Simultaneous Determination of Cilazapril and Hydrochlorothiazide in Tablets by Spectrophotometric Methods

Abstract

In this study, two new spectrophotometric methods were used for the simultaneous determination of cilazapril and hydrochlorothiazide in their binary mixture. In the first method, derivative spectrophotometry, dA/dδ values were measured at 242.8 nm and 282.8 nm for cilazapril and hydrochlorothiazide, respectively, in the first derivative spectra of their combination. The relative standard deviation of the method was found to be 0.77% for cilazapril and 0.24% for hydrochlorothiazide. In the second, the absorbancy ratio method, the quantification of cilazapril and hydrochlorothiazide was performed by using the absorbances read at 210.4 nm. 250.2 nm and 270.6 nm in the zero-order spectra of their mixture; relative standard deviations of the method were found to be 1.26 % and 0.81 % for cilazapril and hydrochlorothiazide, respectively. These two methods have been successfully applied to a tablet containing these drugs.     

Simultaneous Determination of Zofenopril and Hydrochlorothiazide in Tablets Using Derivative UV Spectrophotometry

A derivative UV-vis spectrophotometric procedure for the simultaneous determination of Zofenopril, a new angiotensin-converting enzyme inhibitor and Hydrochlorothiazide, a thiazide diuretic, in tablets is developed and applied. The procedure is based on the use of the fourth-derivative ultraviolet spectra, by utilizing the derivative peak amplitude. The calibration graph is found to be linear in the range of 5.0–40 μg mL^?1 for Zofenopril and 1.0–20 μg mL^?1 for Hydrochlorothiazide. The proposed method is simple, rapid and gives accurate and precise results.     

Simultaneous Determination of Valsartan and Hydrochlorothiazide in Tablets by High-Performance Liquid Chromatography

ABSTRACT

A method for the simultaneous determination of valsartan and hydrochlorothiazide in tablets is described. The procedure, based on the use of reversed-phase high-performance liquid chromatography, is linear in the concentration range 5.0-10.0 μg ml^?1 for valsartan and 0.5-2.0 μg ml^?1 for hydrochlorothiazide, is simple and rapid and allows accurate and precise results. The limit of detection was 1.0 μg ml^?1 for valsartan and 0.05 μg ml^?1 for hydrochlorothiazide.     

Simultaneous Determination of Hydrochlorothiazide and Propranolol Hydrochloride in Tablets by High-Performance Liquid Chromatography

Abstract

A simple and stability indicating HPLC procedure is described for the simultaneous determination of hydrochlorothiazide and propranolol hydrochloride in tablet formulations. Potential degradation products of both drugs and synthesis impurities of hydrochlorothiazide were separated, making the determination stability indicating for both drugs and selective for hydrochlorothiazide. All compounds were chromatographed on a cyanopropylsilane column, eluted with a 15:85 mixture of acetonitrile: 0.05 M ammonium phosphate (pH 3.0) and detected at 290 m. Excellent interlaboratory precision and recovery data were obtained. Linearity studies were carried out using peak area measurements. Detector response to the concentration of each drug was confirmed. The method was applied to dosage forms containing 25 mg of hydrochlorothiazide and 40 or 80 mg of propranolol hydrochloride.     

Densitometric Determination of Mebhydrolin Napadisylate in Tablets

JPC – Journal of Planar Chromatography – Modern TLC - A simple and rapid high-performance thin-layer chromatography (HPTLC) densitometric method has been developed for the determination...     

Simultaneous Densitometric TLC Analysis of Olmesartan Medoxomil and Hydrochlorothiazide in the Tablet Dosage Form

JPC – Journal of Planar Chromatography – Modern TLC - A simple, rapid, and selective densitometric thin-layer chromatographic (TLC) method has been established and validated for...     

Densitometric Determination of Desloratadine in Tablets,and Validation of the Method

JPC – Journal of Planar Chromatography – Modern TLC - A simple and rapid densitometric method has been developed for determination of desloratadine in tablets. After extraction of the...     

Simultaneous Spectrophotometric Determination of Hydrochlorothiazide and Pharmaceutical Preparations

Abstract

We developed three methods for the simultaneous determination of amiloride (AMI) and hydrochlorothiazide (HCT): zero-crossing, derivative quotient spectra with normalized divisor and multiple linear regression (MULTIC) methods. The two first methods use the derivative spectrophotometry, and the last one uses the absorbance measurement. The three methods were used to determine both compounds in synthetic mixtures and pharmaceutical preparations with errors less than 5% and 15%, respectively.     

Determination of Fluvoxamine and Moclobemide in Tablets by Densitometric and Videodensitometric TLC

JPC – Journal of Planar Chromatography – Modern TLC - New, simple, rapid, and accurate thin-layer chromatographic methods have been established for determination of fluvoxamine and...     

Simultaneous Densitometric Determination of Hydrocortisone Acetate and 2-Phenoxyethanol in Creams

JPC – Journal of Planar Chromatography – Modern TLC - A simple and rapid TLC-densitometric method has been developed for simultaneously determination of hydrocortisone acetate and...     

Simultaneous HPLC Analysis of Olmesartan and Hydrochlorothiazide in Combined Tablets and in vitro Dissolution Studies

A simple, rapid and reproducible HPLC method was developed and validated for the simultaneous determination of olmesartan (OLM) medoxomil and hydrochlorothiazide (HCT) in combined tablets. Chromatography was carried out on a 4.6 mm I.D × 200 mm, 5 μm cyano column with methanol–10 mM phosphoric acid containing 0.1% triethylamine (pH 2.5, 50:50 v/v) at a flow rate of 1.0 mL min⁻¹ and UV detector was set at 260 nm. Valsartan (VAL) was used as internal standard (IS). A linear response was observed in the range of 0.2–6 μg mL⁻¹ (r ² = 0.9998) for OLM and 0.1–4 μg mL⁻¹ (r ² = 0.9999) for HCT, respectively. The method showed good recoveries (99.56% for OLM and 99.48% for HCT) and the relative standard deviation (RSD) values for intra- and inter-day precision were 0.70–1.59 and 0.80–2.00% for OLM and 1.20–1.37 and 1.63–1.93% for HCT, respectively. The developed method was applied successfully for quality control assay of OLM and HCT in combined tablets and in vitro dissolution studies.     

Reversed-Phase High Performance Liquid Chromatographic and Thin Layer Chromatographic Methods for the Simultaneous Determination of Benazepril Hydrochloride and Hydrochlorothiazide in Cibadrex Tablets

ABSTRACT

Two procedures were developed for simultaneous determination of benazepril hydrochloride (I) and hydrochlorothiazide (II) in pure, laboratory made mixtures and in pharmaceutical dosage form “Cibadrex tablets® using reversed phase high performance liquid chromatographic and thin layer chromatographic methods.

For reversed phase HPLC, a new very sensitive, rapid, selective method was developed. The linearity ranges were 32-448 ng/20 μl and 40-560 ng/20 μl for benazepril hydrochloride and hydrochlorothiazide, respectively. The corresponding recoveries were 99.38 ± 1.526 and 99.2 ± 1.123.

The minimum detection limits were 7 ng/20 μl and 14 ng/20 μl for benazepril hydrochloride and hydrochlorothiazide respectively.

On the other hand, a new, simple, sensitive and fast thin layer chromatographic scanning densitometric method was developed for simultaneous determination of benazepril hydrochloride and hydrochlorothiazide using ethyl acetate: methanol: ammonia (85: 20: 10 v/v) as the developing system. The R_f values were 0.33 & 0.68 for benazepril hydrochloride and hydrochlorothiazide respectively. The minimum detection limit obtained was 0.12 μg/spot for benazepril hydrochloride and 0.24 μg/spot for hydrochlorothiazide. The mean percentage recoveries were 100.04 ± 1.102 and 99.31 ± 1.009 for benazepril hydrochloride and hydrochlorothiazide respectively.

The two proposed methods were simple, precise, sensitive and could be successfully applied for the determination of pure, laboratory made mixtures and pharmaceutical dosage forms. The results obtained were compared with those obtained by A ^1%.     

Simultaneous Densitometric Determination of Ivermectin and Albendazole by High-Performance Thin-Layer Chromatography

JPC – Journal of Planar Chromatography – Modern TLC - This paper describes a validated high-performance thin-layer chromatography (HPTLC) method for the simultaneous determination of...     

Simultaneous Densitometric Determination of Rifampicin and Isoniazid by High-Performance Thin-Layer Chromatography

JPC – Journal of Planar Chromatography – Modern TLC - High-performance thin-layer chromatography (HPTLC) on silica gel 60 F254 TLC plates, with ethyl acetate-methanol-acetone-acetic...     

时间分辨-后化学发光法同时测定阿米卡星和氢氯噻嗪

根据阿米卡星、氢氯噻嗪在过氧化单硫酸盐(PMS)-鲁米诺(Luminol)体系中的化学发光反应的动力学性质有着明显的差异性,建立了同时测定阿米卡星和氢氯噻嗪新的时间分辨后化学发光的方法。在PMS-鲁米诺体系中,氢氯噻嗪化学发光反应较快,1 s达到最大值,峰尖锐;而阿米卡星化学发光反应较慢,37.9 s后达到最大值,峰平缓,且其动力学曲线呈现出随时间分开的两个独立的发光峰,互不干扰。结果表明;氢氯噻嗪和阿米卡星的线性范围分别为8.0×10-5～4.0×10-3g/L和8.0×10-4～4.0×10-2g/L;11次平行测定4.0×10-4g/L氢氯噻嗪溶液和4.0×10-3g/L阿米卡星溶液的化学发光强度相对标准偏差分别为2.4%和3.7%。本方法测定氢氯噻嗪和阿米卡星的检出限分别为2.0×10-5和2.0×10-4g/L。     

Simultaneous Densitometric Determination of Aspirin and Omeprazole by High-Performance Thin-Layer Chromatography

JPC – Journal of Planar Chromatography – Modern TLC - A new, simple, accurate, and precise high-performance thin-layer chromatographic (HPTLC) method has been developed for the...     

离子色谱法测定复方卡托普利片中卡托普利和氢氯噻嗪的含量

建立离子色谱法测定复方卡托普利片中卡托普利和氢氯噻嗪含量的方法。在色谱柱为IonPacSCS1(4mm×250mm),保护柱为IonPacSCG1(4mm×50mm),淋洗液为甲烷磺酸(4mmol/L)+乙腈(26%),流速1.0mL/min,柱温35℃,进样量7μL,紫外检测波长215nm的条件下,测定结果表明,卡托普利和氢氯噻嗪在0.5～25μg/mL范围内线性关系良好,相关系数r分别为0.9997、0.9999,回收率分别为98.11%、94.24%。方法操作简单,灵敏度高、准确、重现性好,可用于复方卡托普利片的质量控制。     

HPLC and UPLC Methods for the Simultaneous Determination of Enalapril and Hydrochlorothiazide in Pharmaceutical Dosage Forms

High efficiency and less elution are the basic requirements of high-speed chromatographic separation. In this study, a new gradient reverse phase chromatographic methods were developed using HPLC and UPLC systems for simultaneous determination of enalapril maleate (ENL) and hydrochlorothiazide (HCZ) in pharmaceutical dosage forms. The chromatographic separations of ENL and HCZ were achieved on a Waters μ-Bondapak C 18, (300 × 3.9 mm, 10 μm) and Waters Acquity BEH C18 (100 × 2.1 mm, 1.7 μm) columns for HPLC within 5.30 min and UPLC within a short retention time of 1.95 min, respectively. A linear response was observed over the concentration range 0.270–399 μg mL⁻¹ of ENL, 0.260–399 μg mL⁻¹ of HCZ for HPLC system and 0.270–399 μg mL⁻¹ of ENL and 0.065–249 μg mL⁻¹ of HCZ for UPLC system. Also, limit of detection for ENL was 1.848 ng mL⁻¹ and 31.477 ng mL⁻¹ for HCZ, 2.804 ng mL⁻¹ for ENL and 2.943 ng mL⁻¹ for HCZ using HPLC and UPLC, respectively. The proposed methods were validated according to ICH guideline with respect to precision, accuracy, and linearity. Forced degradation studies were also performed for both compounds in bulk drug samples to demonstrate the specificity and stability indicating power of the HPLC method. Comparison of system performance with conventional HPLC was made with respect to analysis time, efficiency, and resolution.

     

HPLC and UPLC Methods for the Simultaneous Determination of Enalapril and Hydrochlorothiazide in Pharmaceutical Dosage Forms

High efficiency and less elution are the basic requirements of high-speed chromatographic separation. In this study, a new gradient reverse phase chromatographic methods were developed using HPLC and UPLC systems for simultaneous determination of enalapril maleate (ENL) and hydrochlorothiazide (HCZ) in pharmaceutical dosage forms. The chromatographic separations of ENL and HCZ were achieved on a Waters μ-Bondapak C 18, (300 × 3.9 mm, 10 μm) and Waters Acquity BEH C18 (100 × 2.1 mm, 1.7 μm) columns for HPLC within 5.30 min and UPLC within a short retention time of 1.95 min, respectively. A linear response was observed over the concentration range 0.270–399 μg mL^?1 of ENL, 0.260–399 μg mL^?1 of HCZ for HPLC system and 0.270–399 μg mL^?1 of ENL and 0.065–249 μg mL^?1 of HCZ for UPLC system. Also, limit of detection for ENL was 1.848 ng mL^?1 and 31.477 ng mL^?1 for HCZ, 2.804 ng mL^?1 for ENL and 2.943 ng mL^?1 for HCZ using HPLC and UPLC, respectively. The proposed methods were validated according to ICH guideline with respect to precision, accuracy, and linearity. Forced degradation studies were also performed for both compounds in bulk drug samples to demonstrate the specificity and stability indicating power of the HPLC method. Comparison of system performance with conventional HPLC was made with respect to analysis time, efficiency, and resolution.     

Simultaneous RP-LC Determination of Losartan Potassium, Ramipril, and Hydrochlorothiazide in Pharmaceutical Preparations

A simple, rapid, and precise reversed-phase high-performance liquid chromatographic method has been developed for simultaneous determination of losartan potassium, ramipril, and hydrochlorothiazide. The three drugs were separated on a 150 mm × 4.6 mm i.d., 5 μm particle, Cosmosil C₁₈ column. The mobile phase was 0.025 m sodium perchlorate–acetonitrile, 62:38 (v/v), containing 0.1% heptanesulphonic acid, pH adjusted to 2.85 with orthophosphoric acid, at a flow rate of 1.0 mL min⁻¹. UV detection was performed at 215 nm. The method was validated for linearity, accuracy, precision, and limit of quantitation. Linearity, accuracy, and precision were acceptable in the ranges 35–65 μg mL⁻¹ for losartan, 1.75–3.25 μg mL⁻¹ for ramipril, and 8.75–16.25 μg mL⁻¹ for hydrochlorothiazide.