A simple one‐step electrodeposition method is described to fabricate three dimensional ordered macroporous chitosan?prussian blue?single walled carbon nanotubes (3DOM CS?PB?SWCNTs) film onto the gold electrode surface to fabricate a copper ions (Cu2+)‐specific DNAzyme biosensor. The new sensing strategy for sensitive and selective detection of Cu2+ was based on Au nanorods (AuNRs) as signal amplification labels. The electrochemical signal of glucose increased with the concentration of Cu2+ increasing. The morphologies and electrochemistry of the composites were investigated by using scanning electron microscopy (SEM), transmission electron microscopy (TEM) and electrochemical techniques including cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) and so on. Linear correlations of copper ion concentration were obtained in the range from 10?18 M to 10?5 M, achieving with a limit of detection of 10?19 M (S/N=3). Parameters affecting the biosensor response such as temperature, the cleavage time and the time of hybridization were optimized. This biosensor showed a wide range, low detection limit, good reproducibility and high stability. Additionally, these striking properties endow the biosensor with a great promise for analytical applications. 相似文献
The present study investigates the synthesis and effectiveness of gold/gelatin nanoparticles (NPs) biopolymer as a carrier for methotrexate (MTX) drug. Two different shapes of gold particles, including spherical AuNPs (50 & 100 nm) and gold nanorods (AuNRs) with three different sizes (20, 50 and 100 nm length) were synthesized using the chemical reduction method. The effect of AuNPs size and shape on the entrapment efficiency (E.E), the release rate of the drug, and cellular uptake were investigated. The surfaces of both AuNPs and AuNRs were coated with a gelatin biopolymer, and the stability and property of the generated compounds were studied. Moreover, MTX as a chemotherapeutic agent was loaded on the gelatin-coated AuNPs/AuNRs complexes. The physicochemical properties of the gelatin-coated AuNPs/AuNRs complexes were studied using ultraviolet-visible (UV–Vis) spectroscopy, dynamic light scattering (DLS), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and Fourier transform infrared (FT-IR) spectroscopy. The E.E and MTX release behavior from the complexes at pH values of 7.4 and 5.4 and temperatures of 37 and 40 °C were investigated in vitro. The cytotoxic effects of AuNPs, AuNPs-Gelatin, AuNPs-Gelatin-MTX, AuNRs, AuNRs-Gelatin, AuNRs-Gelatin-MTX and free MTX were studied. The results indicated that the E.E of AuNPs was higher than that of AuNRs. The highest release rate of the drug was related to the AuNR1-gelatin complex (pH 5.4 and temperature of 40 °C). In addition, MTX loaded AuNR2-gelatin showed the highest cytotoxic effect on the MCF-7 breast cancer cell line so that even its cell cytotoxicity was more than that of the free drug. 相似文献
Gold nanorods (AuNRs) were obtained via a wet chemistry technique, in aqueous medium, employing crystallisation seeds. The kinetics of formation, the aspect ratio, and the selectivity of the particles were evaluated according to the parameters of synthesis: the growth-driving agent, seed, and gold precursor concentrations. In 2–4 h, the rod particles attained the expected size and shape under kinetic control, and were stable for at least 2 days. In order to obtain good quality AuNRs in good yields, without enrichment, we suggest keeping the growth-driving agent/gold molar ratio, the AuI/seed ratio, and the concentration of the reagents in the final solution within specific ranges. For example, even if good molar ratios between the reagents are maintained, relatively highly concentrated reaction solutions lead to AuNRs with lower aspect ratios. The main properties of the prepared colloidal systems and the nanoparticles were evaluated by UV–vis spectroscopy and transmission electron microscopy, respectively. 相似文献
In this study, branched polyethyleneimine (PEI) was covalently linked to carboxylic acid functionalized graphene (GO‐COOH) to form GO‐COOH/PEI composites. Transmission electron microscopy, Fourier transform infrared spectroscopy, Raman spectra and thermogravimetric analysis were used to characterize the obtained composites. Electrochemical measurements indicated that the modification of the composites on the electrode could efficiently enhance the voltammetric response, suggesting the potential application for making electrochemical sensors. Moreover, our results also indicated that the electrocatalytic oxidation of ammonia could be observed on the GO‐COOH/PEI composites modified glassy carbon electrode. Consequently, our observations demonstrated that GO‐COOH/PEI composites were excellent materials for electrochemical sensing. 相似文献
We have observed the rotational dynamics of single protein‐coated gold nanorods (AuNRs) on C18‐modified silica surfaces in real time by dual‐channel polarization dark‐field microscopy. Four different rotational states were identified, depending on the apparent strength of interactions between the AuNRs and the surface. The distributions of the states could be regulated by adjusting the salt concentration, and the state transitions were verified by monitoring the entire desorption process of a single AuNR. Our study provides insight into the interfacial orientation and dynamics of nanoparticles and could be useful for in vitro biophysics and the separation of proteins. 相似文献
Gold nanorods (AuNRs) stabilized by cetyltrimethylammonium bromide (CTAB) were synthesized and an interaction of catecholamines (CAs) with silver ions in the presence of the obtained AuNRs was studied. The reaction results into formation of core-shell Au@Ag nanorods (Au@AgNRs) and leads to a hypsochromic shift of the long-wave surface plasmon resonance (SPR) band in the absorption spectrum of AuNRs. The influence of a CA structure, excess of CTAB, interaction time, pH, concentration of AuNRs, silver ions and CAs on this interaction was studied. Based on correlation of the NRs spectral characteristics with the concentration of CAs, a method for spectrophotometric determination of dobutamine, epinephrine, norepinephrine and dopamine with detection limits 27, 18, 16 and 13 μg L−1, respectively, has been developed. The method can be applied to the analysis of medicines. 相似文献
Glassy carbon electrodes were modified with composites containing cobalt tetraaminophenoxy phthalocyanine nanoparticles (CoTAPhPc NP ), multi‐walled carbon nanotubes (MWCNT) and gold nanorods (AuNRs). The modified electrodes were studied for their electrocatalytic behavior towards the reduction of hydrogen peroxide. Phthalocyanine nanoparticles significantly improved electron transfer kinetics as compared to phthalocyanines which are not in the nanoparticle form when alone or in the presence of multiwalled carbon nanotubes (MWCNTs). CoTAPhPc NP ‐MWCNT‐GCE proved to be suitable for hydrogen peroxide detection with a catalytic rate constant of 3.45×103 M?1 s?1 and a detection limit of 1.61×10?7 M. Adsorption Gibbs free energy ΔGo was found to be ?19.22 kJ mol?1 for CoTAPhPc NP ‐MWCNT‐GCE. 相似文献
Nanoribbon‐shaped nanocomposites composed of conjugated polymer poly(3‐hexylthiophene) (P3HT) nanoribbons and plasmonic gold nanorods (AuNRs) were crafted by a co‐assembly of thiol‐terminated P3HT (P3HT‐SH) nanofibers with dodecanethiol‐coated AuNRs (AuNRs‐DDT). First, P3HT‐SH nanofibers were formed due to interchain π–π stacking. Upon the addition of AuNRs‐DDT, P3HT‐SH nanofibers were transformed into nanoribbons decorated with the aligned AuNRs on the surface (i.e., nanoribbon‐like P3HT/AuNRs nanocomposites). Depending on the surface coverage of the P3HT nanoribbons by AuNRs, these hierarchically assembled nanocomposites exhibited broadened and red‐shifted absorption bands of AuNRs in nIR region due to the plasmon coupling of adjacent aligned AuNRs and displayed quenched photoluminescence of P3HT. Such conjugated polymer/plasmonic nanorod nanocomposites may find applications in fields, such as building blocks for complex superstructures, optical biosensors, and optoelectronic devices. 相似文献
The authors report on a robust method for the synthesis of gold nanorods (AuNRs) with tunable dimensions and longitudinal surface plasmon resonance. The method relies on seed-mediated particle growth in the presence of benzalkonium chloride (BAC) in place of the widely used surfactant cetyltrimethyl ammonium bromide (CTAB). Uniform AuNRs were obtained by particle growth in solution, and BAC is found to stabilize the AuNRs for >1 year. The SERS activity of the resulting AuNRs is essentially identical to that of CTAB-protected nanorods. The SERS activity of the BAC protected nanorods was applied to the quantitative analysis of potato virus X (PVX). The calibration plot for PVX is linear in the 10 to 750 ng?mL?1 concentration range, and the detection limit is 2.2 ng?mL?1.
Graphical abstract SERS-active gold nanorods (AuNRs) have been prepared by using benzalkonium chloride as stabilization agent. Effects of chemical parameters on AuNRs have been explored and AuNRs were used in quantitative analysis of potato virus X (PVX).
Although gold nanorods (AuNRs) have strong potential applications in nanotechnology, plasmonics, and sensing, the scale-up synthesis of isolated AuNRs in gram quantities remains a challenge. Nearly all previously reported methods produce aqueous solutions of cetyltrimethylammonium bromide (CTAB)-coated AuNRs in milligram quantities with yields of approximately 20–30 % in terms of AuI to Au0 conversion. In addition, it is difficult to remove the CTAB bilayer from the surface of AuNRs and yet make them soluble and functionalized for further processing and chemical modification. This report describes the synthesis of monodisperse functionalized AuNRs (standard deviation, σ≈5 %) in gram quantities. Our approach involved increasing the concentration of HAuCl4 ⋅ 3 H2O in the growth solution to produce larger quantities of starting AuNRs and further reducing the remaining AuI ions onto the surface of AuNRs. The slow and controlled addition of ascorbic acid as a reducing agent continued the conversion of AuI into Au0 (through a disproportionation reaction) onto the surface of the nanorods, which maintained their uniform morphology without creating any unwanted impurities of various shapes. In addition, this approach significantly narrowed the size distribution owing to continuous growth of the partially grown AuNRs during the initial stage of the synthesis. To isolate a 1 g quantity of the AuNRs and to make them functionalized for further chemical reactions, a ligand-exchange approach was utilized, in which the CTAB surfactant was replaced with 4-mercaptophenol. The thiol group from 4-mercaptophenol formed a covalent bond with the surface of the AuNRs, leaving free functional OH groups available for further chemical coupling reactions. For the ligand-exchange process, a concentrated solution of 4-mercaptophenol in tetrahydrofuran solution was introduced into the AuNRs solution. Pure AuNRs functionalized with 4-mercaptophenol were isolated by dispersion and rinsing with an excess amount of THF, followed by centrifugation. 相似文献
A novel electrochemical immunoassay was developed based on the signal amplification strategy of silver deposition directed by gold nanorods (AuNRs), which was in-situ assembled on the sandwich immunocomplex. The superstructure formed by the self-assembly of AuNRs provided abundant active sites for the nucleation of silver nanoparticles. In this pathway, the stripping current of silver was greatly enhanced. Using human immunoglobulin G (HIgG) as a model analyte, the ultrasensitive immunoassay showed a wide linear range of six orders of magnitude from 0.1 fg mL−1 to 100 pg mL−1, with the low detection limit down to 0.08 fg mL−1. The practicality of this electrochemical immunoassay for detection of HIgG in serum was validated with the average recovery of 93.9%. In addition, this enzyme-free immunoassay also has the advantages of acceptable reproducibility and specificity, and thus this immunosensing protocol can be extended to the detection of other low-abundant protein biomarkers. 相似文献
A quadruple‐responsive nanocomposite that responds to temperature, pH, magnetic field, and NIR is obtained by incorporating superparamagnetic iron oxide nanoparticles (SPIONs) and gold nanorods (AuNRs) into a dextran‐based smart copolymer network. The dual‐sensitive copolymer is prepared by sequential RAFT polymerization of methacrylic acid and N‐isopropylacrylamide from trithiocarbonate groups linked to dextran in one pot. These functionalized nanocomposites with superior stability can respond to the four stimuli mentioned above well. As evidenced by UV–vis and TEM measurements, the temperature‐induced unusual blue‐shift in the longitudinal plasmon band is possibly due to the side‐to‐side assembly of AuNRs. 相似文献
In the present work a central composite design based on response surface methodology (RSM) is employed for fine tuning of the aspect ratios of seed-mediated synthesized gold nanorods (GNRs). The relations between the affecting parameters, including ratio of l-ascorbic acid to Au3+ ions, concentrations of silver nitrate, CTAB, and CTAB-capped gold seeds, were explored using a RSM model. It is observed that the effect of each parameter on the aspect ratio of developing nanorods highly depends on the value of the other parameters. The concentrations of silver ions, ascorbic acid and seeds are found to have a high contribution in controlling the aspect ratios of NRs. The optimized parameters led to a high yield synthesis of gold nanorods with an ideal aspect ratio ranging from 1 (spherical particle) to 4.9. In addition, corresponding tunable surface Plasmon absorption band has been extended to 880 nm. The resulted nanorods were characterized by UV–visible spectrometry and transmission electron microscopy. 相似文献
We report a one-step synthesis of a nanocomposite of goethite (α-FeOOH) nanorods and reduced graphene oxide (RGO) using a solution method in which ferrous cations serve as a reducing agent of graphite oxide (GO) to graphene and a precursor to grow goethite nanorods. As-prepared goethite nanorods have an average length of 200 nm and a diameter of 30 nm and are densely attached on both sides of the RGO sheets. The electrochemical properties of the nanocomposite were characterized by cyclic voltammetry (CV) and chronopotentiometry (CP) charge–discharge tests. The results showed that goethite/RGO composites have a high electrochemical capacitance of 165.5 F g?1 with an excellent recycling capability making the material promising for electrochemical capacitors. 相似文献
Facile achievement of gold nanorods (AuNRs) with controllable longitudinal surface plasmon resonance (LSPR) is of great importance for their applications in various fields. The LSPR of AuNRs is sensitive to their aspect ratio, which is still hard to be precisely tuned by direct synthesis. In this work, we report a simple approach for end-selective etching of AuNRs by a rapid oxidation process with Au(III) in cetyltrimethylammonium bromide (CTAB) solution at a mild temperature. The LSPR wavelength and the length of AuNRs blue shifted linearly as a function of the amount of Au(III), while the diameter of AuNRs remained nearly constant. The oxidative rate is temperature dependent, and the oxidative process for a desired LSPR can be accomplished within 15 min at 60 °C. Further investigations indicated that Br– determine the occurrence of the oxidation between AuNRs and Au(III), and a small amount of surfactant chain (CTA+) is crucial for stabilizing AuNRs. This method presents a quick but robust strategy for acquiring AuNRs with an arbitrary intermediate LSPR wavelength using the same starting AuNRs, and can be a powerful tool for subsequent applications. 相似文献