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Dinuclear Ruthenium(II) Complexes as Two‐Photon,Time‐Resolved Emission Microscopy Probes for Cellular DNA 下载免费PDF全文
Elizabeth Baggaley Martin R. Gill Nicola H. Green David Turton Igor V. Sazanovich Stanley W. Botchway Carl Smythe John W. Haycock Julia A. Weinstein Jim A. Thomas 《Angewandte Chemie (International ed. in English)》2014,53(13):3367-3371
The first transition‐metal complex‐based two‐photon absorbing luminescence lifetime probes for cellular DNA are presented. This allows cell imaging of DNA free from endogenous fluorophores and potentially facilitates deep tissue imaging. In this initial study, ruthenium(II) luminophores are used as phosphorescent lifetime imaging microscopy (PLIM) probes for nuclear DNA in both live and fixed cells. The DNA‐bound probes display characteristic emission lifetimes of more than 160 ns, while shorter‐lived cytoplasmic emission is also observed. These timescales are orders of magnitude longer than conventional FLIM, leading to previously unattainable levels of sensitivity, and autofluorescence‐free imaging. 相似文献
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Elizabeth Baggaley Martin R. Gill Nicola H. Green David Turton Igor V. Sazanovich Stanley W. Botchway Carl Smythe John W. Haycock Julia A. Weinstein Jim A. Thomas 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2014,126(13):3435-3439
The first transition‐metal complex‐based two‐photon absorbing luminescence lifetime probes for cellular DNA are presented. This allows cell imaging of DNA free from endogenous fluorophores and potentially facilitates deep tissue imaging. In this initial study, ruthenium(II) luminophores are used as phosphorescent lifetime imaging microscopy (PLIM) probes for nuclear DNA in both live and fixed cells. The DNA‐bound probes display characteristic emission lifetimes of more than 160 ns, while shorter‐lived cytoplasmic emission is also observed. These timescales are orders of magnitude longer than conventional FLIM, leading to previously unattainable levels of sensitivity, and autofluorescence‐free imaging. 相似文献
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Lipophilic Tetranuclear Ruthenium(II) Complexes as Two‐Photon Luminescent Tracking Non‐Viral Gene Vectors 下载免费PDF全文
Dr. Bole Yu Cheng Ouyang Kangqiang Qiu Prof. Jing Zhao Prof. Liangnian Ji Prof. Hui Chao 《Chemistry (Weinheim an der Bergstrasse, Germany)》2015,21(9):3691-3700
Fluorescence detection is the most effective tool for tracking gene delivery in living cells. To reduce photodamage and autofluorescence and to increase deep penetration into cells, choosing appropriate fluorophores that are capable of two‐photon activation under irradiation in the NIR or IR regions is an effective approach. In this work, we have developed six tetranuclear ruthenium(II) complexes, GV1–6 , and have studied their one‐ and two‐photon luminescence properties. DNA interaction studies have demonstrated that GV2–6 , bearing hydrophobic alkyl ether chains, show more efficient DNA condensing ability but lower DNA binding constants than GV1 . However, the hydrophobic alkyl ether chains also enhance the DNA delivery ability of GV2–6 compared with that of GV1 . More importantly, we have applied GV1–6 as non‐viral gene vectors for tracking DNA delivery in living cells by one‐ and two‐photon fluorescence microscopies. In two‐photon microscopy, a high signal‐to‐noise contrast was achieved by irradiation with an 830 nm laser. This is the first example of the use of transition‐metal complexes for two‐photon luminescent tracking of the cellular pathways of gene delivery and as DNA carriers. Our work provides new insights into improving real‐time tracking during gene delivery and transfection as well as important information for the design of multifunctional non‐viral vectors. 相似文献
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Prof. Kevin D. Belfield Dr. Mykhailo V. Bondar Alma R. Morales Xiling Yue Dr. Gheorghe Luchita Dr. Olga V. Przhonska Dr. Olexy D. Kachkovsky 《Chemphyschem》2012,13(15):3481-3491
The synthesis, comprehensive linear photophysical characterization, two‐photon absorption (2PA), steady‐state and time‐resolved stimulated emission depletion properties of a new fluorene derivative, (E)‐1‐(2‐(di‐p‐tolylamino)‐9,9‐diethyl‐9H‐fluoren‐7‐yl)‐3‐(thiophen‐2‐yl)prop‐2‐en‐1‐one ( 1 ), are reported. The primary linear spectral properties, including excitation anisotropy, fluorescence lifetimes, and photostability, were investigated in a number of aprotic solvents at room temperature. The degenerate 2PA spectra of 1 were obtained with open‐aperture Z‐scan and two‐photon induced fluorescence methods, using a 1 kHz femtosecond laser system, and maximum 2PA cross‐sections of ~400–600 GM were obtained. The nature of the electronic absorption processes in 1 was investigated by DFT‐based quantum chemical methods implemented in the Gaussian 09 program. The one‐ and two‐photon stimulated emission spectra of 1 were measured over a broad spectral range using a femtosecond pump–probe‐based fluorescence quenching technique, while a new methodology for time‐resolved fluorescence emission spectroscopy is proposed. An effective application of 1 in fluorescence bioimaging was demonstrated by means of one‐ and two‐photon fluorescence microscopy images of HCT 116 cells containing dye encapsulated micelles. 相似文献
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Back Cover: Three‐Dimensional Protein Networks Assembled by Two‐Photon Activation (Angew. Chem. Int. Ed. 22/2014) 下载免费PDF全文
Dr. Volker Gatterdam Dr. Radhan Ramadass Dr. Tatjana Stoess Manuela A. H. Fichte Prof. Dr. Josef Wachtveitl Prof. Dr. Alexander Heckel Prof. Dr. Robert Tampé 《Angewandte Chemie (International ed. in English)》2014,53(22):5712-5712
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Two‐photon microscopy (TPM) has become an indispensible tool in biology and medicine owing to the capability of imaging the intact tissue for a long period of time. To make it a versatile tool in biology, a variety of two‐photon probes for specific applications are needed. In this context, many research groups are developing two‐photon probes for various applications. In this Focus Review, we summarize recent results on model studies and selected examples of two‐photon probes that can detect intracellular free metal ions in live cells and tissues to provide a guideline for the design of useful two‐photon probes for various in vivo imaging applications. 相似文献
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Pingyu Zhang Dr. Lingmin Pei Dr. Yu Chen Wenchao Xu Qitian Lin Jinquan Wang Jingheng Wu Dr. Yong Shen Prof. Liangnian Ji Prof. Hui Chao 《Chemistry (Weinheim an der Bergstrasse, Germany)》2013,19(46):15494-15503
A new dinuclear RuII polypyridyl complex, [(bpy)2Ru(H2bpip)Ru(bpy)2]4+ ( RuH2bpip , bpy=2,2‐bipyridine, H2bpip=2,6‐pyridyl(imidazo[4,5‐f][1,10]phenanthroline), was developed to act as a one‐ and two‐photon luminescent probe for biological Cu2+ detection. This RuII complex shows a significant two‐photon absorption cross section (400 GM) and displays a remarkable one‐ and two‐photon luminescence switch in the presence of Cu2+ ions. Importantly, RuH2bpip can selectively recognise Cu2+ in aqueous media in the presence of other abundant cellular cations (such as Na+, K+, Mg2+, and Ca2+), trace metal ions in organisms (such as Zn2+, Ag+, Fe3+, Fe2+, Ni2+, Mn2+, and Co2+), prevalent toxic metal ions in the environment (such as Cd2+, Hg2+, and Cr3+), and amino acids, with high sensitivity (detection limit≤3.33×10?8 M ) and a rapid response time (≤15 s). The biological applications of RuH2bpip were also evaluated and it was found to exhibit low cytotoxicity, good water solubility, and membrane permeability; RuH2bpip was, therefore, employed as a sensing probe for the detection of Cu2+ in living cells and zebrafish. 相似文献
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A series of Zn2+‐selective two‐photon fluorescent probes (AZnM1−AZnN) that had a wide range of dissociation constants (KdTP=8 nm‐ 12 μM ) were synthesized. These probes showed appreciable water solubility (>3 μM ), cell permeability, high photostability, pH insensitivity at pH>7, significant two‐photon action cross‐sections (86–110 GM) upon complexation with Zn2+, and can detect the Zn2+ ions in HeLa cells and in living tissue slices of rat hippocampal at a depth of >80 μm without mistargeting and photobleaching problems. These probes can potentially find application in the detection of various amounts of Zn2+ ions in live cells and intact tissues. 相似文献
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Cyclometalated Iridium(III) Complexes as Two‐Photon Phosphorescent Probes for Specific Mitochondrial Dynamics Tracking in Living Cells 下载免费PDF全文
Chengzhi Jin Jiangping Liu Dr. Yu Chen Leli Zeng Ruilin Guan Cheng Ouyang Prof. Liangnian Ji Prof. Dr. Hui Chao 《Chemistry (Weinheim an der Bergstrasse, Germany)》2015,21(34):12000-12010
Five cyclometalated iridium(III) complexes with 2‐phenylimidazo[4,5‐f][1,10]phenanthroline derivatives ( IrL1 – IrL5 ) were synthesized and developed to image and track mitochondria in living cells under two‐photon (750 nm) excitation, with two‐photon absorption cross‐sections of 48.8–65.5 GM at 750 nm. Confocal microscopy and inductive coupled plasma‐mass spectrometry (ICP‐MS) demonstrated that these complexes selectively accumulate in mitochondria within 5 min, without needing additional reagents for membrane permeabilization, or replacement of the culture medium. In addition, photobleaching experiments and luminescence measurements confirmed the photostability of these complexes under continuous laser irradiation and physiological pH resistance. Moreover, results using 3D multicellular spheroids demonstrate the proficiency of these two‐photon luminescent complexes in deep penetration imaging. Two‐photon excitation using such novel complexes of iridium(III) for exclusive visualization of mitochondria in living cells may substantially enhance practical applications of bioimaging and tracking. 相似文献
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Cover Picture: Two‐Photon Microscopy and Spectroscopy of Lanthanide Bioprobes (ChemPhysChem 14/2007)
Anthony D'Aléo Dr. Guillaume Pompidor Bénédicte Elena Dr. Jean Vicat Prof. Patrice L. Baldeck Dr. Loïc Toupet Dr. Richard Kahn Dr. Chantal Andraud Dr. Olivier Maury Dr. 《Chemphyschem》2007,8(14):2025-2025
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Back Cover: Light‐Induced Protein Dimerization by One‐ and Two‐Photon Activation of Gibberellic Acid Derivatives in Living Cells (Angew. Chem. Int. Ed. 9/2015) 下载免费PDF全文
M. Sc. Korwin M. Schelkle B. Sc. Tristan Griesbaum M. Sc. Dirk Ollech Dipl.‐Chem. Steffy Becht Dr. Tiago Buckup Dr. Manuel Hamburger Dr. Richard Wombacher 《Angewandte Chemie (International ed. in English)》2015,54(9):2856-2856
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Dr. Li Xu Xiang Chen Jingheng Wu Jinquan Wang Prof. Liangnian Ji Prof. Dr. Hui Chao 《Chemistry (Weinheim an der Bergstrasse, Germany)》2015,21(10):4008-4020
A series of dinuclear ruthenium(II) complexes were synthesised, and the complexes were determined to be new highly selective compounds for binding to telomeric G‐quadruplex DNA. The interactions of these complexes with telomeric G‐quadruplex DNA were studied by using circular dichroism (CD) spectroscopy, fluorescence resonance energy transfer (FRET) melting assays, isothermal titration calorimetry (ITC) and molecular modelling. The results showed that the complexes 1 , 2 and 4 induced and stabilised the formation of antiparallel G‐quadruplexes of telomeric DNA in the absence of salt or in the presence of 100 mM K+‐containing buffer. Furthermore, complexes 1 and 2 strongly bind to and effectively stabilise the telomeric G‐quadruplex structure and have significant selectivity for G‐quadruplex over duplex DNA. In comparison, complex 3 had a much lesser effect on the G‐quadruplex, suggesting that possession of a suitably sized plane for good π–π stacking with the G‐quadruplets is essential for the interaction of the dinuclear ruthenium(II) complexes with the G‐quadruplex. Moreover, telomerase inhibition by the four complexes and their cellular effects were studied, and complex 1 was determined to be the most promising inhibitor of both telomerase and HeLa cell proliferation. 相似文献
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Cover Picture: An Efficient and Modular Route to Sequence‐Defined Polymers Appended to DNA (Angew. Chem. Int. Ed. 18/2014) 下载免费PDF全文
Thomas G. W. Edwardson Dr. Karina M. M. Carneiro Dr. Christopher J. Serpell Prof. Hanadi F. Sleiman 《Angewandte Chemie (International ed. in English)》2014,53(18):4497-4497
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Frontispiece: Cyclometalated Iridium(III) Complexes as Two‐Photon Phosphorescent Probes for Specific Mitochondrial Dynamics Tracking in Living Cells 下载免费PDF全文
Chengzhi Jin Jiangping Liu Dr. Yu Chen Leli Zeng Ruilin Guan Cheng Ouyang Prof. Liangnian Ji Prof. Dr. Hui Chao 《Chemistry (Weinheim an der Bergstrasse, Germany)》2015,21(34)