimethylamino) benzylidene]-4-oxo-2-thioxo-1, 3-thiazolidin-3-yl} acetic acid

This paper describes the synthesis of carbon nanoparticle (CNP) from natural sources such as kitchen soot, synthesis of a chromophoric system {5-[4-(dimethylamino) benzylidene]-4-oxo-2-thioxo1, 3-thiazolidin-3-yl} acetic acid and its incorporation into β-cyclodextrin (β-CD) through the esterification of the hydroxyl group with the free carboxyl function of the chromophoric system by DCC coupling. Encapsulation of CNP into functionally modified β-CD system was achieved. Characterisation of the products was undertaken by UV–visible, FT-IR, NMR, fluorescence spectroscopic methods, SEM, TEM and X-ray diffraction methods. Their antibacterial activities were addressed. The antibacterial activity of CNP and functionally modified β-CD-CNP products were tested against various pathogenic bacterial strains such as Streptcoccus haemoliticus (+ve), Staphylococcus aureus (+ve), Klebsiella pneumonia (?ve) and E. coli (MTCC1687) (?ve) by disc diffusion method. The results showed that the CNPs and functionally modified products have good antibacterial activity against selected pathogenic bacterial strains.