Chromatographic Analysis of New Antidepressant Drugs by Normal- and Reversed-Phase TLC

JPC – Journal of Planar Chromatography – Modern TLC - The selectivity of the TLC separation of six new antidepressant substances has been investigated on silanized silica gel C18 and on...     

Separation of Statin-Type Antihyperlipidemic Drugs by Reversed-Phase TLC

JPC – Journal of Planar Chromatography – Modern TLC -     

Comparison of Normal and Reversed-Phase TLC for Separation of Selected Pesticides

JPC – Journal of Planar Chromatography – Modern TLC - Fifteen urea pesticides have been separated on RP-18WF254 plates with methanol-water and mixed organic (acetonitrile-methanol, 1:1...     

Normal- and Reversed-Phase Thin-Layer Chromatography of Selected Calixarenes and Pyrene

JPC – Journal of Planar Chromatography – Modern TLC - The chromatographic behavior of calix[4]arene, 4-tert-butyl-calix[4]arene tribenzoate, 4-tert-butylcalix[5]arene, and pyrene have...     

Normal- and Reversed-Phase Thin-Layer Chromatography of Seven Oral Antidiabetic Agents

JPC – Journal of Planar Chromatography – Modern TLC - The chromatographic behavior of seven oral antidiabetic drugs -chlorpropamide, tolbutamide, glibenclamide, metformin, pioglitazone,...     

Analysis of Non-Selective Calcium-Channel Blockers by Reversed-Phase TLC

The selectivity of TLC separation of non-selective calcium-channel blockers prenylamine, lidoflazine, bepridil, and fendiline has been investigated silanized silica gel RP8 and RP18 plates. Optimization of retention and selectivity for these compounds was achieved by altering the pH and the concentration of organic modifier (methanol, ethanol, tetrahydrofuran, acetonitrile) in the aqueous mobile phases. The substances were separated in horizontal chambers and the drugs were detected by videoscanning and illumination of the plates at λ = 254 nm. On RP8 plates the best separation was achieved with 50% acetonitrile in pH 2.06 phosphate buffer as mobile phase. On RP18 the best separation was achieved with 50% ethanol in pH 2.06 phosphate buffer.

     

Normal- and Reversed-Phase Behaviour of 16,17-Secoestrone Derivatives on Cyanopropyl-Bonded Silica Gel

The retention behaviour of a number of 16,17-secoestrone derivatives has been studied by LC and HPTLC on a polar cyanopropyl-bonded stationary phase using non-aqueous and aqueous-organic mobile phases. The retention behaviour has been discussed in terms of nature of the solute, eluent and stationary phase. The correlation between retention constants of 16,17-secoestrone derivatives obtained from reversed-phases and commercially available ACD log P software (Advanced Chemistry, Toronto, Canada) has also been examined.

     

Normal- and Reversed-Phase Behaviour of 16,17-Secoestrone Derivatives on Cyanopropyl-Bonded Silica Gel

The retention behaviour of a number of 16,17-secoestrone derivatives has been studied by LC and HPTLC on a polar cyanopropyl-bonded stationary phase using non-aqueous and aqueous-organic mobile phases. The retention behaviour has been discussed in terms of nature of the solute, eluent and stationary phase. The correlation between retention constants of 16,17-secoestrone derivatives obtained from reversed-phases and commercially available ACD log P software (Advanced Chemistry, Toronto, Canada) has also been examined.     

Separation of Metalloporphyrins by TLC

JPC – Journal of Planar Chromatography – Modern TLC - Complexes of hydroxy- and alkyloxytetraphenylporphyrins with Cu (II), Zn (II), and Ni (II) have been prepared and their...     

反相毛细管电色谱分离山酮类化合物

目前,毛细管电色谱（CEC）在中草药分析中显示出一些独特的优势,如：CEC流动相相对简单、用量少、峰容量大、更易与质谱（MS）联用。特别是植物提取物中活性成分复杂,需要对色谱峰进行精确的指认并控制峰纯度;采用CEC—MS方法可在线提供中药中各种化合物的质谱图。此外,有许多天然产物在中药中的含量极低,MS的高灵敏度可弥补紫外检测器灵敏度较低的缺陷。因此,研究CEC分离中药成分的选择性问题具有重要的实际意义。蝉翼藤（Securida cainappendiculata Hassk．）为远志科远志族蝉翼藤属攀援灌木,其根茎叶有多种药效,其化学成分主要为黄酮、山酮（XAN）和皂甙。从蝉翼藤中已分离出的10个XAN类成分（结构见图1）。具有显著的抗氧化和保护神经细胞作用。我们曾用毛细管区带电泳（CZE）、高效液相色谱（HPLC）、毛细管胶束电动色谱（MEKC）和毛细管微乳电动色谱（MEEKC）对这10个XAN进行分离,并就电泳条件的变化对分离选择性的影响进行系统研究。本文采用CEC法对这10个XAN成分进行分离,并对其色谱分离条件进行优化研究,以期为今后CEC与MS联用分析中药成分打下基础。     

Normal- and Reversed-Phase Thin-Layer Chromatography of Three Structurally Related Organophosphorus Pesticides of Forensic Importance

JPC – Journal of Planar Chromatography – Modern TLC - Recently, considerable numbers of poisoning cases have been reported owing to the ingestion of three structurally related...     

Normal and Reversed-Phase TLC Determination of Rosmarinic and Valerenic Acids

JPC – Journal of Planar Chromatography – Modern TLC - The chromatographic properties of valerenic and rosmarinic acids have been studied by normal- and reversed-phase TLC with eight...     

Separation of Multiple Ferritin Subunit Types by Reversed-Phase HPLC

Abstract

Subjection of ferritin subunits to reversed-phase HPLC effected a separation of them into three chromatographic species. These three putative subunit species were found in varying proportions from porcine spleen, liver, and heart ferritins as well as human liver, spleen, and placental ferritins. Detailed chemical-physical characterization of each of the isolated species indicate them to be two H-type and one L-type ferritin subunits. Thus, reversed-phase HPLC presently stands as the only available means to separate preparatively the various subunit types of ferritin.     

Separation of Bioactive Prostaglandins and their Metabolites by Reversed-Phase Thin-Layer Chromatography

JPC – Journal of Planar Chromatography – Modern TLC - Prostaglandins are biological lipid mediators that control a variety of physiological and pathophysiological processes. They...     

柱前手性衍生化反相高效液相色谱法分离拉贝洛尔对映体

以乙酰葡萄糖异硫氰酸酯（GITC）作柱前手性衍生化试剂,用反相高效液相色谱法成功地分离了拉贝洛尔的两对对映异构体,并以荧光检测和紫外检测作对照,确认了4个衍生物的色谱峰。     

反相高效液相色谱法制备松果菊苷标准品

 利用反相制备高效液相色谱结合溶剂萃取、大孔吸附树脂柱色谱和葡聚糖凝胶LH 2 0柱色谱方法 ,从管花肉苁蓉的乙醇提取物中纯化制备了苯乙醇苷类化合物松果菊苷的标准品 ,纯度达到 98%以上。方法操作简便 ,重现性好 ,可用于松果菊苷及其他苯乙醇苷类化合物的大量制备。     

反相高效液相色谱法同时测定6种氟喹诺酮类药物

建立了一种反相高效液相色谱-荧光检测法同时测定血浆中6种氟喹诺酮类药物(FQS)的方法。考察了6种FQS的保留值与流动相组成及pH值的关系, 优化了色谱条件及样品前处理方法。确定了以Chira Dex为色谱柱、乙腈-甲醇-Britton-Robinson缓冲液(体积比为73∶7∶20, pH 5.7)为流动相的最佳条件。该法用于血浆样品中FQS的测定,其回收率高于99.0%。该法简便、快速、准确、灵敏度高、重现性好。     

反相高效液相色谱法测定嘌呤、嘧啶类化合物

用反相高效液相色谱法同时分离和测定了胞嘧啶、胸腺嘧啶、腺嘌呤、茶碱和咖啡因等５种结构相似的生物碱基。方法简便、快速,用于茶样中咖啡因的测定,标准加入回收率在９５．６９％～１０４．２０％之间。     

Normal- and Reversed-Phase Thin-Layer Chromatography of Amino Acids on Unconventional Supports with Mixed Mobile Phases Containing Dimethyl Sulfoxide

JPC – Journal of Planar Chromatography – Modern TLC - The chromatographic behavior of twenty-four amino acids has been studied on untreated and on triaryl phosphate (TAP)-impregnated...