Investigation of natural compounds by the HPLC method II. HPLC fingerprint method for the epigeal organs of Ferula kuhistanica and F. tenuisecta

A microcolumn HPLC fingerprint method has been developed for the epigeal organs ofFerula kuhistanica andF. tenuisecta. The possibility has been shown of control by the microcolumn HPLC method of the qualitative and quantitative yields of ferutinin in the initial plant raw material and in preparations extracted from it, and also at each stage of the isolation of the desired product. The method is universal and does not depend on species and chemical composition of the plant. Institute of the Chemistry of Plant Substances, Uzbekistan Academy of Sciences, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 635–636, November–December, 1992.     

Investigation of natural compounds by the HPLC method I. Microcolumn HPLC separation of esters of carotane alcohols from the roots of Ferula tenuisecta

Reversed-phase and normal-phase variants of microcolumn high-performance liquid chromatograph (HPLC) for the separation of the preparation Teféstrol, isolated from the roots ofFerula tenuisecta have been developed. Minor components have been isolated. The amounts of the major and minor components in Tefestrol have been determined.     

Investigation of natural compounds by the HPLC method I. Microcolumn HPLC separation of esters of carotane alcohols from the roots of Ferula tenuisecta

Reversed-phase and normal-phase variants of microcolumn high-performance liquid chromatograph (HPLC) for the separation of the preparation Teféstrol, isolated from the roots ofFerula tenuisecta have been developed. Minor components have been isolated. The amounts of the major and minor components in Tefestrol have been determined.Institute of Chemistry of Plant Substances, Uzbekistan Academy of Sciences, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 632–634, November–December, 1992.     

The investigation of natural compounds by HPLC. III. A study of sesquiterpene lactones of the guaiane type by microcolumn HPLC

Two comparable methods of microcolumn high-performance liquid chromatography (HPLC) have been developed for the sesquiterpene lactones leucomicin, matricarin, austricin, and anhydroaustricin and for the drug isolated from the epigeal part ofArtemisia leucodes.     

The investigation of natural compounds by HPLC. III. A study of sesquiterpene lactones of the guaiane type by microcolumn HPLC

Two comparable methods of microcolumn high-performance liquid chromatography (HPLC) have been developed for the sesquiterpene lactones leucomicin, matricarin, austricin, and anhydroaustricin and for the drug isolated from the epigeal part ofArtemisia leucodes.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 4, pp. 526–529, July–August, 1993.     

Esters from the roots of Ferula kyzylkumica and F. karategina

Institute of Chemistry of Plant Substances, Uzbekistan Academy of Sciences, Tashkent. Botanical Garden of Moscow State University. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 301–303, March–April, 1993.     

Quality control method for commercially available wild Jujube leaf tea based on HPLC characteristic fingerprint analysis of flavonoid compounds

Flavonoids are the main active components of natural medicinal plants with many physiological functions. In this study, an HPLC fingerprinting method based on the distribution and relative amount of 11 bioactive flavonoids was established for the quality evaluation of commercially available wild Jujube leaf tea (JLT) from China. Separation of the crude flavonoid extract was achieved on a column filled with C₁₈ material with a high carbon content. The flavonoids in wild JLT were identified based on UV spectroscopy and accurate mass measurements by TOF‐MS. Twenty‐one batches of practical samples collected from different habitats were analyzed by using the developed HPLC method to construct the HPLC characteristic fingerprint of wild JLT. Then, combined with clustering and similarity analyses, the HPLC characteristic fingerprint was used for the authentication and quality evaluation of commercial wild JLT. Results indicated that the proposed HPLC characteristic fingerprint reflected the inherent characteristics of wild JLT collected from different regions. Authenticity identification and quality control of commercially available wild Jujube tea were achieved based on the HPLC characteristic fingerprint analysis. This new approach to bioactive component profiling provided a promising reference method for the quality evaluation of commercial wild flower and plant tea.