Oscillatory dynamics in a gene regulatory network mediated by small RNA with time delay

A genetic regulatory network mediated by small RNA with two time delays is investigated. We show by mathematical analysis and simulation that time delays can provide a mechanism for the intracellular oscillator. By linearizing the system at the positive equilibrium and analyzing the associated characteristic equation, the asymptotic stability of the positive equilibrium is investigated and Hopf bifurcations are demonstrated. Furthermore, the direction of Hopf bifurcation and the stability of the bifurcating periodic solutions are determined by the normal form theory and the center manifold theorem for functional differential equations. Finally, some numerical simulations are carried out for illustrating the theoretical results.     

Spatial Coherence of the Heat Release Fluctuations in Turbulent Jet and Swirl Flames

The noise generation of turbulent flames is governed by temporal changes of the total flame volume due to local heat release fluctuations. On the basis of the wave equation an expression for the relationship between the acoustic power and the heat release fluctuations is derived and a correlation function is obtained which reveals that the sound pressure level of flames is governed by the spatial coherence. Noise models rely on precise coherence information in terms of characteristic length scales, which are the measure of the acoustic efficiency of the flame. Since the published length scale information is scarce and inconsistent, length scales were measured for a number of laboratory flames using two measurement techniques developed for this purpose: A planar LIF-system with a repetition rate of 1 kHz acquires the instantaneous flame front position and heat release, whereas two chemiluminescence probes with an optics confining the measurement volume to a line of sight provide further spatial correlation data. For all flames investigated the length scales are smaller than the height of the burner exit annulus and they are of the order of the local flame brush thickness. Using the measured length scales, the coherent volume and the efficiency of the noise generation are calculated, which are three orders of magnitude higher than measured. However, the proper order of magnitude is obtained, if only the measured fluctuating part of the thermal power is used in the model and if the periodic formation of local zones with heat release overshoot and deficit are properly incorporated.     

Research on parametric resonance in a stochastic van der Pol oscillator under multiple time delayed feedback control

Analytical derivations and numerical calculations are employed to gain insight into the parametric resonance of a stochastically driven van der Pol oscillator with delayed feedback. This model is the prototype of a self-excited system operating with a combination of narrow-band noise excitation and two time delayed feedback control. A slow dynamical system describing the amplitude and phase of resonance, as well as the lowest-order approximate solution of this oscillator is firstly obtained by the technique of multiple scales. Then the explicit asymptotic formula for the largest Lyapunov exponent is derived. The influences of system parameters, such as magnitude of random excitation, tuning frequency, gains of feedback and time delays, on the almost-sure stability of the steady-state trivial solution are discussed under the direction of the signal of largest Lyanupov exponent. The non-trivial steady-state solution of mean square response of this system is studied by moment method. The results reveal the phenomenon of multiple solutions and time delays induced stabilization or unstabilization, moreover, an appropriate modulation between the two time delays in feedback control may be acted as a simple and efficient switch to adjust control performance from the viewpoint of vibration control. Finally, theoretical analysis turns to a validation through numerical calculations, and good agreements can be found between the numerical results and the analytical ones.     

猪传染性胃肠炎病毒聚合酶基因反义RNA对病毒的抑制作用

通过PCR扩增出猪传染性胃肠炎病毒(TGEV)聚合酶部分片段,反向插入逆转录病毒表达载体pLXSN中,用脂质体法将重组质粒plxas-pol转染PA317细胞,经抗生素G418筛选出稳定的产毒细胞克隆,分别扩大培养后,取其上清液感染小鼠成纤维细胞NIH3T3,细胞克隆产生的重组病毒效价达9.0×105CFU/mL。用高效价假病毒感染IBRS2细胞,再经抗生素G418筛选,提取细胞克隆总RNA,经RT-PCR证明plxas-pol整合入IBRS2细胞。以TGEV感染IBRS2细胞和具有抗性的IBRS2细胞所产生的细胞病变为指标,证明该反义RNA对病毒有明显抑制作用,抑制率约为80%。用2×103和4×102TCID50/mL剂量的TGEV感染时,引起细胞死亡的时间分别为21 h和27 h,与对照组相比,抗性细胞系可明显延迟因病毒感染引起细胞死亡的时间。     

小口径膛口射流噪声的数值模拟

为了研究小口径武器的膛口气动噪声特性,采用CFD-CAA耦合算法对7.62 mm枪的射流噪声场进行了数值模拟。由于膛口流场结构复杂,在目前的计算发展水平下还不足以采用CAA直接法,因而本文中采用混合方法,即首先采用CFD方法计算7.62 mm枪的膛口流场,然后利用所得结果,采用声学方程计算射流噪声,具体为膛口近场采用LES进行计算,远场声场采用FW-H声拟法计算。通过对比验证实验,验证了该计算方法的可行性。然后,对7.62 mm枪射流噪声进行了数值模拟,分析了噪声指向性,绘制了声压级云图。研究表明：在本文的计算条件下,射流噪声强度主要集中在近膛口区域;且射流最大噪声主要分布在与轴线方向成30°~60°范围内。

     

Sub-harmonic resonance in a nearly pre-loaded mechanical oscillator

In this paper, the sub-harmonic resonance of a single degree of freedom system with a nearly preloaded non-linearity (gain-changing clearance) is studied. First, a new perturbation approach is incorporated in the traditional multi-term harmonic balance method to calculate the sub-harmonic resonance. Our approach significantly reduces the work as it computes the sub-harmonic responses with just one run. Initial guesses in the vicinity of sub-harmonic regime are relaxed compared to prior approaches. Second, a parametric study is conducted to examine the occurrence and characteristics of sub-harmonic resonance. The possibility of the sub-harmonic occurrence increases with an increase of dynamic excitation or the stiffness ratio. For instance, our analysis shows that the sub-harmonic resonance typically occurs when the mean load is close to the stiffness transition point. In the extreme case, a very small excitation would generate a sub-harmonic resonance. With a higher mean load, the resonant peak appears at lower frequencies as a result of the reduced equivalent stiffness. Finally, our analytical formulation is successfully validated using numerical integration results.     

组合荷载下超大群桩受力变形模型试验研究

研究表皮生长因子(EGF)2,4,7,14d处理后鼠小肠所发生的生物力学重建. 通 过双轴试验(膨胀与轴向拉伸组合)，用最小二乘法计算了近于在体状态下环向、纵向及交 叉向增量杨氏模量. 各组环向和纵向模量均不相等，即正常状态和EGF处理后的小肠是各向 异性的. 在EGF处理期间，各向的杨氏模量随时间变化(P<0.05). 在EGF处理的最 初7d中，环向模量减小，在处理14d后，又恢复到对照组的水平. 增量模量介 于17.4kPa与24.2kPa 之间. 纵向模量的值介于22.9kPa与32.4kPa之间，在处理4d后，比对照值显著增大(P<0.02). 交叉模量的值介于4.7kPa与6.6kPa之间，在最初EGF处理的4d中降低，此后增加并 于第7d达到最大值. 可以得出结论，肠壁的力学性质呈各向异性且在EGF处理期间会发生重建.     

Quasi-periodic and chaotic behaviour of a two-degree-of-freedom impact system in a strong resonance case

A two-degree-of-freedom system contacting a single stop is considered. Quasi-periodic and chaotic behavior of the system in a strong resonance case is investigated by theoretical analysis and numerical simulation This work was supported by the National Natural Science Foundation of China(19672052).     

Vibration control for the primary resonance of the van der Pol oscillator by a time delay state feedback

We investigate the primary resonance of an externally excited van der Pol oscillator under state feedback control with a time delay. By means of the asymptotic perturbation method, two slow-flow equations on the amplitude and phase of the oscillator are obtained and external excitation-response and frequency-response curves are shown. We discuss how vibration control and high amplitude response suppression can be performed with appropriate time delay and feedback gains. Moreover, energy considerations are used in order to investigate existence and characteristics of limit cycles of the slow-flow equations. A limit cycle corresponds to a two-period modulated motion for the van der Pol oscillator. We demonstrate that appropriate choices for the feedback gains and the time delay can exclude the possibility of modulated motion and reduce the amplitude peak of the primary resonance. Analytical results are verified with numerical simulations.     

Application of reconstitution multiple scale asymptotics for a two-to-one internal resonance in Magnetic Resonance Force Microscopy

In this paper we formulate an initial-boundary-value-problem describing the three-dimensional motion of a cantilever in a Magnetic Resonance Force Microscopy setup. The equations of motion are then reduced to a modal dynamical system using a Galerkin ansatz and the respective nonlinear forces are expanded to cubic order. The direct application of the asymptotic multiple scales method to the truncated quadratic modal system near a 2:1 internal resonance revealed conditions for periodic and quasiperiodic energy transfer between the transverse in-plane and out-of-plane modes of the MRFM cantilever. However, several discrepancies are found when comparing the asymptotic results to numerical simulations of the full nonlinear system. Therefore, we employ the reconstitution multiple scales method to a modal system incorporating both quadratic and cubic terms and derive an internal resonance bifurcation structure that includes multiple coexisting in-plane and out-of-plane solutions. This structure is verified and reveals a strong dependency on initial conditions in which orbital instabilities and complex out-of-plane non-stationary motions are found. The latter are investigated via numerical integration of the corresponding slowly-varying evolution equations which reveal that breakdown of quasiperiodic tori is associated with symmetry-breaking and emergence of irregular solutions with a dense spectral content.     

An experimental and analytical study of autoparametric resonance in a 3DOF model of cable-stayed-beam

Autoparametric interaction and the associated phenomenon of amplitude saturation are experimentally observed in a physical model of cable-and-beam structure. In this system, the horizontal beam is fixed at one end and supported at the other end by an inclined taut cable. The longitudinal axes of beam and cable are in a vertical plane. Three natural frequencies of the system are approximately of the ratio 1:1:2. This is a combination of two conditions that are very likely to occur in relatively long-span, multi-stay-cable bridges, namely, 1:1 tuning and 1:2 superharmonic tuning. While the beam is vertically excited with sufficiently large force near a primary resonance, the cable vibrates horizontally at half of excitation frequency. The beam also vibrates horizontally at half-frequency, as well as vertically. As the vertical excitation on the bean is further increased in amplitude, the vertical vibration amplitude gets saturated instead of increasing proportionately. A 3DOF analytical model of the structure is also derived, where the finite motion of the cable introduces geometric nonlinearities in quadratic and cubic forms. The system parameters having been carefully measured from the experimental model, steady-state solutions of the coupled nonlinear equations of motion are obtained, by the perturbation method of multiple time scales. Agreement between experimental observation and analytical prediction is very good, both qualitatively and quantitatively. Very good agreement is found also in the case of horizontal excitation of the beam, where effects of linear and nonlinear interaction are apparent.     

一类冲击振动系统在强共振条件下的亚谐分叉与Hopf分叉

通过理论分析和数值仿真,研究了一类二维冲击振动系统在一种强共振条件下的Hopf分叉与亚谐分叉。分析并证实了该类系统在此共振条件下可由稳定的周期1 1振动分叉为周期4 4振动或概周期振动,讨论了亚谐振动和概周期振动向混沌运动的演化过程。     

Analysis of guided waves with a nonlinear boundary condition caused by internal resonance using the method of multiple scales

We theoretically investigated the cumulative nonlinear guided waves caused by internal resonance, using the method of multiple scales (MMS), which can construct better approximations to the solutions of perturbation problems. In this study, we consider nonlinearity only on the boundary instead of material nonlinearity or geometric nonlinearity. We showed nonlinear effects on the amplitudes of a lower mode and a higher mode depending on the propagation length. Also, we examined effects of wavenumber detuning from a phase matching condition of the two modes. If the wavenumber detuning is exactly equal to zero, the mechanical energy of the lower mode is transferred through nonlinear coupling to the energy of the higher mode, unilaterally. However, if a wavenumber detuning is not equal to zero, amplitude of the two modes change in a cyclic fashion during wave propagation. The amount of this amplitude variation and its cycle length are determined by the eigenfunctions of the two modes, the nonlinear parameter and the wavenumber detuning.     

The near tip fields and temperature distribution around a crack in a body of hardening material containing small damage

In this paper, the following conclusions are reached: The influence of damage on the stress and strain feilds can be neglected in an asymptotic sense for the solutions of damage field in a plastic solid containing small damage. The formulation of the problem is simplified with an uncoupled approach. Based on experimental results of plastic damage, most of the damage in the material are considered as small damage with the critacal damage variable ω _c ≪1. Using this approach, closed form expressions of the near tip damage fields for mode III, mode I and the temperature distribution induced by plastic dissipation in a hardening material containing damage are deduced. We point out that the temperature distribution in the process zone is strongly dependent on the damage of materials even for the small damage case. The results of the predicted value of the temperature rise near the tip region ignoring the damage effect is appreciably higher than the observed data. The main reason of this discrepancy is the presence of damage dissipation and the fact that its influence on the calculation of plastic dissipation have not been appropriately taken account of. The calculation is improved by taking into account the damage effect on the temperature rise, then theT _max value is in better accord with the experimental value. The project supported by the National Natural Science Foundation of China.     

Numerical and experimental investigation of flow-acoustic resonance of side-by-side cylinders in a duct

The phenomenon of flow-excited acoustic resonance is a design concern in many engineering applications, especially when wakes of bluff bodies are encountered in ducts, piping systems, heat exchangers, and other confined systems. In this paper, the case of self-excited acoustic resonance of two side-by-side cylinders in a duct with cross-flow is investigated both numerically and experimentally for a single spacing ratio of T/D=2.5, where D is the diameter of the cylinders and T is the centre-to-centre distance between them. The numerical investigation is performed using a finite-volume method at a Reynolds number of 3.0×10⁴ to simulate the unsteady flow field, which is then coupled with an imposed resonant sound field of the first acoustic cross-mode of the duct calculated through the use of Finite Element Analysis (FEA). The experimental investigation has been performed using phase-locked Particle Image Velocimetry (PIV) of the flow field during the occurrence of a self-excited acoustic resonance condition in the duct. The results of both methods reveal that the flow-excited acoustic resonance produces a strong oscillatory flow pattern in the cylinder wakes, with strong in-phase vortex shedding being synchronized by the acoustic resonance. The distribution and strength of the aeroacoustic sources and sinks within the flow field have been computed by means of Howe׳s theory of aerodynamic sound for both the experimental and numerical cases, with the results of the two methods comparing favourably, showing comparable trends in the oscillating flow fields, and very similar trends in the distribution of net acoustic power.     

Gas bubble oscillations in a fluid in the presence of 2:1 resonance between the radial and deformation oscillation frequencies

Small nonlinear oscillations of an ellipsoidal bubble in a fluid in the presence of 2:1 frequency resonance between the radial and ellipsoidal modes are considered. The equations of motion are reduced to Hamiltonian form. The quadratic and cubic terms are taken into account in the expansion of the Hamiltonian. The Hamilton function is transformed to the normal form using the invariant normalization method in the first approximation. This makes it possible to construct an analogy between the system considered and the well-known problem of a pendulous spring. The radial and ellipsoidal bubble oscillation modes correspond to the vertical and horizontal coordinates of a material point, respectively. In the absence of resonance the solution of the nonlinear equations differs from the solution of the linear equations by only a small (quadratic in the amplitude) change in the oscillation frequency. In the resonance case the radial and ellipsoidal oscillation modes periodically change places and the energy of one mode is converted into that of the other. The interest in the system in resonance is associated with precisely this fact. The question of the dissipation effect in real media is considered. The decay rate depends significantly on the physical properties of the material and, in certain special cases, can be small enough for the energy transfer effect to manifest itself.     

Measurements of the dynamic lift force acting on a circular cylinder in cross-flow and exposed to acoustic resonance

A piezoelectric transducer is developed to perform direct measurements of the dynamic lift force acting on a circular cylinder in cross-flow, in the presence and absence of acoustic resonance. Details of the force transducer design are presented in the paper. The dynamic lift force is measured for a single cylinder with two different diameters, D=12.7 and 15.8 mm. During the tests, the first transverse acoustic mode of the duct housing the cylinder is self-excited. The fluctuating pressure on the top wall of the duct is measured simultaneously with the dynamic lift force. In the absence of acoustic resonance, the measured dynamic lift coefficients agree favorably with those reported in the literature. However, when the acoustic resonance is initiated, the dynamic lift experiences a drastic increase in amplitude associated with abrupt changes in the phase between the lift force and the acoustic pressure. A methodology to extract the hydrodynamic lift component from the total lift measured during acoustic resonance is also proposed. The hydrodynamic lift force is then decomposed into in-phase and out-of-phase components, with respect to the resonant sound pressure. This decomposition procedure provides new insights into the nature of the aeroacoustic sources in the cylinder wake. The proposed methodology, together with the test results provide a general design approach to assess the increase in the dynamic fluid loading on bluff bodies in cross-flow due to the excitation of acoustic resonance.