Co-precipitation of asiatic acid and poly(<Emphasis Type="SmallCaps">l</Emphasis>-lactide) using rapid expansion of subcritical solutions into liquid solvents

Poly(l-lactide) (PLLA) nanoparticles loaded with asiatic acid (AA) were successfully produced by rapid expansion of a subcritical solution into an aqueous receiving solution containing a dispersing agent. A mixture of carbon dioxide (CO₂) and ethanol (EtOH) with a weight ratio of 1:1 was used as the solvent for AA and PLLA. Two surfactants, Pluronic F127 and sodium dodecyl sulfate were employed. The former was found to be more effective for stabilizing AA-loaded PLLA nanoparticles, as a rapid expansion into a 0.1 wt% Pluronic F127 solution produced a stable nanosuspension consisting mainly of well-dispersed, individual nanoparticles. The effects of rapid expansion-processing conditions—AA to PLLA weight ratio and pre-expansion temperature (T_pre)—on the size and morphology of composite nanoparticles, and the loading capacity and entrapment efficiency of AA in PLLA nanoparticles, were systematically investigated. It was found that AA-loaded PLLA nanoparticles with a size range of 30–100 nm were consistently fabricated by rapid expansion at T_pre of 70–100 °C and AA to PLLA weight ratios of 1:2 and 1:4, and with a constant pre-expansion pressure of 330 bar. The T_pre and AA to PLLA weight ratio had no significant effects on the size of the nanoparticles. The AA to PLLA weight ratio is a controlling parameter for both the loading capacity and the entrapment efficiency of AA in PLLA nanoparticles. The loading capacity and entrapment efficiency increased from 8–11 to 16–21 wt%, and 38–57 to 50–62 wt%, respectively, when the AA to PLLA weight ratio changed from 1:4 to 1:2. However, increasing the T_pre from 70 to 100 °C decreased both the loading capacity and entrapment efficiency of AA in PLLA nanoparticles by ~20–30%.     

Evaluation of the antibacterial activity of poly-(<Emphasis Type="SmallCaps">d</Emphasis>,<Emphasis Type="SmallCaps">l</Emphasis>-lactide-co-glycolide) nanoparticles containing violacein

Since violacein—an antibiotic, antiviral, and antiparasitic compound—exhibits poor solubility in water, polymeric poly-(d,l-lactide-co-glycolide) nanoparticles containing this compound improved its solubility and biological activity. The nanoparticles were prepared by the nanoprecipitation method and characterized in terms of average diameter, zeta potential, drug loading, polymer recovery, in vitro release kinetic, and in vitro antibacterial activity. Nanoparticles with diameters between 116 and 139 nm and negative-charged outer surfaces were obtained. Drug-loading efficiency and polymer recovery were 87 and 93%, respectively. In vitro release kinetics assays showed that violacein loaded in these nanoparticles has sustained release behavior until 5 days. Both free and nanoparticles-loaded violacein exhibited in vitro antibacterial activity against Staphylococcus aureus ATCC 29213 and ATCC 25923 strains and exhibiting around two to five times lower minimum inhibitory concentration (MIC) than free violacein, respectively. The encapsulated violacein was efficient against methicilin-resistant Staphylococcus aureus (MRSA) strains. No significant activity against Escherichia coli and Salmonella enterica was found.     

Maghemite/poly(<Emphasis Type="SmallCaps">d,l</Emphasis>-lactide-<Emphasis Type="Italic">co</Emphasis>-glycolyde) composite nanoplatform for therapeutic applications

A reproducible methodology is described for the synthesis, by following the double emulsion/solvent evaporation technique, of magnetic nanocomposites (average diameter ≈ 135 nm) consisting of maghemite nuclei and a biodegradable poly(d,l-lactide-co-glycolide) matrix. The heterogeneous structure of the nanoparticles can confer them the responsiveness to magnetic gradients, giving both the possibility of their use as a drug delivery system and adequate heating characteristics for a hyperthermia effect. The physical chemistry of the nanocomposites was extensively characterized, this establishing that their surface properties were similar to that of pure poly(d,l-lactide-co-glycolide). From an electrokinetic point of view, zeta potential determinations (as a function of the ionic strength, and pH) pointed out that the nanocomposites were almost indistinguishable from the copolymer. The surface thermodynamic analysis agreed with the electrophoretic one in suggesting that the coverage of the magnetic nuclei was complete, since the hydrophilic nature of maghemite was modified and the nanoparticles turned into hydrophobic, just like the copolymer, when they were embedded into poly(d,l-lactide-co-glycolide). The magnetic behaviours of the composite nanoparticles were also checked. Their heating properties were studied in vitro in a high-frequency alternating gradient of magnetic field: a stable maximum temperature of 47 °C was satisfactorily achieved within 45 min. Blood compatibility of the nanocomposites was also defined in vitro. To our knowledge, this is the first time that such kind of magnetic-sensitive nanoformulation with very promising characteristics (e.g. blood compatibility, magnetic drug targeting capabilities, and hyperthermia) has been developed for therapeutic purposes.     

Polynomial Realization of <Emphasis Type="Italic">s</Emphasis>l<Subscript><Emphasis Type="Italic">q</Emphasis></Subscript>(2) and Fusion Rules at Exceptional Values of <Emphasis Type="Italic">q</Emphasis>

Representations of the sℓ_q(2) algebra are constructed in the space of polynomials of real (complex) variable for q^N=1. The spin addition rule based on eigenvalues of Casimir operator is illustrated on few simplest cases and conjecture for general case is formulated.     

<Emphasis Type="Italic">f</Emphasis>(<Emphasis Type="Italic">R</Emphasis>) Cosmology from <Emphasis Type="Italic">q</Emphasis>-theory

From a macroscopic theory of the quantum vacuum in terms of conserved relativistic charges (generically denoted by q ^(a) with label a), we have obtained, in the low-energy limit, a particular type of f(R) model relevant to cosmology. The macroscopic quantum-vacuum theory allows us to distinguish between different phenomenological f(R) models on physical grounds. The text was submitted by the authors in English.     

Shell effects for isovector <Emphasis Type="Italic">l</Emphasis>-forbidden <Emphasis Type="Italic">M</Emphasis>1 transitions in odd nuclei

It is inferred from the analysis of the experimental data that the reduced probabilities of isovector l-forbidden M1 transitions achieve maximum values in nuclei with a magic core. The minimum values are related to γ transitions in nuclei belonging to the region of stable deformation with A ≈ 25. Isovector l-forbidden M1 transitions in mirror nuclei are explored. The ratio of the reduced probabilities for proton and neutron transitions shows the maximum deviation from the theoretical evaluations for the pair of nuclei ⁴¹Ca-⁴¹Sc consisting of the even-even magic core ⁴⁰Ca and one nucleon outside the closed 1d2s shell.     

Preparation of nano-hydroxyapatite/poly(<Emphasis Type="SmallCaps">l</Emphasis>-lactide) biocomposite microspheres

Nano-hydroxyapatite (HA)/poly(l-lactide) (PLLA) composite microspheres with relatively uniform size distribution were prepared by a solid-in-oil-in-water (s/o/w) emusion solvent evaporation method. The encapsulation of the HA nanopaticles in microshperes was significantly improved by grafting PLLA on the surface of the HA nanoparticles (p-HA) during emulsion process. This procedure gave a possibility to obtain p-HA/PLLA composite microspheres with uniform morphology and the encapsulated p-HA nanoparticle loading reached up to 40 wt% (33 wt% of pure HA) in the p-HA/PLLA composite microspheres. The microstructure of composite microspheres from core-shell to single phase changed with the variation of p-HA to PLLA ratios. p-HA/PLLA composite microspheres with the diameter range of 2–3 μm were obtained. The entrapment efficiency of p-HA in microspheres could high up to 90 wt% and that of HA was only 13 wt%. Surface and bulk characterizations of the composite microspheres were performed by measurements such as wide angle X-ray diffraction (WAXD), thermal gravimetric analysis (TGA), environmental scanning electron microscope (ESEM) and transmission electron microscopy (TEM).     

Electrocatalytic oxidation of <Emphasis Type="SmallCaps">l</Emphasis>-tyrosine at carboxylic acid functionalized multi-walled carbon nanotubes modified carbon paste electrode

The electrocatalytic oxidation of l-tyrosine (Tyr) was investigated on a carboxylic acid functionalised multi-walled carbon nanotubes modified carbon paste electrode using cyclic voltammetry and amperometry. The surface morphology of the electrodes was studied using field emission (FE)-SEM images, and the interface properties of bare and modified electrodes were investigated by electrochemical impedance spectroscopy (EIS). The influence of the amount of modifier loading and the variation of the pH of the solution on the electrochemical parameters have been investigated. Cyclic voltammetry was carried out to study the electrochemical oxidation mechanism of Tyr, which showed an irreversible oxidation process at a potential of 637.0 mV at modified electrode. The anodic peak current linearly increased with the scan rate, suggesting that the oxidation of Tyr at modified electrode is an adsorption-controlled process. A good linear relationship between the oxidation peak current and the Tyr concentration in the range of 0.8–100.0 μM was obtained in a phosphate buffer solution at pH 7.0 with a detection limit of 14.0?±?1.36 nM (S/N?=?3). The practical utility of the sensor was demonstrated by determining Tyr in spiked cow’s milk and human blood serum. The modified electrode showed excellent reproducibility, long-term stability and antifouling effects.     

Non-vacuum Bianchi types <Emphasis Type="Italic">I</Emphasis> and <Emphasis Type="Italic">V</Emphasis> in <Emphasis Type="Italic">f</Emphasis> (<Emphasis Type="Italic">R</Emphasis>) gravity

In a recent paper (Sharif and Shamir in Class. Quantum Grav. 26:235020, 2009), we have studied the vacuum solutions of Bianchi types I and V spacetimes in the framework of metric f (R) gravity. Here we extend this work to perfect fluid solutions. For this purpose, we take stiff matter to find energy density and pressure of the universe. In particular, we find two exact solutions in each case which correspond to two models of the universe. The first solution gives a singular model while the second solution provides a non-singular model. The physical behavior of these models has been discussed using some physical quantities. Also, the function of the Ricci scalar is evaluated.     

Multifunctional antitumor magnetite/chitosan-<Emphasis Type="SmallCaps">l</Emphasis>-glutamic acid (core/shell) nanocomposites

The development of anticancer drug delivery systems based on biodegradable nanoparticles has been intended to maximize the localization of chemotherapy agents within tumor interstitium, along with negligible drug distribution into healthy tissues. Interestingly, passive and active drug targeting strategies to cancer have led to improved nanomedicines with great tumor specificity and efficient chemotherapy effect. One of the most promising areas in the formulation of such nanoplatforms is the engineering of magnetically responsive nanoparticles. In this way, we have followed a chemical modification method for the synthesis of magnetite/chitosan-l-glutamic acid (core/shell) nanostructures. These magnetic nanocomposites (average size ≈340 nm) exhibited multifunctional properties based on its capability to load the antitumor drug doxorubicin (along with an adequate sustained release) and its potential for hyperthermia applications. Compared to drug surface adsorption, doxorubicin entrapment into the nanocomposites matrix yielded a higher drug loading and a slower drug release profile. Heating characteristics of the magnetic nanocomposites were investigated in a high-frequency alternating magnetic gradient: a stable maximum temperature of 46 °C was successfully achieved within 40 min. To our knowledge, this is the first time that such kind of stimuli-sensitive nanoformulation with very important properties (i.e., magnetic targeting capabilities, hyperthermia, high drug loading, and little burst drug release) has been formulated for combined antitumor therapy against cancer.     

<Emphasis Type="Italic">V</Emphasis>-<Emphasis Type="Italic">A</Emphasis> sum rules with <Emphasis Type="Italic">D</Emphasis> = 10 operators

The difference of vector and axial-vector charged current correlators is analyzed by means of QCD sum rules. The contribution of 10-dimensional 4-quark condensates is calculated and its value is estimated within the framework of the factorization hypothesis. It is compared to the result obtained from an operator fit of Borel sum rules in the complex q ²-plane, calculated from experimental data on hadronic -decays. This fit gives accurate values of the light quark condensate and the quark-gluon mixed condensate. The size of the high-order operators and the convergence of the operator series are discussed.Received: 10 May 2004, Revised: 7 September 2004, Published online: 18 November 2004     

Ferromagnetism and ferroelectricity in Eu<Emphasis Type="Italic">X</Emphasis> (<Emphasis Type="Italic">X</Emphasis> = <Emphasis Type="Italic">O</Emphasis>, <Emphasis Type="Italic">S</Emphasis>): pressure effects

Ferromagnetism and ferroelectricity in Eu monochalcogenides have been investigated by ab initio density functional theory in the DFT+U approach. Exchange interaction parameters and Curie temperatures under pressure are studied and discussed using Heisenberg Hamiltonian with first and second-nearest-neighbor interactions. The calculations showed that the hydrostatic pressure perfectly improves the Curie temperature (EuO: T _C = 175 K; EuS: T _C = 33.8 K) and in the other hand it cannot induce the spontaneous polarization (P _s ). The effect of uniaxial and biaxial pressure is also studied. Although the uniaxial strains slightly increases the Curie temperature, it ensures the ferrolectricity in these systems by producing a spontaneous polarization of the order of P _s (EuO) = 57.50 μC/cm² and P _s (EuS) = 42.86 μC/cm² with pressures of 5% and 4%, respectively. The search for new model systems is a necessity to better understand the physics related to multiferroïc materials and to consider possible applications.     

Spherical Symmetric Perfect Fluid Collapse in <Emphasis Type="Italic">f</Emphasis>(<Emphasis Type="Italic">R</Emphasis>, <Emphasis Type="Italic">T</Emphasis>) Gravity

This paper contains the study of spherically symmetric perfect fluid collapse in the frame work of f(R, T) modified theory of gravity. We proceed our work by considering the non-static spherically symmetric background in the interior and static spherically symmetric background in the exterior regions of the star. The junction conditions between exterior and interior regions are presented by matching the exterior and interior regions. The field equations are solved by taking the assumptions that the Ricci scalar as well as the trace of energy-momentum tensor are to be constant, for a particular f(R, T) model. By inserting the solution of the field equations in junction conditions, we evaluate the gravitational mass of the collapsing system. Also, we discuss the apparent horizons and their time formation for different possible cases. It is concluded that the term f(R ₀, T ₀) behaves as a source of repulsive force and that’s why it slowdowns the collapse of the matter.     

Energy dependence of Cronin momentum in saturation model for <Emphasis Type="Italic">p</Emphasis> + <Emphasis Type="Italic">A</Emphasis> and <Emphasis Type="Italic">A</Emphasis> + <Emphasis Type="Italic">A</Emphasis> collisions

The energy dependence of the Cronin momentum for p + A and A + A collisions in the saturation model are calculated. This dependence is consistent with simple dimensional considerations and can be used to test the validity of the saturation model. It gives the possibility to distinguish the different variants of the saturation model with precise experimental data and to measure the x dependence of the saturation momentum.     

<Emphasis Type="Italic">f</Emphasis> (<Emphasis Type="Italic">R</Emphasis>) black holes

We study the f (R)-Maxwell black hole imposed by constant curvature and its all thermodynamic quantities, which may lead to the Reissner-Nordström-AdS black hole by redefining Newtonian constant and charge. Further, we obtain the f (R)-Yang-Mills black hole imposed by constant curvature, which is related to the Einstein-Yang-Mills black hole in AdS space. Since there is no analytic black hole solution in the presence of Yang-Mills field, we obtain asymptotic solutions. Then, we confirm the presence of these solutions in a numerical way.     

Reconstruction of some cosmological models in <Emphasis Type="Italic">f</Emphasis>(<Emphasis Type="Italic">R</Emphasis>,<Emphasis Type="Italic">T</Emphasis>) cosmology

In this paper, we reconstruct cosmological models in the framework of f(R,T) gravity, where R is the Ricci scalar and T is the trace of the stress-energy tensor. We show that the dust fluid reproduces ΛCDM, phantom–non-phantom era and phantom cosmology. Further, we reconstruct different cosmological models, including the Chaplygin gas, and scalar field with some specific forms of f(R,T). Our numerical simulation for the Hubble parameter shows good agreement with the BAO observational data for low redshifts, z<2.     

<Emphasis Type="Italic">EPJdirect</Emphasis>

Quark mass effects are analyzed at high Q² in the current fragmentation region of DIS. It is found that the linear combination F ₂ -2.75F ^c ₂ scales at large Q² and small x. We obtained a lower bound for the ratio F ^c ₂/F ₂ which lies very close to the data from HERA. Received: 14 January 2002 / Revised version: 9 October 2002 Published online: 9 December 2002 RID="a" ID="a" e-mail: ryutin@th1.ihep.su     

<Emphasis Type="Italic">O</Emphasis>(1) eV sterile neutrino in <Emphasis Type="Italic">f</Emphasis>(<Emphasis Type="Italic">R</Emphasis>) gravity

We refer [1] to the role of an additional O(1) eV sterile neutrino in modified gravity models. We find parameter constraints in particular f(R) gravity model using following up-to-dated cosmological data: measurements of the cosmic microwave background (CMB) anisotropy, the CMB lensing potential, the baryon acoustic oscillations (BAO), the cluster mass function and the Hubble constant. It was obtained for the sterile neutrino mass 0.47 eV < m _ν,sterile < 1 eV (2σ) assuming that the sterile neutrinos are thermalized and the active neutrinos are massless, not significantly larger than in the standard cosmology model within the same data set: 0.45 eV < m _ν,sterile < 0.92 eV (2σ). But, if the mass of sterile neutrino is fixed and equals ≈ 1.5 eV according to various anomalies in neutrino oscillation experiments, f(R) gravity is much more consistent with observation data than the CDM model.