Study on naringenin-CTMAB-DNA system by resonance light scattering technique and its analytical application

A new high-sensitivity determination method of deoxyribonucleic acid (DNA) with detection limit at nanogram levels was proposed. Based on the measurement of resonance light scattering (RLS), it was found DNA could combine with naringenin and cetyltrimethylammonium bromide (CTMAB) in basic Tris-HCl buffer and produce enhanced RLS signal. The optimum conditions for this system were studied in detail. The enhanced intensity of RLS of naringenin-CTMAB at 353 nm was directly proportional to the concentration of DNA in the range of 0.017-1.7 μg mL(-1). The detection limit was 5.06 ng mL(-1). Using the proposed method, the synthetic samples were analyzed with satisfactory results, the recovery was 99.3-105.0% and RSD was 0.7-3.7%.     

Study on the interaction of nucleic acids with silver nanoparticles—Al(III) by resonance light scattering technique and its analytical application

It is found that Al(III) can further enhance the intensity of resonance light scattering (RLS) of the silver nanoparticles (AgNPs) and nucleic acids system. Based on this, a novel method of determination of nucleic acids is proposed in this paper. Under optimum conditions, there are linear relationships between the enhancing extent of RLS and the concentration of nucleic acids in the range of 1.0 × 10⁻⁹-1.0 × 10⁻⁷ g mL⁻¹, 1.0 × 10⁻⁷-2.0 × 10⁻⁶ g mL⁻¹ for fish sperm DNA (fsDNA), 1.0 × 10⁻⁹-7.0 × 10⁻⁸ g mL⁻¹ for calf thymus DNA (ctDNA) and 1.0 × 10⁻⁹-1.0 × 10⁻⁷ g mL⁻¹ for yeast RNA (yRNA). The detection limits (S/N = 3) of fsDNA, ctDNA and yRNA are 4.1 × 10⁻¹⁰ g mL⁻¹, 4.0 × 10⁻¹⁰ g mL⁻¹ and 4.5 × 10⁻¹⁰ g mL⁻¹, respectively. The studies indicate that the RLS enhancement effect should be ascribed to the formation of AgNPs-Al(III)-DNA aggregations through electrostatic attraction and adsorption bridging action of Al(III). And the sensitivity and stability of the AgNPs-fsDNA system could be enhanced by Al(III).     

共振光散射光谱研究噻嗪红R与牛血清白蛋白的作用及其分析应用

研究了噻嗪红R(Thiazine red R,TR)与牛血清蛋白(BSA)作用的共振光散射(RLS)光谱特征。考察了各种影响因素,并计算出了BSA与TR的结合比(质量之比)为1.25。结果表明,在优化条件下体系的RLS强度与蛋白质浓度在一定范围内具有良好的线性关系,据此建立了一种蛋白质测定的新方法。在最佳实验条件(TR,2.0×10-5mol/L;pH2.36)下,BSA的线性范围是0.01~5.0μg/mL,检出限为1.4ng/mL。该方法已用于合成样品及尿液的测定。     

盐酸雷尼替丁的共振光散射新体系研究及其分析应用

在酸性条件下,盐酸雷尼替丁、铬黑T和钼酸铵通过静电作用形成三元离子缔合物,使体系的共振光散射明显增强.据此建立了共振光散射测定盐酸雷尼替丁的新方法.在最佳条件下,体系的最大散射峰位于363 nm处.共振光散射增强的程度与盐酸雷尼替丁的浓度呈良好的线性关系.方法的线性范围在0.015～0.165 mg/mL,检出限为9.5×10-3 mg/mL.将该方法用于市售盐酸雷尼替丁片的测定,并与药典方法进行对照,证明两种方法之间无显著性差异.     

A study on the interaction between concanavalin A and glycogen by light scattering technique and its analytical application

The mixture of concanavalin A (Con A) and glycogen shows strong light scattering character. Based on it, the interaction between Con A and glycogen was studied on a common spectrofluorimeter by light scattering technique. Many factors affecting the light scattering intensity (LSI), such as pH, temperature, reaction time, ion strength and the denaturing agent of protein were studied in detail. Experimental results showed that the LSI reached its maximum after mixing Con A with glycogen for about 20 min in pH 7.4 Tris-HCl buffer at 37 degrees C. The results also suggested that the conformation of Con A was critical for its unique binding affinity to glycogen. Electrostatic forces should not be the primary interaction between glycogen and Con A. Under proper experimental conditions, the determination method for glycogen by light scattering technique was developed. The glycogen determination can be performed in the range of 0.48-32.0, 0.50-32.0 and 0.32-24.0 mug/ml for Rabbit liver glycogen (RL Gly), Oyster glycogen (O Gly) and Clam Glycogen (C Gly), respectively. The influence of co-existing substances such as proteins, mono- and di-saccharides and metal ions was evaluated, and little interference came from the foreign substances. The determinations of glycogen in synthetic samples demonstrated that the recovery rate was in the range of 98.1-103% and the relative standard deviations (RSD) were lower than 5.0%.     

Ru（bpy）3^2＋ -DNA体系的共振光散射光谱研究及其分析应用

研究了Ru(bpy)32 与脱氧核糖核酸(DNA)作用的共振光散射光谱。基于DNA对Ru(bpy)32 共振光散射的增强效应,建立了共振光散射法测定DNA的新方法。在最佳实验条件下,Ru(bpy)32 在373nm处的共振光散射增强与DNA的质量浓度呈线性关系,线性范围为0.04~3.2μg/mL,检出限为16ng/mL。应用于合成样品及实际样品中DNA的测定。     

偶氮胭脂红G共振光散射法测定羟自由基的研究及应用

基于Fenton反应产生的·OH对偶氮胭脂红G(AG)的褪色反应,使偶氮胭脂红G在434 nm处共振散射光强度降低,采用偶氮胭脂红G为指示剂,在优化测定条件后,建立了一个共振光散射法测定Fenton反应所产生的·OH的新方法,并用所建立的新方法研究了一些常见抗氧化剂对·OH清除的量效关系,验证了方法的可行性.在此基础上,用该方法研究了一些茶叶水提取液的抗氧化能力,结果表明,所有茶叶提取液均具有一定清除·OH的能力.在所研究的几种茶叶中,以普洱茶清除·OH的能力最强.     

甲基紫与苋菜红相互作用的双波长共振光散射比率光谱研究及其分析应用

应用双波长共振光散射比率法(DW-RLS)研究了甲基紫与苋菜红之间的相互作用.在pH 1.24的乙酸钠-HCl缓冲溶液中,甲基紫和苋菜红本身的共振光散射(RLS)信号均很弱,但是当它们相互作用形成缔合物时,导致RLS信号明显增强并出现新的RLS光谱,适当浓度的Triton X-100存在使结合反应敏化,缔合物最大散射峰位于528 nm,RLS信号强度与苋菜红的浓度呈线性关系.通过测量528 nm处的RLS强度或两个波长处RLS强度比值(I417/I343),可对苋菜红进行定量检测.当溶液中甲基紫的浓度为1.54×10-5 mol/L时,RLS法测定苋菜红的线性范围和检出限分别为0.05～0.50 μg/mL和0.02 μg/mL,而DW-RLS法的线性范围和检出限分别为0.01～0.60 μg/mL和1 ng/mL,与RLS法相比较,DW-RLS法受酸度、离子强度等环境条件影响较小,并且有更宽的线性范围和更低的检出限.     

盐酸奎宁与全氟辛烷磺酸体系的共振光散射光谱研究及其分析应用

研究了盐酸奎宁(Quinine dihydrochloride,简称Quinine)与全氟辛烷磺酸(perfluorooctane sulfonate,简称PFOS)相互作用的共振光散射(resonance light scattering,RLS)光谱,并建立了PFOS的共振光散射分析方法.在pH值为2.87的Britton-Robinson(BR)缓冲溶液中,全氟辛烷磺酸根阴离子与质子化的盐酸奎宁通过静电引力和疏水作用形成2:1的离子缔合物,引起共振光散射强度(IRLS)显著增强,最大散射波长位于283nm处,增强的散射信号强度与PFOS浓度在0.10～50.0μmol/L范围内呈线性关系,据此建立了测定PFOS的散射分析方法,检测限为9.88nmol/L.讨论了体系的最佳反应条件及外来物质的干扰,同时研究了体系的吸收光谱及荧光光谱,并探讨了反应机理.本方法用于水样及人体血清样品中PFOS的测定,RSD≤4.2%.     

Determination of deoxyribonucleic acids by a resonance light scattering technique and its application

For the first time, acetamiprid has been used to determine nucleic acid (DNA) using the resonance light scattering (RLS). The RLS of acetamiprid was greatly enhanced by DNA in the range of pH 1.6-1.8. A RLS peak at 313 nm was found, and the enhanced intensity of RLS at this wavelength was proportional to the concentration of DNA. The linear range of the calibration curve was 0-11.0 microg ml(-1) with the detection limit of 20 ng ml(-1). The nucleic acids in synthetic sample and in rice seedling extraction were determined satisfactorily. The interaction mechanism of acetamiprid and DNA is discussed. Mechanism studies show that the enhanced RLS is due to the aggregation of acetamiprid in the presence of DNA.     

Study on the interaction of trypsin with some DNAs and their analytical application by resonance Rayleigh scattering spectra

When trypsin reacts with Herring sperm DNA (hsDNA), Salmon sperm DNA (sDNA), and Calf thymus DNA (ctDNA) to form a complex, the resonance Rayleigh scattering (RRS) was remarkably enhanced and new RRS spectra appear. These new spectra have similar characteristics of RRS spectra. The maximum RRS peaks are at 307 nm (hsDNA, sDNA) and 290 nm (ctDNA), and other peaks are at 350 nm. The scattering intensity is proportional to the concentration of DNA or trypsin; so this intereaction can be used to determine trypsin using DNA or DNA using trypsin. In the determination of DNA using trypsin, the linear ranges for hsDNA, sDNA, and ctDNA are 0–2.3, 0–2.5, and 0–1.9 μg·mL⁻¹, and the detection limits are 0.4, 0.7, and 1.1 ng·mL⁻¹, respectively. In the determination of trypsin using hsDNA, the linear range is 0–30.0 μg·mL⁻¹, and the detection limit is 39.0 ng·mL⁻¹. In this paper, the intereaction conditions were optimized. The affecting factors, chemical properties of the complex, and the composition ratio of trypsin with DNA were investigated. Using trypsin as RRS probe, a sensitive method for the determination of trace amounts of DNA was developed. Translated from Chemical Journal of Chinese Universities, 2006, 27(3) (in Chinese)     

Resonance light scattering spectroscopy study of interaction between norfloxacin and calf thymus DNA and its analytical application

The interaction between norfloxacin and calf thymus double-stranded DNA (dsDNA) has been studied by a resonance light scattering (RLS) technique with a common spectrofluorometer. The characteristics of RLS spectra, the effective factors and optimum conditions of the reaction have been investigated. In Britton-Robinson (BR) buffer (pH 5.87), norfloxacin has a maximum peak 405.5 nm and the RLS intensity is remarkably enhanced by trace amount of calf thymus dsDNA due to the interaction between norfloxacin and dsDNA. The binding of norfloxacin to DNA forms large particles, which were characterized by RLS spectrum, scanning electron microscopy (SEM), ultraviolet-visible (UV-vis) spectrum, and fluorescence spectrum. Based on the enhanced RLS intensity, a novel method for sensitive determination of calf thymus dsDNA concentration ranging from 0.02 to 2.3 microg ml(-1) was developed. The determination limit (3 sigma) was 1.2 ng ml(-1). The method is simple, rapid, practical and relatively free from interference generated by coexisting substance, as well as much more sensitive than most of the reported methods. Three synthetic samples of ctDNA were determined with satisfactory results.     

Investigation on the interaction between anthracyclines and DNA in the presence of paclitaxel by resonance light scattering technique

We have developed a method to investigate the interaction between DNA-targeted anthracyclines and DNA in the presence of the drug paclitaxel. It is based on resonance light scattering (RLS) and on the finding that anthracyclines when bound to DNA undergo a dramatic enhancement in their RLS intensities, while paclitaxel does not display such an effect. However, the RLS intensities of the anthracyclines-DNA associates are remarkably enhanced again on addition of paclitaxel. UV-visible spectra reveal interactions between paclitaxel and anthracyclines, but no reaction between paclitaxel and DNA. Consequently, paclitaxel, though not DNA-targeted, can improve the DNA-binding capabilities of anthracyclines. Binding constants between anthracyclines and DNA, and improved efficiency of paclitaxel on the DNA-binding capabilities of anthracyclines were calculated. The DNA binding constants of doxorubicin, epirubicin, and mitoxantrone, respectively, are 4.53?×?10⁵?L?mol^?1, 6.05?×?10⁵?L?mol^?1, and 9.47?×?10⁵?L?mol^?1. The improved values in presence of paclitaxel are 78%, 47% and 19%. We also have investigated the effects of drug concentrations and the order of adding the drugs. Displacement studies (using methylene blue as a competitive agent) provided additional information on the mechanisms of the interaction between paclitaxel and anthracyclines.

The resonance Rayleigh scattering of the interaction of copper(II)-bleomycinA2 with DNA and its analytical application

The forming of bleomycinA2-Cu(II) cationic chelate and the interaction of the chelate with DNA have been investigated by using resonance Rayleigh scattering (RRS), molecular absorption and fluorescence spectra. The result shows that in aqueous solution, bleomycinA2 (BLMA2) can react with Cu(II) to form 1:1 cationic chelate which contributes to the changes of the absorption spectra and the quenched fluorescence of BLMA2. When the cationic chelate further bound with DNA to form ternary ion-association complexes, the remarkable enhancement of the RRS intensity was observed. In this work, the optimum conditions for the coordination reaction of BLMA2 with Cu(II) and some influencing factors have been investigated. The reaction mechanism of BLMA2-Cu(II) binding with DNA was suggested and a binding model was proposed. In addition, the fluorescence quenching type of BLMA2 was investigated. A highly sensitive, simple and rapid new method for the determination of DNA by using BLMA2-Cu(II) as RRS probe has been developed. The detection limits (3σ) are 7.2 ng/mL for ctDNA, 7.1 ng/mL for sDNA and 18 ng/mL for hsDNA. The method can be applied to the determination of trace amounts of DNA.     

功能性Cu-PVK纳米微球与核酸相互作用的共振光散射光谱及分析应用

在超声辐射下,H2O2氧化降解聚乙烯醇（PVA）,制得与Cu^2＋形成配位体的纳米尺寸的β-二酮型高分子微球（Cu-PVK）。对Cu-PVK的共振散射光（RLS）性质研究发现Cu-PVK与核酸形成缔合物时将导致Cu-PVK本身RLS信号急剧增加,基于此建立一种用RLS信号测定痕量核酸的新方法。方法的抗干扰能力强,已用于合成样品的分析。     

虎红-牛血清白蛋白共振光散射光谱研究及其分析应用

基于在pH3.0的BR缓冲溶液中蛋白质的加入使虎红的共振光散射信号增强,建立了一种蛋白质测定的新方法.详细地探讨了pH、染料用量、表面活性剂、离子强度、共存物质等对信号的影响.牛血清白蛋白浓度在0～4.0μg/mL范围内与体系散射强度呈线性关系,检出限为0.01718μg/mL.该方法用于合成样品中牛血清白蛋白的测定,...     

A sensitive resveratrol assay with a simple probe methylene blue by resonance light scattering technique

A novel resonance light scattering (RLS) method was developed for the determination of resveratrol based on the interaction between resveratrol and methylene blue (MB). It was found that at pH 8.69, the weak RLS intensity of MB was remarkably enhanced by the addition of trace amount of resveratrol with the maximum peak located at 385.0 nm. Under the optimum conditions, a good linear relationship between the enhanced RLS intensities and the concentrations of resveratrol was obtained over the range of 2.0-14.0 μg ml(-1) with the detection limit (3σ) of 0.63 μg ml(-1). The results of the analysis of resveratrol in synthetic samples and human urine are satisfactory, which showed it may provide a more sensitive, convenient, rapid and reproducible method for the detection of resveratrol, especially in biological and pharmaceutical field. In this work, the characteristics of RLS, absorption and fluorescence spectra of the resveratrol-MB system, the influencing factors and the optimum conditions of the reaction were investigated.     

Study on the interaction between torasemide and 12-tungstophosphoric acid by resonance Rayleigh scattering and resonance nonlinear scattering spectra and its analytical applications

In pH 0.6-1.1 HCl-NaAc buffer solution, torasemide (TOR) reacted with TP to form a 3:1 ion-association complexes. As a result, not only the absorption spectra were changed, but also the intensities of resonance Rayleigh scattering (RRS), second-order scattering (SOS) and frequency doubling scattering (FDS) were enhanced greatly. The maximum RRS, SOS and FDS wavelengths were located at 370, 333, 776 nm, respectively. Under given conditions, the intensities of RRS, SOS and FDS were all directly proportional to the concentration of TOR. The detection limits of RRS, SOS and FDS were 0.7173 ng mL(-1), 7.007 ng mL(-1) and 10.90 ng mL(-1). The optimum conditions and the effects of coexisting substances on the reaction were investigated. The results showed that the method had good selectivity. Therefore, a highly sensitive, simple and quick method has been developed for the determination of TOR. The method can be applied satisfactorily to the determination of TOR in tablets and urine samples.     

曲利本蓝与阿米卡星相互作用的共振瑞利散射光谱及其应用

硫酸阿米卡星(AMK)是一种氨基糖苷类抗生素,对多数肠杆菌科细菌、铜绿假单胞菌及其他假单胞菌、不动杆菌属、产碱杆菌属等有良好作用,虽然其抗菌活性较庆大霉素略低,但其突出的优点是对许多肠道革兰阴性杆菌所产生的氨基糖苷类钝化酶稳定,不会为此类酶钝化而失去抗菌活性.     

十二烷基苯磺酸钠与奎尼丁作用的共振光散射研究

研究了表面活性剂十二烷基苯磺酸钠(SDBS)与奎尼丁作用的共振散射光谱特征.实验结果表明在pH1.81的水溶液中,奎尼丁与SDBS作用并产生以278.0 nm和377.0 nm为特征峰的共振散射增强光谱,在上述特征波长下测定的增强共振光散射强度与奎尼丁浓度在一定范围内呈线性关系.当SDBS的浓度为3.0×10-4moL/L时,奎尼丁的检测限可分别达6.71 nmol/L和6.15 nmol/L.据此建立了痕量奎尼丁的共振光散射分析方法.